Click here to switch to the map view.

The map label for this gene is adeI [H]

Identifier: 213157677

GI number: 213157677

Start: 3278908

End: 3280158

Strand: Direct

Name: adeI [H]

Synonym: AB57_3156

Alternate gene names: 213157677

Gene position: 3278908-3280158 (Clockwise)

Preceding gene: 213157676

Following gene: 213157678

Centisome position: 80.95

GC content: 42.77

Gene sequence:

>1251_bases
ATGATGTCGGCTAAGCTTTGGGCACCAGCCCTTACTGCTTGCGCATTAGCAACAAGTATCGCGCTTGTTGGTTGTAGCAA
AGGCTCCGATGAGAAACAGCAAGCTGCTGCTGCTCAGAAAATGCCGCCTGCAGAAGTAGGTGTTATTGTTGCTCAACCAC
AAAGTGTTGAACAAAGCGTTGAGCTTTCAGGCCGTACTTCAGCATATCAAATTTCTGAAGTTCGTCCTCAAACAAGTGGC
GTGATTTTAAAACGTTTATTTGCTGAAGGAAGCTATGTTCGTGAAGGTCAGGCGCTTTATGAGCTCGACTCTAGAACGAA
CCGTGCAACGTTAGAAAATGCAAAAGCATCACTCCTACAACAACAGGCAAATCTAGCTTCACTACGTACCAAGTTAAATC
GTTATAAACAACTTGTTTCTAGTAATGCTGTGTCTAAACAGGAATATGATGACTTACTTGGTCAAGTCAATGTTGCAGAA
GCACAAGTTGCAGCAGCTAAGGCTCAAGTAACAAATGCAAATGTAGATCTTGGTTATTCTACAATTCGCTCTCCTATTTC
TGGCCAATCTGGTCGTTCTTCAGTAACGGCTGGTGCTTTGGTTACTGCAAACCAGACTGACCCGTTGGTAACGATTCAAC
AATTAGATCCTATCTATGTTGATATTAATCAGTCTAGTGCTGAGTTATTGCGTTTACGTCAACAACTAAGCAAAGGCAGT
TTAAATAACAGTAACAACACGAAAGTAAAATTAAAGCTTGAAGATGGTTCTACCTATCCAATCGAAGGGCAACTTGCTTT
CTCTGACGCTTCTGTAAACCAAGATACAGGAACAATTACATTACGTGCCGTATTCTCTAACCCGAATCATTTATTGCTTC
CGGGTATGTATACCACTGCGCAAATTGTTCAGGGCGTTGTTCCAAATGCTTACCTGATTCCTCAAGCTGCCATTACTCGT
TTACCTACAGGACAAGCTGTAGCGATGCTTGTTAATGCTAAAGGGGTTGTTGAGAGCCGTCCTGTTGAAACCTCTGGTGT
TCAAGGACAAAACTGGATTGTGACTAACGGCTTAAAAGCCGGCGATAAAGTCATTGTTGATGGTGTTGCCAAAGTTAAAG
AAGGGCAAGAAGTATCAGCAAAACCTTATCAAGCTCAACCAGCAAACTCTCAAGGTGCAGCACCAAATGCTGCGAAACCG
GCTCAATCAGGTAAACCTCAAGCAGAACAGAAAGCAGCTTCAAATGCATAA

Upstream 100 bases:

>100_bases
TATTTGGATTGCACTGCTTTGGCTCTGGTTATTACAAACACAAAGTAGGTTAAGTAGAAAACAAATATATTTTTAGATTT
TATCTAAACGAGGTGGAACA

Downstream 100 bases:

>100_bases
GGGGTAGATTGAATGGCACAATTTTTTATTCATCGCCCCATATTTGCGTGGGTGATTGCATTAGTCATTATGTTGGCGGG
TATTCTTACGCTAACAAAAA

Product: multidrug efflux protein AdeI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MMSAKLWAPALTACALATSIALVGCSKGSDEKQQAAAAQKMPPAEVGVIVAQPQSVEQSVELSGRTSAYQISEVRPQTSG
VILKRLFAEGSYVREGQALYELDSRTNRATLENAKASLLQQQANLASLRTKLNRYKQLVSSNAVSKQEYDDLLGQVNVAE
AQVAAAKAQVTNANVDLGYSTIRSPISGQSGRSSVTAGALVTANQTDPLVTIQQLDPIYVDINQSSAELLRLRQQLSKGS
LNNSNNTKVKLKLEDGSTYPIEGQLAFSDASVNQDTGTITLRAVFSNPNHLLLPGMYTTAQIVQGVVPNAYLIPQAAITR
LPTGQAVAMLVNAKGVVESRPVETSGVQGQNWIVTNGLKAGDKVIVDGVAKVKEGQEVSAKPYQAQPANSQGAAPNAAKP
AQSGKPQAEQKAASNA

Sequences:

>Translated_416_residues
MMSAKLWAPALTACALATSIALVGCSKGSDEKQQAAAAQKMPPAEVGVIVAQPQSVEQSVELSGRTSAYQISEVRPQTSG
VILKRLFAEGSYVREGQALYELDSRTNRATLENAKASLLQQQANLASLRTKLNRYKQLVSSNAVSKQEYDDLLGQVNVAE
AQVAAAKAQVTNANVDLGYSTIRSPISGQSGRSSVTAGALVTANQTDPLVTIQQLDPIYVDINQSSAELLRLRQQLSKGS
LNNSNNTKVKLKLEDGSTYPIEGQLAFSDASVNQDTGTITLRAVFSNPNHLLLPGMYTTAQIVQGVVPNAYLIPQAAITR
LPTGQAVAMLVNAKGVVESRPVETSGVQGQNWIVTNGLKAGDKVIVDGVAKVKEGQEVSAKPYQAQPANSQGAAPNAAKP
AQSGKPQAEQKAASNA
>Mature_416_residues
MMSAKLWAPALTACALATSIALVGCSKGSDEKQQAAAAQKMPPAEVGVIVAQPQSVEQSVELSGRTSAYQISEVRPQTSG
VILKRLFAEGSYVREGQALYELDSRTNRATLENAKASLLQQQANLASLRTKLNRYKQLVSSNAVSKQEYDDLLGQVNVAE
AQVAAAKAQVTNANVDLGYSTIRSPISGQSGRSSVTAGALVTANQTDPLVTIQQLDPIYVDINQSSAELLRLRQQLSKGS
LNNSNNTKVKLKLEDGSTYPIEGQLAFSDASVNQDTGTITLRAVFSNPNHLLLPGMYTTAQIVQGVVPNAYLIPQAAITR
LPTGQAVAMLVNAKGVVESRPVETSGVQGQNWIVTNGLKAGDKVIVDGVAKVKEGQEVSAKPYQAQPANSQGAAPNAAKP
AQSGKPQAEQKAASNA

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=404, Percent_Identity=46.039603960396, Blast_Score=331, Evalue=7e-92,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=45.2830188679245, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1789929, Length=372, Percent_Identity=44.0860215053763, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87082036, Length=344, Percent_Identity=29.3604651162791, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87081796, Length=337, Percent_Identity=25.5192878338279, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43807; Mature: 43807

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAKLWAPALTACALATSIALVGCSKGSDEKQQAAAAQKMPPAEVGVIVAQPQSVEQSV
CCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHEEEEEECCCCHHHHH
ELSGRTSAYQISEVRPQTSGVILKRLFAEGSYVREGQALYELDSRTNRATLENAKASLLQ
HCCCCCCEEEEHHCCCCHHHHHHHHHHHCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHH
QQANLASLRTKLNRYKQLVSSNAVSKQEYDDLLGQVNVAEAQVAAAKAQVTNANVDLGYS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCCCHH
TIRSPISGQSGRSSVTAGALVTANQTDPLVTIQQLDPIYVDINQSSAELLRLRQQLSKGS
HHCCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHCCC
LNNSNNTKVKLKLEDGSTYPIEGQLAFSDASVNQDTGTITLRAVFSNPNHLLLPGMYTTA
CCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCEEEECCCHHHH
QIVQGVVPNAYLIPQAAITRLPTGQAVAMLVNAKGVVESRPVETSGVQGQNWIVTNGLKA
HHHHHCCCCCEECCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCC
GDKVIVDGVAKVKEGQEVSAKPYQAQPANSQGAAPNAAKPAQSGKPQAEQKAASNA
CCEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MMSAKLWAPALTACALATSIALVGCSKGSDEKQQAAAAQKMPPAEVGVIVAQPQSVEQSV
CCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHEEEEEECCCCHHHHH
ELSGRTSAYQISEVRPQTSGVILKRLFAEGSYVREGQALYELDSRTNRATLENAKASLLQ
HCCCCCCEEEEHHCCCCHHHHHHHHHHHCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHH
QQANLASLRTKLNRYKQLVSSNAVSKQEYDDLLGQVNVAEAQVAAAKAQVTNANVDLGYS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCCCHH
TIRSPISGQSGRSSVTAGALVTANQTDPLVTIQQLDPIYVDINQSSAELLRLRQQLSKGS
HHCCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHCCC
LNNSNNTKVKLKLEDGSTYPIEGQLAFSDASVNQDTGTITLRAVFSNPNHLLLPGMYTTA
CCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCEEEECCCHHHH
QIVQGVVPNAYLIPQAAITRLPTGQAVAMLVNAKGVVESRPVETSGVQGQNWIVTNGLKA
HHHHHCCCCCEECCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCC
GDKVIVDGVAKVKEGQEVSAKPYQAQPANSQGAAPNAAKPAQSGKPQAEQKAASNA
CCEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]