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The map label for this gene is gcvA [H]

Identifier: 213158133

GI number: 213158133

Start: 2930286

End: 2931212

Strand: Reverse

Name: gcvA [H]

Synonym: AB57_2845

Alternate gene names: 213158133

Gene position: 2931212-2930286 (Counterclockwise)

Preceding gene: 213158134

Following gene: 213158129

Centisome position: 72.37

GC content: 38.94

Gene sequence:

>927_bases
ATGAAAATTAAGCCATTGCCGCCAATGAACAGCTTAATTGTATTCGAGGCAGCCGCACGCCACCTCAGTTTTACAAAAGC
TGCAAATGAATTGAATGTTACTCAAGGCGCTATCAGTCGACAAATTCGACAGCTCGAAGAATATTTAGGTAAGGAATTAT
TCTTAAGAGCTAATCGAAATATTTCCCTAACTCCTACCGGACTCCAATACTATCAAACGATCTATAGTGCTTTATTAGAT
GTTGCCCAAGCTACTGCTGAAATCAAGAAATGGCAAGGAGAGCATCAAATAACTGTGGCTACGACAAATGCAATGGCTGC
TCTTTGGTTATTACCGAAAGTGGCTGCTTTTCAACAAAATAATGAGGGCGTAGATTTAAGAATTTTAACGACAGACAATA
TTTCAGATCTGCACAAAATGGACTTCGATTTGGCATTATTTTATTGCCGTGTTCCTCCAGCAGGTATGAGAGTTACTACT
TTATTTCCTGAAGAGGTTTTTCCTGTTTGTAGTCCAAATTATCTTGCTCAATTTAAAGAAGATAGTTCTCCAGAAGAGAT
TTTTGGAAAAGCTCTATTATATCTAGATGATGCTCAAAAAGATTGGATTACTTGGTCAGAGTGGTTTGAAGCAGTAAATT
ATCCTATTGTAAAACCAAAAAATAGGGTGAATATCAATAACTATCCAATGCTTTTACAGGCGGCAATTAATGGTCAGGGG
GTGGCTTTGGCGTGGGGTTCACTCGTGGATGATTATTTACAAAGTGGTGCTTTAGTTAGACCTGTTGAGACTGTTCTTCG
TACTCAAGCAAATTTCAGTATGCTGGAACCAGCAGATAGGGGAGTTATTCCTTCTAGTGTTAAGCATTTCCGCAGTTGGC
TGCTAGATCAATTACCAGGCCAAGTTGGGCAAAAGGGTCTAAATTAA

Upstream 100 bases:

>100_bases
ATTAGTATGTTCAAAAGACAATTTTCATAGTAAATAGCGGAATAAAAGAACTCGAATTGGAAAAGAGTTTTTAAGGAAAT
GGGACAAATTAGGAGTGTCC

Downstream 100 bases:

>100_bases
TTTTAAGTAATAATTTTCTCTTGAAAACTAGGAATTTTGATATTTCTAGTTTTCAAGACCTTCAATTAATTTGATAAAAG
CTTAGTTTCAAAAGGATATA

Product: glycine cleavage system transcriptional activator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKIKPLPPMNSLIVFEAAARHLSFTKAANELNVTQGAISRQIRQLEEYLGKELFLRANRNISLTPTGLQYYQTIYSALLD
VAQATAEIKKWQGEHQITVATTNAMAALWLLPKVAAFQQNNEGVDLRILTTDNISDLHKMDFDLALFYCRVPPAGMRVTT
LFPEEVFPVCSPNYLAQFKEDSSPEEIFGKALLYLDDAQKDWITWSEWFEAVNYPIVKPKNRVNINNYPMLLQAAINGQG
VALAWGSLVDDYLQSGALVRPVETVLRTQANFSMLEPADRGVIPSSVKHFRSWLLDQLPGQVGQKGLN

Sequences:

>Translated_308_residues
MKIKPLPPMNSLIVFEAAARHLSFTKAANELNVTQGAISRQIRQLEEYLGKELFLRANRNISLTPTGLQYYQTIYSALLD
VAQATAEIKKWQGEHQITVATTNAMAALWLLPKVAAFQQNNEGVDLRILTTDNISDLHKMDFDLALFYCRVPPAGMRVTT
LFPEEVFPVCSPNYLAQFKEDSSPEEIFGKALLYLDDAQKDWITWSEWFEAVNYPIVKPKNRVNINNYPMLLQAAINGQG
VALAWGSLVDDYLQSGALVRPVETVLRTQANFSMLEPADRGVIPSSVKHFRSWLLDQLPGQVGQKGLN
>Mature_308_residues
MKIKPLPPMNSLIVFEAAARHLSFTKAANELNVTQGAISRQIRQLEEYLGKELFLRANRNISLTPTGLQYYQTIYSALLD
VAQATAEIKKWQGEHQITVATTNAMAALWLLPKVAAFQQNNEGVDLRILTTDNISDLHKMDFDLALFYCRVPPAGMRVTT
LFPEEVFPVCSPNYLAQFKEDSSPEEIFGKALLYLDDAQKDWITWSEWFEAVNYPIVKPKNRVNINNYPMLLQAAINGQG
VALAWGSLVDDYLQSGALVRPVETVLRTQANFSMLEPADRGVIPSSVKHFRSWLLDQLPGQVGQKGLN

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=301, Percent_Identity=37.5415282392027, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1788706, Length=259, Percent_Identity=31.2741312741313, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786448, Length=300, Percent_Identity=30.3333333333333, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786401, Length=305, Percent_Identity=25.2459016393443, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=23.1543624161074, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=22.5165562913907, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790262, Length=240, Percent_Identity=24.5833333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=23.828125, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=29.6969696969697, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34614; Mature: 34614

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKPLPPMNSLIVFEAAARHLSFTKAANELNVTQGAISRQIRQLEEYLGKELFLRANRN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHEEEEECCC
ISLTPTGLQYYQTIYSALLDVAQATAEIKKWQGEHQITVATTNAMAALWLLPKVAAFQQN
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCC
NEGVDLRILTTDNISDLHKMDFDLALFYCRVPPAGMRVTTLFPEEVFPVCSPNYLAQFKE
CCCEEEEEEECCCHHHHHHHCHHEEEEEEECCCCCCEEEEECHHHHCCCCCCHHHHHCCC
DSSPEEIFGKALLYLDDAQKDWITWSEWFEAVNYPIVKPKNRVNINNYPMLLQAAINGQG
CCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCE
VALAWGSLVDDYLQSGALVRPVETVLRTQANFSMLEPADRGVIPSSVKHFRSWLLDQLPG
EEEEHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
QVGQKGLN
HHHHCCCC
>Mature Secondary Structure
MKIKPLPPMNSLIVFEAAARHLSFTKAANELNVTQGAISRQIRQLEEYLGKELFLRANRN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHEEEEECCC
ISLTPTGLQYYQTIYSALLDVAQATAEIKKWQGEHQITVATTNAMAALWLLPKVAAFQQN
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCC
NEGVDLRILTTDNISDLHKMDFDLALFYCRVPPAGMRVTTLFPEEVFPVCSPNYLAQFKE
CCCEEEEEEECCCHHHHHHHCHHEEEEEEECCCCCCEEEEECHHHHCCCCCCHHHHHCCC
DSSPEEIFGKALLYLDDAQKDWITWSEWFEAVNYPIVKPKNRVNINNYPMLLQAAINGQG
CCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCE
VALAWGSLVDDYLQSGALVRPVETVLRTQANFSMLEPADRGVIPSSVKHFRSWLLDQLPG
EEEEHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
QVGQKGLN
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]