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The map label for this gene is rio1 [H]

Identifier: 212223915

GI number: 212223915

Start: 713337

End: 714116

Strand: Reverse

Name: rio1 [H]

Synonym: TON_0766

Alternate gene names: 212223915

Gene position: 714116-713337 (Counterclockwise)

Preceding gene: 212223916

Following gene: 212223914

Centisome position: 38.65

GC content: 53.59

Gene sequence:

>780_bases
ATGCGCGAGGAGTTCATCGAGCGGGAAGTCGAGGAGATGCTCGGTCTCACGGAGAGACGCGAAAAGGACAGCGAGCTCTA
CAAGATAGCCAACGAGGTCTTCGACAGGACGACCAAGGAAACCCTCGCCTATCTCCACCGCAGAGGTAAGATAGAGAGCC
TCTACGGTGTGATAAGCACTGGCAAGGAGGCTAACGTCTTCGCTGGAATAGACAGCGAGGGCAACAGGGTGGCGGTGAAG
ATATACCGGACCTACACCACCGAGTTCCGCAGAATATGGGAGTACCTAGCTGCCGACCCGAGAGTTGGCTACTTGCCCAA
GGATATGAGAAAGCTCGTCTTTGTCTGGACGAGGAGGGAATATAAAAACCTCCGGAGGGCGATTAAATACGCGGTCCGCG
TTCCCGAGCCGATAATCTTCCGCAACAACGTCCTCGTCATGGAGTTCATAGGCGAGGAGCTTCCTGCTCCCCGCTTGAAG
GACGTCGAAAGGGAGCTGGCTAAAGAGGACTTCGAGGAGCTCTACGACTTTACGATGGGTGTAATCGAGCGCCTCTGGAA
GAGGGGAGACATGGTGCATGGGGACCTGAGCGAGTACAATATCCTGCTCCATGATGGCCCTGTGGTTATAGACTGGTCCC
AGGCGACCGTGAAGAGGAATAGGATGAGCGTTGAGCTCCTGAAGAGAGACTTGAGGAACATCATCAATTACTTTGGTAAA
AAGGGCGTTGATGTTGATGATTTCCACGAGAAGTTTAAGGAACTCGTTGAGAGCGGGTGA

Upstream 100 bases:

>100_bases
ACAGGTTGACTGGCTCCTGAGGAAGGGCAAGATAAGCCAGGATTTCCTCACGGGCGGCGAGCTGCTCTTCTGAGCTCTCA
AAACCCTTTGAGTGATAGCG

Downstream 100 bases:

>100_bases
GAGGTATGGACGAGTTTGAGAGACTGCTCAAGAAGTACGAGCGCGTTGATAAGGACGGAAGACCCCTCAAGAACAATGAG
GAAGAGGAGATAACCTATGC

Product: serine/threonine protein kinase

Products: NA

Alternate protein names: AfRio1 [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MREEFIEREVEEMLGLTERREKDSELYKIANEVFDRTTKETLAYLHRRGKIESLYGVISTGKEANVFAGIDSEGNRVAVK
IYRTYTTEFRRIWEYLAADPRVGYLPKDMRKLVFVWTRREYKNLRRAIKYAVRVPEPIIFRNNVLVMEFIGEELPAPRLK
DVERELAKEDFEELYDFTMGVIERLWKRGDMVHGDLSEYNILLHDGPVVIDWSQATVKRNRMSVELLKRDLRNIINYFGK
KGVDVDDFHEKFKELVESG

Sequences:

>Translated_259_residues
MREEFIEREVEEMLGLTERREKDSELYKIANEVFDRTTKETLAYLHRRGKIESLYGVISTGKEANVFAGIDSEGNRVAVK
IYRTYTTEFRRIWEYLAADPRVGYLPKDMRKLVFVWTRREYKNLRRAIKYAVRVPEPIIFRNNVLVMEFIGEELPAPRLK
DVERELAKEDFEELYDFTMGVIERLWKRGDMVHGDLSEYNILLHDGPVVIDWSQATVKRNRMSVELLKRDLRNIINYFGK
KGVDVDDFHEKFKELVESG
>Mature_259_residues
MREEFIEREVEEMLGLTERREKDSELYKIANEVFDRTTKETLAYLHRRGKIESLYGVISTGKEANVFAGIDSEGNRVAVK
IYRTYTTEFRRIWEYLAADPRVGYLPKDMRKLVFVWTRREYKNLRRAIKYAVRVPEPIIFRNNVLVMEFIGEELPAPRLK
DVERELAKEDFEELYDFTMGVIERLWKRGDMVHGDLSEYNILLHDGPVVIDWSQATVKRNRMSVELLKRDLRNIINYFGK
KGVDVDDFHEKFKELVESG

Specific function: Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis [H]

COG id: COG1718

COG function: function code TD; Serine/threonine protein kinase involved in cell cycle control

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI23510356, Length=250, Percent_Identity=35.6, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI22325377, Length=232, Percent_Identity=32.7586206896552, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI23510358, Length=143, Percent_Identity=37.0629370629371, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI229331971, Length=227, Percent_Identity=25.9911894273128, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI229331967, Length=227, Percent_Identity=25.9911894273128, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532073, Length=244, Percent_Identity=35.2459016393443, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17556977, Length=228, Percent_Identity=37.280701754386, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71989043, Length=160, Percent_Identity=35, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324693, Length=240, Percent_Identity=33.75, Blast_Score=115, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24662811, Length=219, Percent_Identity=40.1826484018265, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21356879, Length=219, Percent_Identity=40.1826484018265, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19920700, Length=218, Percent_Identity=29.8165137614679, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24649986, Length=247, Percent_Identity=25.5060728744939, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR018934
- InterPro:   IPR000687
- InterPro:   IPR018935 [H]

Pfam domain/function: PF01163 RIO1 [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 30740; Mature: 30740

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00109 PROTEIN_KINASE_TYR ; PS50011 PROTEIN_KINASE_DOM ; PS01245 RIO1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREEFIEREVEEMLGLTERREKDSELYKIANEVFDRTTKETLAYLHRRGKIESLYGVIST
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
GKEANVFAGIDSEGNRVAVKIYRTYTTEFRRIWEYLAADPRVGYLPKDMRKLVFVWTRRE
CCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
YKNLRRAIKYAVRVPEPIIFRNNVLVMEFIGEELPAPRLKDVERELAKEDFEELYDFTMG
HHHHHHHHHHHHCCCCCEEEECCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VIERLWKRGDMVHGDLSEYNILLHDGPVVIDWSQATVKRNRMSVELLKRDLRNIINYFGK
HHHHHHHCCCCCCCCHHHCEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCC
KGVDVDDFHEKFKELVESG
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MREEFIEREVEEMLGLTERREKDSELYKIANEVFDRTTKETLAYLHRRGKIESLYGVIST
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
GKEANVFAGIDSEGNRVAVKIYRTYTTEFRRIWEYLAADPRVGYLPKDMRKLVFVWTRRE
CCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
YKNLRRAIKYAVRVPEPIIFRNNVLVMEFIGEELPAPRLKDVERELAKEDFEELYDFTMG
HHHHHHHHHHHHCCCCCEEEECCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VIERLWKRGDMVHGDLSEYNILLHDGPVVIDWSQATVKRNRMSVELLKRDLRNIINYFGK
HHHHHHHCCCCCCCCHHHCEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCC
KGVDVDDFHEKFKELVESG
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]