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The map label for this gene is thlA [H]

Identifier: 213157859

GI number: 213157859

Start: 3449829

End: 3451004

Strand: Direct

Name: thlA [H]

Synonym: AB57_3346

Alternate gene names: 213157859

Gene position: 3449829-3451004 (Clockwise)

Preceding gene: 213157858

Following gene: 213157860

Centisome position: 85.17

GC content: 42.77

Gene sequence:

>1176_bases
ATGACTGACATCGTAATTGTTAATGGTGCTCGTACTGCTATGGGTGGTTTTCAAGGATCACTATCAGGACTAACTGCTCC
TGAATTAGGAGCGGTAACAATTAAAGAAGCAATCGCACGAGCAGGTTTACAGCCTACAGATGTCGAAGAAGTCATTATGG
GTTGCGTACTACCAGCGGGTTTAAAGCAAGGCCCTGCTCGTCAAGCCATGCGTAAAGCAGGTTTACCTGATTCAACAGGA
GCTGTGACAATTAATAAGCTTTGTGGTTCAGGTATGAAGGCGGTAATGCAAGCGGCTGATATGATTAAAGCAGGAAGTGC
TGAAATTGTAGTTGCCGGTGGTATGGAGTCGATGACCAATGCGCCTTATGTCTTACCCAAAGCACGTGCAGGATACCGTA
TGGGCCACGGTGAAATTAAAGACCATATGTTCTTTGATGGTTTAGAAGATGCTGAAACTGGTCGTTTAATGGGTTCATTT
GCTCAAGATATGGCAAATACACGTGGTTATACTCGTGAGCAAATGGATGACTTTGCAATTCGTTCTTTAAAACGTGCTCA
AACTGCGATTACTGAAGGCTATTTCAAAGATGAAATTGTACCTGTTACTGTTTCTACCCGTAAAGGTGATGTCATTGTCG
ATCAGGATGAACAACCCTTAAATGCCAAGATTGATAAAATCCCATCCCTTAAACCAGCTTTTGCAAAAGACGGTACAATT
ACTGCTGCAAACGCGAGTTCAATTTCTGATGGTGCTTCTGCACTAGTTTTAACATCGAGTGAAGTTGCAGCACAGCGCGG
CTTACAACCACTTGCAAAAATCATAGCCACTGCGTCAAATTCTCAACATCCTTCAGAATTTACAATTGCTCCTGTTGGCG
CTATTGAAAAAGTACTTAAAAAAGCAGGTTGGAATGCTCAGGACGTTGACCTTTGGGAAATTAATGAAGCTTTTGCTATG
GTAACGATGTGTCCAATTGATGATTTCAAACTTGATCCAGAAAAAGTCAATATTCATGGTGGAGCATGTGCACTTGGTCA
TCCTGTCGGATCGACAGGTTCTCGTATTATTCTTACGCTTATTCATGCGCTTAAACGTACTGGTGGTAAAAAGGGTGTCG
CTGCTCTTTGCATCGGCGGTGGAGAAGCAACAGCTGTTGCCATCGAAATTCTTTAA

Upstream 100 bases:

>100_bases
AGTCTAATGCAGAAGCTAACCCGTTCTTCATTTTGCAATATACTGCTTAGGTTTTAAACATTTCTTATTTTGAGATTAAG
AACAATCGGAGATTACAACA

Downstream 100 bases:

>100_bases
CTAAAGCCTTTTTTAAAATCCCACTTCGGTGGGATTTTTTATGAAAAAACTACAAAAAATGACCCACAAAATCTCTTTTC
CGTTTTAACTTATTCTTCTA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTDIVIVNGARTAMGGFQGSLSGLTAPELGAVTIKEAIARAGLQPTDVEEVIMGCVLPAGLKQGPARQAMRKAGLPDSTG
AVTINKLCGSGMKAVMQAADMIKAGSAEIVVAGGMESMTNAPYVLPKARAGYRMGHGEIKDHMFFDGLEDAETGRLMGSF
AQDMANTRGYTREQMDDFAIRSLKRAQTAITEGYFKDEIVPVTVSTRKGDVIVDQDEQPLNAKIDKIPSLKPAFAKDGTI
TAANASSISDGASALVLTSSEVAAQRGLQPLAKIIATASNSQHPSEFTIAPVGAIEKVLKKAGWNAQDVDLWEINEAFAM
VTMCPIDDFKLDPEKVNIHGGACALGHPVGSTGSRIILTLIHALKRTGGKKGVAALCIGGGEATAVAIEIL

Sequences:

>Translated_391_residues
MTDIVIVNGARTAMGGFQGSLSGLTAPELGAVTIKEAIARAGLQPTDVEEVIMGCVLPAGLKQGPARQAMRKAGLPDSTG
AVTINKLCGSGMKAVMQAADMIKAGSAEIVVAGGMESMTNAPYVLPKARAGYRMGHGEIKDHMFFDGLEDAETGRLMGSF
AQDMANTRGYTREQMDDFAIRSLKRAQTAITEGYFKDEIVPVTVSTRKGDVIVDQDEQPLNAKIDKIPSLKPAFAKDGTI
TAANASSISDGASALVLTSSEVAAQRGLQPLAKIIATASNSQHPSEFTIAPVGAIEKVLKKAGWNAQDVDLWEINEAFAM
VTMCPIDDFKLDPEKVNIHGGACALGHPVGSTGSRIILTLIHALKRTGGKKGVAALCIGGGEATAVAIEIL
>Mature_390_residues
TDIVIVNGARTAMGGFQGSLSGLTAPELGAVTIKEAIARAGLQPTDVEEVIMGCVLPAGLKQGPARQAMRKAGLPDSTGA
VTINKLCGSGMKAVMQAADMIKAGSAEIVVAGGMESMTNAPYVLPKARAGYRMGHGEIKDHMFFDGLEDAETGRLMGSFA
QDMANTRGYTREQMDDFAIRSLKRAQTAITEGYFKDEIVPVTVSTRKGDVIVDQDEQPLNAKIDKIPSLKPAFAKDGTIT
AANASSISDGASALVLTSSEVAAQRGLQPLAKIIATASNSQHPSEFTIAPVGAIEKVLKKAGWNAQDVDLWEINEAFAMV
TMCPIDDFKLDPEKVNIHGGACALGHPVGSTGSRIILTLIHALKRTGGKKGVAALCIGGGEATAVAIEIL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=46.7866323907455, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=44.529262086514, Blast_Score=328, Evalue=7e-90,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=38.0462724935733, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=36, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=30.2107728337237, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=49.438202247191, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=48.3460559796438, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI87082165, Length=390, Percent_Identity=43.8461538461538, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=37.5, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=36.3861386138614, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788683, Length=405, Percent_Identity=28.641975308642, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI25147385, Length=386, Percent_Identity=42.4870466321244, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17535921, Length=395, Percent_Identity=44.3037974683544, Blast_Score=317, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI133906874, Length=388, Percent_Identity=38.659793814433, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17535917, Length=397, Percent_Identity=35.264483627204, Blast_Score=214, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=30.188679245283, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=393, Percent_Identity=47.3282442748092, Blast_Score=338, Evalue=9e-94,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=35.8778625954198, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=46.1734693877551, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24640423, Length=393, Percent_Identity=45.8015267175573, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=37.7551020408163, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17137578, Length=422, Percent_Identity=31.2796208530806, Blast_Score=175, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40819; Mature: 40688

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIVIVNGARTAMGGFQGSLSGLTAPELGAVTIKEAIARAGLQPTDVEEVIMGCVLPAG
CCCEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
LKQGPARQAMRKAGLPDSTGAVTINKLCGSGMKAVMQAADMIKAGSAEIVVAGGMESMTN
CCCCHHHHHHHHCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHHCC
APYVLPKARAGYRMGHGEIKDHMFFDGLEDAETGRLMGSFAQDMANTRGYTREQMDDFAI
CCEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RSLKRAQTAITEGYFKDEIVPVTVSTRKGDVIVDQDEQPLNAKIDKIPSLKPAFAKDGTI
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCCCHHHCCCCCCCCCCCCCEE
TAANASSISDGASALVLTSSEVAAQRGLQPLAKIIATASNSQHPSEFTIAPVGAIEKVLK
EECCCCCCCCCCCEEEEECHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHH
KAGWNAQDVDLWEINEAFAMVTMCPIDDFKLDPEKVNIHGGACALGHPVGSTGSRIILTL
HCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEEECCCEEEECCCCCCCCHHHHHHH
IHALKRTGGKKGVAALCIGGGEATAVAIEIL
HHHHHHCCCCCCEEEEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TDIVIVNGARTAMGGFQGSLSGLTAPELGAVTIKEAIARAGLQPTDVEEVIMGCVLPAG
CCEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
LKQGPARQAMRKAGLPDSTGAVTINKLCGSGMKAVMQAADMIKAGSAEIVVAGGMESMTN
CCCCHHHHHHHHCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHHCC
APYVLPKARAGYRMGHGEIKDHMFFDGLEDAETGRLMGSFAQDMANTRGYTREQMDDFAI
CCEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RSLKRAQTAITEGYFKDEIVPVTVSTRKGDVIVDQDEQPLNAKIDKIPSLKPAFAKDGTI
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCCCHHHCCCCCCCCCCCCCEE
TAANASSISDGASALVLTSSEVAAQRGLQPLAKIIATASNSQHPSEFTIAPVGAIEKVLK
EECCCCCCCCCCCEEEEECHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHH
KAGWNAQDVDLWEINEAFAMVTMCPIDDFKLDPEKVNIHGGACALGHPVGSTGSRIILTL
HCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEEECCCEEEECCCCCCCCHHHHHHH
IHALKRTGGKKGVAALCIGGGEATAVAIEIL
HHHHHHCCCCCCEEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]