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The map label for this gene is pilJ [H]

Identifier: 213157828

GI number: 213157828

Start: 3417347

End: 3419425

Strand: Reverse

Name: pilJ [H]

Synonym: AB57_3312

Alternate gene names: 213157828

Gene position: 3419425-3417347 (Counterclockwise)

Preceding gene: 213157829

Following gene: 213157827

Centisome position: 84.42

GC content: 40.36

Gene sequence:

>2079_bases
ATGGGCTTTAAACTTAAAAAGAAAAGTGGCAATCGCGGTGCTACCGGAAAATCGAGTGTCAATTTTATTGCAAAAATCCA
GTCAAAAATTGAGCAGTTTGCCAGTTTATTTGGGGAAAGTGAAAAGTCCAAGCCTATCCTTTATGCAGCGCTGTTATGTT
TAGTATGTGCGTTAATTTCATTAGCTTATCTATTTTATAATGTACCTCGTCATAACCAGCTAATTCGAAGTCTTGGTGAG
TTACGTCTGTTATCTCAAACCATTTCTAAACAAGCAACTGAAGCAACAGAGTCGGGTTCTCAAGAGGCAATCACAAAGCT
ACAGCAATCTAAAAAAGACTTTGATGAGAACTTACTTGAAATTGAAAATATTCACGGTAAGTCGACCGATGAATATCAAA
AAGTTGAGAAAATATGGGGTGAGGTTTCAAAAAATATTGATTTGATTTCATCACATCAACGAGTACTTAACCAGTTATAT
GATACCAACATCTCGATTAGTGAAACGGTTCCAGAGATTCAGGCAGAATATAACTTGATGGTCGACCAAATGGCTCGCCA
AGGTTTGCCAAGTAGTCAGGTAATTATTGCTAAAAACCAGGTATTTATTGCAGAGCGTATTTTACGTTCGATTAACTCGG
TGTTAAGTGGTACGGACGGAAATGTATCAACTAGTGACTTTAGTGTCGATATTGAGACTTTTGGTACCTATTTAAATGCT
GAACTGAATGGTAATGCCGAGTTAGGTGTTGATCGTATTGCTGATCCGGCTTTACGTGAATCTTTAGAAAGTATTAAGTC
TGAATATGACAAAGTCTTAAAATCTGCTGCTGCAACGGTATTAAAAAATGGTAGCCAGATTGTCAATGTTCGTCAGGCTT
CTTCTCAGATTTTCTCTAAATCGGATGTAATGTTGGAAAGTCTAGGACATTTATCTGATGTTGCAAGAAAGAACTGGCTC
ACCTTATTTTTAGGTGCTGTTCTTATTCTTTCATTAGGCGGACTAATTTTCTCTGTATTCAAGTTAATTACACTACGTAG
TGTAATGGATAAGCAACGTGTTGCTCGATTACAGGAAGAATATGACCGTAACCAGAATGCGATTTTACGTTTACTTGATG
AAATTGCCGATTTGGCGGATGGTGACTTACGTTCATATGCAACTGTATCTGAAGACTTTACGGGTGCCATTGCCGACTCG
ATTAACTTCGCAATTGACCAACTGCGAGATTTGGTTTCACGAATTCATGAAACCTCTCAGGAAGTTGCACGCTACACACA
AGATACGCAAAGTATTACTAACCAGTTAGCAGAGGCATCAGAACATCAGGCGCAAGAAATTGCAGGTGCTTCTACTGCCA
TGAATGAAATGGCACAGTCAATTGACCAAGTATCTGCCAACGCTTCTGAATCTGCGGAAGTAGCACAGCGTTCAGTACAA
ATTGCATCAAATGGTGCACAAGTGGTAAATCGTTCAATTGAAGGTATGGATACTATTCGTGAGCAGATTCAGGAAACTTC
TAAACGTATTAAGCGCTTAGGTGAGTCTTCTCAAGAAATTGGTAACATCGTATCGCTCATTAACGATATTGCAGACCAAA
CGAACATCCTTGCATTAAATGCAGCAATTCAAGCATCTATGGCAGGTGAGGCTGGTCGTGGTTTCGCCGTAGTTGCCGAC
GAAGTACAACGTCTTGCTGAGCGTTCAGCATCTGCAACCAAACAGATCGAAACACTGGTTAAAACAATTCAGACCGATAC
CAACGAAGCCGTTATTTCAATGGAACAAACAACGACGGAAGTTGTACGTGGTGCGAATTTGGCAAAAGATGCGGGTATTG
CACTGGATGAAATCCAAAAAGTCTCGGGTGACTTGGCAAAATTGATTGCGAGCATTTCCGACGCAGCAAAACTTCAGTCA
GCATCTGCGAGTCACATTGCAACCACCATGACGGTTGTACAAGAAATTACTTCGCAAACAACAACGGCAACGTTTGATAC
TGCTCGCTCCGTATCAGAGTTGGCGAATATGGCAGAGTCATTACGTGAGTCAGTAACGGACTTTAAATTACCAGACTAA

Upstream 100 bases:

>100_bases
CGGTTATTGCAAAACCCACACTACATGAACGCATCGACAAAATTTATAAACTAATTTTTATGCTGTGAATGAACAGCGTA
TTTTTCTGGGGAGAAGCTGT

Downstream 100 bases:

>100_bases
ATTGGGGGATAAGAATAGCTATTCTCATATCGCTATTCTTTTAAAAGTATGAGCAGCGCCAGACAAATATTGGGGAGATA
GTTATTTTTCTGGTGTTGAC

Product: type IV pilus methyl-accepting chemotaxis sensory transducer PilJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MGFKLKKKSGNRGATGKSSVNFIAKIQSKIEQFASLFGESEKSKPILYAALLCLVCALISLAYLFYNVPRHNQLIRSLGE
LRLLSQTISKQATEATESGSQEAITKLQQSKKDFDENLLEIENIHGKSTDEYQKVEKIWGEVSKNIDLISSHQRVLNQLY
DTNISISETVPEIQAEYNLMVDQMARQGLPSSQVIIAKNQVFIAERILRSINSVLSGTDGNVSTSDFSVDIETFGTYLNA
ELNGNAELGVDRIADPALRESLESIKSEYDKVLKSAAATVLKNGSQIVNVRQASSQIFSKSDVMLESLGHLSDVARKNWL
TLFLGAVLILSLGGLIFSVFKLITLRSVMDKQRVARLQEEYDRNQNAILRLLDEIADLADGDLRSYATVSEDFTGAIADS
INFAIDQLRDLVSRIHETSQEVARYTQDTQSITNQLAEASEHQAQEIAGASTAMNEMAQSIDQVSANASESAEVAQRSVQ
IASNGAQVVNRSIEGMDTIREQIQETSKRIKRLGESSQEIGNIVSLINDIADQTNILALNAAIQASMAGEAGRGFAVVAD
EVQRLAERSASATKQIETLVKTIQTDTNEAVISMEQTTTEVVRGANLAKDAGIALDEIQKVSGDLAKLIASISDAAKLQS
ASASHIATTMTVVQEITSQTTTATFDTARSVSELANMAESLRESVTDFKLPD

Sequences:

>Translated_692_residues
MGFKLKKKSGNRGATGKSSVNFIAKIQSKIEQFASLFGESEKSKPILYAALLCLVCALISLAYLFYNVPRHNQLIRSLGE
LRLLSQTISKQATEATESGSQEAITKLQQSKKDFDENLLEIENIHGKSTDEYQKVEKIWGEVSKNIDLISSHQRVLNQLY
DTNISISETVPEIQAEYNLMVDQMARQGLPSSQVIIAKNQVFIAERILRSINSVLSGTDGNVSTSDFSVDIETFGTYLNA
ELNGNAELGVDRIADPALRESLESIKSEYDKVLKSAAATVLKNGSQIVNVRQASSQIFSKSDVMLESLGHLSDVARKNWL
TLFLGAVLILSLGGLIFSVFKLITLRSVMDKQRVARLQEEYDRNQNAILRLLDEIADLADGDLRSYATVSEDFTGAIADS
INFAIDQLRDLVSRIHETSQEVARYTQDTQSITNQLAEASEHQAQEIAGASTAMNEMAQSIDQVSANASESAEVAQRSVQ
IASNGAQVVNRSIEGMDTIREQIQETSKRIKRLGESSQEIGNIVSLINDIADQTNILALNAAIQASMAGEAGRGFAVVAD
EVQRLAERSASATKQIETLVKTIQTDTNEAVISMEQTTTEVVRGANLAKDAGIALDEIQKVSGDLAKLIASISDAAKLQS
ASASHIATTMTVVQEITSQTTTATFDTARSVSELANMAESLRESVTDFKLPD
>Mature_691_residues
GFKLKKKSGNRGATGKSSVNFIAKIQSKIEQFASLFGESEKSKPILYAALLCLVCALISLAYLFYNVPRHNQLIRSLGEL
RLLSQTISKQATEATESGSQEAITKLQQSKKDFDENLLEIENIHGKSTDEYQKVEKIWGEVSKNIDLISSHQRVLNQLYD
TNISISETVPEIQAEYNLMVDQMARQGLPSSQVIIAKNQVFIAERILRSINSVLSGTDGNVSTSDFSVDIETFGTYLNAE
LNGNAELGVDRIADPALRESLESIKSEYDKVLKSAAATVLKNGSQIVNVRQASSQIFSKSDVMLESLGHLSDVARKNWLT
LFLGAVLILSLGGLIFSVFKLITLRSVMDKQRVARLQEEYDRNQNAILRLLDEIADLADGDLRSYATVSEDFTGAIADSI
NFAIDQLRDLVSRIHETSQEVARYTQDTQSITNQLAEASEHQAQEIAGASTAMNEMAQSIDQVSANASESAEVAQRSVQI
ASNGAQVVNRSIEGMDTIREQIQETSKRIKRLGESSQEIGNIVSLINDIADQTNILALNAAIQASMAGEAGRGFAVVADE
VQRLAERSASATKQIETLVKTIQTDTNEAVISMEQTTTEVVRGANLAKDAGIALDEIQKVSGDLAKLIASISDAAKLQSA
SASHIATTMTVVQEITSQTTTATFDTARSVSELANMAESLRESVTDFKLPD

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=316, Percent_Identity=29.746835443038, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788195, Length=324, Percent_Identity=28.3950617283951, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI2367378, Length=322, Percent_Identity=28.2608695652174, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1787690, Length=283, Percent_Identity=30.0353356890459, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1789453, Length=288, Percent_Identity=31.25, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 75362; Mature: 75230

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKLKKKSGNRGATGKSSVNFIAKIQSKIEQFASLFGESEKSKPILYAALLCLVCALIS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LAYLFYNVPRHNQLIRSLGELRLLSQTISKQATEATESGSQEAITKLQQSKKDFDENLLE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IENIHGKSTDEYQKVEKIWGEVSKNIDLISSHQRVLNQLYDTNISISETVPEIQAEYNLM
HHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VDQMARQGLPSSQVIIAKNQVFIAERILRSINSVLSGTDGNVSTSDFSVDIETFGTYLNA
HHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCC
ELNGNAELGVDRIADPALRESLESIKSEYDKVLKSAAATVLKNGSQIVNVRQASSQIFSK
EECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SDVMLESLGHLSDVARKNWLTLFLGAVLILSLGGLIFSVFKLITLRSVMDKQRVARLQEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDRNQNAILRLLDEIADLADGDLRSYATVSEDFTGAIADSINFAIDQLRDLVSRIHETSQ
HCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVARYTQDTQSITNQLAEASEHQAQEIAGASTAMNEMAQSIDQVSANASESAEVAQRSVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IASNGAQVVNRSIEGMDTIREQIQETSKRIKRLGESSQEIGNIVSLINDIADQTNILALN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEH
AAIQASMAGEAGRGFAVVADEVQRLAERSASATKQIETLVKTIQTDTNEAVISMEQTTTE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHHHH
VVRGANLAKDAGIALDEIQKVSGDLAKLIASISDAAKLQSASASHIATTMTVVQEITSQT
HHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TTATFDTARSVSELANMAESLRESVTDFKLPD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GFKLKKKSGNRGATGKSSVNFIAKIQSKIEQFASLFGESEKSKPILYAALLCLVCALIS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LAYLFYNVPRHNQLIRSLGELRLLSQTISKQATEATESGSQEAITKLQQSKKDFDENLLE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IENIHGKSTDEYQKVEKIWGEVSKNIDLISSHQRVLNQLYDTNISISETVPEIQAEYNLM
HHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VDQMARQGLPSSQVIIAKNQVFIAERILRSINSVLSGTDGNVSTSDFSVDIETFGTYLNA
HHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCC
ELNGNAELGVDRIADPALRESLESIKSEYDKVLKSAAATVLKNGSQIVNVRQASSQIFSK
EECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SDVMLESLGHLSDVARKNWLTLFLGAVLILSLGGLIFSVFKLITLRSVMDKQRVARLQEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDRNQNAILRLLDEIADLADGDLRSYATVSEDFTGAIADSINFAIDQLRDLVSRIHETSQ
HCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVARYTQDTQSITNQLAEASEHQAQEIAGASTAMNEMAQSIDQVSANASESAEVAQRSVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IASNGAQVVNRSIEGMDTIREQIQETSKRIKRLGESSQEIGNIVSLINDIADQTNILALN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEH
AAIQASMAGEAGRGFAVVADEVQRLAERSASATKQIETLVKTIQTDTNEAVISMEQTTTE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHHHH
VVRGANLAKDAGIALDEIQKVSGDLAKLIASISDAAKLQSASASHIATTMTVVQEITSQT
HHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TTATFDTARSVSELANMAESLRESVTDFKLPD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]