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The map label for this gene is alkA [H]

Identifier: 212224204

GI number: 212224204

Start: 973675

End: 974502

Strand: Reverse

Name: alkA [H]

Synonym: TON_1055

Alternate gene names: 212224204

Gene position: 974502-973675 (Counterclockwise)

Preceding gene: 212224205

Following gene: 212224203

Centisome position: 52.74

GC content: 49.52

Gene sequence:

>828_bases
ATGGCTGGGATTGACCTGAAGAAAACGACCCATGAGATGGTAAAGAACGGCACGTGGAAGTTCGACGGAATCTTCTGGCA
GGCCTTCGAGAACGGTCTGGCTGGCTTTGACGGAGAGAACTTCATATTCCCTGATGAATGGGGTAAGCATGAAAGGAAAG
AAGCAAAGAACAAGCTGAAATTCATCCTTGGCCTCGATACAGATCTGGACTCCTTTTACAACGAAATTGTCGATTCAAGA
TTCGCTTTTCTTATCGATGAGTTCTACGGCCTAACAGTTCCCGCCGCCCCAAGCCCGTACCAGGCCCTTGTTGAGGTGGT
AGCGCAGCAACAGATTAACTTTGAGTTCGCCCAGAGGACGATAAGGAACCTCGTCAAACTGGCCGGCAAAAGGATTGGTG
ATCTTTACGTCTTCCCGAGCGCTGAAAAAATAACGTCGCTGAGTGAAGAGGAGCTTAAGAAGGCAAAGCTTGGCTACCGC
GCGGGATACATAAAGTTCCTCACGGAGCTCTACCTTAATGGCGAGCTGAACCTTGAGCTATGGGACTGGAGGGAAGAGGA
CTCCATTAAATACCTCACAAAGTTCCGCGGAATCGGGAAGTGGAGCGCCGAGCTGTTTCTCCTCTACGGTCTAAGGAAGA
ATGTTTATCCTGCGGGTGACCTGGGGCTGAGAAGGGGGGTAGCAAAGATTTTTGGGAAGAACGTGAAAGAAGTCAAAGAG
AAAGACGTCCGCGAGCTCATTGAACCCTACGGAAAGTGGAAGAGCCTTCTGGCATTCTATGTCCTCTGCTATGATAGGAA
AACTGAGATGGAGCGGAAGAGAAAATGA

Upstream 100 bases:

>100_bases
AGGAATCCGGAGAACTCGAGATCAAACGCTCCACAGATGGTAGGCGTCTTTATGTCTTGACGCTAAAGGATATAAAGGAG
AACCCCGTTAAGCTTGACCG

Downstream 100 bases:

>100_bases
ATAGACTCCAGATAGTCTTGCCTCGTGAGAAGTTCAAATCTCTGAAGGACAAAGACCTTGAAGCCCTTATCAAAGAATAT
CTTCCAAAAGTTGAGAAAAC

Product: 3-methyladenine DNA glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MAGIDLKKTTHEMVKNGTWKFDGIFWQAFENGLAGFDGENFIFPDEWGKHERKEAKNKLKFILGLDTDLDSFYNEIVDSR
FAFLIDEFYGLTVPAAPSPYQALVEVVAQQQINFEFAQRTIRNLVKLAGKRIGDLYVFPSAEKITSLSEEELKKAKLGYR
AGYIKFLTELYLNGELNLELWDWREEDSIKYLTKFRGIGKWSAELFLLYGLRKNVYPAGDLGLRRGVAKIFGKNVKEVKE
KDVRELIEPYGKWKSLLAFYVLCYDRKTEMERKRK

Sequences:

>Translated_275_residues
MAGIDLKKTTHEMVKNGTWKFDGIFWQAFENGLAGFDGENFIFPDEWGKHERKEAKNKLKFILGLDTDLDSFYNEIVDSR
FAFLIDEFYGLTVPAAPSPYQALVEVVAQQQINFEFAQRTIRNLVKLAGKRIGDLYVFPSAEKITSLSEEELKKAKLGYR
AGYIKFLTELYLNGELNLELWDWREEDSIKYLTKFRGIGKWSAELFLLYGLRKNVYPAGDLGLRRGVAKIFGKNVKEVKE
KDVRELIEPYGKWKSLLAFYVLCYDRKTEMERKRK
>Mature_274_residues
AGIDLKKTTHEMVKNGTWKFDGIFWQAFENGLAGFDGENFIFPDEWGKHERKEAKNKLKFILGLDTDLDSFYNEIVDSRF
AFLIDEFYGLTVPAAPSPYQALVEVVAQQQINFEFAQRTIRNLVKLAGKRIGDLYVFPSAEKITSLSEEELKKAKLGYRA
GYIKFLTELYLNGELNLELWDWREEDSIKYLTKFRGIGKWSAELFLLYGLRKNVYPAGDLGLRRGVAKIFGKNVKEVKEK
DVRELIEPYGKWKSLLAFYVLCYDRKTEMERKRK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 32021; Mature: 31890

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIDLKKTTHEMVKNGTWKFDGIFWQAFENGLAGFDGENFIFPDEWGKHERKEAKNKLK
CCCCCCHHHHHHHHHCCCCEECHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHE
FILGLDTDLDSFYNEIVDSRFAFLIDEFYGLTVPAAPSPYQALVEVVAQQQINFEFAQRT
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IRNLVKLAGKRIGDLYVFPSAEKITSLSEEELKKAKLGYRAGYIKFLTELYLNGELNLEL
HHHHHHHHHHHHCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WDWREEDSIKYLTKFRGIGKWSAELFLLYGLRKNVYPAGDLGLRRGVAKIFGKNVKEVKE
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHH
KDVRELIEPYGKWKSLLAFYVLCYDRKTEMERKRK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
AGIDLKKTTHEMVKNGTWKFDGIFWQAFENGLAGFDGENFIFPDEWGKHERKEAKNKLK
CCCCCHHHHHHHHHCCCCEECHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHE
FILGLDTDLDSFYNEIVDSRFAFLIDEFYGLTVPAAPSPYQALVEVVAQQQINFEFAQRT
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IRNLVKLAGKRIGDLYVFPSAEKITSLSEEELKKAKLGYRAGYIKFLTELYLNGELNLEL
HHHHHHHHHHHHCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WDWREEDSIKYLTKFRGIGKWSAELFLLYGLRKNVYPAGDLGLRRGVAKIFGKNVKEVKE
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHH
KDVRELIEPYGKWKSLLAFYVLCYDRKTEMERKRK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377 [H]