Click here to switch to the map view.

The map label for this gene is pyrF

Identifier: 213157138

GI number: 213157138

Start: 1902488

End: 1903186

Strand: Direct

Name: pyrF

Synonym: AB57_1821

Alternate gene names: 213157138

Gene position: 1902488-1903186 (Clockwise)

Preceding gene: 213157136

Following gene: 213157140

Centisome position: 46.97

GC content: 37.48

Gene sequence:

>699_bases
ATGGAAGAAAGTCTCTTGAGTATCATTGTTGCGTTGGACGCTAAAAGCCAATATGACGCTTTAAAAATTGTTGAACAACT
TGATCCTACTTTATGTCGCGTAAAAGTAGGTAAAGAGCTTTTTACTCATGAAGGCCCATCTGTTGTAAAAAAACTTCAAG
AAGAAAATTTCGAAGTTTTCCTAGATTTAAAATTCCATGATATTCCAAATACGACGGCTCAAGCTGTATGTGCTGCCGCA
GACTTAGGTGTATGGATGGTTAACGTACATGCATCAGGTGGCCGTAAAATGATGGAAACCTGTGTGGAGCGTTTAAAAGC
AGGTAATTATCAAACACAATTAATTGCGGTAACAGTTTTAACTTCTATGGGGCGTGAAGATTTAAAAGATATCGGGTTAG
ATATTGAACCTGTAGAACAAGTTAAAAGATTGGCGAAACTTACTAAAGAAAGTGGTTTAGATGGTGTAGTTTGTTCTGCT
CAAGAAGCTAAAATTCTTCGTGAGTTAATCGGACAAGATTTCTCTTTAGTTACTCCGGGAATACGTCCTGAGGGTAGTAA
TGCAGATGACCAAAAACGTATTGTAACTCCGAAACAGGCAATGCTTGATGGGTCTACACATTTAGTTATTGGTCGTCCGA
TTACTAACGCAGAAAATCCAACTGAAATGTTGAAATCAATTCTTGCTTCAATTGCTTAA

Upstream 100 bases:

>100_bases
TTCTAGTTTAAAATCACTATTTCCTCATTTAAGAACTTGGTTCAATAGATTTCTTTATTACTGGCTGACTTGTCTATTAT
AATAGCGCTGATTTTATGTG

Downstream 100 bases:

>100_bases
AAGTTTAAAAACACAGTAATTAAAAGTGGAGCGAGTAGACTTAAGAGTAAGCCGCTCATCATGGCATGTGGTACATAATG
TGTGCCACATGACTGTTTTA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MEESLLSIIVALDAKSQYDALKIVEQLDPTLCRVKVGKELFTHEGPSVVKKLQEENFEVFLDLKFHDIPNTTAQAVCAAA
DLGVWMVNVHASGGRKMMETCVERLKAGNYQTQLIAVTVLTSMGREDLKDIGLDIEPVEQVKRLAKLTKESGLDGVVCSA
QEAKILRELIGQDFSLVTPGIRPEGSNADDQKRIVTPKQAMLDGSTHLVIGRPITNAENPTEMLKSILASIA

Sequences:

>Translated_232_residues
MEESLLSIIVALDAKSQYDALKIVEQLDPTLCRVKVGKELFTHEGPSVVKKLQEENFEVFLDLKFHDIPNTTAQAVCAAA
DLGVWMVNVHASGGRKMMETCVERLKAGNYQTQLIAVTVLTSMGREDLKDIGLDIEPVEQVKRLAKLTKESGLDGVVCSA
QEAKILRELIGQDFSLVTPGIRPEGSNADDQKRIVTPKQAMLDGSTHLVIGRPITNAENPTEMLKSILASIA
>Mature_232_residues
MEESLLSIIVALDAKSQYDALKIVEQLDPTLCRVKVGKELFTHEGPSVVKKLQEENFEVFLDLKFHDIPNTTAQAVCAAA
DLGVWMVNVHASGGRKMMETCVERLKAGNYQTQLIAVTVLTSMGREDLKDIGLDIEPVEQVKRLAKLTKESGLDGVVCSA
QEAKILRELIGQDFSLVTPGIRPEGSNADDQKRIVTPKQAMLDGSTHLVIGRPITNAENPTEMLKSILASIA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=225, Percent_Identity=57.3333333333333, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_ACIB5 (B7I534)

Other databases:

- EMBL:   CP001182
- RefSeq:   YP_002319182.1
- GeneID:   7044901
- GenomeReviews:   CP001182_GR
- KEGG:   abn:AB57_1821
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25353; Mature: 25353

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 64-64 BINDING 13-13 BINDING 35-35 BINDING 121-121 BINDING 182-182 BINDING 191-191 BINDING 211-211 BINDING 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEESLLSIIVALDAKSQYDALKIVEQLDPTLCRVKVGKELFTHEGPSVVKKLQEENFEVF
CCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
LDLKFHDIPNTTAQAVCAAADLGVWMVNVHASGGRKMMETCVERLKAGNYQTQLIAVTVL
EEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
TSMGREDLKDIGLDIEPVEQVKRLAKLTKESGLDGVVCSAQEAKILRELIGQDFSLVTPG
HHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCEEECCC
IRPEGSNADDQKRIVTPKQAMLDGSTHLVIGRPITNAENPTEMLKSILASIA
CCCCCCCCCCCHHCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEESLLSIIVALDAKSQYDALKIVEQLDPTLCRVKVGKELFTHEGPSVVKKLQEENFEVF
CCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
LDLKFHDIPNTTAQAVCAAADLGVWMVNVHASGGRKMMETCVERLKAGNYQTQLIAVTVL
EEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
TSMGREDLKDIGLDIEPVEQVKRLAKLTKESGLDGVVCSAQEAKILRELIGQDFSLVTPG
HHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCEEECCC
IRPEGSNADDQKRIVTPKQAMLDGSTHLVIGRPITNAENPTEMLKSILASIA
CCCCCCCCCCCHHCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA