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The map label for this gene is cobS [H]

Identifier: 213157205

GI number: 213157205

Start: 1979328

End: 1980050

Strand: Direct

Name: cobS [H]

Synonym: AB57_1888

Alternate gene names: 213157205

Gene position: 1979328-1980050 (Clockwise)

Preceding gene: 213157204

Following gene: 213157210

Centisome position: 48.87

GC content: 37.48

Gene sequence:

>723_bases
TTGCAATTTTTAACTGTTCTGCCTATTGAGCTAAAAACAATACCAACGGCGCAACAAAATGGTCGAGCTATTCTGTTTTA
TCCTCTAGTTGGTCTGATTATTGGGGGCATACTTTTTCTTGTTACCTGTATTTTTGTTAAATTACCCGCTCTTTTACTTG
CGGCAATCGTACTCGCTTTATGGATCTGGCTGACAGGTGGATTGCATTTAGATGGGTTAGCAGATACTGCCGATGCGTGG
GTAGGCGGTTTTGGAGATAAACTACGTACTTTACAAATCATGAAGGATCCAAGTTGTGGTCCAATTGGAGTTCTCAGCTT
AGTTATCATATGTTTGCTCAAATTTACACTGATTTATGTATTGATTGAACAGCATCAATCTTTATTTTTAATATACATTC
CTATCCTTGGACGTGTGGTGCCATCTATTATGTTTTTAACTACACCATATGTCCGCGAAAAAGGCTTAGGCCGTTCTTTG
ACAGAATATTTACCCAAAACTGCTTCATGGATAATAACTGGGTTCGTTCTGCTATTACCTTTGTATTGGGGGTTGCAAGG
GCTTATTGCCATCAATAGTTTTTTAATCAGTCTTGTTTATTTAAGACATGTGTTTATAAAAAGGATTGGCGGTATTACAG
GGGATACCGTTGGTGCTGCGATTGAAATAGGAGAAACTGTACTGATGTTTACTTTTGTCGTAAGTAATTTTTATTTAGTT
TGA

Upstream 100 bases:

>100_bases
GAATAGGGATTGCTATTCCCATTTTTATTTCTTCATAATTCAATTTGTTTTTAAATATAGACCAGTTAGAGACTTGGATA
TGACACCTTTTTGGATAGCA

Downstream 100 bases:

>100_bases
TGTTGAATTCCACCTCAATAAAAATAAAAAAGCCCTCAAAAAATGAGGGCTTAATTTTCAATCTTAAAAAGATGAATTAG
CGGTTTGGTAAAACTTCTTT

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MQFLTVLPIELKTIPTAQQNGRAILFYPLVGLIIGGILFLVTCIFVKLPALLLAAIVLALWIWLTGGLHLDGLADTADAW
VGGFGDKLRTLQIMKDPSCGPIGVLSLVIICLLKFTLIYVLIEQHQSLFLIYIPILGRVVPSIMFLTTPYVREKGLGRSL
TEYLPKTASWIITGFVLLLPLYWGLQGLIAINSFLISLVYLRHVFIKRIGGITGDTVGAAIEIGETVLMFTFVVSNFYLV

Sequences:

>Translated_240_residues
MQFLTVLPIELKTIPTAQQNGRAILFYPLVGLIIGGILFLVTCIFVKLPALLLAAIVLALWIWLTGGLHLDGLADTADAW
VGGFGDKLRTLQIMKDPSCGPIGVLSLVIICLLKFTLIYVLIEQHQSLFLIYIPILGRVVPSIMFLTTPYVREKGLGRSL
TEYLPKTASWIITGFVLLLPLYWGLQGLIAINSFLISLVYLRHVFIKRIGGITGDTVGAAIEIGETVLMFTFVVSNFYLV
>Mature_240_residues
MQFLTVLPIELKTIPTAQQNGRAILFYPLVGLIIGGILFLVTCIFVKLPALLLAAIVLALWIWLTGGLHLDGLADTADAW
VGGFGDKLRTLQIMKDPSCGPIGVLSLVIICLLKFTLIYVLIEQHQSLFLIYIPILGRVVPSIMFLTTPYVREKGLGRSL
TEYLPKTASWIITGFVLLLPLYWGLQGLIAINSFLISLVYLRHVFIKRIGGITGDTVGAAIEIGETVLMFTFVVSNFYLV

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=244, Percent_Identity=31.1475409836066, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26434; Mature: 26434

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLTVLPIELKTIPTAQQNGRAILFYPLVGLIIGGILFLVTCIFVKLPALLLAAIVLAL
CCCEEEECEEHEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WIWLTGGLHLDGLADTADAWVGGFGDKLRTLQIMKDPSCGPIGVLSLVIICLLKFTLIYV
HHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LIEQHQSLFLIYIPILGRVVPSIMFLTTPYVREKGLGRSLTEYLPKTASWIITGFVLLLP
HHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHH
LYWGLQGLIAINSFLISLVYLRHVFIKRIGGITGDTVGAAIEIGETVLMFTFVVSNFYLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFLTVLPIELKTIPTAQQNGRAILFYPLVGLIIGGILFLVTCIFVKLPALLLAAIVLAL
CCCEEEECEEHEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WIWLTGGLHLDGLADTADAWVGGFGDKLRTLQIMKDPSCGPIGVLSLVIICLLKFTLIYV
HHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LIEQHQSLFLIYIPILGRVVPSIMFLTTPYVREKGLGRSLTEYLPKTASWIITGFVLLLP
HHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHH
LYWGLQGLIAINSFLISLVYLRHVFIKRIGGITGDTVGAAIEIGETVLMFTFVVSNFYLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA