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The map label for this gene is gatA [H]

Identifier: 213157802

GI number: 213157802

Start: 3383418

End: 3384896

Strand: Direct

Name: gatA [H]

Synonym: AB57_3285

Alternate gene names: 213157802

Gene position: 3383418-3384896 (Clockwise)

Preceding gene: 213157801

Following gene: 213157803

Centisome position: 83.53

GC content: 43.54

Gene sequence:

>1479_bases
ATGACTGATTTACATCGCTTATCAATTCGCGAACTTGCTGAAGGCTTAAGCCAAGCGAAGTTTTCATCTCGCGAATTGAC
TGAACATTATTTAAAACGTATTGCTAAAATTGACCCGCAAGTTAAGAGCTATGTGACGGTTACTCCAGAACAGGCACTTC
GCGAAGCTGATGCTGCCGATGCTGCATTAAAAGCTGGCAATGCAACTGCTTTAACGGGTATTCCTCTTGCCCACAAAGAC
ATCTTTTGTACCAAAGGCATTAAAACCACTGCTGGTTCAAAAATGCTCGATAACTTCATTTCACCTTACGATGCAACCGT
TGTTGAAAAAACTAAAGCAGCTGGTCTGGTTACTTTAGGTAAAGTGAACATGGATGAGTTTGCGATGGGCTCAACTTCTG
AGAGTTCATATGTCGGTGCAACGAGCAACCCTTGGGCACTTGACCATGTTCCTGGCGGTTCATCTGGTGGTTCTGCTGCG
GCTGTAGCGGCTGATTTAGCACCATTTGCTACAGGTACCGATACAGGTGGCTCAATTCGCCAACCTGCTTCTTTCTGTGG
TTTAACAGGCTTAAAACCAACTTATGGTCGTGTATCGCGTTTCGGGATCATTGCTTATGCATCATCATTAGACCAAGCAG
GCCCAATGGCACGCTCTGCTGAAGACTGTGCTTACCTAATGAATGTAATTGCAGGTCACGATGCTAAAGACTCAACTTCG
GTTAAAAAAGAACTCGATGATTACGTTGCAAACCTAAATAACACATCTGTAAAAGGCTTACGCATTGGTATTCCTAAACA
ATACTTTAACGTTGCAGGTTTAGATGCTGACGTAAAAGCACGTGTTGAAGAGTCTTTGAAAAAGCTTGAGGAAATGGGCG
CAGCGCTTGTTGAGATCGACCTCAACATGACTGAAGCATATGTACCAACGTATTACCTCATCGCGCCAGCGGAAGCATCT
TCTAACTTGTCACGTTATGACGGTGTTCGCTACGGTTACCGCTGTGAAAACCCAGCTGACTTGATGGATCTTTACAAACG
TTCGCGTTCAGAAGGCTTCGGTCCAGAAGTTCAACGCCGTATTTTAATTGGTACATACGCGCTTTCTGCGGGTTATTACG
ATGCTTACTATGTAAAAGCACAAAAAGTACGTCGTTTAATTCAACAAGACTTCTTAAAAGCTTTTGAAAATGTAGATGTA
ATTGCAGCACCTGCTGCACCAACAACTGCCTACAAAATCGGTGCATCTTTAGACCCTGTTGAAATGTACTTAGGCGATAT
CTATACCATCGCAGTGAACTTAGCGGGCTTGCCAGCGATTAATGCTCCTGTTGGTTTTGATAAAGACAACTTACCAGTAG
GTTTACAGTTAATCGGTAATTACTGGTCAGAATCACAATTGTTGTCGATTGTTCACCAATACCAACAAAACACTGACTGG
CACACCAAACGTGCGGCAATTGCTGAGGAGAATGCATAA

Upstream 100 bases:

>100_bases
CTGCAACTCAAGATGGTTTGTATCTTGTTCCTCGCGTGATTGAATAACTTTTAATCAATCAACCCATCTTCCCTATTTTG
AATGTAAAGAAATTTTACTC

Downstream 100 bases:

>100_bases
TGGCTGAAGCTCAAAAGTTGAAATTGATTGACGGTTGGGAAGTCGTTATTGGTATTGAGATTCACACTCAGCTTGCAACC
AATTCTAAAATTTTCTCAGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTDLHRLSIRELAEGLSQAKFSSRELTEHYLKRIAKIDPQVKSYVTVTPEQALREADAADAALKAGNATALTGIPLAHKD
IFCTKGIKTTAGSKMLDNFISPYDATVVEKTKAAGLVTLGKVNMDEFAMGSTSESSYVGATSNPWALDHVPGGSSGGSAA
AVAADLAPFATGTDTGGSIRQPASFCGLTGLKPTYGRVSRFGIIAYASSLDQAGPMARSAEDCAYLMNVIAGHDAKDSTS
VKKELDDYVANLNNTSVKGLRIGIPKQYFNVAGLDADVKARVEESLKKLEEMGAALVEIDLNMTEAYVPTYYLIAPAEAS
SNLSRYDGVRYGYRCENPADLMDLYKRSRSEGFGPEVQRRILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDV
IAAPAAPTTAYKIGASLDPVEMYLGDIYTIAVNLAGLPAINAPVGFDKDNLPVGLQLIGNYWSESQLLSIVHQYQQNTDW
HTKRAAIAEENA

Sequences:

>Translated_492_residues
MTDLHRLSIRELAEGLSQAKFSSRELTEHYLKRIAKIDPQVKSYVTVTPEQALREADAADAALKAGNATALTGIPLAHKD
IFCTKGIKTTAGSKMLDNFISPYDATVVEKTKAAGLVTLGKVNMDEFAMGSTSESSYVGATSNPWALDHVPGGSSGGSAA
AVAADLAPFATGTDTGGSIRQPASFCGLTGLKPTYGRVSRFGIIAYASSLDQAGPMARSAEDCAYLMNVIAGHDAKDSTS
VKKELDDYVANLNNTSVKGLRIGIPKQYFNVAGLDADVKARVEESLKKLEEMGAALVEIDLNMTEAYVPTYYLIAPAEAS
SNLSRYDGVRYGYRCENPADLMDLYKRSRSEGFGPEVQRRILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDV
IAAPAAPTTAYKIGASLDPVEMYLGDIYTIAVNLAGLPAINAPVGFDKDNLPVGLQLIGNYWSESQLLSIVHQYQQNTDW
HTKRAAIAEENA
>Mature_491_residues
TDLHRLSIRELAEGLSQAKFSSRELTEHYLKRIAKIDPQVKSYVTVTPEQALREADAADAALKAGNATALTGIPLAHKDI
FCTKGIKTTAGSKMLDNFISPYDATVVEKTKAAGLVTLGKVNMDEFAMGSTSESSYVGATSNPWALDHVPGGSSGGSAAA
VAADLAPFATGTDTGGSIRQPASFCGLTGLKPTYGRVSRFGIIAYASSLDQAGPMARSAEDCAYLMNVIAGHDAKDSTSV
KKELDDYVANLNNTSVKGLRIGIPKQYFNVAGLDADVKARVEESLKKLEEMGAALVEIDLNMTEAYVPTYYLIAPAEASS
NLSRYDGVRYGYRCENPADLMDLYKRSRSEGFGPEVQRRILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDVI
AAPAAPTTAYKIGASLDPVEMYLGDIYTIAVNLAGLPAINAPVGFDKDNLPVGLQLIGNYWSESQLLSIVHQYQQNTDWH
TKRAAIAEENA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=36.144578313253, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=27.2357723577236, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI166795287, Length=170, Percent_Identity=35.2941176470588, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=441, Percent_Identity=38.7755102040816, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17556264, Length=302, Percent_Identity=28.1456953642384, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17537465, Length=243, Percent_Identity=29.2181069958848, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=238, Percent_Identity=30.672268907563, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=305, Percent_Identity=27.8688524590164, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=327, Percent_Identity=26.9113149847095, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=327, Percent_Identity=26.9113149847095, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=38.1944444444444, Blast_Score=253, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=418, Percent_Identity=29.9043062200957, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=265, Percent_Identity=28.3018867924528, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648113, Length=480, Percent_Identity=40.625, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=26.3366336633663, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652985, Length=491, Percent_Identity=26.0692464358452, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922090, Length=491, Percent_Identity=26.0692464358452, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652981, Length=491, Percent_Identity=26.0692464358452, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652983, Length=491, Percent_Identity=26.0692464358452, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45550774, Length=470, Percent_Identity=26.5957446808511, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24648435, Length=486, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24648437, Length=486, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=25.7668711656442, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161078093, Length=495, Percent_Identity=26.4646464646465, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648441, Length=420, Percent_Identity=25.952380952381, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648439, Length=420, Percent_Identity=25.952380952381, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53026; Mature: 52895

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLHRLSIRELAEGLSQAKFSSRELTEHYLKRIAKIDPQVKSYVTVTPEQALREADAAD
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHEEEECHHHHHHHHHHHH
AALKAGNATALTGIPLAHKDIFCTKGIKTTAGSKMLDNFISPYDATVVEKTKAAGLVTLG
HHHHCCCCEEEECCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEC
KVNMDEFAMGSTSESSYVGATSNPWALDHVPGGSSGGSAAAVAADLAPFATGTDTGGSIR
CCCHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
QPASFCGLTGLKPTYGRVSRFGIIAYASSLDQAGPMARSAEDCAYLMNVIAGHDAKDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHH
VKKELDDYVANLNNTSVKGLRIGIPKQYFNVAGLDADVKARVEESLKKLEEMGAALVEID
HHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
LNMTEAYVPTYYLIAPAEASSNLSRYDGVRYGYRCENPADLMDLYKRSRSEGFGPEVQRR
CCCHHHHCCEEEEEEECCCCCCHHHHCCCCCCEECCCHHHHHHHHHHHHCCCCCHHHHHE
ILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDVIAAPAAPTTAYKIGASLDPV
EEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCHH
EMYLGDIYTIAVNLAGLPAINAPVGFDKDNLPVGLQLIGNYWSESQLLSIVHQYQQNTDW
HHHHCCHHEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCC
HTKRAAIAEENA
HHHHHHHCCCCC
>Mature Secondary Structure 
TDLHRLSIRELAEGLSQAKFSSRELTEHYLKRIAKIDPQVKSYVTVTPEQALREADAAD
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHEEEECHHHHHHHHHHHH
AALKAGNATALTGIPLAHKDIFCTKGIKTTAGSKMLDNFISPYDATVVEKTKAAGLVTLG
HHHHCCCCEEEECCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEC
KVNMDEFAMGSTSESSYVGATSNPWALDHVPGGSSGGSAAAVAADLAPFATGTDTGGSIR
CCCHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
QPASFCGLTGLKPTYGRVSRFGIIAYASSLDQAGPMARSAEDCAYLMNVIAGHDAKDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHH
VKKELDDYVANLNNTSVKGLRIGIPKQYFNVAGLDADVKARVEESLKKLEEMGAALVEID
HHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
LNMTEAYVPTYYLIAPAEASSNLSRYDGVRYGYRCENPADLMDLYKRSRSEGFGPEVQRR
CCCHHHHCCEEEEEEECCCCCCHHHHCCCCCCEECCCHHHHHHHHHHHHCCCCCHHHHHE
ILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDVIAAPAAPTTAYKIGASLDPV
EEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCHH
EMYLGDIYTIAVNLAGLPAINAPVGFDKDNLPVGLQLIGNYWSESQLLSIVHQYQQNTDW
HHHHCCHHEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCC
HTKRAAIAEENA
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA