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The map label for this gene is nplT [H]

Identifier: 212224947

GI number: 212224947

Start: 1666089

End: 1668047

Strand: Reverse

Name: nplT [H]

Synonym: TON_1796

Alternate gene names: 212224947

Gene position: 1668047-1666089 (Counterclockwise)

Preceding gene: 212224950

Following gene: 212224946

Centisome position: 90.28

GC content: 53.19

Gene sequence:

>1959_bases
GTGTATAAAACTTTCGGATTCGAGTCAAACGAATACTTTGGCCGGATCGCAAAGGTTGAATTTTCAGTCCCGTCCAGGGG
AAGCTACGCCTACCTCGTCGGGAGCTTCAACGCCTTCAACGAGGGCAGCTTCCGGATGAGGGAAGAGAACGGAAGATGGC
GTGCCACTGTTGAACTCCCCGAGGGGGTCTGGCACTACGGCTTCTCAATTGACGGAAAATACGCTCCAGACCCAGAGAAT
CCAGAGAAGAGGGCTTACAGGCGGTTCTCATACAAATTCGAGAGGGAAACCAGCGTTGCCAGAATATCCGCCGGAGACGA
CTTCTATCACGAACCCGCGCTCGCTTACTTGTACTCCTTTGCCGACAGAACCCATGTCCTGCTGAGGACTGTGAAGGGAA
AAGCAATCTCCACTTACCTCATCACCGATGAGCGTATAGAGATGAGAAAGAAGGCAAGCGACGAGCTCTTCGACTACTTC
GAGGCCGTTCTTCCAAGAACCGAGGAGCTGAGCTACGGCTTCGAGATAGAGACCGGAGAAGGAACAATCGAATACGGTAA
CTTCACCGCCGAACCGAGAGAGCTCCAAGTCCCAAGATGGATATTCAATCGGGTATTCTACCAGATAATGCCCGATAGGT
TCGAAAGAGACATGATCAAAAAGCCAAGAGGAAGGATAATCGAAACCGGTCTGGGGCACCACGGTGGCGATTTAGCCGGA
ATCGTGAAGAGGCTCGGCCACCTTGAGGGGCTCGGTGTGAATGCACTCTATCTGACCCCAATCTTCGAGTCCATGACGTA
CCATGGCTACGACATAGTTGATTACTTCAAGGTCGCGGGGAAGTTCGGAGGTAACGAGGCCTTCGGAGAGCTCGCCCGGG
AGCTGAAAAGGAGGGACATAAAACTGATTCTCGACGGCGTCTTCCACCACACGAGCTTTTTCCACTCATACTTTCAGGAC
GTCGTGAAGAAGGGCGGAGAGAGCAGGTACCGGGACTTCTACAGAATCCTGAAGTTCCCGGTGGTGTCGAAGGACTTCCT
TCGGGTTCTCGACTCGAACGAACCGCCGGAGCGAAAGTACAAGGGGCTGAAGGAGCTCCATCAGAACTATGAGAACTTCT
TCTCGGTGTGGCTCATGCCCCGTCTGAACCACGACAATCCACAGGTTAGGGAGTTCATAGTCAGTGTAATGAAACACTGG
CTCGAAGAAGGTGCCGATGGCTGGCGACTGGATGTCGCCCACGGCGTCCCGCCAGAGCTGTGGCGCGAGGTCAGGGAAAG
AATGCCGGAAGACGCGTATCTGGTAGGAGAAGTGATGGACGACGCAAGACTCTGGCTCTTCGACAAGTTCCACGGAACGA
TGAACTATCCCCTGTACGAGGCCATTCTAAGGTTCTTCGTGCGTGGAGAGATAAGTGCCGAAGAGTTCCTCAACTGGCTC
GAGCTCCTGAGCACCTATTATGGGCCCGCTGAATACTCCATGTACAACTTCCTTGACAACCACGACGTTGAGCGCTTCCT
CGACCTCGTCGGTGACGAGCGGAGATACCTCTGCGCTCTCGCTTTCCTGATGACATATAAGGGGATTCCAGCGCTTTTCT
ACGGTGACGAAATAGGACTGAGGGGAATAGGTGCTTCTGGGATGGAAAGCTCAAGGACTCCAATGAAATGGGGGAAAGAG
ACGTGGAACACCAAGATACTCAGAGTGACAAAAGCGCTGATACGGCTGAGAAGGAAGAGCAAAGCCTTACAGCTGGGAGA
ATTCAGGCCACTGGAGTTCAAAGGTGGCCTGCTCCTCTACGAGAGAGTTTACCAGAACGAAGGAGTTCTGGTGGGGATAA
ACTACTCAGACGTTCCCACAGCCATTCAGATTCCTGAAGCTTACCGTCCTGCAGCGGATGGAGTGTCCTTCCTCAAGATG
AAACCCTGGTCCTTTGTCGCCCTCGCCTCAACCATCTGA

Upstream 100 bases:

>100_bases
GAGCTTTTCGATAATTTCCTCTTCCTTCATTTTCAGACCTCCAACTTTTTAAGTTCACTAGCATTAGTTTAATCATCACT
AGTGATACCTGGTGGGAAAG

Downstream 100 bases:

>100_bases
TGCCACGTTTTTTCACCAGCGTTCCGGGTTGTACACACAATGCAAACTTGAACAGGGAAGCGCAAGGTATATATATCACT
GACGATGATACTATAACACC

Product: cyclomaltodextrinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MYKTFGFESNEYFGRIAKVEFSVPSRGSYAYLVGSFNAFNEGSFRMREENGRWRATVELPEGVWHYGFSIDGKYAPDPEN
PEKRAYRRFSYKFERETSVARISAGDDFYHEPALAYLYSFADRTHVLLRTVKGKAISTYLITDERIEMRKKASDELFDYF
EAVLPRTEELSYGFEIETGEGTIEYGNFTAEPRELQVPRWIFNRVFYQIMPDRFERDMIKKPRGRIIETGLGHHGGDLAG
IVKRLGHLEGLGVNALYLTPIFESMTYHGYDIVDYFKVAGKFGGNEAFGELARELKRRDIKLILDGVFHHTSFFHSYFQD
VVKKGGESRYRDFYRILKFPVVSKDFLRVLDSNEPPERKYKGLKELHQNYENFFSVWLMPRLNHDNPQVREFIVSVMKHW
LEEGADGWRLDVAHGVPPELWREVRERMPEDAYLVGEVMDDARLWLFDKFHGTMNYPLYEAILRFFVRGEISAEEFLNWL
ELLSTYYGPAEYSMYNFLDNHDVERFLDLVGDERRYLCALAFLMTYKGIPALFYGDEIGLRGIGASGMESSRTPMKWGKE
TWNTKILRVTKALIRLRRKSKALQLGEFRPLEFKGGLLLYERVYQNEGVLVGINYSDVPTAIQIPEAYRPAADGVSFLKM
KPWSFVALASTI

Sequences:

>Translated_652_residues
MYKTFGFESNEYFGRIAKVEFSVPSRGSYAYLVGSFNAFNEGSFRMREENGRWRATVELPEGVWHYGFSIDGKYAPDPEN
PEKRAYRRFSYKFERETSVARISAGDDFYHEPALAYLYSFADRTHVLLRTVKGKAISTYLITDERIEMRKKASDELFDYF
EAVLPRTEELSYGFEIETGEGTIEYGNFTAEPRELQVPRWIFNRVFYQIMPDRFERDMIKKPRGRIIETGLGHHGGDLAG
IVKRLGHLEGLGVNALYLTPIFESMTYHGYDIVDYFKVAGKFGGNEAFGELARELKRRDIKLILDGVFHHTSFFHSYFQD
VVKKGGESRYRDFYRILKFPVVSKDFLRVLDSNEPPERKYKGLKELHQNYENFFSVWLMPRLNHDNPQVREFIVSVMKHW
LEEGADGWRLDVAHGVPPELWREVRERMPEDAYLVGEVMDDARLWLFDKFHGTMNYPLYEAILRFFVRGEISAEEFLNWL
ELLSTYYGPAEYSMYNFLDNHDVERFLDLVGDERRYLCALAFLMTYKGIPALFYGDEIGLRGIGASGMESSRTPMKWGKE
TWNTKILRVTKALIRLRRKSKALQLGEFRPLEFKGGLLLYERVYQNEGVLVGINYSDVPTAIQIPEAYRPAADGVSFLKM
KPWSFVALASTI
>Mature_652_residues
MYKTFGFESNEYFGRIAKVEFSVPSRGSYAYLVGSFNAFNEGSFRMREENGRWRATVELPEGVWHYGFSIDGKYAPDPEN
PEKRAYRRFSYKFERETSVARISAGDDFYHEPALAYLYSFADRTHVLLRTVKGKAISTYLITDERIEMRKKASDELFDYF
EAVLPRTEELSYGFEIETGEGTIEYGNFTAEPRELQVPRWIFNRVFYQIMPDRFERDMIKKPRGRIIETGLGHHGGDLAG
IVKRLGHLEGLGVNALYLTPIFESMTYHGYDIVDYFKVAGKFGGNEAFGELARELKRRDIKLILDGVFHHTSFFHSYFQD
VVKKGGESRYRDFYRILKFPVVSKDFLRVLDSNEPPERKYKGLKELHQNYENFFSVWLMPRLNHDNPQVREFIVSVMKHW
LEEGADGWRLDVAHGVPPELWREVRERMPEDAYLVGEVMDDARLWLFDKFHGTMNYPLYEAILRFFVRGEISAEEFLNWL
ELLSTYYGPAEYSMYNFLDNHDVERFLDLVGDERRYLCALAFLMTYKGIPALFYGDEIGLRGIGASGMESSRTPMKWGKE
TWNTKILRVTKALIRLRRKSKALQLGEFRPLEFKGGLLLYERVYQNEGVLVGINYSDVPTAIQIPEAYRPAADGVSFLKM
KPWSFVALASTI

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=474, Percent_Identity=29.957805907173, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1790687, Length=536, Percent_Identity=25.1865671641791, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322245, Length=241, Percent_Identity=27.8008298755187, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586599, Length=550, Percent_Identity=24.5454545454545, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI221330053, Length=216, Percent_Identity=34.2592592592593, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24586589, Length=514, Percent_Identity=25.6809338521401, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24586587, Length=223, Percent_Identity=34.0807174887892, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583749, Length=219, Percent_Identity=32.8767123287671, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24583747, Length=219, Percent_Identity=32.8767123287671, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586593, Length=543, Percent_Identity=23.0202578268877, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24586597, Length=503, Percent_Identity=26.0437375745527, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24586591, Length=221, Percent_Identity=30.316742081448, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583745, Length=224, Percent_Identity=30.3571428571429, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45549022, Length=211, Percent_Identity=30.3317535545024, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 75988; Mature: 75988

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKTFGFESNEYFGRIAKVEFSVPSRGSYAYLVGSFNAFNEGSFRMREENGRWRATVELP
CCCCCCCCCCHHHCEEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECC
EGVWHYGFSIDGKYAPDPENPEKRAYRRFSYKFERETSVARISAGDDFYHEPALAYLYSF
CCCEEECEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHCHHHHHHHHH
ADRTHVLLRTVKGKAISTYLITDERIEMRKKASDELFDYFEAVLPRTEELSYGFEIETGE
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
GTIEYGNFTAEPRELQVPRWIFNRVFYQIMPDRFERDMIKKPRGRIIETGLGHHGGDLAG
CEEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEECCCCCCCCHHHH
IVKRLGHLEGLGVNALYLTPIFESMTYHGYDIVDYFKVAGKFGGNEAFGELARELKRRDI
HHHHHHCCCCCCCCEEEECHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
KLILDGVFHHTSFFHSYFQDVVKKGGESRYRDFYRILKFPVVSKDFLRVLDSNEPPERKY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHH
KGLKELHQNYENFFSVWLMPRLNHDNPQVREFIVSVMKHWLEEGADGWRLDVAHGVPPEL
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
WREVRERMPEDAYLVGEVMDDARLWLFDKFHGTMNYPLYEAILRFFVRGEISAEEFLNWL
HHHHHHHCCCCCEEHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
ELLSTYYGPAEYSMYNFLDNHDVERFLDLVGDERRYLCALAFLMTYKGIPALFYGDEIGL
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
RGIGASGMESSRTPMKWGKETWNTKILRVTKALIRLRRKSKALQLGEFRPLEFKGGLLLY
CCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEE
ERVYQNEGVLVGINYSDVPTAIQIPEAYRPAADGVSFLKMKPWSFVALASTI
HHHHCCCCEEEEECCCCCCCEEECCHHCCCCCCCCCEEEECCCCEEEHHHCC
>Mature Secondary Structure
MYKTFGFESNEYFGRIAKVEFSVPSRGSYAYLVGSFNAFNEGSFRMREENGRWRATVELP
CCCCCCCCCCHHHCEEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECC
EGVWHYGFSIDGKYAPDPENPEKRAYRRFSYKFERETSVARISAGDDFYHEPALAYLYSF
CCCEEECEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHCHHHHHHHHH
ADRTHVLLRTVKGKAISTYLITDERIEMRKKASDELFDYFEAVLPRTEELSYGFEIETGE
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
GTIEYGNFTAEPRELQVPRWIFNRVFYQIMPDRFERDMIKKPRGRIIETGLGHHGGDLAG
CEEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEECCCCCCCCHHHH
IVKRLGHLEGLGVNALYLTPIFESMTYHGYDIVDYFKVAGKFGGNEAFGELARELKRRDI
HHHHHHCCCCCCCCEEEECHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
KLILDGVFHHTSFFHSYFQDVVKKGGESRYRDFYRILKFPVVSKDFLRVLDSNEPPERKY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHH
KGLKELHQNYENFFSVWLMPRLNHDNPQVREFIVSVMKHWLEEGADGWRLDVAHGVPPEL
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
WREVRERMPEDAYLVGEVMDDARLWLFDKFHGTMNYPLYEAILRFFVRGEISAEEFLNWL
HHHHHHHCCCCCEEHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
ELLSTYYGPAEYSMYNFLDNHDVERFLDLVGDERRYLCALAFLMTYKGIPALFYGDEIGL
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
RGIGASGMESSRTPMKWGKETWNTKILRVTKALIRLRRKSKALQLGEFRPLEFKGGLLLY
CCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEE
ERVYQNEGVLVGINYSDVPTAIQIPEAYRPAADGVSFLKMKPWSFVALASTI
HHHHCCCCEEEEECCCCCCCEEECCHHCCCCCCCCCEEEECCCCEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]