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The map label for this gene is ygcA [C]

Identifier: 212224662

GI number: 212224662

Start: 1386364

End: 1387623

Strand: Reverse

Name: ygcA [C]

Synonym: TON_1511

Alternate gene names: 212224662

Gene position: 1387623-1386364 (Counterclockwise)

Preceding gene: 212224664

Following gene: 212224661

Centisome position: 75.1

GC content: 48.81

Gene sequence:

>1260_bases
ATGAGGGGAGTCGTTGAGAGACTGGATTTGGAAGGACTGGGCATAGTCAGGCTGGATAAACGGGAAATTCACGTCCCATT
TACAGCCCCTGGCGATATCGTCGAGATTAAAAGATGGCGGAAAAAGAAGAGAACGCTCATAGCAACTGAGTATGAAGTAG
TTGGGCCTTCTGAGATTCGAACCGAACCAAAGTGTCCATATTTTGGAACCTGCGGCGGCTGTCTCTTACAGCACCTTCCC
TACGATGAGCAGCTGAAGTTCAAATCTGACAAGCTTTCGGAGATTCTTGGTTTTGATGTCGAGGTTATTCCTTCACCGAA
GATATATGGCCATAGAAATCGTATTGATGTTGTCATTTCGACAAATGGGATTGGCTTCAGAAGGAGGGGCACTTGGTGGG
ACGTCGTGGACATCGAGGAGTGTCCCGTCTTCGGAAAAGCCAGTGGAAAGGTTCTCCGTTCGCTCAGGGAGTTTATAGAG
GATCACAAGCCGAGCCTCTATGAGATAGGCAAAAACAGGGGCTTTTTGAGGTATGTAGTCATTCGTGAGGGCAAGTTCAC
GGATGAACTGATGGTGAACCTTGTAACTGCCGAGGGGGAACTTCCCGAGGAGTTCCCGGACTACTTCGACTACGCTGACT
CTATCTACTGGAGCGTGAACAGGACACCAAGCGATGTCTCCTATGGCGATGTGGAAAGGGTTTGGAAGAAAGAGTTCATA
CGCGAGAGGCTCGATGACGTTGCTTACCTTATCCACCCCAACAGCTTTTTCCAGACTAACAGCTACCAAGCGGTTAACTT
AGTCAGGGAAGTTTCCAGACTCGTGGAGGGTGAAAAAGTTCTCGATCTGTATTCTGGCGTCGGAACCTTCGGCGTTTACC
TTGCGAAGAGGGGCTTTACCGTTGAGGGCATCGAGATTAACCCCTTCGCGGTTGAGATGGCCAACAGAAATGCCGAACTA
AACGGGATTGATGCAGTCTTTTCGGTTGGAGAGGACAAAGACGTCAAGAACTTGGCGAAATACGACACGATAATCCTCGA
TCCGCCGAGAGCGGGCTTGCATCCAAAGTTGGTCAAGAAAATCTTAAAAGATGAACCGCAGACCATCGTTTACGTCTCCT
GCAACCCAAAGACCCTTAAACTGAACTTGAACGAGCTATCTGGAAAGTACTCTGTGGAAGAGGCAGTTGGTCTCGACATG
TTCCCTCACACTCCCCACATCGAAGTGGTTGTCAAACTTAAACACAAGGTTTGGCTTTAG

Upstream 100 bases:

>100_bases
ACCGTTATTTACTACTCTCTCCACCTAAATAAACCTTCCGAAACTACATTTGACACTCGTTCAAGGGCAGATTTATAAGG
CACCCTCTGAAGGAAGAATC

Downstream 100 bases:

>100_bases
AACCGACAAGTTCAAAAACTTAATATACTTGGTCTGCATAACATAAGATAGAGGTGATGAAAAATGCTTGATGAAAGGGA
CAGGATTATAATCGATATGC

Product: 23S rRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MRGVVERLDLEGLGIVRLDKREIHVPFTAPGDIVEIKRWRKKKRTLIATEYEVVGPSEIRTEPKCPYFGTCGGCLLQHLP
YDEQLKFKSDKLSEILGFDVEVIPSPKIYGHRNRIDVVISTNGIGFRRRGTWWDVVDIEECPVFGKASGKVLRSLREFIE
DHKPSLYEIGKNRGFLRYVVIREGKFTDELMVNLVTAEGELPEEFPDYFDYADSIYWSVNRTPSDVSYGDVERVWKKEFI
RERLDDVAYLIHPNSFFQTNSYQAVNLVREVSRLVEGEKVLDLYSGVGTFGVYLAKRGFTVEGIEINPFAVEMANRNAEL
NGIDAVFSVGEDKDVKNLAKYDTIILDPPRAGLHPKLVKKILKDEPQTIVYVSCNPKTLKLNLNELSGKYSVEEAVGLDM
FPHTPHIEVVVKLKHKVWL

Sequences:

>Translated_419_residues
MRGVVERLDLEGLGIVRLDKREIHVPFTAPGDIVEIKRWRKKKRTLIATEYEVVGPSEIRTEPKCPYFGTCGGCLLQHLP
YDEQLKFKSDKLSEILGFDVEVIPSPKIYGHRNRIDVVISTNGIGFRRRGTWWDVVDIEECPVFGKASGKVLRSLREFIE
DHKPSLYEIGKNRGFLRYVVIREGKFTDELMVNLVTAEGELPEEFPDYFDYADSIYWSVNRTPSDVSYGDVERVWKKEFI
RERLDDVAYLIHPNSFFQTNSYQAVNLVREVSRLVEGEKVLDLYSGVGTFGVYLAKRGFTVEGIEINPFAVEMANRNAEL
NGIDAVFSVGEDKDVKNLAKYDTIILDPPRAGLHPKLVKKILKDEPQTIVYVSCNPKTLKLNLNELSGKYSVEEAVGLDM
FPHTPHIEVVVKLKHKVWL
>Mature_419_residues
MRGVVERLDLEGLGIVRLDKREIHVPFTAPGDIVEIKRWRKKKRTLIATEYEVVGPSEIRTEPKCPYFGTCGGCLLQHLP
YDEQLKFKSDKLSEILGFDVEVIPSPKIYGHRNRIDVVISTNGIGFRRRGTWWDVVDIEECPVFGKASGKVLRSLREFIE
DHKPSLYEIGKNRGFLRYVVIREGKFTDELMVNLVTAEGELPEEFPDYFDYADSIYWSVNRTPSDVSYGDVERVWKKEFI
RERLDDVAYLIHPNSFFQTNSYQAVNLVREVSRLVEGEKVLDLYSGVGTFGVYLAKRGFTVEGIEINPFAVEMANRNAEL
NGIDAVFSVGEDKDVKNLAKYDTIILDPPRAGLHPKLVKKILKDEPQTIVYVSCNPKTLKLNLNELSGKYSVEEAVGLDM
FPHTPHIEVVVKLKHKVWL

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=362, Percent_Identity=28.4530386740331, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI51173878, Length=362, Percent_Identity=28.4530386740331, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI269784680, Length=400, Percent_Identity=26.75, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI269784676, Length=400, Percent_Identity=26.75, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI20149688, Length=400, Percent_Identity=26.75, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI269784678, Length=231, Percent_Identity=29.8701298701299, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1789148, Length=427, Percent_Identity=29.03981264637, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1787083, Length=369, Percent_Identity=28.9972899728997, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790403, Length=205, Percent_Identity=32.1951219512195, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24666579, Length=426, Percent_Identity=30.2816901408451, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001566
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 47913; Mature: 47913

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGVVERLDLEGLGIVRLDKREIHVPFTAPGDIVEIKRWRKKKRTLIATEYEVVGPSEIR
CCCHHHHCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCHHHC
TEPKCPYFGTCGGCLLQHLPYDEQLKFKSDKLSEILGFDVEVIPSPKIYGHRNRIDVVIS
CCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEECCCCEECCCCEEEEEEE
TNGIGFRRRGTWWDVVDIEECPVFGKASGKVLRSLREFIEDHKPSLYEIGKNRGFLRYVV
CCCCCEEECCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
IREGKFTDELMVNLVTAEGELPEEFPDYFDYADSIYWSVNRTPSDVSYGDVERVWKKEFI
EECCCCCHHHHEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
RERLDDVAYLIHPNSFFQTNSYQAVNLVREVSRLVEGEKVLDLYSGVGTFGVYLAKRGFT
HHHHHCEEEEECCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHCCCCE
VEGIEINPFAVEMANRNAELNGIDAVFSVGEDKDVKNLAKYDTIILDPPRAGLHPKLVKK
EECEEECCEEEEECCCCCCCCCCHHEEECCCCCCHHHHHHHCEEEECCCCCCCCHHHHHH
ILKDEPQTIVYVSCNPKTLKLNLNELSGKYSVEEAVGLDMFPHTPHIEVVVKLKHKVWL
HHCCCCCEEEEEEECCEEEEEEHHHCCCCEEHHHHHCCCCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MRGVVERLDLEGLGIVRLDKREIHVPFTAPGDIVEIKRWRKKKRTLIATEYEVVGPSEIR
CCCHHHHCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCHHHC
TEPKCPYFGTCGGCLLQHLPYDEQLKFKSDKLSEILGFDVEVIPSPKIYGHRNRIDVVIS
CCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEECCCCEECCCCEEEEEEE
TNGIGFRRRGTWWDVVDIEECPVFGKASGKVLRSLREFIEDHKPSLYEIGKNRGFLRYVV
CCCCCEEECCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
IREGKFTDELMVNLVTAEGELPEEFPDYFDYADSIYWSVNRTPSDVSYGDVERVWKKEFI
EECCCCCHHHHEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
RERLDDVAYLIHPNSFFQTNSYQAVNLVREVSRLVEGEKVLDLYSGVGTFGVYLAKRGFT
HHHHHCEEEEECCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHCCCCE
VEGIEINPFAVEMANRNAELNGIDAVFSVGEDKDVKNLAKYDTIILDPPRAGLHPKLVKK
EECEEECCEEEEECCCCCCCCCCHHEEECCCCCCHHHHHHHCEEEECCCCCCCCHHHHHH
ILKDEPQTIVYVSCNPKTLKLNLNELSGKYSVEEAVGLDMFPHTPHIEVVVKLKHKVWL
HHCCCCCEEEEEEECCEEEEEEHHHCCCCEEHHHHHCCCCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA