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The map label for this gene is alaXM [H]

Identifier: 212224048

GI number: 212224048

Start: 828943

End: 829587

Strand: Reverse

Name: alaXM [H]

Synonym: TON_0899

Alternate gene names: 212224048

Gene position: 829587-828943 (Counterclockwise)

Preceding gene: 212224049

Following gene: 212224026

Centisome position: 44.9

GC content: 53.8

Gene sequence:

>645_bases
ATGACGAGGAAGCTCTACTATGAGGACGCCTACCTTAAGGAAGCAAAAGCGAAGGTTCTTGAGATTAGGGAGGGTGCACT
CCTCCTTGACCAGACGATTTTCTACCCTACCGGCGGCGGTCAGCCCCACGACAGGGGGACGATAAACGGCGTCGAAGTTC
TGGACGTCTATAAGGACGATGCTGGAAACGTCTGGCACGCCGTTGCTGAGCCGGGGAAGTTCAAGCCAGGTGACGAGGTC
GAGCTCAAAATAGACTGGGACTACAGGTACAAGCTCATGCGCATCCACTCGGCCATGCACCTCCTCGAGCACGTCCTCAA
TGAGGTTCTTGGGGAAGGCAACTGGGAGCTCTATGGCAGTGGGATGAGCGCTGAGAAAGGTAGGTACGACATCCTCTATC
CGGAAAACGTTAACCAGTACAAGGAGAAGATCATAGAGCTGTTCAACAAATACGTCGACGAAGGCGGCGAGATGAAGATA
TGGTGGGAAGGAGAGACGAGATACACTCAGATAAGGGACTTCGAGGTCATCCCTTGTGGCGGAACGCACGTGAAGGACAT
AAAGGAGATAGGACACCTCAAGAAGCTCAAGCGCTCCAGCCTCGGAAAGGGCAAGCAGAGGCTTGAGATCTGGCTTGAGG
ACTGA

Upstream 100 bases:

>100_bases
CTTATAGGTCTGGCCATCTTCATCTGGAAGAAGCACTGAGCATTTCTTTCTTTTCGATATTCATTTAAACCTGCTTTCCA
ACTTTTCTAAGGTGGTCTTA

Downstream 100 bases:

>100_bases
GGTCTCTTTGAGTTTTAGATTATGCTCTAGGGCCGTTCTTTTCCATTCCCTGTTACAGGGACTTTTCTCGGAGAGCAGCT
TTATGGCCCCGCTCGTAAGG

Product: alanyl-tRNA synthetase-related protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTRKLYYEDAYLKEAKAKVLEIREGALLLDQTIFYPTGGGQPHDRGTINGVEVLDVYKDDAGNVWHAVAEPGKFKPGDEV
ELKIDWDYRYKLMRIHSAMHLLEHVLNEVLGEGNWELYGSGMSAEKGRYDILYPENVNQYKEKIIELFNKYVDEGGEMKI
WWEGETRYTQIRDFEVIPCGGTHVKDIKEIGHLKKLKRSSLGKGKQRLEIWLED

Sequences:

>Translated_214_residues
MTRKLYYEDAYLKEAKAKVLEIREGALLLDQTIFYPTGGGQPHDRGTINGVEVLDVYKDDAGNVWHAVAEPGKFKPGDEV
ELKIDWDYRYKLMRIHSAMHLLEHVLNEVLGEGNWELYGSGMSAEKGRYDILYPENVNQYKEKIIELFNKYVDEGGEMKI
WWEGETRYTQIRDFEVIPCGGTHVKDIKEIGHLKKLKRSSLGKGKQRLEIWLED
>Mature_213_residues
TRKLYYEDAYLKEAKAKVLEIREGALLLDQTIFYPTGGGQPHDRGTINGVEVLDVYKDDAGNVWHAVAEPGKFKPGDEVE
LKIDWDYRYKLMRIHSAMHLLEHVLNEVLGEGNWELYGSGMSAEKGRYDILYPENVNQYKEKIIELFNKYVDEGGEMKIW
WEGETRYTQIRDFEVIPCGGTHVKDIKEIGHLKKLKRSSLGKGKQRLEIWLED

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged Gly-tRNA(Ala) and Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=188, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI217416402, Length=188, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789048, Length=213, Percent_Identity=27.2300469483568, Blast_Score=65, Evalue=3e-12,
Organism=Drosophila melanogaster, GI18543305, Length=231, Percent_Identity=26.4069264069264, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582809, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45552267, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 24838; Mature: 24706

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKLYYEDAYLKEAKAKVLEIREGALLLDQTIFYPTGGGQPHDRGTINGVEVLDVYKDD
CCCEEECCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEECC
AGNVWHAVAEPGKFKPGDEVELKIDWDYRYKLMRIHSAMHLLEHVLNEVLGEGNWELYGS
CCCEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GMSAEKGRYDILYPENVNQYKEKIIELFNKYVDEGGEMKIWWEGETRYTQIRDFEVIPCG
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECEEEEECC
GTHVKDIKEIGHLKKLKRSSLGKGKQRLEIWLED
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure 
TRKLYYEDAYLKEAKAKVLEIREGALLLDQTIFYPTGGGQPHDRGTINGVEVLDVYKDD
CCEEECCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEECC
AGNVWHAVAEPGKFKPGDEVELKIDWDYRYKLMRIHSAMHLLEHVLNEVLGEGNWELYGS
CCCEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GMSAEKGRYDILYPENVNQYKEKIIELFNKYVDEGGEMKIWWEGETRYTQIRDFEVIPCG
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECEEEEECC
GTHVKDIKEIGHLKKLKRSSLGKGKQRLEIWLED
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]