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The map label for this gene is pcaF [H]

Identifier: 213157495

GI number: 213157495

Start: 2266270

End: 2267484

Strand: Direct

Name: pcaF [H]

Synonym: AB57_2184

Alternate gene names: 213157495

Gene position: 2266270-2267484 (Clockwise)

Preceding gene: 213157494

Following gene: 213157496

Centisome position: 55.95

GC content: 48.64

Gene sequence:

>1215_bases
ATGAAAAACGCTTATATCATCGATGCCATCCGTACTCCATTTGGCCGTTACGCCGGTGGCCTTGCACCTGTCCGTGCCGA
TGACCTTGGCGCTGTGCCGCTTAAAGCGCTCATGCAGCGTAACCCAAGTGTAGATTGGGAACAGGTCGATGATGTGATTT
ATGGCTGTGCCAATCAAGCCGGTGAAGATAACCGTAATGTCGGCCGTATGTCAGCACTACTTGCTGGTTTACCGTATCAG
GTGCCGGCAACCACCATTAACCGTTTATGCGGTTCATCACTCGATGCTATTGCTATTGCTGCCCGTGCCATTAAAGCCGG
CGAAGCGAACTTGGTAATTGCTGGTGGTGTGGAAAGCATGAGCCGTGCACCTTATGTGATGGGTAAGTCTGACAGTGCTT
TTGGCCGTAGCCAGAAGATTGAAGACACCACTATGGGATGGCGCTTCATTAACCCTAAACTTAAAGAATTGTATGGTGTA
GACACCATGCCCCAGACTGCCGAAAACGTGGCTGAACAGTTCAACGTGAATCGTGCAGATCAGGACCAGTTTGCCTTGGT
GAGCCAACAACGCACCGCAAGCGCGCAAGCCAAAGGCTTTTTTTCTAAAGAAATCGTGGCAGTTGAAATCCCTCAGCGTA
AGGGTGATGCTGTTGTGATTGATACCGACGAACATCCACGTGCATCGACCACCCTTGAAGCTTTAAGCAAACTTAAACCT
GTTGTAAAAGCAGAGGGCACGGTCACTGCGGGTAATGCTTCAGGCATCAACGATGGTGCAGCAGCTCTATTGATTGCTTC
TGATGATGCAGTTCAGGCCTACAATCTCAAACCACGTGCCAAGATCATTGCTTCAACAGCCGTCGGTGTAGAACCACGCA
TTATGGGCTTTGCTCCAGCACCAGCGATTAAAAAATTACTTAAACAAGCCAACCTGACTTTAGATCAGATGGATGTGATT
GAGCTCAATGAAGCCTTTGCTGCACAAGCTTTGGCAGTGACCCGTGACTTAGGCTTACCAGACAACTCTGACAAGGTAAA
TCCAAATGGTGGTGCCATTGCTTTAGGTCATCCGCTTGGTGCATCAGGTGCACGCTTAGTCACTACAGCCTTAAACCAGC
TTGAACAGACAGGTGGTCGTTATGCCTTGTGTTCAATGTGTATTGGTGTAGGCCAAGGCATTGCTTTGATTATTGAACGT
GTTGAAGCACTTTAA

Upstream 100 bases:

>100_bases
TACTCCTAATTTAGTAGTGACGATGAGCTCCAGCAAATGCTGAAAAAGAGTCTAACTATGTGGCAATGACAGCAACAGAT
TTCAAAATGGAAATGTAAGA

Downstream 100 bases:

>100_bases
GGAGAAACAAATGCCATCTTTTCAATCTGCCGATGCACAAATTAACTATCAAACTTTTGGCGAACCGTCGTCCCCTGCTC
TGGTGTTTTCCAATTCTTTG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKNAYIIDAIRTPFGRYAGGLAPVRADDLGAVPLKALMQRNPSVDWEQVDDVIYGCANQAGEDNRNVGRMSALLAGLPYQ
VPATTINRLCGSSLDAIAIAARAIKAGEANLVIAGGVESMSRAPYVMGKSDSAFGRSQKIEDTTMGWRFINPKLKELYGV
DTMPQTAENVAEQFNVNRADQDQFALVSQQRTASAQAKGFFSKEIVAVEIPQRKGDAVVIDTDEHPRASTTLEALSKLKP
VVKAEGTVTAGNASGINDGAAALLIASDDAVQAYNLKPRAKIIASTAVGVEPRIMGFAPAPAIKKLLKQANLTLDQMDVI
ELNEAFAAQALAVTRDLGLPDNSDKVNPNGGAIALGHPLGASGARLVTTALNQLEQTGGRYALCSMCIGVGQGIALIIER
VEAL

Sequences:

>Translated_404_residues
MKNAYIIDAIRTPFGRYAGGLAPVRADDLGAVPLKALMQRNPSVDWEQVDDVIYGCANQAGEDNRNVGRMSALLAGLPYQ
VPATTINRLCGSSLDAIAIAARAIKAGEANLVIAGGVESMSRAPYVMGKSDSAFGRSQKIEDTTMGWRFINPKLKELYGV
DTMPQTAENVAEQFNVNRADQDQFALVSQQRTASAQAKGFFSKEIVAVEIPQRKGDAVVIDTDEHPRASTTLEALSKLKP
VVKAEGTVTAGNASGINDGAAALLIASDDAVQAYNLKPRAKIIASTAVGVEPRIMGFAPAPAIKKLLKQANLTLDQMDVI
ELNEAFAAQALAVTRDLGLPDNSDKVNPNGGAIALGHPLGASGARLVTTALNQLEQTGGRYALCSMCIGVGQGIALIIER
VEAL
>Mature_404_residues
MKNAYIIDAIRTPFGRYAGGLAPVRADDLGAVPLKALMQRNPSVDWEQVDDVIYGCANQAGEDNRNVGRMSALLAGLPYQ
VPATTINRLCGSSLDAIAIAARAIKAGEANLVIAGGVESMSRAPYVMGKSDSAFGRSQKIEDTTMGWRFINPKLKELYGV
DTMPQTAENVAEQFNVNRADQDQFALVSQQRTASAQAKGFFSKEIVAVEIPQRKGDAVVIDTDEHPRASTTLEALSKLKP
VVKAEGTVTAGNASGINDGAAALLIASDDAVQAYNLKPRAKIIASTAVGVEPRIMGFAPAPAIKKLLKQANLTLDQMDVI
ELNEAFAAQALAVTRDLGLPDNSDKVNPNGGAIALGHPLGASGARLVTTALNQLEQTGGRYALCSMCIGVGQGIALIIER
VEAL

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=401, Percent_Identity=44.3890274314214, Blast_Score=317, Evalue=9e-87,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=43.21608040201, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI4501853, Length=407, Percent_Identity=39.3120393120393, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=36.2282878411911, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=33.3333333333333, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI194353979, Length=403, Percent_Identity=30.7692307692308, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=62.8428927680798, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=42.8217821782178, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87082165, Length=404, Percent_Identity=43.5643564356436, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1788554, Length=410, Percent_Identity=45.1219512195122, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1788683, Length=430, Percent_Identity=30, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=46.2686567164179, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=33.5820895522388, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=34.7222222222222, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17535921, Length=402, Percent_Identity=32.5870646766169, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=29.8765432098765, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325229, Length=410, Percent_Identity=37.5609756097561, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6322031, Length=410, Percent_Identity=36.8292682926829, Blast_Score=219, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=46.3840399002494, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=41.6873449131514, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24640423, Length=397, Percent_Identity=36.272040302267, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137578, Length=431, Percent_Identity=33.6426914153132, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42583; Mature: 42583

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAYIIDAIRTPFGRYAGGLAPVRADDLGAVPLKALMQRNPSVDWEQVDDVIYGCANQA
CCCCEEEHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVGRMSALLAGLPYQVPATTINRLCGSSLDAIAIAARAIKAGEANLVIAGGVESM
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPYVMGKSDSAFGRSQKIEDTTMGWRFINPKLKELYGVDTMPQTAENVAEQFNVNRAD
HCCCEEECCCCCCCCCCCCCCHHHCCEEECCCHHHHHHCCCCCCHHHHHHHHHHCCCCCC
QDQFALVSQQRTASAQAKGFFSKEIVAVEIPQRKGDAVVIDTDEHPRASTTLEALSKLKP
CHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCH
VVKAEGTVTAGNASGINDGAAALLIASDDAVQAYNLKPRAKIIASTAVGVEPRIMGFAPA
HHHCCCEEEECCCCCCCCCCEEEEEECCCCEEEECCCCHHHEEEHHHCCCCCCEEECCCC
PAIKKLLKQANLTLDQMDVIELNEAFAAQALAVTRDLGLPDNSDKVNPNGGAIALGHPLG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
ASGARLVTTALNQLEQTGGRYALCSMCIGVGQGIALIIERVEAL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKNAYIIDAIRTPFGRYAGGLAPVRADDLGAVPLKALMQRNPSVDWEQVDDVIYGCANQA
CCCCEEEHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVGRMSALLAGLPYQVPATTINRLCGSSLDAIAIAARAIKAGEANLVIAGGVESM
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPYVMGKSDSAFGRSQKIEDTTMGWRFINPKLKELYGVDTMPQTAENVAEQFNVNRAD
HCCCEEECCCCCCCCCCCCCCHHHCCEEECCCHHHHHHCCCCCCHHHHHHHHHHCCCCCC
QDQFALVSQQRTASAQAKGFFSKEIVAVEIPQRKGDAVVIDTDEHPRASTTLEALSKLKP
CHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCH
VVKAEGTVTAGNASGINDGAAALLIASDDAVQAYNLKPRAKIIASTAVGVEPRIMGFAPA
HHHCCCEEEECCCCCCCCCCEEEEEECCCCEEEECCCCHHHEEEHHHCCCCCCEEECCCC
PAIKKLLKQANLTLDQMDVIELNEAFAAQALAVTRDLGLPDNSDKVNPNGGAIALGHPLG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
ASGARLVTTALNQLEQTGGRYALCSMCIGVGQGIALIIERVEAL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125318 [H]