Click here to switch to the map view.

The map label for this gene is gph [C]

Identifier: 212224318

GI number: 212224318

Start: 1068423

End: 1069133

Strand: Reverse

Name: gph [C]

Synonym: TON_1169

Alternate gene names: 212224318

Gene position: 1069133-1068423 (Counterclockwise)

Preceding gene: 212224320

Following gene: 212224314

Centisome position: 57.87

GC content: 54.85

Gene sequence:

>711_bases
ATGCTCAGAGCGGTCTTCTTCGATTTCGTCGGCACGCTTATAACAAAGGAGGGGGAAAACGTAACCCACCAACGCATCGT
TGGGGATGTTCTCAGAAGGGCCGGCAGGGAAGATTTGGACGTCGTTAAAGTCTGGGGGGAGTACGAGGCTGAAAGCTCGG
CTCTCTTCAAGGAGCTCGCCGGAAAGCCCTACGTTAAGATAAGGGACGTCGACACCGAGGCCATGCGGAAGGTTGCCGAG
CGCTACGGCTTCTCCGTTCCCGAGGATTTCTGGGAGATAAGCATCGCCATGCACGAAAAATACGGAAAACTCTTCGACGA
TGCCGTTGAAACGATTAAGGTCCTGAAAGACCTTGGCCTCCATGTTGGCATTATAACCGACTCTGACAACGACTACATCG
AGGCTCACCTTAAGGCTCTCGGCATCTACGAGCTCTTCGACAGCATAACGACCAGCGAGGACGCTGGCTACTACAAGCCT
CATCCTAGGCCCTTCCAGCTTGCCCTGGAGAAGGCGGGAGTTAAAGCCGAGGAGGCCATTTACGTCGGCGATAATCCGGC
AAAGGACTGCGTCGGAGCGAAAAGCGTCGGCATGATTAGCGTCCTGCTCGACCCGAGTGGGACAAAGCGTGAGCTCTGGG
AGAACTGCGATTTTGTCGTCTCAAAGCTTGGAGAGGTCGTCGAGATAGCCAGGGGCATGCTCGAGAAATGA

Upstream 100 bases:

>100_bases
TGTCATCGAGGCAAGTTCGTGAAAACTTTTCGATTAAAAGTTGTCGTTGTGCCAAGGGAACGGTTTTAACGTCCCTTTCT
AAATTCCTGCGGTGGTAAAG

Downstream 100 bases:

>100_bases
GAAAGGGGTCGGCAACGGCAGGGCTCATCATCCAGTGCAAAGCGCGTTTCAGCTAGCGAAATTCTCGTCATTCCCCCTTC
TTTTCACTTCAATCTTTTGT

Product: hydrolase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLRAVFFDFVGTLITKEGENVTHQRIVGDVLRRAGREDLDVVKVWGEYEAESSALFKELAGKPYVKIRDVDTEAMRKVAE
RYGFSVPEDFWEISIAMHEKYGKLFDDAVETIKVLKDLGLHVGIITDSDNDYIEAHLKALGIYELFDSITTSEDAGYYKP
HPRPFQLALEKAGVKAEEAIYVGDNPAKDCVGAKSVGMISVLLDPSGTKRELWENCDFVVSKLGEVVEIARGMLEK

Sequences:

>Translated_236_residues
MLRAVFFDFVGTLITKEGENVTHQRIVGDVLRRAGREDLDVVKVWGEYEAESSALFKELAGKPYVKIRDVDTEAMRKVAE
RYGFSVPEDFWEISIAMHEKYGKLFDDAVETIKVLKDLGLHVGIITDSDNDYIEAHLKALGIYELFDSITTSEDAGYYKP
HPRPFQLALEKAGVKAEEAIYVGDNPAKDCVGAKSVGMISVLLDPSGTKRELWENCDFVVSKLGEVVEIARGMLEK
>Mature_236_residues
MLRAVFFDFVGTLITKEGENVTHQRIVGDVLRRAGREDLDVVKVWGEYEAESSALFKELAGKPYVKIRDVDTEAMRKVAE
RYGFSVPEDFWEISIAMHEKYGKLFDDAVETIKVLKDLGLHVGIITDSDNDYIEAHLKALGIYELFDSITTSEDAGYYKP
HPRPFQLALEKAGVKAEEAIYVGDNPAKDCVGAKSVGMISVLLDPSGTKRELWENCDFVVSKLGEVVEIARGMLEK

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=157, Percent_Identity=28.6624203821656, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR011950
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 26425; Mature: 26425

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAVFFDFVGTLITKEGENVTHQRIVGDVLRRAGREDLDVVKVWGEYEAESSALFKELA
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHC
GKPYVKIRDVDTEAMRKVAERYGFSVPEDFWEISIAMHEKYGKLFDDAVETIKVLKDLGL
CCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HVGIITDSDNDYIEAHLKALGIYELFDSITTSEDAGYYKPHPRPFQLALEKAGVKAEEAI
EEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHCEE
YVGDNPAKDCVGAKSVGMISVLLDPSGTKRELWENCDFVVSKLGEVVEIARGMLEK
EECCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRAVFFDFVGTLITKEGENVTHQRIVGDVLRRAGREDLDVVKVWGEYEAESSALFKELA
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHC
GKPYVKIRDVDTEAMRKVAERYGFSVPEDFWEISIAMHEKYGKLFDDAVETIKVLKDLGL
CCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HVGIITDSDNDYIEAHLKALGIYELFDSITTSEDAGYYKPHPRPFQLALEKAGVKAEEAI
EEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHCEE
YVGDNPAKDCVGAKSVGMISVLLDPSGTKRELWENCDFVVSKLGEVVEIARGMLEK
EECCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]