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The map label for this gene is queA [H]

Identifier: 213157920

GI number: 213157920

Start: 3522429

End: 3523466

Strand: Direct

Name: queA [H]

Synonym: AB57_3412

Alternate gene names: 213157920

Gene position: 3522429-3523466 (Clockwise)

Preceding gene: 213157912

Following gene: 213157921

Centisome position: 86.96

GC content: 41.81

Gene sequence:

>1038_bases
ATGCAACTGTCTGACTTTTCCTTTGAACTACCCGATGAACTTATTGCCCGTTACCCACTCGAAACACGTAGTGCTTCGCG
TTTATTGCATTTAGATGCTAAGGGCCAATATCACGATCATATGTTCACAGATATTATTGATCTGTTCGAAGAAGGCGATT
TATTGGTACTCAACGATACCAAAGTCATGAAGGCTCGACTGAAAGGAAAACGTGCCACAGGTGGGGCGATTGAAATTTTG
GTTGAGCGTATGCTGAACCACACCACAGCGTATTGCCATATTAAAGCGAGCAATTCACCTAAAGCAGGGGCTGAGCTTTT
TGTTGGTGCGGACAATATTCCCGTGATTGTGCGTGGCCGTCACGAAAACTTATTTGTCGTTGAGTTTTCACAACCAATTT
TGCCAGTACTTGAGCAATATGGTCAGTTACCTATTCCGCCTTACTTCAATCGTGAAGCAGAAGAAATTGACACTGAACGC
TATCAAACGGTTTTCCATAATCCAGAAAAAATTGCCAGTGTGGCTGCCCCAACAGCAAGCTTGCATTTTGACGAAGAGTT
GTTAGCAGAGTTAGACCAAAAAGGTGTTAAGAAAACGTTTGTGACTCTTCACGTCGGTGCAGGTACTTTTATGCCTGTAC
GCACTGATGACATTACAAATCATGTTATGCACAGTGAATGGTGTGATGTTCCTCAAGAAACTATTGATTTAATTTTGGCA
ACTAAAGCACGCGGTAATAAAGTGATTGCTGTCGGTACAACTGCAACACGTGCTTTAGAAAGTGCAGCTCAAGCGCATGG
CGGAAAAATCGCAGCATGGACTGGTGATACGCAAATCTTTATTTATCCGGGTTATGAGTTCTGCATCGTAGACCGTTTAA
TTACTAACTTCCATTTACCTGAGTCTACCCTGCTCATGTTAGTTTCAGCATTGTCAAATAGAGAAAATATTTTGGCTGCT
TATGAACATGCCGTTAAAGATCGCTATCGCTTCTTTAGTTATGGCGATGCAATGCTGATTGATAAATTAGAAGTTTAA

Upstream 100 bases:

>100_bases
AGATCATTTAAACATTTTTCATGATTTAGGCCAGAAGCACAGCCATTAAAAAGCGAAAAAGTTTATACTAGGCGCAATTT
TTGCGGTGTTTTTCCTGAAT

Downstream 100 bases:

>100_bases
AGATTAAAGCTGGGTTAAGTCTTATTAGTTAATTTGAGGTTTAACCCAGCATCTATAATTAAAATAAAAATAGTCTTAGT
ATTTCTATTTATCTCTTTCA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MQLSDFSFELPDELIARYPLETRSASRLLHLDAKGQYHDHMFTDIIDLFEEGDLLVLNDTKVMKARLKGKRATGGAIEIL
VERMLNHTTAYCHIKASNSPKAGAELFVGADNIPVIVRGRHENLFVVEFSQPILPVLEQYGQLPIPPYFNREAEEIDTER
YQTVFHNPEKIASVAAPTASLHFDEELLAELDQKGVKKTFVTLHVGAGTFMPVRTDDITNHVMHSEWCDVPQETIDLILA
TKARGNKVIAVGTTATRALESAAQAHGGKIAAWTGDTQIFIYPGYEFCIVDRLITNFHLPESTLLMLVSALSNRENILAA
YEHAVKDRYRFFSYGDAMLIDKLEV

Sequences:

>Translated_345_residues
MQLSDFSFELPDELIARYPLETRSASRLLHLDAKGQYHDHMFTDIIDLFEEGDLLVLNDTKVMKARLKGKRATGGAIEIL
VERMLNHTTAYCHIKASNSPKAGAELFVGADNIPVIVRGRHENLFVVEFSQPILPVLEQYGQLPIPPYFNREAEEIDTER
YQTVFHNPEKIASVAAPTASLHFDEELLAELDQKGVKKTFVTLHVGAGTFMPVRTDDITNHVMHSEWCDVPQETIDLILA
TKARGNKVIAVGTTATRALESAAQAHGGKIAAWTGDTQIFIYPGYEFCIVDRLITNFHLPESTLLMLVSALSNRENILAA
YEHAVKDRYRFFSYGDAMLIDKLEV
>Mature_345_residues
MQLSDFSFELPDELIARYPLETRSASRLLHLDAKGQYHDHMFTDIIDLFEEGDLLVLNDTKVMKARLKGKRATGGAIEIL
VERMLNHTTAYCHIKASNSPKAGAELFVGADNIPVIVRGRHENLFVVEFSQPILPVLEQYGQLPIPPYFNREAEEIDTER
YQTVFHNPEKIASVAAPTASLHFDEELLAELDQKGVKKTFVTLHVGAGTFMPVRTDDITNHVMHSEWCDVPQETIDLILA
TKARGNKVIAVGTTATRALESAAQAHGGKIAAWTGDTQIFIYPGYEFCIVDRLITNFHLPESTLLMLVSALSNRENILAA
YEHAVKDRYRFFSYGDAMLIDKLEV

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=55.6521739130435, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38701; Mature: 38701

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSDFSFELPDELIARYPLETRSASRLLHLDAKGQYHDHMFTDIIDLFEEGDLLVLNDT
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECH
KVMKARLKGKRATGGAIEILVERMLNHTTAYCHIKASNSPKAGAELFVGADNIPVIVRGR
HHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEEC
HENLFVVEFSQPILPVLEQYGQLPIPPYFNREAEEIDTERYQTVFHNPEKIASVAAPTAS
CCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHH
LHFDEELLAELDQKGVKKTFVTLHVGAGTFMPVRTDDITNHVMHSEWCDVPQETIDLILA
CCCCHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCHHHHHEEEE
TKARGNKVIAVGTTATRALESAAQAHGGKIAAWTGDTQIFIYPGYEFCIVDRLITNFHLP
ECCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCHHHHHHHHHHHCCCC
ESTLLMLVSALSNRENILAAYEHAVKDRYRFFSYGDAMLIDKLEV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MQLSDFSFELPDELIARYPLETRSASRLLHLDAKGQYHDHMFTDIIDLFEEGDLLVLNDT
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECH
KVMKARLKGKRATGGAIEILVERMLNHTTAYCHIKASNSPKAGAELFVGADNIPVIVRGR
HHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEEC
HENLFVVEFSQPILPVLEQYGQLPIPPYFNREAEEIDTERYQTVFHNPEKIASVAAPTAS
CCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHH
LHFDEELLAELDQKGVKKTFVTLHVGAGTFMPVRTDDITNHVMHSEWCDVPQETIDLILA
CCCCHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCHHHHHEEEE
TKARGNKVIAVGTTATRALESAAQAHGGKIAAWTGDTQIFIYPGYEFCIVDRLITNFHLP
ECCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCHHHHHHHHHHHCCCC
ESTLLMLVSALSNRENILAAYEHAVKDRYRFFSYGDAMLIDKLEV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA