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The map label for this gene is wcaL [C]

Identifier: 212224471

GI number: 212224471

Start: 1198545

End: 1199687

Strand: Reverse

Name: wcaL [C]

Synonym: TON_1320

Alternate gene names: 212224471

Gene position: 1199687-1198545 (Counterclockwise)

Preceding gene: 212224472

Following gene: 212224470

Centisome position: 64.93

GC content: 53.72

Gene sequence:

>1143_bases
ATGAAGATCGCCCTCGTCAGTGACTGGTACTACCCCAAGGTCGGCGGCGTCGCCAGCCACATGCACCACCTTGCAATTCA
CCTCGGAGAGCGCGGTCATGAAGTGGCCATTGTTACCAACGATCTCGAGACCGGGAAGGAGGAGGAACTCGAAAGCCTTG
GCATCGAACTCGTTAAGGTTCCGGGGAGGGTAAGCCCTATACTTGGGATCAACATGAGCTACTCCCTCGCATCGAGTGAA
GGGCTGGGTGAATTTTTGGAGGATTACGATGTCGTGCACTCCCACCACGCCTTTACACCCCTTGCGCTCAAGGCTGTTAA
AGCCGGTAGAACCCTCGGGAAGGCGACCCTGCTGACAACCCACAGCATATCTTTCTCCTACGAATCGCGGCTCTGGGAAG
CTCTCGGACTGACTATCCCTCTCTTTAGCAGATATCTCCGCTATCCCCACGAAATAATAGCTGTCAGCAAGGCTGCAGAG
GCGTTCATCAACCACTTCACGGATGTTCCTGTGAGGGTCATCCCGAACGGGGTAGACGATGAGATCTTCAAACCCTTGAG
TAATAAGGAAAGAGACCGCCTCAAGTCAGAACTCGGTATTGAGGGAAAGGTTGTCCTGTATGTCAGCAGGATGAGCTACC
GCAAGGGACCTCAAGTTCTCATCAATGCCTTCTCGAAGATAGAGGACGCGACACTCATCTTGGTTGGCTCGGGTGAGATG
CTTCCCTTCCTGAAGGCGCAGGCTAAGTTCCTGAAGATGGAGGACAGGGTGCGCTTCCTTGGCTATGTGGAGAGCTCTCT
CCTTCCGAAGCTCTTCGGCATGGCGGATGTCTTTGTCCTTCCATCCATTACGGCGGAGGCTTTCGGTATAGTAATTCTCG
AAGCGATGGCGTCGGGCATCCCCGTTGTCGCGACGGACGTTGGGGGAATCCCCGAGATAATTAAGGAGAGTAGAAGTGGA
CTCCTCGTTCCACCAGGCAACGAGCTTTCGCTTAGGGATGCAATTCAAAAGCTCCTCAACGACGAGGAACTCGCGAAGTG
GTTCGGAAGCAACGGAAGGAAAGCTGTGGAGGAGCGTTACTCGTGGAAAAAGGTCGCTGCAGAGATAGAAAAGGCTTATG
AGGATGCACTCCTTATGGGGTAA

Upstream 100 bases:

>100_bases
ACTGGGAGTGGCCGTTTACGGGTTCAAGGACAACTACAGGCTCATGGATGCTGGAGTGGTCGCTTTGCTCATCACACACC
TGCTCGGGACGGTGGTGTGA

Downstream 100 bases:

>100_bases
GGGGATAATTATGAGGATGGAAGAACTCACCTGGCCTGATTTTGAGGAAGCTAAGAAATCAATAGATACGGTTCTTATTC
CCGTTGGGAGTGTGGAAGCC

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKIALVSDWYYPKVGGVASHMHHLAIHLGERGHEVAIVTNDLETGKEEELESLGIELVKVPGRVSPILGINMSYSLASSE
GLGEFLEDYDVVHSHHAFTPLALKAVKAGRTLGKATLLTTHSISFSYESRLWEALGLTIPLFSRYLRYPHEIIAVSKAAE
AFINHFTDVPVRVIPNGVDDEIFKPLSNKERDRLKSELGIEGKVVLYVSRMSYRKGPQVLINAFSKIEDATLILVGSGEM
LPFLKAQAKFLKMEDRVRFLGYVESSLLPKLFGMADVFVLPSITAEAFGIVILEAMASGIPVVATDVGGIPEIIKESRSG
LLVPPGNELSLRDAIQKLLNDEELAKWFGSNGRKAVEERYSWKKVAAEIEKAYEDALLMG

Sequences:

>Translated_380_residues
MKIALVSDWYYPKVGGVASHMHHLAIHLGERGHEVAIVTNDLETGKEEELESLGIELVKVPGRVSPILGINMSYSLASSE
GLGEFLEDYDVVHSHHAFTPLALKAVKAGRTLGKATLLTTHSISFSYESRLWEALGLTIPLFSRYLRYPHEIIAVSKAAE
AFINHFTDVPVRVIPNGVDDEIFKPLSNKERDRLKSELGIEGKVVLYVSRMSYRKGPQVLINAFSKIEDATLILVGSGEM
LPFLKAQAKFLKMEDRVRFLGYVESSLLPKLFGMADVFVLPSITAEAFGIVILEAMASGIPVVATDVGGIPEIIKESRSG
LLVPPGNELSLRDAIQKLLNDEELAKWFGSNGRKAVEERYSWKKVAAEIEKAYEDALLMG
>Mature_380_residues
MKIALVSDWYYPKVGGVASHMHHLAIHLGERGHEVAIVTNDLETGKEEELESLGIELVKVPGRVSPILGINMSYSLASSE
GLGEFLEDYDVVHSHHAFTPLALKAVKAGRTLGKATLLTTHSISFSYESRLWEALGLTIPLFSRYLRYPHEIIAVSKAAE
AFINHFTDVPVRVIPNGVDDEIFKPLSNKERDRLKSELGIEGKVVLYVSRMSYRKGPQVLINAFSKIEDATLILVGSGEM
LPFLKAQAKFLKMEDRVRFLGYVESSLLPKLFGMADVFVLPSITAEAFGIVILEAMASGIPVVATDVGGIPEIIKESRSG
LLVPPGNELSLRDAIQKLLNDEELAKWFGSNGRKAVEERYSWKKVAAEIEKAYEDALLMG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=386, Percent_Identity=30.8290155440415, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI299782546, Length=152, Percent_Identity=32.8947368421053, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788357, Length=132, Percent_Identity=32.5757575757576, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17532709, Length=387, Percent_Identity=27.906976744186, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI9755344, Length=397, Percent_Identity=24.9370277078086, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24654571, Length=325, Percent_Identity=29.5384615384615, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 41917; Mature: 41917

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALVSDWYYPKVGGVASHMHHLAIHLGERGHEVAIVTNDLETGKEEELESLGIELVKV
CEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCEEEEC
PGRVSPILGINMSYSLASSEGLGEFLEDYDVVHSHHAFTPLALKAVKAGRTLGKATLLTT
CCCCCCEEECCCEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCEEEEEE
HSISFSYESRLWEALGLTIPLFSRYLRYPHEIIAVSKAAEAFINHFTDVPVRVIPNGVDD
EEECCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCH
EIFKPLSNKERDRLKSELGIEGKVVLYVSRMSYRKGPQVLINAFSKIEDATLILVGSGEM
HHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
LPFLKAQAKFLKMEDRVRFLGYVESSLLPKLFGMADVFVLPSITAEAFGIVILEAMASGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PVVATDVGGIPEIIKESRSGLLVPPGNELSLRDAIQKLLNDEELAKWFGSNGRKAVEERY
CEEEECCCCCHHHHHHCCCCEEECCCCCCCHHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
SWKKVAAEIEKAYEDALLMG
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIALVSDWYYPKVGGVASHMHHLAIHLGERGHEVAIVTNDLETGKEEELESLGIELVKV
CEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCEEEEC
PGRVSPILGINMSYSLASSEGLGEFLEDYDVVHSHHAFTPLALKAVKAGRTLGKATLLTT
CCCCCCEEECCCEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCEEEEEE
HSISFSYESRLWEALGLTIPLFSRYLRYPHEIIAVSKAAEAFINHFTDVPVRVIPNGVDD
EEECCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCH
EIFKPLSNKERDRLKSELGIEGKVVLYVSRMSYRKGPQVLINAFSKIEDATLILVGSGEM
HHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
LPFLKAQAKFLKMEDRVRFLGYVESSLLPKLFGMADVFVLPSITAEAFGIVILEAMASGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PVVATDVGGIPEIIKESRSGLLVPPGNELSLRDAIQKLLNDEELAKWFGSNGRKAVEERY
CEEEECCCCCHHHHHHCCCCEEECCCCCCCHHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
SWKKVAAEIEKAYEDALLMG
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]