Click here to switch to the map view.

The map label for this gene is mnmC [H]

Identifier: 213157935

GI number: 213157935

Start: 3535444

End: 3537315

Strand: Direct

Name: mnmC [H]

Synonym: AB57_3428

Alternate gene names: 213157935

Gene position: 3535444-3537315 (Clockwise)

Preceding gene: 213157933

Following gene: 213157936

Centisome position: 87.28

GC content: 39.8

Gene sequence:

>1872_bases
ATGAACAAATCAAACCGTATACAAACTGCTGAGCTGGATTGGGAATCTATTGATGGTATTGAAGTTCCCATTTCCAAACA
ATTTGGTGATGTCTATTTTTCTAAAGATAATGGTCTGCTTGAAACACGGCATGTATTTTTAAATGGCAATGACCTATCAG
AACGATTAGCCAATTTACAAGATTTTGAATATTTCAGTGTAGGAGAAACCGGGTTCGGCACTGGTTTAAATATTCTTGCG
CTCTGGCAACTATGGCAACAAGTTCGTCCAAATAACCACAGTCATTTACATGCAATTAGCGTCGAAAAGTTTCCTTTAAG
CAAAGCTGATCTCATTCGTGCTTTAAATGTATGGGATGAACTTAAACCGCTTTCCAAACAACTTATTGAGCAATATCCCT
TACCTCTTGCAGGCTGCCATCGTTTAAGTTTTCCAGAAGAGCGCTTCAGCATCGACCTATGGTTAGGAGATGCACAAGAT
ATTTTTCCAAGTATGGTAAAAACGAAGGCAGTGAATGCTTGGTTTCTGGATGGTTTTGCGCCTTCTTGTAATCCGGACAT
GTGGGAACAAAATGTTTTAAATAATATTGTTCGGCTTTCCAACTATGGCACTACCTTTGCCTCATTTAGTGTAGCTGGTG
TCCTCAAACGTGGACTAAAGGCTCATGGAATCGATATTTCTCGTCCTCGCGGTTTTGGACATAAAAGGGAAATGCTTAAA
GCCATTTGGAAAGCACCTGTAAGTGAAGAAATTTTATTTGAACCAGTTAGCGATACTTTCCATTTTACTCAGCAGCGTAT
CGCCATAATTGGCGCTGGGATTGCAGGTTTAAGCACAGCTTGGGCATTTGCTCAACGTGGGCATCAGGTCACTCTATTTG
AGCGTACAGCGCCGTTATCGGGTGCTTCTGGCAATCCGTTGGCATTGCTTAATCCGAAACTATGCCCAATTGAACAAAGC
CATGAGCATCTCATGACTTTAAGCTGGCAACATGCATTAAATTTCTATAAAAATTTTCAAGCCTTTCGCCCGATTCAAAT
ACAACAAATGGCTTTAAAAAATGCACAAGATCTTCTCGATCTTACTAATCAATATCCTGCTGATATTGTCACACAGCAAA
CAAGCTCACTTGAATCCGACTATCCACACATTATATTGACCGAAGCTGGAGCAGTTTCTCCGCACCAATTACGTGATGAA
ATTTTGCAACATCCAGGTATTGAACTGAAAATTGCCAATATTACGACACTTGTTTCTTTTGAAAATAAAGTTCAATTAAA
AAGTGGTAATGAAACTACTTTAGAGGCTGATCATGTCGTGGTGTGCTGCGCCAGAGAAAGTGCTGCTTTATTTGAAAATT
ATCCGCTATTAAAACCTATACGTGGACAGGTGAGCTGGGTTGATAATTCTTTTGCAACCTTACCTTTACACGAAGCCTAT
AGCTATGGCGGTTATTGTATGCAACTGAATACGTCTGAACTTATTCTAGGGGCTTCTTTCTACCCAAACCGAGACGATCA
AGAAGTACTGCTTGATGACCATGTGCATAACTTTGAATTAATCCACAGCGTTTTTCCTCATTATGCAAAACAATTACCGC
CAGTCGAACAATGGCAAGGACGAGCATCAGTTCGTGCTCAAAGTCCAGATTATTTTCCAGTTGTGGGAAAAATGCAGAAT
GAAAGCCGTATTTCTACTTTTGCAGGTTTAGGCTCAAAGGGCTTTTTATTTGCGCCGCTTTGTAGTGAAGTTTTAGTGGC
CCAAATTTTAGGTGAAGCTTATCCTGTTCCCAAAAGCCTATTACAGAAATTAGATGCCCAACGTTTTCAAAAGAAAGTTA
AACCTAAGAAACCTTATTTTAAATCTCAATAG

Upstream 100 bases:

>100_bases
TAACTCTTGCAGGTGGTGTACTGCTTGGCTTCTTTATTGCGAGTTATGTTTACACCAAACGTCGACGTCAGTGGTAATTC
TGAGCGACTTATTAAACCGA

Downstream 100 bases:

>100_bases
TGCACTTGAGAAAATTTGGTCCCAATAAAAAAAGCACCCGAAGGTGCTTTTTTTATTGCGCCCTGCTCTAGAGGTAAACC
TGCGTCACGAGCTGCACGCG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MNKSNRIQTAELDWESIDGIEVPISKQFGDVYFSKDNGLLETRHVFLNGNDLSERLANLQDFEYFSVGETGFGTGLNILA
LWQLWQQVRPNNHSHLHAISVEKFPLSKADLIRALNVWDELKPLSKQLIEQYPLPLAGCHRLSFPEERFSIDLWLGDAQD
IFPSMVKTKAVNAWFLDGFAPSCNPDMWEQNVLNNIVRLSNYGTTFASFSVAGVLKRGLKAHGIDISRPRGFGHKREMLK
AIWKAPVSEEILFEPVSDTFHFTQQRIAIIGAGIAGLSTAWAFAQRGHQVTLFERTAPLSGASGNPLALLNPKLCPIEQS
HEHLMTLSWQHALNFYKNFQAFRPIQIQQMALKNAQDLLDLTNQYPADIVTQQTSSLESDYPHIILTEAGAVSPHQLRDE
ILQHPGIELKIANITTLVSFENKVQLKSGNETTLEADHVVVCCARESAALFENYPLLKPIRGQVSWVDNSFATLPLHEAY
SYGGYCMQLNTSELILGASFYPNRDDQEVLLDDHVHNFELIHSVFPHYAKQLPPVEQWQGRASVRAQSPDYFPVVGKMQN
ESRISTFAGLGSKGFLFAPLCSEVLVAQILGEAYPVPKSLLQKLDAQRFQKKVKPKKPYFKSQ

Sequences:

>Translated_623_residues
MNKSNRIQTAELDWESIDGIEVPISKQFGDVYFSKDNGLLETRHVFLNGNDLSERLANLQDFEYFSVGETGFGTGLNILA
LWQLWQQVRPNNHSHLHAISVEKFPLSKADLIRALNVWDELKPLSKQLIEQYPLPLAGCHRLSFPEERFSIDLWLGDAQD
IFPSMVKTKAVNAWFLDGFAPSCNPDMWEQNVLNNIVRLSNYGTTFASFSVAGVLKRGLKAHGIDISRPRGFGHKREMLK
AIWKAPVSEEILFEPVSDTFHFTQQRIAIIGAGIAGLSTAWAFAQRGHQVTLFERTAPLSGASGNPLALLNPKLCPIEQS
HEHLMTLSWQHALNFYKNFQAFRPIQIQQMALKNAQDLLDLTNQYPADIVTQQTSSLESDYPHIILTEAGAVSPHQLRDE
ILQHPGIELKIANITTLVSFENKVQLKSGNETTLEADHVVVCCARESAALFENYPLLKPIRGQVSWVDNSFATLPLHEAY
SYGGYCMQLNTSELILGASFYPNRDDQEVLLDDHVHNFELIHSVFPHYAKQLPPVEQWQGRASVRAQSPDYFPVVGKMQN
ESRISTFAGLGSKGFLFAPLCSEVLVAQILGEAYPVPKSLLQKLDAQRFQKKVKPKKPYFKSQ
>Mature_623_residues
MNKSNRIQTAELDWESIDGIEVPISKQFGDVYFSKDNGLLETRHVFLNGNDLSERLANLQDFEYFSVGETGFGTGLNILA
LWQLWQQVRPNNHSHLHAISVEKFPLSKADLIRALNVWDELKPLSKQLIEQYPLPLAGCHRLSFPEERFSIDLWLGDAQD
IFPSMVKTKAVNAWFLDGFAPSCNPDMWEQNVLNNIVRLSNYGTTFASFSVAGVLKRGLKAHGIDISRPRGFGHKREMLK
AIWKAPVSEEILFEPVSDTFHFTQQRIAIIGAGIAGLSTAWAFAQRGHQVTLFERTAPLSGASGNPLALLNPKLCPIEQS
HEHLMTLSWQHALNFYKNFQAFRPIQIQQMALKNAQDLLDLTNQYPADIVTQQTSSLESDYPHIILTEAGAVSPHQLRDE
ILQHPGIELKIANITTLVSFENKVQLKSGNETTLEADHVVVCCARESAALFENYPLLKPIRGQVSWVDNSFATLPLHEAY
SYGGYCMQLNTSELILGASFYPNRDDQEVLLDDHVHNFELIHSVFPHYAKQLPPVEQWQGRASVRAQSPDYFPVVGKMQN
ESRISTFAGLGSKGFLFAPLCSEVLVAQILGEAYPVPKSLLQKLDAQRFQKKVKPKKPYFKSQ

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=667, Percent_Identity=33.1334332833583, Blast_Score=298, Evalue=6e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR016040
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 70137; Mature: 70137

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSNRIQTAELDWESIDGIEVPISKQFGDVYFSKDNGLLETRHVFLNGNDLSERLANLQ
CCCCCCCEEEECCHHHCCCEECCCCCCCCCEEEECCCCEEEEEEEEECCCHHHHHHCCCC
DFEYFSVGETGFGTGLNILALWQLWQQVRPNNHSHLHAISVEKFPLSKADLIRALNVWDE
CCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
LKPLSKQLIEQYPLPLAGCHRLSFPEERFSIDLWLGDAQDIFPSMVKTKAVNAWFLDGFA
HHHHHHHHHHHCCCCHHCCCCCCCCHHHEEEEEEECCHHHHHHHHHHHHHCCEEEECCCC
PSCNPDMWEQNVLNNIVRLSNYGTTFASFSVAGVLKRGLKAHGIDISRPRGFGHKREMLK
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHH
AIWKAPVSEEILFEPVSDTFHFTQQRIAIIGAGIAGLSTAWAFAQRGHQVTLFERTAPLS
HHHCCCCCCHHHCCCCHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCC
GASGNPLALLNPKLCPIEQSHEHLMTLSWQHALNFYKNFQAFRPIQIQQMALKNAQDLLD
CCCCCEEEEECCCCCCCCCCCHHHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LTNQYPADIVTQQTSSLESDYPHIILTEAGAVSPHQLRDEILQHPGIELKIANITTLVSF
HHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECEEEEEEE
ENKVQLKSGNETTLEADHVVVCCARESAALFENYPLLKPIRGQVSWVDNSFATLPLHEAY
CCEEEEECCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCCCCEEEECCCEEEEEHHHHH
SYGGYCMQLNTSELILGASFYPNRDDQEVLLDDHVHNFELIHSVFPHYAKQLPPVEQWQG
CCCCEEEEECCCEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCC
RASVRAQSPDYFPVVGKMQNESRISTFAGLGSKGFLFAPLCSEVLVAQILGEAYPVPKSL
CCEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
LQKLDAQRFQKKVKPKKPYFKSQ
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNKSNRIQTAELDWESIDGIEVPISKQFGDVYFSKDNGLLETRHVFLNGNDLSERLANLQ
CCCCCCCEEEECCHHHCCCEECCCCCCCCCEEEECCCCEEEEEEEEECCCHHHHHHCCCC
DFEYFSVGETGFGTGLNILALWQLWQQVRPNNHSHLHAISVEKFPLSKADLIRALNVWDE
CCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
LKPLSKQLIEQYPLPLAGCHRLSFPEERFSIDLWLGDAQDIFPSMVKTKAVNAWFLDGFA
HHHHHHHHHHHCCCCHHCCCCCCCCHHHEEEEEEECCHHHHHHHHHHHHHCCEEEECCCC
PSCNPDMWEQNVLNNIVRLSNYGTTFASFSVAGVLKRGLKAHGIDISRPRGFGHKREMLK
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHH
AIWKAPVSEEILFEPVSDTFHFTQQRIAIIGAGIAGLSTAWAFAQRGHQVTLFERTAPLS
HHHCCCCCCHHHCCCCHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCC
GASGNPLALLNPKLCPIEQSHEHLMTLSWQHALNFYKNFQAFRPIQIQQMALKNAQDLLD
CCCCCEEEEECCCCCCCCCCCHHHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LTNQYPADIVTQQTSSLESDYPHIILTEAGAVSPHQLRDEILQHPGIELKIANITTLVSF
HHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECEEEEEEE
ENKVQLKSGNETTLEADHVVVCCARESAALFENYPLLKPIRGQVSWVDNSFATLPLHEAY
CCEEEEECCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCCCCEEEECCCEEEEEHHHHH
SYGGYCMQLNTSELILGASFYPNRDDQEVLLDDHVHNFELIHSVFPHYAKQLPPVEQWQG
CCCCEEEEECCCEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCC
RASVRAQSPDYFPVVGKMQNESRISTFAGLGSKGFLFAPLCSEVLVAQILGEAYPVPKSL
CCEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
LQKLDAQRFQKKVKPKKPYFKSQ
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA