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The map label for this gene is pitB [C]

Identifier: 212224626

GI number: 212224626

Start: 1350508

End: 1351470

Strand: Reverse

Name: pitB [C]

Synonym: TON_1475

Alternate gene names: 212224626

Gene position: 1351470-1350508 (Counterclockwise)

Preceding gene: 212224636

Following gene: 212224624

Centisome position: 73.15

GC content: 53.69

Gene sequence:

>963_bases
ATGGACGGGCTAGTGATAGCAGCAATAGCAGTGGCATTCTACATAGCTTGGAACATAGGTTCGAACGACTCAGCCAACGC
TATGGGGACGGCAGTAGGTGCAGGAATACTCAGCTTCCGGCAAGCAACCCTCACCATAGCGATATTCGTCCTGATGGGTG
CCTATCTGAAGGGATACAAAGTTATGAAAACCGTCGGCAAGGGCATCGTCCCCGAAGGCTACCTCACGATGGAGATGGCG
GTAATAGCACTTCTCGCCGCTGGTGTCTGGGTCACGATAGCCACCGTCAAGGGCCTTCCGGTTTCCACGACTCAGGCCAT
AGTTGGCGGTGTTATAGGAGTTGGTCTTGCTGTCGATGCTCCAATCAACTGGTATACGCTCGTTAAAATAGCATCTGCGT
GGGTTGTCTCGCCCATACTGTCTGGGATTCTGGCGATAATCCTGTACAAGTTCTATTCCAAGGTTATTTCGAGCATAAAA
AGCGTCTCTACGATTGAGGCCCTCTACAAGGCTCTCGCTATACTCGGCGGCTCGTACATGGCCTTTAACTTCGGCACCAA
CGAGGTCGCCAATGCATCTGGACCTCTGGTCGGTGCCGGCTTCGTGGAGCCGAAGATTGCCGGAATTTTTGGCGCGATAG
CGCTCTCGATAGGCGCTCTAACTTTTAGCTACGCGGTGATGCACACTGTTGGAAAGAAGATAACCGCCCTCGGCCCGATC
TCGGCCTTCGCAGCACAGTTCGGTTCGGCAATAGCTGTGAGCCTTGCCAATATCTTCGGTCTCCCGGTGAGCTCCAGCCA
GTCCATAGTCGGTGGAGTCATAGGAGTCGGTCTCATCGCGGGAGAGGGCGTTGATAAGTCCGTCATAAAGGACATAGTCT
TCGGCTGGGTAGCGACGCCGCTGACGGCGATATTCATTGCCCTTGGAATCTTCAAGGCTTTCTCATTGGTGGGGCTGGTT
TAG

Upstream 100 bases:

>100_bases
AATTATGGCATCATTTCTCGAAAAAAGTTCGTACAGTCATTGGCAAAATTTTTTAAGCCACAGTCTAAAGGGAGATATAT
AGACTATATAGGTGATCCTT

Downstream 100 bases:

>100_bases
CCGCTTTATCTCGACCCCCAGCCTCGTCAGCTCCTTTATCATTCCCGTCTGGATGTAGGCTTCTATTTTCGTCTCCTCAC
CGTACTCAACGGACAAAACC

Product: sodium/phosphate symporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MDGLVIAAIAVAFYIAWNIGSNDSANAMGTAVGAGILSFRQATLTIAIFVLMGAYLKGYKVMKTVGKGIVPEGYLTMEMA
VIALLAAGVWVTIATVKGLPVSTTQAIVGGVIGVGLAVDAPINWYTLVKIASAWVVSPILSGILAIILYKFYSKVISSIK
SVSTIEALYKALAILGGSYMAFNFGTNEVANASGPLVGAGFVEPKIAGIFGAIALSIGALTFSYAVMHTVGKKITALGPI
SAFAAQFGSAIAVSLANIFGLPVSSSQSIVGGVIGVGLIAGEGVDKSVIKDIVFGWVATPLTAIFIALGIFKAFSLVGLV

Sequences:

>Translated_320_residues
MDGLVIAAIAVAFYIAWNIGSNDSANAMGTAVGAGILSFRQATLTIAIFVLMGAYLKGYKVMKTVGKGIVPEGYLTMEMA
VIALLAAGVWVTIATVKGLPVSTTQAIVGGVIGVGLAVDAPINWYTLVKIASAWVVSPILSGILAIILYKFYSKVISSIK
SVSTIEALYKALAILGGSYMAFNFGTNEVANASGPLVGAGFVEPKIAGIFGAIALSIGALTFSYAVMHTVGKKITALGPI
SAFAAQFGSAIAVSLANIFGLPVSSSQSIVGGVIGVGLIAGEGVDKSVIKDIVFGWVATPLTAIFIALGIFKAFSLVGLV
>Mature_320_residues
MDGLVIAAIAVAFYIAWNIGSNDSANAMGTAVGAGILSFRQATLTIAIFVLMGAYLKGYKVMKTVGKGIVPEGYLTMEMA
VIALLAAGVWVTIATVKGLPVSTTQAIVGGVIGVGLAVDAPINWYTLVKIASAWVVSPILSGILAIILYKFYSKVISSIK
SVSTIEALYKALAILGGSYMAFNFGTNEVANASGPLVGAGFVEPKIAGIFGAIALSIGALTFSYAVMHTVGKKITALGPI
SAFAAQFGSAIAVSLANIFGLPVSSSQSIVGGVIGVGLIAGEGVDKSVIKDIVFGWVATPLTAIFIALGIFKAFSLVGLV

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=154, Percent_Identity=38.961038961039, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI5803173, Length=154, Percent_Identity=38.3116883116883, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25146401, Length=189, Percent_Identity=34.9206349206349, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17539280, Length=166, Percent_Identity=37.9518072289157, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32566716, Length=162, Percent_Identity=37.037037037037, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71981576, Length=140, Percent_Identity=37.1428571428571, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319773, Length=144, Percent_Identity=30.5555555555556, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356511, Length=182, Percent_Identity=37.9120879120879, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 32577; Mature: 32577

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLVIAAIAVAFYIAWNIGSNDSANAMGTAVGAGILSFRQATLTIAIFVLMGAYLKGYK
CCCHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMKTVGKGIVPEGYLTMEMAVIALLAAGVWVTIATVKGLPVSTTQAIVGGVIGVGLAVDA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEECC
PINWYTLVKIASAWVVSPILSGILAIILYKFYSKVISSIKSVSTIEALYKALAILGGSYM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
AFNFGTNEVANASGPLVGAGFVEPKIAGIFGAIALSIGALTFSYAVMHTVGKKITALGPI
EEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SAFAAQFGSAIAVSLANIFGLPVSSSQSIVGGVIGVGLIAGEGVDKSVIKDIVFGWVATP
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LTAIFIALGIFKAFSLVGLV
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDGLVIAAIAVAFYIAWNIGSNDSANAMGTAVGAGILSFRQATLTIAIFVLMGAYLKGYK
CCCHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMKTVGKGIVPEGYLTMEMAVIALLAAGVWVTIATVKGLPVSTTQAIVGGVIGVGLAVDA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEECC
PINWYTLVKIASAWVVSPILSGILAIILYKFYSKVISSIKSVSTIEALYKALAILGGSYM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
AFNFGTNEVANASGPLVGAGFVEPKIAGIFGAIALSIGALTFSYAVMHTVGKKITALGPI
EEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SAFAAQFGSAIAVSLANIFGLPVSSSQSIVGGVIGVGLIAGEGVDKSVIKDIVFGWVATP
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LTAIFIALGIFKAFSLVGLV
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]