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The map label for this gene is ispB [H]

Identifier: 213157674

GI number: 213157674

Start: 3275020

End: 3275997

Strand: Direct

Name: ispB [H]

Synonym: AB57_3153

Alternate gene names: 213157674

Gene position: 3275020-3275997 (Clockwise)

Preceding gene: 213157666

Following gene: 213157675

Centisome position: 80.85

GC content: 41.92

Gene sequence:

>978_bases
ATGGTCATCGATTTTAAGCAAGACATTCTCGCTCCTGTTGCTACAGACTTTGCTGCGATGGATCACTTGATTAATGAAGG
AATTAGCTCAAAAGTCGGCTTGGTCATGTCAGTGAGTAAACATGTAGTTGAAGCTGGCGGCAAACGTATGCGCCCAATCA
TGTGTTTACTTGCAGCACGCGCTTGCGGCCTCGACAATATGCAAAATGCACAGCGTCTGGCAGCTATTATTGAAATGCTT
CATACCGCAACTTTAGTGCATGATGATGTAGTTGATGAATCTGGACTTCGCCGTGGTCGTCCTACTGCAAATGCAACATG
GAACAATCAAACGGCCGTATTGGTTGGTGACTTCCTTATTGCCCGCGCTTTTGACCTTTTGGTTGATTTAAACAACATGA
CCTTATTAAAAGACTTCTCAACAGGTACATGTGAAATTGCTGAAGGTGAGGTATTACAACTCCAATCTCAGCATCAACCG
GATACAACCGAAGAAACATATTTAAAAATTATTCACGGGAAAACTTCTCGTTTATTTGAACTTGCAACCGAAGGTGCGGC
TATTTTGGCTGGGCAAGAAGCTTACCGCGAACCTTTAAGATTATTTGCAGGTCATTTCGGAAATGCATTCCAGATTATTG
ACGATATTTTAGACTACACTTCAGATGCTGAAACATTAGGTAAAAATATTGGTGATGATTTAATGGAAGGTAAACCGACT
TTACCGTTAATTTCAGCATTAGCACATTCAACTGGTGAAGAGCATGCCATTATCAGACGCAGTATTGCGACTGGTGGTGT
TGACCAATTACCTAAAGTGATTGAAATTGTGCAAAAGTCTGGTGCTTTAGACTATTGCCAGCGCCGTGCCCAAGAAGAAA
CAGAAGCAGCACTGCAAGCCTTATCAATCTTGCCTGATACACCTTATCGTCAAGCGTTGATTAATTTAACTCGGCTTGCA
TTACATCGAATACAGTAG

Upstream 100 bases:

>100_bases
ACATACAAATGATTAATATCAATTGCTTGTTTCCCTGTTTAATGCACTTTTAATTGATACACTAACGCCACTTATCTACC
AATACGGGGTTTTTCTTCAT

Downstream 100 bases:

>100_bases
ATATTATTTTGCTTATGCATATAAATTTTTCAGAACATTTTTTTAAAATCCAGAAACAGCTTGAAAACCCTCAAGCTGTT
ATTGACGAAAATATTCTCAT

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MVIDFKQDILAPVATDFAAMDHLINEGISSKVGLVMSVSKHVVEAGGKRMRPIMCLLAARACGLDNMQNAQRLAAIIEML
HTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLIARAFDLLVDLNNMTLLKDFSTGTCEIAEGEVLQLQSQHQP
DTTEETYLKIIHGKTSRLFELATEGAAILAGQEAYREPLRLFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
LPLISALAHSTGEEHAIIRRSIATGGVDQLPKVIEIVQKSGALDYCQRRAQEETEAALQALSILPDTPYRQALINLTRLA
LHRIQ

Sequences:

>Translated_325_residues
MVIDFKQDILAPVATDFAAMDHLINEGISSKVGLVMSVSKHVVEAGGKRMRPIMCLLAARACGLDNMQNAQRLAAIIEML
HTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLIARAFDLLVDLNNMTLLKDFSTGTCEIAEGEVLQLQSQHQP
DTTEETYLKIIHGKTSRLFELATEGAAILAGQEAYREPLRLFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
LPLISALAHSTGEEHAIIRRSIATGGVDQLPKVIEIVQKSGALDYCQRRAQEETEAALQALSILPDTPYRQALINLTRLA
LHRIQ
>Mature_325_residues
MVIDFKQDILAPVATDFAAMDHLINEGISSKVGLVMSVSKHVVEAGGKRMRPIMCLLAARACGLDNMQNAQRLAAIIEML
HTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLIARAFDLLVDLNNMTLLKDFSTGTCEIAEGEVLQLQSQHQP
DTTEETYLKIIHGKTSRLFELATEGAAILAGQEAYREPLRLFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
LPLISALAHSTGEEHAIIRRSIATGGVDQLPKVIEIVQKSGALDYCQRRAQEETEAALQALSILPDTPYRQALINLTRLA
LHRIQ

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=293, Percent_Identity=32.7645051194539, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4758430, Length=267, Percent_Identity=25.0936329588015, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI83700220, Length=267, Percent_Identity=25.0936329588015, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789578, Length=313, Percent_Identity=47.9233226837061, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1786623, Length=231, Percent_Identity=31.6017316017316, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=318, Percent_Identity=29.2452830188679, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319475, Length=303, Percent_Identity=31.6831683168317, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=288, Percent_Identity=25.6944444444444, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24651612, Length=297, Percent_Identity=28.2828282828283, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281365769, Length=276, Percent_Identity=22.463768115942, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24660002, Length=276, Percent_Identity=22.463768115942, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35548; Mature: 35548

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIDFKQDILAPVATDFAAMDHLINEGISSKVGLVMSVSKHVVEAGGKRMRPIMCLLAAR
CEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ACGLDNMQNAQRLAAIIEMLHTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHH
ARAFDLLVDLNNMTLLKDFSTGTCEIAEGEVLQLQSQHQPDTTEETYLKIIHGKTSRLFE
HHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHH
LATEGAAILAGQEAYREPLRLFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
HHHCCCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCC
LPLISALAHSTGEEHAIIRRSIATGGVDQLPKVIEIVQKSGALDYCQRRAQEETEAALQA
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LSILPDTPYRQALINLTRLALHRIQ
HHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVIDFKQDILAPVATDFAAMDHLINEGISSKVGLVMSVSKHVVEAGGKRMRPIMCLLAAR
CEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ACGLDNMQNAQRLAAIIEMLHTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHH
ARAFDLLVDLNNMTLLKDFSTGTCEIAEGEVLQLQSQHQPDTTEETYLKIIHGKTSRLFE
HHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHH
LATEGAAILAGQEAYREPLRLFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
HHHCCCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCC
LPLISALAHSTGEEHAIIRRSIATGGVDQLPKVIEIVQKSGALDYCQRRAQEETEAALQA
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LSILPDTPYRQALINLTRLALHRIQ
HHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]