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The map label for this gene is pgk

Identifier: 213157104

GI number: 213157104

Start: 1874444

End: 1875631

Strand: Direct

Name: pgk

Synonym: AB57_1787

Alternate gene names: 213157104

Gene position: 1874444-1875631 (Clockwise)

Preceding gene: 213157090

Following gene: 213157105

Centisome position: 46.28

GC content: 39.39

Gene sequence:

>1188_bases
ATGAATTTTCAGCGCATGACTGACCTTAATTTAGCAGGTAAACGTGTCCTTATTCGTGAAGATTTAAACGTTCCTGTTAA
AAATGGTGTGATTACCAGCGATGCTCGTTTACGTGCCGCACTTCCAACAATAAAAGCTGCTTTAGAAAAAGGCGCTGCTG
TAATGGTATTTTCACATTTAGGTCGTCCTGTGGAAGGTGAGCCAAAGCCTGAACAATCACTTGCACCAGTGGCTGCATAT
TTAACTGAAGCATTAGGTCAAGAAGTTAAATTATTTACTGATTATTTAGATGGTGTTGAAGTTGAAGCAGGACAAGTTGT
TTTGCTTGAAAATGTTCGTTTTAATCCGGGTGAAAAGAAAAATAATCCTGAACTTGCACAAAAATATGCAGCTCTATGTG
ATGTGTTTGTAATGGATGCTTTTGGTACTGCTCACCGTGCAGAAGCTTCTACAGAAGGTGTAGCACGTTTTGCACCTGTT
GCAGCGGCAGGCCCATTATTGGCTGCTGAATTAGATGCACTTGGCCGTGCAATGCAAACACCTGAAAAACCAATGGTGGC
AATTGTCGCTGGTTCTAAAGTTTCAACAAAACTTGATGTATTAAATTCATTATCTGGTATTTGTGACCAACTGATTGTGG
GAGGTGGTATTGCAAATACTTTCTTGGCGGCTGCTGGTTACAATGTTGGTAAATCTTTATATGAAGCTGACTTGGTTGAA
ACTGCAAAACAAATTGCTGCTAAAGTAAGTGTTCCACTTCCTACAGACGTAGTTGTAGCTGATGCCTCACAAATTAACTT
CGAAGATTTCTTAGGTTCTTTGGCAGCAGCGCAAGCAGTAATTAAGAAAGTTGAAGATGTCACAGCAAATGATATGATTT
TGGATGTGGGTCCTGAAACAGCTAAAGCATTTGCAAATATTTTAACAACTTCAAAAACGATTCTTTGGAATGGTCCTGTT
GGTGTATTTGAAGTTGATCAATTTGGTGAAGGTACTAAAGCACTTTCTCTTGCAGTTGCACAATCTGATGCATTCTCAAT
TGCTGGTGGTGGTGATACTTTAGCTGCGATCGATAAATATAATGTAGCTGATCAAATTGGTTATATTTCAACAGGTGGTG
GTGCATTCCTTGAATTTGTTGAAGGAAAAACATTGCCAGCAGTTGCCGTTTTACTTGAACGTGCTTAA

Upstream 100 bases:

>100_bases
AAAACTTTGGCTTTCTACAGCTTTTATGCAAAAAAACTAACCTTTTTTGATGTAATCATTTAAAATTGGCGCGTTTTTAA
AATCATAAAGAGAGCTTTAC

Downstream 100 bases:

>100_bases
AAACTGTTATATTGAAATAAGAAGGGATTGAGTTATCAATCCCTTTTTCATTGATAAGTCATTAAATTGTCATATTCCTG
AAATAATATAATGAGTACTA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNFQRMTDLNLAGKRVLIREDLNVPVKNGVITSDARLRAALPTIKAALEKGAAVMVFSHLGRPVEGEPKPEQSLAPVAAY
LTEALGQEVKLFTDYLDGVEVEAGQVVLLENVRFNPGEKKNNPELAQKYAALCDVFVMDAFGTAHRAEASTEGVARFAPV
AAAGPLLAAELDALGRAMQTPEKPMVAIVAGSKVSTKLDVLNSLSGICDQLIVGGGIANTFLAAAGYNVGKSLYEADLVE
TAKQIAAKVSVPLPTDVVVADASQINFEDFLGSLAAAQAVIKKVEDVTANDMILDVGPETAKAFANILTTSKTILWNGPV
GVFEVDQFGEGTKALSLAVAQSDAFSIAGGGDTLAAIDKYNVADQIGYISTGGGAFLEFVEGKTLPAVAVLLERA

Sequences:

>Translated_395_residues
MNFQRMTDLNLAGKRVLIREDLNVPVKNGVITSDARLRAALPTIKAALEKGAAVMVFSHLGRPVEGEPKPEQSLAPVAAY
LTEALGQEVKLFTDYLDGVEVEAGQVVLLENVRFNPGEKKNNPELAQKYAALCDVFVMDAFGTAHRAEASTEGVARFAPV
AAAGPLLAAELDALGRAMQTPEKPMVAIVAGSKVSTKLDVLNSLSGICDQLIVGGGIANTFLAAAGYNVGKSLYEADLVE
TAKQIAAKVSVPLPTDVVVADASQINFEDFLGSLAAAQAVIKKVEDVTANDMILDVGPETAKAFANILTTSKTILWNGPV
GVFEVDQFGEGTKALSLAVAQSDAFSIAGGGDTLAAIDKYNVADQIGYISTGGGAFLEFVEGKTLPAVAVLLERA
>Mature_395_residues
MNFQRMTDLNLAGKRVLIREDLNVPVKNGVITSDARLRAALPTIKAALEKGAAVMVFSHLGRPVEGEPKPEQSLAPVAAY
LTEALGQEVKLFTDYLDGVEVEAGQVVLLENVRFNPGEKKNNPELAQKYAALCDVFVMDAFGTAHRAEASTEGVARFAPV
AAAGPLLAAELDALGRAMQTPEKPMVAIVAGSKVSTKLDVLNSLSGICDQLIVGGGIANTFLAAAGYNVGKSLYEADLVE
TAKQIAAKVSVPLPTDVVVADASQINFEDFLGSLAAAQAVIKKVEDVTANDMILDVGPETAKAFANILTTSKTILWNGPV
GVFEVDQFGEGTKALSLAVAQSDAFSIAGGGDTLAAIDKYNVADQIGYISTGGGAFLEFVEGKTLPAVAVLLERA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=419, Percent_Identity=39.8568019093079, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI31543397, Length=419, Percent_Identity=38.1861575178998, Blast_Score=259, Evalue=3e-69,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=64.5569620253165, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17508823, Length=425, Percent_Identity=42.3529411764706, Blast_Score=275, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6319857, Length=422, Percent_Identity=41.9431279620853, Blast_Score=260, Evalue=2e-70,
Organism=Drosophila melanogaster, GI17136394, Length=427, Percent_Identity=41.2177985948478, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24581203, Length=418, Percent_Identity=38.755980861244, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ACIB3 (B7H3N8)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002325836.1
- ProteinModelPortal:   B7H3N8
- SMR:   B7H3N8
- GeneID:   7057658
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_001933
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41276; Mature: 41276

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 113-113 BINDING 146-146 BINDING 197-197 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQRMTDLNLAGKRVLIREDLNVPVKNGVITSDARLRAALPTIKAALEKGAAVMVFSHL
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEHHHC
GRPVEGEPKPEQSLAPVAAYLTEALGQEVKLFTDYLDGVEVEAGQVVLLENVRFNPGEKK
CCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCC
NNPELAQKYAALCDVFVMDAFGTAHRAEASTEGVARFAPVAAAGPLLAAELDALGRAMQT
CCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHCC
PEKPMVAIVAGSKVSTKLDVLNSLSGICDQLIVGGGIANTFLAAAGYNVGKSLYEADLVE
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHH
TAKQIAAKVSVPLPTDVVVADASQINFEDFLGSLAAAQAVIKKVEDVTANDMILDVGPET
HHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHH
AKAFANILTTSKTILWNGPVGVFEVDQFGEGTKALSLAVAQSDAFSIAGGGDTLAAIDKY
HHHHHHHHHCCCEEEECCCCCEEEECCCCCCCHHEEEEEECCCCEEEECCCCCEEHHHCC
NVADQIGYISTGGGAFLEFVEGKTLPAVAVLLERA
CHHHHCCEEECCCHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNFQRMTDLNLAGKRVLIREDLNVPVKNGVITSDARLRAALPTIKAALEKGAAVMVFSHL
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEHHHC
GRPVEGEPKPEQSLAPVAAYLTEALGQEVKLFTDYLDGVEVEAGQVVLLENVRFNPGEKK
CCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCC
NNPELAQKYAALCDVFVMDAFGTAHRAEASTEGVARFAPVAAAGPLLAAELDALGRAMQT
CCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHCC
PEKPMVAIVAGSKVSTKLDVLNSLSGICDQLIVGGGIANTFLAAAGYNVGKSLYEADLVE
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHH
TAKQIAAKVSVPLPTDVVVADASQINFEDFLGSLAAAQAVIKKVEDVTANDMILDVGPET
HHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHH
AKAFANILTTSKTILWNGPVGVFEVDQFGEGTKALSLAVAQSDAFSIAGGGDTLAAIDKY
HHHHHHHHHCCCEEEECCCCCEEEECCCCCCCHHEEEEEECCCCEEEECCCCCEEHHHCC
NVADQIGYISTGGGAFLEFVEGKTLPAVAVLLERA
CHHHHCCEEECCCHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA