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The map label for this gene is rrmJ [H]

Identifier: 213157620

GI number: 213157620

Start: 3205880

End: 3206530

Strand: Reverse

Name: rrmJ [H]

Synonym: AB57_3097

Alternate gene names: 213157620

Gene position: 3206530-3205880 (Counterclockwise)

Preceding gene: 213157623

Following gene: 213157619

Centisome position: 79.16

GC content: 40.4

Gene sequence:

>651_bases
ATGGCAACACGCATCACCAACCAAAAATTGTCTAAAAGTAGTCGTGCGTGGATGAGGGAACATCTAGACGATCCTTTTGT
AAAAAAAGCACAAAAGGAAGGATATCGTGCACGAGCAGCGTATAAACTTCTTGAAATTCAAGAAAAATATAAGTTGATCA
AGCCAGGCATGACAGTGGTGGACTTGGGAGCAGCTCCTGGGAGTTGGTCACAAATCGCTGGGAAACTCGTTGGTAGTAAA
GGATTGGTTATTGCTTCCGATATTTTACCTATGGATGCTTTGCCAGATGTCACTTTCTTACAAGGCGACTTCAGAGAAGA
AGCTGTATTTGAAAAATTGTTAAATATTTTAAACGGAAGACAAGTAGACATTGTAATTTCTGATATGGCCCCCAATACAT
CAGGTAATAGGGCTGTTGATCAACCTCGACAGATTTACTTGTGTGAACTTGCTTTAGACTTTGCTCAGAAAGTCTTGGGT
CCAAATGGACAGTTTGTTGTTAAAGTGTTCCAAGGCGCAGGATTTGATGAGTTTCGTAAACAAGTCGTTGATAGTTTTGA
TGTGTTAAAAACAGCAAAACCAGCAGCTTCTCGGGCACGATCTAAAGAAGTTTTTTTGGTTGGACAAGGGCGTAAGAAAG
CATTGCAATAA

Upstream 100 bases:

>100_bases
AACTGTAAAAGTTATTGAGATTACGTTACATCCGTACTGTAATGAGAGTAATTTTCACGTACAATGAGCAATCAGAAGTT
TTTTTGATATTTAGTGAGTT

Downstream 100 bases:

>100_bases
AGTTTACTTGCCAAGCTCCTAAAAATTGTGCATTTTGTATCTTTTAGGTTATCCGCAAGCAAGACAATTGCTGTTTTTAA
GACGATCGGCCAAGTTTGGG

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MATRITNQKLSKSSRAWMREHLDDPFVKKAQKEGYRARAAYKLLEIQEKYKLIKPGMTVVDLGAAPGSWSQIAGKLVGSK
GLVIASDILPMDALPDVTFLQGDFREEAVFEKLLNILNGRQVDIVISDMAPNTSGNRAVDQPRQIYLCELALDFAQKVLG
PNGQFVVKVFQGAGFDEFRKQVVDSFDVLKTAKPAASRARSKEVFLVGQGRKKALQ

Sequences:

>Translated_216_residues
MATRITNQKLSKSSRAWMREHLDDPFVKKAQKEGYRARAAYKLLEIQEKYKLIKPGMTVVDLGAAPGSWSQIAGKLVGSK
GLVIASDILPMDALPDVTFLQGDFREEAVFEKLLNILNGRQVDIVISDMAPNTSGNRAVDQPRQIYLCELALDFAQKVLG
PNGQFVVKVFQGAGFDEFRKQVVDSFDVLKTAKPAASRARSKEVFLVGQGRKKALQ
>Mature_215_residues
ATRITNQKLSKSSRAWMREHLDDPFVKKAQKEGYRARAAYKLLEIQEKYKLIKPGMTVVDLGAAPGSWSQIAGKLVGSKG
LVIASDILPMDALPDVTFLQGDFREEAVFEKLLNILNGRQVDIVISDMAPNTSGNRAVDQPRQIYLCELALDFAQKVLGP
NGQFVVKVFQGAGFDEFRKQVVDSFDVLKTAKPAASRARSKEVFLVGQGRKKALQ

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=228, Percent_Identity=35.5263157894737, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI29029591, Length=189, Percent_Identity=37.037037037037, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI29029589, Length=189, Percent_Identity=37.037037037037, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI7110661, Length=189, Percent_Identity=37.037037037037, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI194097365, Length=187, Percent_Identity=33.6898395721925, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=55.2884615384615, Blast_Score=234, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17553474, Length=203, Percent_Identity=37.9310344827586, Blast_Score=133, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17554650, Length=187, Percent_Identity=32.620320855615, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=186, Percent_Identity=32.258064516129, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=186, Percent_Identity=32.258064516129, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=186, Percent_Identity=32.258064516129, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319535, Length=197, Percent_Identity=34.5177664974619, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319796, Length=188, Percent_Identity=36.1702127659575, Blast_Score=118, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321302, Length=125, Percent_Identity=33.6, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356387, Length=213, Percent_Identity=38.9671361502347, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI18859957, Length=187, Percent_Identity=32.620320855615, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24647580, Length=194, Percent_Identity=32.9896907216495, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648639, Length=194, Percent_Identity=32.9896907216495, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23927; Mature: 23796

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRITNQKLSKSSRAWMREHLDDPFVKKAQKEGYRARAAYKLLEIQEKYKLIKPGMTVV
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEE
DLGAAPGSWSQIAGKLVGSKGLVIASDILPMDALPDVTFLQGDFREEAVFEKLLNILNGR
ECCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCC
QVDIVISDMAPNTSGNRAVDQPRQIYLCELALDFAQKVLGPNGQFVVKVFQGAGFDEFRK
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
QVVDSFDVLKTAKPAASRARSKEVFLVGQGRKKALQ
HHHHHHHHHHHCCHHHHHCCCCEEEEEECCHHHCCC
>Mature Secondary Structure 
ATRITNQKLSKSSRAWMREHLDDPFVKKAQKEGYRARAAYKLLEIQEKYKLIKPGMTVV
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEE
DLGAAPGSWSQIAGKLVGSKGLVIASDILPMDALPDVTFLQGDFREEAVFEKLLNILNGR
ECCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCC
QVDIVISDMAPNTSGNRAVDQPRQIYLCELALDFAQKVLGPNGQFVVKVFQGAGFDEFRK
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
QVVDSFDVLKTAKPAASRARSKEVFLVGQGRKKALQ
HHHHHHHHHHHCCHHHHHCCCCEEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA