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The map label for this gene is recE [H]

Identifier: 213158024

GI number: 213158024

Start: 2805280

End: 2806131

Strand: Direct

Name: recE [H]

Synonym: AB57_2735

Alternate gene names: 213158024

Gene position: 2805280-2806131 (Clockwise)

Preceding gene: 213158023

Following gene: 213158025

Centisome position: 69.26

GC content: 40.96

Gene sequence:

>852_bases
ATGAATGCGCCAGTTTTGGTACATAACATGTCGAATGCAGCGTATCACGCACATTCAGCTGTTAGTAGCTCACAGCTTAA
AACCATTCTGCGTTCTCCTGCACACTTCTTTGCTGAGCACATGAGTGGTAAGGAACACAAGCAGACTACTGCAATGGCGC
TTGGTACTGCGGTTCATGTTCTATTTCTTGAACCAGAAGTTTTTAACGATGAAGTTGCAATCGAGCCAATCGTTAATAAA
CGAACAAATGTAGGTAAAGAAGCTATAGCAAAGTTCTTACAGGACAATGCAAGCAAGACAATCATTACCGAAGAACAGTA
CCAGGCAGCTGCTAAAGCTGCGGAAGCAATGAAACGCCACCCTATGTACAACATGATTTTATCAGGTGGTATTCGTGAAG
CTTCGATCTTTTTTGATGATGAAGAAACAGGTCTTGAATGTCGTATTCGCCCTGATTGGCATGTAGCACCTGAAACAAGT
GAATATTTCCCTAACGGGTTAATTGTAGACATCAAAAAGACAACGGATGCGCGTGCAAATGCATTTTCAAGAAGTTGTCA
AAACTACGATTACTCACTTTCAGCGGCTATGTATATCAATGGATACAAGGCTTATTACGGTGATGAATACAACCCTTCTT
TCCTATTTTTTGCAGTAGAAGAAGACGATCCGCATGAGTCAATCATCTATTACGCATCAGATGAAATGCTGTTTATTGGT
GAGCAGAAACGCCGATCTGCAATGCTGACTCTACTTCAATGCAAAGAGTCAAATGAGTGGCAAGGCTACACAAAACAGAT
TCAACCAATTGATTTGCCTTTATGGGCTAAGAAAGAATTTCTAGGAGAATAA

Upstream 100 bases:

>100_bases
TGATGAACATGCAAATCATGTAATTCATAAGCTGAAAAGAACCGCCGAAGAACTCACTGAAATTCTAGAAGGCTTCTTAG
GAAAGAATTAGGAAAAGATT

Downstream 100 bases:

>100_bases
CAATGAATATGCTTGCAACATTAAATCAAGGCATTGTTCCTCAAGCTGAAACAGCAGCAAATGTACTTGCAGCACAAGCA
AAGGCTCAAGTTGAAGCGCG

Product: hypothetical protein

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNAPVLVHNMSNAAYHAHSAVSSSQLKTILRSPAHFFAEHMSGKEHKQTTAMALGTAVHVLFLEPEVFNDEVAIEPIVNK
RTNVGKEAIAKFLQDNASKTIITEEQYQAAAKAAEAMKRHPMYNMILSGGIREASIFFDDEETGLECRIRPDWHVAPETS
EYFPNGLIVDIKKTTDARANAFSRSCQNYDYSLSAAMYINGYKAYYGDEYNPSFLFFAVEEDDPHESIIYYASDEMLFIG
EQKRRSAMLTLLQCKESNEWQGYTKQIQPIDLPLWAKKEFLGE

Sequences:

>Translated_283_residues
MNAPVLVHNMSNAAYHAHSAVSSSQLKTILRSPAHFFAEHMSGKEHKQTTAMALGTAVHVLFLEPEVFNDEVAIEPIVNK
RTNVGKEAIAKFLQDNASKTIITEEQYQAAAKAAEAMKRHPMYNMILSGGIREASIFFDDEETGLECRIRPDWHVAPETS
EYFPNGLIVDIKKTTDARANAFSRSCQNYDYSLSAAMYINGYKAYYGDEYNPSFLFFAVEEDDPHESIIYYASDEMLFIG
EQKRRSAMLTLLQCKESNEWQGYTKQIQPIDLPLWAKKEFLGE
>Mature_283_residues
MNAPVLVHNMSNAAYHAHSAVSSSQLKTILRSPAHFFAEHMSGKEHKQTTAMALGTAVHVLFLEPEVFNDEVAIEPIVNK
RTNVGKEAIAKFLQDNASKTIITEEQYQAAAKAAEAMKRHPMYNMILSGGIREASIFFDDEETGLECRIRPDWHVAPETS
EYFPNGLIVDIKKTTDARANAFSRSCQNYDYSLSAAMYINGYKAYYGDEYNPSFLFFAVEEDDPHESIIYYASDEMLFIG
EQKRRSAMLTLLQCKESNEWQGYTKQIQPIDLPLWAKKEFLGE

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=281, Percent_Identity=32.3843416370107, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 32072; Mature: 32072

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVLVHNMSNAAYHAHSAVSSSQLKTILRSPAHFFAEHMSGKEHKQTTAMALGTAVHV
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHEEEE
LFLEPEVFNDEVAIEPIVNKRTNVGKEAIAKFLQDNASKTIITEEQYQAAAKAAEAMKRH
EEECCCCCCCCEEECCHHCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHC
PMYNMILSGGIREASIFFDDEETGLECRIRPDWHVAPETSEYFPNGLIVDIKKTTDARAN
CHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCHHHH
AFSRSCQNYDYSLSAAMYINGYKAYYGDEYNPSFLFFAVEEDDPHESIIYYASDEMLFIG
HHHHHHCCCCCEEEEEEEEECEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCEEEEC
EQKRRSAMLTLLQCKESNEWQGYTKQIQPIDLPLWAKKEFLGE
CHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MNAPVLVHNMSNAAYHAHSAVSSSQLKTILRSPAHFFAEHMSGKEHKQTTAMALGTAVHV
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHEEEE
LFLEPEVFNDEVAIEPIVNKRTNVGKEAIAKFLQDNASKTIITEEQYQAAAKAAEAMKRH
EEECCCCCCCCEEECCHHCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHC
PMYNMILSGGIREASIFFDDEETGLECRIRPDWHVAPETSEYFPNGLIVDIKKTTDARAN
CHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCHHHH
AFSRSCQNYDYSLSAAMYINGYKAYYGDEYNPSFLFFAVEEDDPHESIIYYASDEMLFIG
HHHHHHCCCCCEEEEEEEEECEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCEEEEC
EQKRRSAMLTLLQCKESNEWQGYTKQIQPIDLPLWAKKEFLGE
CHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]