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The map label for this gene is cysG [H]

Identifier: 213157668

GI number: 213157668

Start: 3269306

End: 3270679

Strand: Reverse

Name: cysG [H]

Synonym: AB57_3146

Alternate gene names: 213157668

Gene position: 3270679-3269306 (Counterclockwise)

Preceding gene: 213157669

Following gene: 213157667

Centisome position: 80.75

GC content: 42.65

Gene sequence:

>1374_bases
GTGGATATTTTTCCAATCTCTTTGAAGTTGCAGCAGCAACGTTGTCTGATTGTGGGCGGAGGGCATATTGCTTTACGCAA
GGCAACCTTATTAGCTAAGGCAGGAGCAATAATTGATGTTGTTGCTCCTGCAATTGAAGATCAGCTTCTGCAATTAATTA
AAACAACAGGCGGCGAGCCGTTTATTGAAGCTTTTGCTGAAAAATTTTTAAGTACACCCTATCGTTTAGTTATTGCAGCG
ACCAATGATGCAGAAGTAAATAAAACAGTTTTTGAGCAGTGTGAAGCGCGCAATTTACTTGTAAATAGCGTCGATGATAT
TCCACATTGCCGTTTTATGGTGCCTGCGATTATTGATCGATCTCCATTAATTGTTTCTGTGGCTTCAAATGGAACATCTC
CTGTTCTATCTCGTCAAATCCGTACGCAGTTAGAAACCTCTATTCCGCACGGCATGGGCAAGTTAGCTGAGTTTTCAGGA
AAATGGCGCAATCAGGTTAAAGAAAAAATTTCTAATCCAGATGAGCGCCGTATTTTTTGGGAAAACTTGTATGCAAGCCC
CTTAAAGGAACAAGTTTTTAATGACAATCTAGATGTTGCCGATAGCATGCTTGAACAAGCACTGCAGGAGTGGAAAGCAC
CTAAAGGCGAGGTTTATTTGGTGGGTGCAGGTCCAGGTGATCCTGAACTCATTACTTTAAAAGCTTTACGTTTAATGCAG
CAAGCTGATGTGGTCATTTATGACCGTCTTGTTTCTGCACCTATTTTAGAATTGTGCCGTCGAGACGCAACAAAAATATA
TGTTGGTAAAGCGCGTTCTAACCATAGTGTTCCTCAAGAAGGTATTAATGCATTATTGGTTGACTACGCGAAAAAAGGTA
AACGTGTTTGTCGTTTAAAAGGCCGAGACCCTTTTATTTTTGGCCGTGGTGGCGAAGAGATCCAAGAGTTATTCCAAGCT
GGCGTCCCATTTCAAGTTGTTCCCGGTATTACTGCTGCGTCAGGTTGTTCAGCTTATGCAGGTATTCCGCTTACCCATCG
TGATTATGCCCAGAGTGTCCGCTTTCTTACAGGCCATCTAAAAGAAGGTTCTCCTGAGTTACCGTGGAACGAACTGGTAT
ACGAAAACCAGACATTAGTACTGTATATGGGGTTGGTGGGATTAGAGCGTATTTGTGAGCAGCTGATTGCGCATGGACAA
CGTCCAGATATGCCAGTTGCATTAATTTCAAAGGGTACGACACCTGAACAGAAGGTTGTGGTGGGGTCACTTGCTGATAT
TGCATCTAAAGTGACAGAACATCAAATTCATGCGCCGACACTCACAATTATTGGTGAGGTTGTACGTTTGCGTGAACAGT
TACAGTGGAATTAG

Upstream 100 bases:

>100_bases
CCATATATGGGTGGTGCAACGTTTATTGATTAAGCTTTGCATCTAAATTGTGCACGGTTTTTGCTTAAACATAGTCAGAT
GATTGATGTAGAGGTTTAAG

Downstream 100 bases:

>100_bases
TAGGCTTATTGATATCTGCAAAAGAGATGTAATTATTGCCTGAGCTAACCAATAAGTAATTTAAGTGAGTAATTAAGTGA
TTCATGTTGTTCTATACGAG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDIFPISLKLQQQRCLIVGGGHIALRKATLLAKAGAIIDVVAPAIEDQLLQLIKTTGGEPFIEAFAEKFLSTPYRLVIAA
TNDAEVNKTVFEQCEARNLLVNSVDDIPHCRFMVPAIIDRSPLIVSVASNGTSPVLSRQIRTQLETSIPHGMGKLAEFSG
KWRNQVKEKISNPDERRIFWENLYASPLKEQVFNDNLDVADSMLEQALQEWKAPKGEVYLVGAGPGDPELITLKALRLMQ
QADVVIYDRLVSAPILELCRRDATKIYVGKARSNHSVPQEGINALLVDYAKKGKRVCRLKGRDPFIFGRGGEEIQELFQA
GVPFQVVPGITAASGCSAYAGIPLTHRDYAQSVRFLTGHLKEGSPELPWNELVYENQTLVLYMGLVGLERICEQLIAHGQ
RPDMPVALISKGTTPEQKVVVGSLADIASKVTEHQIHAPTLTIIGEVVRLREQLQWN

Sequences:

>Translated_457_residues
MDIFPISLKLQQQRCLIVGGGHIALRKATLLAKAGAIIDVVAPAIEDQLLQLIKTTGGEPFIEAFAEKFLSTPYRLVIAA
TNDAEVNKTVFEQCEARNLLVNSVDDIPHCRFMVPAIIDRSPLIVSVASNGTSPVLSRQIRTQLETSIPHGMGKLAEFSG
KWRNQVKEKISNPDERRIFWENLYASPLKEQVFNDNLDVADSMLEQALQEWKAPKGEVYLVGAGPGDPELITLKALRLMQ
QADVVIYDRLVSAPILELCRRDATKIYVGKARSNHSVPQEGINALLVDYAKKGKRVCRLKGRDPFIFGRGGEEIQELFQA
GVPFQVVPGITAASGCSAYAGIPLTHRDYAQSVRFLTGHLKEGSPELPWNELVYENQTLVLYMGLVGLERICEQLIAHGQ
RPDMPVALISKGTTPEQKVVVGSLADIASKVTEHQIHAPTLTIIGEVVRLREQLQWN
>Mature_457_residues
MDIFPISLKLQQQRCLIVGGGHIALRKATLLAKAGAIIDVVAPAIEDQLLQLIKTTGGEPFIEAFAEKFLSTPYRLVIAA
TNDAEVNKTVFEQCEARNLLVNSVDDIPHCRFMVPAIIDRSPLIVSVASNGTSPVLSRQIRTQLETSIPHGMGKLAEFSG
KWRNQVKEKISNPDERRIFWENLYASPLKEQVFNDNLDVADSMLEQALQEWKAPKGEVYLVGAGPGDPELITLKALRLMQ
QADVVIYDRLVSAPILELCRRDATKIYVGKARSNHSVPQEGINALLVDYAKKGKRVCRLKGRDPFIFGRGGEEIQELFQA
GVPFQVVPGITAASGCSAYAGIPLTHRDYAQSVRFLTGHLKEGSPELPWNELVYENQTLVLYMGLVGLERICEQLIAHGQ
RPDMPVALISKGTTPEQKVVVGSLADIASKVTEHQIHAPTLTIIGEVVRLREQLQWN

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=458, Percent_Identity=50.6550218340611, Blast_Score=427, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6322922, Length=258, Percent_Identity=31.3953488372093, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 50494; Mature: 50494

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFPISLKLQQQRCLIVGGGHIALRKATLLAKAGAIIDVVAPAIEDQLLQLIKTTGGEP
CCEEEEEEEECCCCEEEEECCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCH
FIEAFAEKFLSTPYRLVIAATNDAEVNKTVFEQCEARNLLVNSVDDIPHCRFMVPAIIDR
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
SPLIVSVASNGTSPVLSRQIRTQLETSIPHGMGKLAEFSGKWRNQVKEKISNPDERRIFW
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
ENLYASPLKEQVFNDNLDVADSMLEQALQEWKAPKGEVYLVGAGPGDPELITLKALRLMQ
HHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEHHHHHHHHH
QADVVIYDRLVSAPILELCRRDATKIYVGKARSNHSVPQEGINALLVDYAKKGKRVCRLK
HCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GRDPFIFGRGGEEIQELFQAGVPFQVVPGITAASGCSAYAGIPLTHRDYAQSVRFLTGHL
CCCCEEECCCCHHHHHHHHCCCCEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHC
KEGSPELPWNELVYENQTLVLYMGLVGLERICEQLIAHGQRPDMPVALISKGTTPEQKVV
CCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHH
VGSLADIASKVTEHQIHAPTLTIIGEVVRLREQLQWN
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIFPISLKLQQQRCLIVGGGHIALRKATLLAKAGAIIDVVAPAIEDQLLQLIKTTGGEP
CCEEEEEEEECCCCEEEEECCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCH
FIEAFAEKFLSTPYRLVIAATNDAEVNKTVFEQCEARNLLVNSVDDIPHCRFMVPAIIDR
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
SPLIVSVASNGTSPVLSRQIRTQLETSIPHGMGKLAEFSGKWRNQVKEKISNPDERRIFW
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
ENLYASPLKEQVFNDNLDVADSMLEQALQEWKAPKGEVYLVGAGPGDPELITLKALRLMQ
HHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEHHHHHHHHH
QADVVIYDRLVSAPILELCRRDATKIYVGKARSNHSVPQEGINALLVDYAKKGKRVCRLK
HCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GRDPFIFGRGGEEIQELFQAGVPFQVVPGITAASGCSAYAGIPLTHRDYAQSVRFLTGHL
CCCCEEECCCCHHHHHHHHCCCCEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHC
KEGSPELPWNELVYENQTLVLYMGLVGLERICEQLIAHGQRPDMPVALISKGTTPEQKVV
CCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHH
VGSLADIASKVTEHQIHAPTLTIIGEVVRLREQLQWN
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA