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The map label for this gene is thiI [H]

Identifier: 212224002

GI number: 212224002

Start: 789829

End: 790920

Strand: Reverse

Name: thiI [H]

Synonym: TON_0853

Alternate gene names: 212224002

Gene position: 790920-789829 (Counterclockwise)

Preceding gene: 212224003

Following gene: 212224001

Centisome position: 42.81

GC content: 48.35

Gene sequence:

>1092_bases
ATGATACTGGTACGCTATGGCGAGATAGCAGTAAAGGGGGGCAAAAGAAGAGAGTTTGAAAGGAGGCTCGTAGAGAACAT
CCTCGCTGCCTTAGAGAGGAAAGGGATTAAAGCAAAGGCGAGGTTGATTAGAGGACGAATCCTCATTGAGGCCCCCGATG
AAGCGGCTGAGATTATTGCAAAAGTCCCGGGCGTTGTTTCGGTTTCGCCTGCGAGGGAGATGAACTACGAGGAAGTGCCG
GACTATCTGAAAGAAGCCCTGAAAGGCCTGAATCCGAGAAGCTTTAAGGTGGAAACCCAGCGGCTGGATAAGGCGTTCCT
CAAGACTTCTGTTGAGGTTAACAGGGAAATAGGGGCGTTCATTGTGAGAGAATTCGGCTGGAAAGTAGATCTGGAAAACC
CCGAGCTCACGATAGGAATTGAAATTATAAAGGGAAAGGCCTACGTCTTCTTTGAGAAGATTAAAGGAGTCGGTGGGCTT
CCAGTTGGCACGCAGGGGAAGGTTGTGGTTCTGCTCAGCGGAGGCATAGATTCACCCGTTGCCGCATTCCTCATGATGAA
GAGGGGTGCAGAAATAATAGCGGTCCATTTTGACCAGGGAAAGAACGCAAAGAATGTTGTTGAGAAAACCGTTGAGATAC
TCAATGATTACTCCCCAAAAGACATTGAGCTCATTGTGGAGAACCACTTTGAAGTTCTGAGACCCTACGTGGCCGTCCTC
AGCAGGATAAACATGCTGGAATGGACATGCGTCCTGTGCAAGGTCGCTATGCTGAGGAGAGCCGCGGAGATAGCAAGGGA
AGAAGGTGCGCTGGGCATTGTTACTGGGGATTCCCTTGGCCAAGTGGCTTCTCAGACACTGGCTAACCTTTACTTTGAGA
CCATGAGCGTCCGCTTTCCAGTACACAGGCCGCTTTTAGGTATGGACAAGGAAGAGATAGTTAAGATAGCGAGGGAAATT
GGCACATATCAAGCCTTTTTAGAGTACCCTTACTGTGATTGTCCCTTCAGACCAGAGAGAGTTGTAACTCAGGGGAAGTA
TGGGGAATTCTTGAAGATCTTGGATATTTTGGAAAAGGAAGGAATAATTTAA

Upstream 100 bases:

>100_bases
GCTGAGATCCTGATATTTACGAGCGGCAGGATGATAATCCGCGGTGCTGAGGACGAAAAGGAAGCCAAAAACTTCTTTGC
ACGCTATTTGGGTGGTTGAG

Downstream 100 bases:

>100_bases
AGTTTTTCAAAAATCTGAGGAGAAATCTTTTAAAGTAACCGGGTGTAACTATAGGAGGGTGATGGCCATGCTGAGTGAAA
GAATGTTGAAAGCTCTAAAT

Product: thiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MILVRYGEIAVKGGKRREFERRLVENILAALERKGIKAKARLIRGRILIEAPDEAAEIIAKVPGVVSVSPAREMNYEEVP
DYLKEALKGLNPRSFKVETQRLDKAFLKTSVEVNREIGAFIVREFGWKVDLENPELTIGIEIIKGKAYVFFEKIKGVGGL
PVGTQGKVVVLLSGGIDSPVAAFLMMKRGAEIIAVHFDQGKNAKNVVEKTVEILNDYSPKDIELIVENHFEVLRPYVAVL
SRINMLEWTCVLCKVAMLRRAAEIAREEGALGIVTGDSLGQVASQTLANLYFETMSVRFPVHRPLLGMDKEEIVKIAREI
GTYQAFLEYPYCDCPFRPERVVTQGKYGEFLKILDILEKEGII

Sequences:

>Translated_363_residues
MILVRYGEIAVKGGKRREFERRLVENILAALERKGIKAKARLIRGRILIEAPDEAAEIIAKVPGVVSVSPAREMNYEEVP
DYLKEALKGLNPRSFKVETQRLDKAFLKTSVEVNREIGAFIVREFGWKVDLENPELTIGIEIIKGKAYVFFEKIKGVGGL
PVGTQGKVVVLLSGGIDSPVAAFLMMKRGAEIIAVHFDQGKNAKNVVEKTVEILNDYSPKDIELIVENHFEVLRPYVAVL
SRINMLEWTCVLCKVAMLRRAAEIAREEGALGIVTGDSLGQVASQTLANLYFETMSVRFPVHRPLLGMDKEEIVKIAREI
GTYQAFLEYPYCDCPFRPERVVTQGKYGEFLKILDILEKEGII
>Mature_363_residues
MILVRYGEIAVKGGKRREFERRLVENILAALERKGIKAKARLIRGRILIEAPDEAAEIIAKVPGVVSVSPAREMNYEEVP
DYLKEALKGLNPRSFKVETQRLDKAFLKTSVEVNREIGAFIVREFGWKVDLENPELTIGIEIIKGKAYVFFEKIKGVGGL
PVGTQGKVVVLLSGGIDSPVAAFLMMKRGAEIIAVHFDQGKNAKNVVEKTVEILNDYSPKDIELIVENHFEVLRPYVAVL
SRINMLEWTCVLCKVAMLRRAAEIAREEGALGIVTGDSLGQVASQTLANLYFETMSVRFPVHRPLLGMDKEEIVKIAREI
GTYQAFLEYPYCDCPFRPERVVTQGKYGEFLKILDILEKEGII

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=300, Percent_Identity=29.6666666666667, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 40708; Mature: 40708

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVRYGEIAVKGGKRREFERRLVENILAALERKGIKAKARLIRGRILIEAPDEAAEIIA
CEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEECCCHHHHHHH
KVPGVVSVSPAREMNYEEVPDYLKEALKGLNPRSFKVETQRLDKAFLKTSVEVNREIGAF
HCCCCEEECCHHHCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
IVREFGWKVDLENPELTIGIEIIKGKAYVFFEKIKGVGGLPVGTQGKVVVLLSGGIDSPV
HHHHCCCEEECCCCCEEEEEEEEECCEEEEEEHHCCCCCCCCCCCCCEEEEEECCCCHHH
AAFLMMKRGAEIIAVHFDQGKNAKNVVEKTVEILNDYSPKDIELIVENHFEVLRPYVAVL
HHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SRINMLEWTCVLCKVAMLRRAAEIAREEGALGIVTGDSLGQVASQTLANLYFETMSVRFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHEECC
VHRPLLGMDKEEIVKIAREIGTYQAFLEYPYCDCPFRPERVVTQGKYGEFLKILDILEKE
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHC
GII
CCC
>Mature Secondary Structure
MILVRYGEIAVKGGKRREFERRLVENILAALERKGIKAKARLIRGRILIEAPDEAAEIIA
CEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEECCCHHHHHHH
KVPGVVSVSPAREMNYEEVPDYLKEALKGLNPRSFKVETQRLDKAFLKTSVEVNREIGAF
HCCCCEEECCHHHCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
IVREFGWKVDLENPELTIGIEIIKGKAYVFFEKIKGVGGLPVGTQGKVVVLLSGGIDSPV
HHHHCCCEEECCCCCEEEEEEEEECCEEEEEEHHCCCCCCCCCCCCCEEEEEECCCCHHH
AAFLMMKRGAEIIAVHFDQGKNAKNVVEKTVEILNDYSPKDIELIVENHFEVLRPYVAVL
HHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SRINMLEWTCVLCKVAMLRRAAEIAREEGALGIVTGDSLGQVASQTLANLYFETMSVRFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHEECC
VHRPLLGMDKEEIVKIAREIGTYQAFLEYPYCDCPFRPERVVTQGKYGEFLKILDILEKE
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHC
GII
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]