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The map label for this gene is gloA [H]

Identifier: 213158141

GI number: 213158141

Start: 2941605

End: 2942006

Strand: Reverse

Name: gloA [H]

Synonym: AB57_2855

Alternate gene names: 213158141

Gene position: 2942006-2941605 (Counterclockwise)

Preceding gene: 213158142

Following gene: 213158140

Centisome position: 72.63

GC content: 39.8

Gene sequence:

>402_bases
ATGCGAATGCTTCATACAATGTTGCGAGTAGGCAATTTAGAACAATCTTTAAAGTTCTATACCGAAGTATTAGGGATGAA
GTTACTTCGTAAGCGTGATTATGAAGAAGGTCGCTTTACACTGGCATTTGTTGGTTATGGCGATGAAGAAAATAATACCG
TGCTTGAACTCACTCATAACTGGGATACCTCAAGTTATGATTTAGGAAATGGCTATGGTCACATTGCGATTGGTGTTGAA
GATGCTTATAAAGCATGTGAAGAAATCAAAGCACGTGGTGGTAAAGTCGTTCGTGAAGCTGGCCCAATGAAGGGTGGTGT
AACGGTGATTGCATTTGTTGAAGATCCGGATGGCTACAAAGTCGAGCTTATTCAACAAGACGCAAATGCTAGAAATAATT
AA

Upstream 100 bases:

>100_bases
CCACAATGATCGGGATTCCAATCTTAATTTTAGTTGTTTGGGTTGGAAAACTGCTTAAAGATCATCAAACACATGACATA
GAAAAGGCAGGTGAATAAAC

Downstream 100 bases:

>100_bases
ATAAATGACTCAGGCGCTTTAAGATTTAGGTCTTAAAAGTCTAGCTTTATTTTTATAGCCCAGTATGATGAAGGCTTGAC
TAAATGGTCAATTTATCAAC

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MRMLHTMLRVGNLEQSLKFYTEVLGMKLLRKRDYEEGRFTLAFVGYGDEENNTVLELTHNWDTSSYDLGNGYGHIAIGVE
DAYKACEEIKARGGKVVREAGPMKGGVTVIAFVEDPDGYKVELIQQDANARNN

Sequences:

>Translated_133_residues
MRMLHTMLRVGNLEQSLKFYTEVLGMKLLRKRDYEEGRFTLAFVGYGDEENNTVLELTHNWDTSSYDLGNGYGHIAIGVE
DAYKACEEIKARGGKVVREAGPMKGGVTVIAFVEDPDGYKVELIQQDANARNN
>Mature_133_residues
MRMLHTMLRVGNLEQSLKFYTEVLGMKLLRKRDYEEGRFTLAFVGYGDEENNTVLELTHNWDTSSYDLGNGYGHIAIGVE
DAYKACEEIKARGGKVVREAGPMKGGVTVIAFVEDPDGYKVELIQQDANARNN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=152, Percent_Identity=39.4736842105263, Blast_Score=102, Evalue=1e-22,
Organism=Homo sapiens, GI217330598, Length=92, Percent_Identity=36.9565217391304, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1787940, Length=131, Percent_Identity=61.0687022900763, Blast_Score=174, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17552228, Length=141, Percent_Identity=31.2056737588652, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323639, Length=138, Percent_Identity=42.7536231884058, Blast_Score=108, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=37.5, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19920404, Length=142, Percent_Identity=29.5774647887324, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14950; Mature: 14950

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLHTMLRVGNLEQSLKFYTEVLGMKLLRKRDYEEGRFTLAFVGYGDEENNTVLELTHN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WDTSSYDLGNGYGHIAIGVEDAYKACEEIKARGGKVVREAGPMKGGVTVIAFVEDPDGYK
CCCCCEECCCCCEEEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCCCE
VELIQQDANARNN
EEEEECCCCCCCC
>Mature Secondary Structure
MRMLHTMLRVGNLEQSLKFYTEVLGMKLLRKRDYEEGRFTLAFVGYGDEENNTVLELTHN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WDTSSYDLGNGYGHIAIGVEDAYKACEEIKARGGKVVREAGPMKGGVTVIAFVEDPDGYK
CCCCCEECCCCCEEEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCCCE
VELIQQDANARNN
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]