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The map label for this gene is thiF [H]

Identifier: 212224003

GI number: 212224003

Start: 790922

End: 791896

Strand: Reverse

Name: thiF [H]

Synonym: TON_0854

Alternate gene names: 212224003

Gene position: 791896-790922 (Counterclockwise)

Preceding gene: 212224021

Following gene: 212224002

Centisome position: 42.86

GC content: 50.97

Gene sequence:

>975_bases
ATGGTGGATGTAAAGATGGACTTTTCGAGGCACTTCCCGATAATCGGTATGGATGGCCAAAGAAAGCTCGGCGAGAGCAC
GGTTGCAGTTGTAGGTGCCGGAGCACTGGGGAGCTGGGAGGTCTACTTCCTCCACAAACTGGGCGTTGGAAGAATAATAG
TGGTAGACAGAGACTTCGTAGACGAGAGCGACCTTCCGAGGACTATATATACGAGTGATGATGTAGGAAAGCCCAAAGTG
GAAGTCCTGAAGGAGCGCTTTGAGAACGTTATTGGCTACTTTGAGGACTTAAACCCGTCAACGGTGCACCTTCTGGACGA
AGCTGACCTCATTATAGACGGAACGGACAACATTTACACGAGACAGGTAATAAACGACTATGCGGTGAAGAGCAACAAAC
CGTGGATTTACGTCGGTATCTTGGCGACTTACGGTAACTTGATGCCCATAATTCCAGGAAAGACTGCCTGTTTCCGCTGT
TTGATGCCGAAGCTGCCCTCAAGACCCATGCCAACGTGCGCCGTCGCAGGGATTATGAGCTACGTTCCTCCCCTGGCTGC
TTCGCTGGCCGTCACTCTAGCGGCTAAGATTCTCTTAGGTGGGGAAGTGAAGAGTGAGCTGATTTTCTTCGACACCAAGA
CACTCGACTTTGAGAAGGTGGAGATCCCGAGGAGGGAGGACTGTGAGGTCTGTGTCAGGCACAACTTCACGTTCCTGGAG
AAGCAGATGAAGATAGAGCACATGTGTGACGGTTCGATTCAAATAACTCCCCCCGAACGGATGAGCATAGACCTTGATGA
GCTCGCCAAGAGGCTCGATGCCCTCGGCATTGAGTATATCAAGACGTCGCAGTTCATCCAGTTCGAGGACGACTACGCTG
AGATCCTGATATTTACGAGCGGCAGGATGATAATCCGCGGTGCTGAGGACGAAAAGGAAGCCAAAAACTTCTTTGCACGC
TATTTGGGTGGTTGA

Upstream 100 bases:

>100_bases
TAATATCCTTTTCCGAAGTTGGTATTTCCCAGAACTGTCCAGAACTTTTGGTTTTCAAAAGCCTTTTTTGAGCCTCGTAG
AAGCCTCCAATGGTGGGAGC

Downstream 100 bases:

>100_bases
GATGATACTGGTACGCTATGGCGAGATAGCAGTAAAGGGGGGCAAAAGAAGAGAGTTTGAAAGGAGGCTCGTAGAGAACA
TCCTCGCTGCCTTAGAGAGG

Product: moeb

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MVDVKMDFSRHFPIIGMDGQRKLGESTVAVVGAGALGSWEVYFLHKLGVGRIIVVDRDFVDESDLPRTIYTSDDVGKPKV
EVLKERFENVIGYFEDLNPSTVHLLDEADLIIDGTDNIYTRQVINDYAVKSNKPWIYVGILATYGNLMPIIPGKTACFRC
LMPKLPSRPMPTCAVAGIMSYVPPLAASLAVTLAAKILLGGEVKSELIFFDTKTLDFEKVEIPRREDCEVCVRHNFTFLE
KQMKIEHMCDGSIQITPPERMSIDLDELAKRLDALGIEYIKTSQFIQFEDDYAEILIFTSGRMIIRGAEDEKEAKNFFAR
YLGG

Sequences:

>Translated_324_residues
MVDVKMDFSRHFPIIGMDGQRKLGESTVAVVGAGALGSWEVYFLHKLGVGRIIVVDRDFVDESDLPRTIYTSDDVGKPKV
EVLKERFENVIGYFEDLNPSTVHLLDEADLIIDGTDNIYTRQVINDYAVKSNKPWIYVGILATYGNLMPIIPGKTACFRC
LMPKLPSRPMPTCAVAGIMSYVPPLAASLAVTLAAKILLGGEVKSELIFFDTKTLDFEKVEIPRREDCEVCVRHNFTFLE
KQMKIEHMCDGSIQITPPERMSIDLDELAKRLDALGIEYIKTSQFIQFEDDYAEILIFTSGRMIIRGAEDEKEAKNFFAR
YLGG
>Mature_324_residues
MVDVKMDFSRHFPIIGMDGQRKLGESTVAVVGAGALGSWEVYFLHKLGVGRIIVVDRDFVDESDLPRTIYTSDDVGKPKV
EVLKERFENVIGYFEDLNPSTVHLLDEADLIIDGTDNIYTRQVINDYAVKSNKPWIYVGILATYGNLMPIIPGKTACFRC
LMPKLPSRPMPTCAVAGIMSYVPPLAASLAVTLAAKILLGGEVKSELIFFDTKTLDFEKVEIPRREDCEVCVRHNFTFLE
KQMKIEHMCDGSIQITPPERMSIDLDELAKRLDALGIEYIKTSQFIQFEDDYAEILIFTSGRMIIRGAEDEKEAKNFFAR
YLGG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=284, Percent_Identity=27.8169014084507, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI87082356, Length=243, Percent_Identity=32.9218106995885, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787048, Length=240, Percent_Identity=28.75, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540406, Length=241, Percent_Identity=29.045643153527, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320598, Length=176, Percent_Identity=31.25, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321903, Length=273, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322639, Length=191, Percent_Identity=26.7015706806283, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325323, Length=168, Percent_Identity=32.7380952380952, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582879, Length=245, Percent_Identity=28.9795918367347, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 36452; Mature: 36452

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDVKMDFSRHFPIIGMDGQRKLGESTVAVVGAGALGSWEVYFLHKLGVGRIIVVDRDFV
CEEEEECHHCCCCEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCC
DESDLPRTIYTSDDVGKPKVEVLKERFENVIGYFEDLNPSTVHLLDEADLIIDGTDNIYT
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCHH
RQVINDYAVKSNKPWIYVGILATYGNLMPIIPGKTACFRCLMPKLPSRPMPTCAVAGIMS
HHHHHHHHHCCCCCEEEEEEEHHCCCEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
YVPPLAASLAVTLAAKILLGGEVKSELIFFDTKTLDFEKVEIPRREDCEVCVRHNFTFLE
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCHHHHHCCCHHHH
KQMKIEHMCDGSIQITPPERMSIDLDELAKRLDALGIEYIKTSQFIQFEDDYAEILIFTS
HHHHHHHHCCCCEEECCCCCCEECHHHHHHHHHHHCHHEEECCCEEEECCCCEEEEEEEC
GRMIIRGAEDEKEAKNFFARYLGG
CEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MVDVKMDFSRHFPIIGMDGQRKLGESTVAVVGAGALGSWEVYFLHKLGVGRIIVVDRDFV
CEEEEECHHCCCCEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCC
DESDLPRTIYTSDDVGKPKVEVLKERFENVIGYFEDLNPSTVHLLDEADLIIDGTDNIYT
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCHH
RQVINDYAVKSNKPWIYVGILATYGNLMPIIPGKTACFRCLMPKLPSRPMPTCAVAGIMS
HHHHHHHHHCCCCCEEEEEEEHHCCCEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
YVPPLAASLAVTLAAKILLGGEVKSELIFFDTKTLDFEKVEIPRREDCEVCVRHNFTFLE
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCHHHHHCCCHHHH
KQMKIEHMCDGSIQITPPERMSIDLDELAKRLDALGIEYIKTSQFIQFEDDYAEILIFTS
HHHHHHHHCCCCEEECCCCCCEECHHHHHHHHHHHCHHEEECCCEEEECCCCEEEEEEEC
GRMIIRGAEDEKEAKNFFARYLGG
CEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]