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The map label for this gene is pleD [H]

Identifier: 213156954

GI number: 213156954

Start: 1719268

End: 1720227

Strand: Direct

Name: pleD [H]

Synonym: AB57_1629

Alternate gene names: 213156954

Gene position: 1719268-1720227 (Clockwise)

Preceding gene: 213156952

Following gene: 213156955

Centisome position: 42.45

GC content: 40.42

Gene sequence:

>960_bases
ATGAGTATGCAACATGTTTCTTTTCAGCAAGCTTCGCCAAACGAGCAGGCTGTTATTCTGATTGTGGATGATGTGCCGGA
AAATTTAGGCTTGCTACATGAAAGTCTGGATCAGGCAGGTTATCGCGTGCTTGTCACAACCGATGGGTTAAGTGCAATTG
AAATTGCACACCGATGCTTACCGGATATGATTTTGCTTGATGGCAATATGCCGCATATGGACGGTTTTGAAAGCTGCATA
CAGCTTAAAGCGAGTCCAATTACGCAGTTTATTCCCGTTATTTTTATGACAGGTTTGTCTGAGACAGAGCATATTGTGCG
TGGTTTTCAGGTCGGCGGGGTGGACTACGTAACTAAGCCACTGAATATTGAAGAAGTATTAGCAAGAGTAAAAACACATT
TGGCACATGCCAAACTATTGCAACAACAGAAACAGGTGATTGATGCAACGGAAACAGCCATTCTTGCGCTAGATGTTCAC
GGTAAAATTGTTTGGAAAACTCAAAAAGCCAGTGATTTACTGAATCAATATTTACCTGAATTAGAAGGTTTTGAAGCAGG
CTTAAAAGCTTGGTTTTCAGATTTGGAACATGAAAAAGACTCCAAAAAATTAACCAGCTGTTTTTATTCAACACCATCTC
AGCAATTACAGCTTTTATTGCTTACGCCGTGGGAAACTGAACCAAGTGTGGCAAAAAATTATCTTATCCAGATAAAAACT
TCTACGCCAGCTTTGGAAATGGCTGATATTTTGAAATATTGCCCACAGTTGACTCAACGTGAGGCAGAAGTCATGCACTG
GTTGTTGTTAGGGAAAACCAATAAGGATATTGCAGAAATTTTGGAGCTGAGTCCACGGACAGTGAATAAACATTTAGAGC
ATGTTTTTGAAAAACTTTGCGTTGAAAACCGAACTGCTGCGGTGGCGCATATTAATAGCTTGGTGCAAGAGTTTCACTAA

Upstream 100 bases:

>100_bases
GGCAAAATTTTCCGGAGCATGATGAGTTATGGGAACAGCTGGAACAGGCTATTCGAAAGTTTGATTTAAAACACGCGCAA
CGATTGATTCAGGATAAGCA

Downstream 100 bases:

>100_bases
TTTAAGTGAAGATTGTATTTAAAAAGCATACAGACTCACTGCTAAAAATAGGCTATTTTTTTACAGTTAAATTTGTTCTT
ATAGATAAAGATTATACAAA

Product: two component transcriptional regulator, LuxR family

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSMQHVSFQQASPNEQAVILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCLPDMILLDGNMPHMDGFESCI
QLKASPITQFIPVIFMTGLSETEHIVRGFQVGGVDYVTKPLNIEEVLARVKTHLAHAKLLQQQKQVIDATETAILALDVH
GKIVWKTQKASDLLNQYLPELEGFEAGLKAWFSDLEHEKDSKKLTSCFYSTPSQQLQLLLLTPWETEPSVAKNYLIQIKT
STPALEMADILKYCPQLTQREAEVMHWLLLGKTNKDIAEILELSPRTVNKHLEHVFEKLCVENRTAAVAHINSLVQEFH

Sequences:

>Translated_319_residues
MSMQHVSFQQASPNEQAVILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCLPDMILLDGNMPHMDGFESCI
QLKASPITQFIPVIFMTGLSETEHIVRGFQVGGVDYVTKPLNIEEVLARVKTHLAHAKLLQQQKQVIDATETAILALDVH
GKIVWKTQKASDLLNQYLPELEGFEAGLKAWFSDLEHEKDSKKLTSCFYSTPSQQLQLLLLTPWETEPSVAKNYLIQIKT
STPALEMADILKYCPQLTQREAEVMHWLLLGKTNKDIAEILELSPRTVNKHLEHVFEKLCVENRTAAVAHINSLVQEFH
>Mature_318_residues
SMQHVSFQQASPNEQAVILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCLPDMILLDGNMPHMDGFESCIQ
LKASPITQFIPVIFMTGLSETEHIVRGFQVGGVDYVTKPLNIEEVLARVKTHLAHAKLLQQQKQVIDATETAILALDVHG
KIVWKTQKASDLLNQYLPELEGFEAGLKAWFSDLEHEKDSKKLTSCFYSTPSQQLQLLLLTPWETEPSVAKNYLIQIKTS
TPALEMADILKYCPQLTQREAEVMHWLLLGKTNKDIAEILELSPRTVNKHLEHVFEKLCVENRTAAVAHINSLVQEFH

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=112, Percent_Identity=38.3928571428571, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786599, Length=144, Percent_Identity=31.25, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786784, Length=132, Percent_Identity=32.5757575757576, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790437, Length=138, Percent_Identity=29.7101449275362, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=31.0606060606061, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787229, Length=126, Percent_Identity=34.9206349206349, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788394, Length=128, Percent_Identity=35.15625, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=31.4814814814815, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=31.8965517241379, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI145693157, Length=116, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35878; Mature: 35747

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMQHVSFQQASPNEQAVILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCL
CCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHC
PDMILLDGNMPHMDGFESCIQLKASPITQFIPVIFMTGLSETEHIVRGFQVGGVDYVTKP
CCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCC
LNIEEVLARVKTHLAHAKLLQQQKQVIDATETAILALDVHGKIVWKTQKASDLLNQYLPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEECHHHHHHHHHHHHH
LEGFEAGLKAWFSDLEHEKDSKKLTSCFYSTPSQQLQLLLLTPWETEPSVAKNYLIQIKT
HCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHEEEEEECCCCCCHHHHCCEEEEEEC
STPALEMADILKYCPQLTQREAEVMHWLLLGKTNKDIAEILELSPRTVNKHLEHVFEKLC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
VENRTAAVAHINSLVQEFH
HCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SMQHVSFQQASPNEQAVILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCL
CCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHC
PDMILLDGNMPHMDGFESCIQLKASPITQFIPVIFMTGLSETEHIVRGFQVGGVDYVTKP
CCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCC
LNIEEVLARVKTHLAHAKLLQQQKQVIDATETAILALDVHGKIVWKTQKASDLLNQYLPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEECHHHHHHHHHHHHH
LEGFEAGLKAWFSDLEHEKDSKKLTSCFYSTPSQQLQLLLLTPWETEPSVAKNYLIQIKT
HCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHEEEEEECCCCCCHHHHCCEEEEEEC
STPALEMADILKYCPQLTQREAEVMHWLLLGKTNKDIAEILELSPRTVNKHLEHVFEKLC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
VENRTAAVAHINSLVQEFH
HCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA