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The map label for this gene is rsmB [H]

Identifier: 212225119

GI number: 212225119

Start: 1845509

End: 1846861

Strand: Reverse

Name: rsmB [H]

Synonym: TON_1966

Alternate gene names: 212225119

Gene position: 1846861-1845509 (Counterclockwise)

Preceding gene: NA

Following gene: 212225117

Centisome position: 99.96

GC content: 53.14

Gene sequence:

>1353_bases
ATGGAGCTGTTTTACAGAGTGAGCTTTCAGGAAGTAGTGGCCGACGCCCTCATGCTCGTTGAGGAGCGCGAGCTGTCTTC
GAAGCACGCCCTCGAGAGGGTCTTTAAGAAGGTCGCCGGTAAAGACCGCGAGAAGGCTCGCGGATTGGCCCACGCCTACG
TCTTCGAGATAGAGAAGTGGCGGGCCAAGATAGACTTCATAATCAACTCCGTTCTCAAAGGCTCGACGGTTGAGGACCTC
GACCCATACCTGGCAAACCTCCTCCGCATAGGGACGTTTGAGATCCACTTCAGAAAGGTTCCGCCGGCGATAGCGACCGA
CTCTATAATCCGCGTCGTCAAGGGGCACTTTGATTTTTCCCGCGCCAAGTTTGTTAACGCGCTGATGCACGAGATAGAGA
AGTTCGACGTCGAGAGGGCCCTAAAGAGGCTCAAAGAGAGGGACAGAATCGAGTGGCTCTCCGTCCGCTTCTCGCACCCT
AGGTGGTATGTGGAGTATGCAGTCGAGCTCTTCGGCTACGATGAGGCCGTTCGCCTGCTCCTCAGCAACAACAAGGCGCA
GCGCTACTATGTGAGGGCTAACACCCTAAAGACGGACGTTGATTCCCTGAGGGATTATCTCGAAGAGAACGGCGTGAGAA
CTGCCTTAACTCCCCTTCCCGACGTGCTCAAGATTCTCGAGTATAAAACGCCCGTAACAAGGCTCAACTGGTACAAACAA
GGCAAGTTCGTAATTCAGGATTTGGCGAGTGCCTACGTCGCCCACGTTTTAGCTCCAGAGCCTGGGGAGAGGGTTCTCGA
CCTGGCGGCAGCACCGGGGAGCAAGACCTTCCACGCAGCGGCGCTAATGGAGAACAAAGGGGAGATTGTAGCAGTTGACT
ACTCCTACGACAGGCTTATGCGCATGAAGGAGAAGATGAAATTGCTCGGAATAAAAAACGTCAAGCTCGTCCACGCGGAC
GGCCAGAGCTTCAGGGATAAAGAGAAATTTGATAAAATAATCCTCGACGCCCCATGTTCAAGCTCGGGAACCTACAGGCA
GTTTCCGGAAGTCAAGTGGCGCTTCGACGAGAAGAAGATCAAGCGCATCATAAACGTCCAGAGAAACATGTTGAGGAATG
CCCATGAGAACCTGAGAGACGGCGGCGAGATGACGTATTCGACGTGCTCTATAAGGATTGACGAGGACGAGGAAAACGTT
CTCTTCGCAATAAACCGGGTCGGGCTTGAGCTTATCGATTATCCCTTCAGCTGGGGCGATAGGGGCTTCCTTGAAATAGG
GGACAAGGTTTTCAGGGCATGGACGCACAGGCACGACTGCAACAGCTTCTTCATTGCGAAGTTGAAAAAATAA

Upstream 100 bases:

>100_bases
CCCACCACAATTAACCCCACCTTTAGAGGTAATAAAGGTTTTGGGACTTCAAGTAGAACTGCACCACCACCTTTTTAAAC
TTCCCTGCGAAGGCTCGGCC

Downstream 100 bases:

>100_bases
TCACACCAGCGGCTTTCTCTTCACGGGCCCCTTGGGATACTCCTCTTCAAATATCTCCGCTGTGAAGCGGTAGACTTTAG
TGTCCTCGTCAAGCCAGCAG

Product: tRNA/rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MELFYRVSFQEVVADALMLVEERELSSKHALERVFKKVAGKDREKARGLAHAYVFEIEKWRAKIDFIINSVLKGSTVEDL
DPYLANLLRIGTFEIHFRKVPPAIATDSIIRVVKGHFDFSRAKFVNALMHEIEKFDVERALKRLKERDRIEWLSVRFSHP
RWYVEYAVELFGYDEAVRLLLSNNKAQRYYVRANTLKTDVDSLRDYLEENGVRTALTPLPDVLKILEYKTPVTRLNWYKQ
GKFVIQDLASAYVAHVLAPEPGERVLDLAAAPGSKTFHAAALMENKGEIVAVDYSYDRLMRMKEKMKLLGIKNVKLVHAD
GQSFRDKEKFDKIILDAPCSSSGTYRQFPEVKWRFDEKKIKRIINVQRNMLRNAHENLRDGGEMTYSTCSIRIDEDEENV
LFAINRVGLELIDYPFSWGDRGFLEIGDKVFRAWTHRHDCNSFFIAKLKK

Sequences:

>Translated_450_residues
MELFYRVSFQEVVADALMLVEERELSSKHALERVFKKVAGKDREKARGLAHAYVFEIEKWRAKIDFIINSVLKGSTVEDL
DPYLANLLRIGTFEIHFRKVPPAIATDSIIRVVKGHFDFSRAKFVNALMHEIEKFDVERALKRLKERDRIEWLSVRFSHP
RWYVEYAVELFGYDEAVRLLLSNNKAQRYYVRANTLKTDVDSLRDYLEENGVRTALTPLPDVLKILEYKTPVTRLNWYKQ
GKFVIQDLASAYVAHVLAPEPGERVLDLAAAPGSKTFHAAALMENKGEIVAVDYSYDRLMRMKEKMKLLGIKNVKLVHAD
GQSFRDKEKFDKIILDAPCSSSGTYRQFPEVKWRFDEKKIKRIINVQRNMLRNAHENLRDGGEMTYSTCSIRIDEDEENV
LFAINRVGLELIDYPFSWGDRGFLEIGDKVFRAWTHRHDCNSFFIAKLKK
>Mature_450_residues
MELFYRVSFQEVVADALMLVEERELSSKHALERVFKKVAGKDREKARGLAHAYVFEIEKWRAKIDFIINSVLKGSTVEDL
DPYLANLLRIGTFEIHFRKVPPAIATDSIIRVVKGHFDFSRAKFVNALMHEIEKFDVERALKRLKERDRIEWLSVRFSHP
RWYVEYAVELFGYDEAVRLLLSNNKAQRYYVRANTLKTDVDSLRDYLEENGVRTALTPLPDVLKILEYKTPVTRLNWYKQ
GKFVIQDLASAYVAHVLAPEPGERVLDLAAAPGSKTFHAAALMENKGEIVAVDYSYDRLMRMKEKMKLLGIKNVKLVHAD
GQSFRDKEKFDKIILDAPCSSSGTYRQFPEVKWRFDEKKIKRIINVQRNMLRNAHENLRDGGEMTYSTCSIRIDEDEENV
LFAINRVGLELIDYPFSWGDRGFLEIGDKVFRAWTHRHDCNSFFIAKLKK

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=333, Percent_Identity=31.8318318318318, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI76150623, Length=333, Percent_Identity=31.8318318318318, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI32698918, Length=176, Percent_Identity=36.9318181818182, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI8922322, Length=289, Percent_Identity=28.3737024221453, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI270288818, Length=289, Percent_Identity=28.3737024221453, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI270288816, Length=289, Percent_Identity=28.3737024221453, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI23199998, Length=289, Percent_Identity=28.3737024221453, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI11545785, Length=323, Percent_Identity=26.9349845201238, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI301336155, Length=240, Percent_Identity=26.25, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI39995082, Length=194, Percent_Identity=27.8350515463918, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI2367212, Length=408, Percent_Identity=25.4901960784314, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87081985, Length=291, Percent_Identity=32.3024054982818, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17536757, Length=294, Percent_Identity=32.6530612244898, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71998419, Length=244, Percent_Identity=32.3770491803279, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI115534021, Length=244, Percent_Identity=32.3770491803279, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25143471, Length=188, Percent_Identity=30.8510638297872, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=312, Percent_Identity=30.7692307692308, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324306, Length=244, Percent_Identity=27.0491803278689, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319447, Length=185, Percent_Identity=31.3513513513514, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI22024126, Length=332, Percent_Identity=30.1204819277108, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24668781, Length=238, Percent_Identity=34.453781512605, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356513, Length=238, Percent_Identity=34.453781512605, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356579, Length=220, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355201, Length=195, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 52411; Mature: 52411

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFYRVSFQEVVADALMLVEERELSSKHALERVFKKVAGKDREKARGLAHAYVFEIEKW
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RAKIDFIINSVLKGSTVEDLDPYLANLLRIGTFEIHFRKVPPAIATDSIIRVVKGHFDFS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCHH
RAKFVNALMHEIEKFDVERALKRLKERDRIEWLSVRFSHPRWYVEYAVELFGYDEAVRLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCHHHHHHHH
LSNNKAQRYYVRANTLKTDVDSLRDYLEENGVRTALTPLPDVLKILEYKTPVTRLNWYKQ
HCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHC
GKFVIQDLASAYVAHVLAPEPGERVLDLAAAPGSKTFHAAALMENKGEIVAVDYSYDRLM
CHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCCCCHHHHHHHCCCCCEEEEECCHHHHH
RMKEKMKLLGIKNVKLVHADGQSFRDKEKFDKIILDAPCSSSGTYRQFPEVKWRFDEKKI
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHEEEECCCCCCCCCCCCCCCCEECCHHHH
KRIINVQRNMLRNAHENLRDGGEMTYSTCSIRIDEDEENVLFAINRVGLELIDYPFSWGD
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEEEHHCHHHCCCCCCCCC
RGFLEIGDKVFRAWTHRHDCNSFFIAKLKK
CCHHHHHHHHHHHHHHCCCCCCCHHEEECC
>Mature Secondary Structure
MELFYRVSFQEVVADALMLVEERELSSKHALERVFKKVAGKDREKARGLAHAYVFEIEKW
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RAKIDFIINSVLKGSTVEDLDPYLANLLRIGTFEIHFRKVPPAIATDSIIRVVKGHFDFS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCHH
RAKFVNALMHEIEKFDVERALKRLKERDRIEWLSVRFSHPRWYVEYAVELFGYDEAVRLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCHHHHHHHH
LSNNKAQRYYVRANTLKTDVDSLRDYLEENGVRTALTPLPDVLKILEYKTPVTRLNWYKQ
HCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHC
GKFVIQDLASAYVAHVLAPEPGERVLDLAAAPGSKTFHAAALMENKGEIVAVDYSYDRLM
CHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCCCCHHHHHHHCCCCCEEEEECCHHHHH
RMKEKMKLLGIKNVKLVHADGQSFRDKEKFDKIILDAPCSSSGTYRQFPEVKWRFDEKKI
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHEEEECCCCCCCCCCCCCCCCEECCHHHH
KRIINVQRNMLRNAHENLRDGGEMTYSTCSIRIDEDEENVLFAINRVGLELIDYPFSWGD
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEEEHHCHHHCCCCCCCCC
RGFLEIGDKVFRAWTHRHDCNSFFIAKLKK
CCHHHHHHHHHHHHHHCCCCCCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]