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The map label for this gene is rpa [H]

Identifier: 212225107

GI number: 212225107

Start: 1832608

End: 1833672

Strand: Reverse

Name: rpa [H]

Synonym: TON_1954

Alternate gene names: 212225107

Gene position: 1833672-1832608 (Counterclockwise)

Preceding gene: 212225108

Following gene: 212225106

Centisome position: 99.25

GC content: 55.49

Gene sequence:

>1065_bases
ATGGTAGTGCTGACAAAGGAGCAGATCATCGAGATGATCGAGAGGCAGAAGGGCCTCTCGAGGAATGAAATTGAGGAGAG
GATAGCGGAGATAGCATCCCGCGAGGGTATTTCCGAGCACGCGGCAGCTTTAATGCTCGCGGAGGAACTGGGCGTTAACC
TGGAGGGCAAGGAGGAACTTCTCCACATAGCGGACCTTGTCCCCGGAATGGCCAACGTCAACATAGTCGCGAGGATACTG
AGGAAGTACCCGCCGAGGGAGTACCAGAAGAGGGACGGCTCAACCGGCCAGGTTGCAAACGTGATAATCTACGACTCAAC
CGGAAAGACAAGGCTCGTTCTCTGGGACGCTCAGGTGGCAAAGTACTACAACGAGCTGAACCCGGGCGACATCATCAAGA
TCATCGATCCGAGCGTTAGAGAGGGAAGGAGCGGAGTTGAGCTCCACGCCAACTTCAGGACAAGGATAATACTCAATCCG
GAAGACCCGCGTGTCGAGGAGATACCACCGCTGGAGGAAGTGAGAAGCTACAACTACAGAAGAACAAAGATAGGCGAGCT
CATGGGCGGCGAGCGCTTTGTTGAGGTCAGGGGAACGATAGCGAGGCTTTACAGGGTTACCGTTTACGACGCCTGCCCTG
AGTGCAGGAGGAAAGTTGATTACGAGCCGGCAACAGATACGTGGATATGCCCCGAGCACGGCGAGATCCAGCCGATAAAG
ATAACCATCATCGACTTTGGCCTCGACGACTCAACTGGTTACATAAGGACGACGCTCTTCGGCGACGATGCGGCCGAGCT
TCTCGGAAAAGACCCAGAGGAGATAGCAGAAAAGCTCAGGGAGCTGGTGGAGGACGGACTGACCCTCAAGGAAGCCAGCA
GAAAGCTCGCGGAGGAGGAGTACTACTACCTGCTCGGAAGGGAAATAGTCGTCAGGGGCAACGTCGTGGACGACAAGTTC
CTTGGGCTAATCCTCAAGGCCTTTGGCTGGGACGAGGTGGACTACAAGAGAGAGATAAGCCGCGTCAGGGCCGAGCTGAA
GGAGGTCACTAAGGAGGTGCTGTGA

Upstream 100 bases:

>100_bases
TTTCTGTTCTTGGGAGGAATACGGAAGCTTTTTCCGGAAACTCGGCCTCCGGAGAAAAGTTTAAATACCACCACCCTGTA
TTACCATTAAGGTGAGTGTT

Downstream 100 bases:

>100_bases
ATGGAGGAGGTCAGGTACAGGCGGCGCAAGCCCGCCGTCGAGAGGAAGATAGGCGAGATAAGGGAAGACGACACGAGAGT
TGCGCTTATAGGCAAGGCTT

Product: replication factor A

Products: NA

Alternate protein names: RF-A; RP-A; Replication factor A protein 1; Single-stranded DNA-binding protein; mjaSSB [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MVVLTKEQIIEMIERQKGLSRNEIEERIAEIASREGISEHAAALMLAEELGVNLEGKEELLHIADLVPGMANVNIVARIL
RKYPPREYQKRDGSTGQVANVIIYDSTGKTRLVLWDAQVAKYYNELNPGDIIKIIDPSVREGRSGVELHANFRTRIILNP
EDPRVEEIPPLEEVRSYNYRRTKIGELMGGERFVEVRGTIARLYRVTVYDACPECRRKVDYEPATDTWICPEHGEIQPIK
ITIIDFGLDDSTGYIRTTLFGDDAAELLGKDPEEIAEKLRELVEDGLTLKEASRKLAEEEYYYLLGREIVVRGNVVDDKF
LGLILKAFGWDEVDYKREISRVRAELKEVTKEVL

Sequences:

>Translated_354_residues
MVVLTKEQIIEMIERQKGLSRNEIEERIAEIASREGISEHAAALMLAEELGVNLEGKEELLHIADLVPGMANVNIVARIL
RKYPPREYQKRDGSTGQVANVIIYDSTGKTRLVLWDAQVAKYYNELNPGDIIKIIDPSVREGRSGVELHANFRTRIILNP
EDPRVEEIPPLEEVRSYNYRRTKIGELMGGERFVEVRGTIARLYRVTVYDACPECRRKVDYEPATDTWICPEHGEIQPIK
ITIIDFGLDDSTGYIRTTLFGDDAAELLGKDPEEIAEKLRELVEDGLTLKEASRKLAEEEYYYLLGREIVVRGNVVDDKF
LGLILKAFGWDEVDYKREISRVRAELKEVTKEVL
>Mature_354_residues
MVVLTKEQIIEMIERQKGLSRNEIEERIAEIASREGISEHAAALMLAEELGVNLEGKEELLHIADLVPGMANVNIVARIL
RKYPPREYQKRDGSTGQVANVIIYDSTGKTRLVLWDAQVAKYYNELNPGDIIKIIDPSVREGRSGVELHANFRTRIILNP
EDPRVEEIPPLEEVRSYNYRRTKIGELMGGERFVEVRGTIARLYRVTVYDACPECRRKVDYEPATDTWICPEHGEIQPIK
ITIIDFGLDDSTGYIRTTLFGDDAAELLGKDPEEIAEKLRELVEDGLTLKEASRKLAEEEYYYLLGREIVVRGNVVDDKF
LGLILKAFGWDEVDYKREISRVRAELKEVTKEVL

Specific function: Probably plays an essential for replication of the chromosome, DNA recombination and repair [H]

COG id: COG1599

COG function: function code L; Single-stranded DNA-binding replication protein A (RPA), large (70 kD) subunit and related ssDNA-binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 OB DNA-binding domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR013955 [H]

Pfam domain/function: PF08646 Rep_fac-A_C; PF01336 tRNA_anti [H]

EC number: NA

Molecular weight: Translated: 40448; Mature: 40448

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVLTKEQIIEMIERQKGLSRNEIEERIAEIASREGISEHAAALMLAEELGVNLEGKEEL
CEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHH
LHIADLVPGMANVNIVARILRKYPPREYQKRDGSTGQVANVIIYDSTGKTRLVLWDAQVA
HHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCCCEEEEEECHHHH
KYYNELNPGDIIKIIDPSVREGRSGVELHANFRTRIILNPEDPRVEEIPPLEEVRSYNYR
HHHHCCCCCCEEEEECCHHHCCCCCCEEEECCEEEEEECCCCCCCCCCCCHHHHHCCCCC
RTKIGELMGGERFVEVRGTIARLYRVTVYDACPECRRKVDYEPATDTWICPEHGEIQPIK
HHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEECCCCCCEEEEE
ITIIDFGLDDSTGYIRTTLFGDDAAELLGKDPEEIAEKLRELVEDGLTLKEASRKLAEEE
EEEEEECCCCCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YYYLLGREIVVRGNVVDDKFLGLILKAFGWDEVDYKREISRVRAELKEVTKEVL
HHHEECCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVLTKEQIIEMIERQKGLSRNEIEERIAEIASREGISEHAAALMLAEELGVNLEGKEEL
CEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHH
LHIADLVPGMANVNIVARILRKYPPREYQKRDGSTGQVANVIIYDSTGKTRLVLWDAQVA
HHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCCCEEEEEECHHHH
KYYNELNPGDIIKIIDPSVREGRSGVELHANFRTRIILNPEDPRVEEIPPLEEVRSYNYR
HHHHCCCCCCEEEEECCHHHCCCCCCEEEECCEEEEEECCCCCCCCCCCCHHHHHCCCCC
RTKIGELMGGERFVEVRGTIARLYRVTVYDACPECRRKVDYEPATDTWICPEHGEIQPIK
HHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEECCCCCCEEEEE
ITIIDFGLDDSTGYIRTTLFGDDAAELLGKDPEEIAEKLRELVEDGLTLKEASRKLAEEE
EEEEEECCCCCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YYYLLGREIVVRGNVVDDKFLGLILKAFGWDEVDYKREISRVRAELKEVTKEVL
HHHEECCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 9843941 [H]