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The map label for this gene is kgtP [H]

Identifier: 213157611

GI number: 213157611

Start: 3195893

End: 3197212

Strand: Direct

Name: kgtP [H]

Synonym: AB57_3088

Alternate gene names: 213157611

Gene position: 3195893-3197212 (Clockwise)

Preceding gene: 213158355

Following gene: 213157612

Centisome position: 78.9

GC content: 40.23

Gene sequence:

>1320_bases
ATGGTTGATCAACCTTCTACCGCAACAACTCCACATTCTAATTTAGATACAAAAACCCGTCTAAAATCAATTTTGGGCGG
TTCTGCCGGTAACCTTGTCGAATGGTACGACTGGTATGTATATGCCGCATTTACGCTCTATTTTGCTCATGCATTTTTCC
CAAAAGGAAGTCAAACCGCCCAACTTCTTCAAGCTGCCGCTATTTTTGCAGTGGGTTTCCTTATGCGTCCCATTGGCGCA
TGGATCATGGGGATCTATTCTGACCGTAAAGGACGTAAAGCAGGCCTTACGCTTTCCGTTACTTTAATGTGTATCGGTTC
GCTAATGATTGCTGTTACACCTTCCTACGAAAGTATTGGCGTATTTGCTCCATTGCTTTTGGTTATTGCACGTTTAATTC
AAGGCTTAAGTGTAGGCGGTGAATATGGCGCAAGTGCAACGTATTTAAGTGAAATGGCAGAAAAAGATCGACGTGGTTTT
TTCTCAAGCTTTCAATATGTCACTTTAATTGCAGGACAGCTTACAGCTTTATGCGTACTACTCATCTTGCAAATGATACT
TACCGAAGAACAACTGCATGACTGGGGATGGCGTGTTCCGTTTTTCATCGGAGCCCTTTTAGCTATTGTAGTGTTCCGTA
TTCGTCGTGGCTTATTGGAAACTCAATCCTTTAAAAACGCTCAAGCAGAAACAGATCAGCCGAAATCAGGAATGTTTGCT
TTATTTAAACATTATCCTAAAGAGGCCTTCACTGTATTATTCCTAACTGCTGGTGGTACTTTAGCTTTTTATACTTACAC
CACCTATTTACAGAAATATTTAGTGAATACTTCTGGTTTTACCAAGCCTGAGGCTACTCAAATTACCACTTTAGCCTTAT
TCATCTTTATGTGCTTACAGCCATTGGCAGGTGCCTTATCAGATCGAATTGGCCGTAAACCGCTTATGATCGCCTTCGGG
GTCACAGGTGTTTTATTCACTTATATTTTGTTTGATACACTTGCAAACACGCATAACTACTGGACTGCTTTCTGGCTATG
TTTAGGTGGACTGGTTATGGTAACCGGCTACACATCAATTAATGCTGTAGTGAAAGCTGAACTTTTCCCTGCACATATTC
GAGCTTTAGGTGTCGCATTACCTTATGCAATTGCGAATACTTTATTTGGTGGTACAGCCGAGTTTTTTGCATTAAGTTTT
AAAGAAGCCGGGCACGAATCTTGGTTCTTTATTTACGTCAGTATCATGATTTTCATTTCATTGCTGATTTATATCTTCAT
GAAAGACACCAAGCATCATTCAAAAATTAAAGAACATTAA

Upstream 100 bases:

>100_bases
ATAAAAAAAATAAAGTGAAATACTCCTGTAGATAATATGTAAAAACGGAATAAATCTTCTCTTTTTTAGCATTCAACCCA
AGCGAAACATGGAGCGAATC

Downstream 100 bases:

>100_bases
AATCCTTCAATTAGAGCTAATATCATAAGTGAATGTTAGCTCTAACTTAGTGAATAAGCTCCAACAACGTTTCTATTTTA
TGAGATACTTCATATAAGAA

Product: MFS permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MVDQPSTATTPHSNLDTKTRLKSILGGSAGNLVEWYDWYVYAAFTLYFAHAFFPKGSQTAQLLQAAAIFAVGFLMRPIGA
WIMGIYSDRKGRKAGLTLSVTLMCIGSLMIAVTPSYESIGVFAPLLLVIARLIQGLSVGGEYGASATYLSEMAEKDRRGF
FSSFQYVTLIAGQLTALCVLLILQMILTEEQLHDWGWRVPFFIGALLAIVVFRIRRGLLETQSFKNAQAETDQPKSGMFA
LFKHYPKEAFTVLFLTAGGTLAFYTYTTYLQKYLVNTSGFTKPEATQITTLALFIFMCLQPLAGALSDRIGRKPLMIAFG
VTGVLFTYILFDTLANTHNYWTAFWLCLGGLVMVTGYTSINAVVKAELFPAHIRALGVALPYAIANTLFGGTAEFFALSF
KEAGHESWFFIYVSIMIFISLLIYIFMKDTKHHSKIKEH

Sequences:

>Translated_439_residues
MVDQPSTATTPHSNLDTKTRLKSILGGSAGNLVEWYDWYVYAAFTLYFAHAFFPKGSQTAQLLQAAAIFAVGFLMRPIGA
WIMGIYSDRKGRKAGLTLSVTLMCIGSLMIAVTPSYESIGVFAPLLLVIARLIQGLSVGGEYGASATYLSEMAEKDRRGF
FSSFQYVTLIAGQLTALCVLLILQMILTEEQLHDWGWRVPFFIGALLAIVVFRIRRGLLETQSFKNAQAETDQPKSGMFA
LFKHYPKEAFTVLFLTAGGTLAFYTYTTYLQKYLVNTSGFTKPEATQITTLALFIFMCLQPLAGALSDRIGRKPLMIAFG
VTGVLFTYILFDTLANTHNYWTAFWLCLGGLVMVTGYTSINAVVKAELFPAHIRALGVALPYAIANTLFGGTAEFFALSF
KEAGHESWFFIYVSIMIFISLLIYIFMKDTKHHSKIKEH
>Mature_439_residues
MVDQPSTATTPHSNLDTKTRLKSILGGSAGNLVEWYDWYVYAAFTLYFAHAFFPKGSQTAQLLQAAAIFAVGFLMRPIGA
WIMGIYSDRKGRKAGLTLSVTLMCIGSLMIAVTPSYESIGVFAPLLLVIARLIQGLSVGGEYGASATYLSEMAEKDRRGF
FSSFQYVTLIAGQLTALCVLLILQMILTEEQLHDWGWRVPFFIGALLAIVVFRIRRGLLETQSFKNAQAETDQPKSGMFA
LFKHYPKEAFTVLFLTAGGTLAFYTYTTYLQKYLVNTSGFTKPEATQITTLALFIFMCLQPLAGALSDRIGRKPLMIAFG
VTGVLFTYILFDTLANTHNYWTAFWLCLGGLVMVTGYTSINAVVKAELFPAHIRALGVALPYAIANTLFGGTAEFFALSF
KEAGHESWFFIYVSIMIFISLLIYIFMKDTKHHSKIKEH

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=430, Percent_Identity=51.1627906976744, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=29.2740046838407, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788292, Length=438, Percent_Identity=30.365296803653, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1789941, Length=439, Percent_Identity=31.8906605922551, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI87082231, Length=262, Percent_Identity=26.7175572519084, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082404, Length=334, Percent_Identity=25.1497005988024, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48741; Mature: 48741

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]