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The map label for this gene is adeK [H]

Identifier: 213157679

GI number: 213157679

Start: 3283347

End: 3284801

Strand: Direct

Name: adeK [H]

Synonym: AB57_3158

Alternate gene names: 213157679

Gene position: 3283347-3284801 (Clockwise)

Preceding gene: 213157678

Following gene: 213157680

Centisome position: 81.06

GC content: 42.27

Gene sequence:

>1455_bases
ATGCAAAAAGTATGGTCTATTTCAGGTCGTAGCATTGCGGTATCTGCACTTGCGCTTGCTTTGGCAGCTTGTCAAAGCAT
GCGCGGCCCAGAACCAGTCGTGAAAACCGATATACCACAAAGCTATGCATATAACAGCGCTTCTGGTACGTCTATTGCTG
AACAGGGTTATAAACAGTTCTTTGCTGACCCGCGTTTGCTTGAAGTGATTGATTTGGCTCTTGCCAATAACCGTGACTTA
CGTACAGCAACGCTCAATATTGAACGTGCTCAACAGCAATATCAGATTACACAGAACAACCAGCTTCCAACAATCGGTGC
AAGTGGTAGTGCAATTCGTCAGGTTTCTCAAAGCCGTGATCCGAATAACCCCTACTCTACTTATCAAGTAGGTTTGGGTG
TAACTGCTTATGAGCTAGATTTCTGGGGTCGTGTTCGTAGCCTCAAAGATGCTGCTTTAGATAGTTATCTTGCAACACAA
AGTGCTCGTGATTCGACTCAAATCAGTCTGATTAGCCAAGTTGCTCAAGCATGGTTAAATTATTCGTTTGCAACAGCAAA
CTTAAGACTGGCAGAGCAAACGCTTAAAGCACAGTTAGATTCTTACAATCTCAACAAAAAACGTTTTGATGTAGGTATCG
ACAGTGAAGTTCCATTACGTCAAGCACAGATTTCTGTAGAAACTGCGCGTAATGATGTAGCGAACTACAAAACTCAAATT
GCTCAAGCACAAAACTTGTTGAACTTGCTTGTAGGCCAACCTGTTCCACAAAACTTGTTACCTACACAACCTGTAAAACG
CATTGCTCAACAAAATGTGTTTACTGCCGGTTTACCAAGTGACTTGTTAAATAACCGTCCAGATGTAAAAGCTGCTGAAT
ACAACTTAAGCGCTGCGGGTGCGAATATCGGTGCAGCAAAAGCACGTTTATTCCCAACCATTAGCTTAACGGGTTCGGCT
GGTTATGCATCAACTGACTTAAGTGATCTATTTAAGTCTGGTGGTTTTGTCTGGTCAGTTGGTCCAAGCTTAGATTTACC
AATCTTTGACTGGGGTACACGCCGTGCCAATGTAAAAATTTCTGAAACCGATCAGAAAATTGCATTGTCTGATTATGAAA
AATCAGTTCAGTCGGCTTTCCGTGAAGTTAATGATGCACTTGCAACTCGTGCCAATATTGGCGAGCGTTTAACCGCTCAG
CAACGTCTAGTAGAAGCAACTAACCGCAACTACACACTTTCAAATGCCCGCTTCCGTGCTGGTATTGATAGTTACTTGAC
AGTTCTTGATGCACAGCGTTCTTCATATGCGGCTGAACAAGGTTTGTTATTGCTTCAACAGGCAAACTTAAACAACCAAA
TCGAGTTATACAAAACTCTAGGTGGCGGTTTAAAAGCAAATACTTCAGATACAGTGGTACATCAACCATCTAGTGCTGAA
CTTAAAAAGCAATAA

Upstream 100 bases:

>100_bases
CGTTCTTAGGTATCTTCTTTATCCCTGTATTCTATGTGTGGATTCGTAGTATCTTTAAGTACAAACCAAAAACCATAAAC
ACTCAGGAGCATAAATCGTG

Downstream 100 bases:

>100_bases
TTTTTAAATCACTTTCTTATAAAAGCTCACTTCGGTGGGCTTTTTTATTGCAACTCATCTGAATATTTTTTATCTTGAAA
GCTTAAGAAATTTAGTTTTA

Product: multidrug efflux protein AdeK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MQKVWSISGRSIAVSALALALAACQSMRGPEPVVKTDIPQSYAYNSASGTSIAEQGYKQFFADPRLLEVIDLALANNRDL
RTATLNIERAQQQYQITQNNQLPTIGASGSAIRQVSQSRDPNNPYSTYQVGLGVTAYELDFWGRVRSLKDAALDSYLATQ
SARDSTQISLISQVAQAWLNYSFATANLRLAEQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
AQAQNLLNLLVGQPVPQNLLPTQPVKRIAQQNVFTAGLPSDLLNNRPDVKAAEYNLSAAGANIGAAKARLFPTISLTGSA
GYASTDLSDLFKSGGFVWSVGPSLDLPIFDWGTRRANVKISETDQKIALSDYEKSVQSAFREVNDALATRANIGERLTAQ
QRLVEATNRNYTLSNARFRAGIDSYLTVLDAQRSSYAAEQGLLLLQQANLNNQIELYKTLGGGLKANTSDTVVHQPSSAE
LKKQ

Sequences:

>Translated_484_residues
MQKVWSISGRSIAVSALALALAACQSMRGPEPVVKTDIPQSYAYNSASGTSIAEQGYKQFFADPRLLEVIDLALANNRDL
RTATLNIERAQQQYQITQNNQLPTIGASGSAIRQVSQSRDPNNPYSTYQVGLGVTAYELDFWGRVRSLKDAALDSYLATQ
SARDSTQISLISQVAQAWLNYSFATANLRLAEQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
AQAQNLLNLLVGQPVPQNLLPTQPVKRIAQQNVFTAGLPSDLLNNRPDVKAAEYNLSAAGANIGAAKARLFPTISLTGSA
GYASTDLSDLFKSGGFVWSVGPSLDLPIFDWGTRRANVKISETDQKIALSDYEKSVQSAFREVNDALATRANIGERLTAQ
QRLVEATNRNYTLSNARFRAGIDSYLTVLDAQRSSYAAEQGLLLLQQANLNNQIELYKTLGGGLKANTSDTVVHQPSSAE
LKKQ
>Mature_484_residues
MQKVWSISGRSIAVSALALALAACQSMRGPEPVVKTDIPQSYAYNSASGTSIAEQGYKQFFADPRLLEVIDLALANNRDL
RTATLNIERAQQQYQITQNNQLPTIGASGSAIRQVSQSRDPNNPYSTYQVGLGVTAYELDFWGRVRSLKDAALDSYLATQ
SARDSTQISLISQVAQAWLNYSFATANLRLAEQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
AQAQNLLNLLVGQPVPQNLLPTQPVKRIAQQNVFTAGLPSDLLNNRPDVKAAEYNLSAAGANIGAAKARLFPTISLTGSA
GYASTDLSDLFKSGGFVWSVGPSLDLPIFDWGTRRANVKISETDQKIALSDYEKSVQSAFREVNDALATRANIGERLTAQ
QRLVEATNRNYTLSNARFRAGIDSYLTVLDAQRSSYAAEQGLLLLQQANLNNQIELYKTLGGGLKANTSDTVVHQPSSAE
LKKQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=457, Percent_Identity=37.855579868709, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI2367348, Length=426, Percent_Identity=26.2910798122066, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52803; Mature: 52803

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVWSISGRSIAVSALALALAACQSMRGPEPVVKTDIPQSYAYNSASGTSIAEQGYKQF
CCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCCCCCCCCHHHHHHHHHH
FADPRLLEVIDLALANNRDLRTATLNIERAQQQYQITQNNQLPTIGASGSAIRQVSQSRD
HCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCC
PNNPYSTYQVGLGVTAYELDFWGRVRSLKDAALDSYLATQSARDSTQISLISQVAQAWLN
CCCCCCEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
YSFATANLRLAEQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
CCEEECHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AQAQNLLNLLVGQPVPQNLLPTQPVKRIAQQNVFTAGLPSDLLNNRPDVKAAEYNLSAAG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEECCCCHHHHCCCCCCEEEEECCHHCC
ANIGAAKARLFPTISLTGSAGYASTDLSDLFKSGGFVWSVGPSLDLPIFDWGTRRANVKI
CCCCCHHHHEEEEEEECCCCCCCCCCHHHHHHCCCEEEEECCCCCCCEECCCCCEEEEEE
SETDQKIALSDYEKSVQSAFREVNDALATRANIGERLTAQQRLVEATNRNYTLSNARFRA
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHC
GIDSYLTVLDAQRSSYAAEQGLLLLQQANLNNQIELYKTLGGGLKANTSDTVVHQPSSAE
CHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEHHCCCCCCCCCCCEEECCCCCC
LKKQ
CCCC
>Mature Secondary Structure
MQKVWSISGRSIAVSALALALAACQSMRGPEPVVKTDIPQSYAYNSASGTSIAEQGYKQF
CCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCCCCCCCCHHHHHHHHHH
FADPRLLEVIDLALANNRDLRTATLNIERAQQQYQITQNNQLPTIGASGSAIRQVSQSRD
HCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCC
PNNPYSTYQVGLGVTAYELDFWGRVRSLKDAALDSYLATQSARDSTQISLISQVAQAWLN
CCCCCCEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
YSFATANLRLAEQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
CCEEECHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AQAQNLLNLLVGQPVPQNLLPTQPVKRIAQQNVFTAGLPSDLLNNRPDVKAAEYNLSAAG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEECCCCHHHHCCCCCCEEEEECCHHCC
ANIGAAKARLFPTISLTGSAGYASTDLSDLFKSGGFVWSVGPSLDLPIFDWGTRRANVKI
CCCCCHHHHEEEEEEECCCCCCCCCCHHHHHHCCCEEEEECCCCCCCEECCCCCEEEEEE
SETDQKIALSDYEKSVQSAFREVNDALATRANIGERLTAQQRLVEATNRNYTLSNARFRA
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHC
GIDSYLTVLDAQRSSYAAEQGLLLLQQANLNNQIELYKTLGGGLKANTSDTVVHQPSSAE
CHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEHHCCCCCCCCCCCEEECCCCCC
LKKQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]