| Definition | Thermococcus onnurineus NA1, complete genome. |
|---|---|
| Accession | NC_011529 |
| Length | 1,847,607 |
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The map label for this gene is tuf [H]
Identifier: 212223901
GI number: 212223901
Start: 700151
End: 701437
Strand: Reverse
Name: tuf [H]
Synonym: TON_0752
Alternate gene names: 212223901
Gene position: 701437-700151 (Counterclockwise)
Preceding gene: 212223902
Following gene: 212223900
Centisome position: 37.96
GC content: 59.05
Gene sequence:
>1287_bases ATGGCAAAGGAGAAGCCACACGTTAACATAGTCTTTATCGGCCACGTCGACCACGGAAAGAGCACCACCATCGGAAGGCT GCTCTTCGACACCGCCAACATACCGGAGAACATCATCAAGAAGTTCGAGGAGATGGGTGAGAAGGGTAAGTCCTTCAAGT TCGCTTGGGTCATGGACAGGCTCAAGGAGGAGAGGGAGAGAGGTATCACCATCGACGTCGCCCACACCAAGTTCGAGACC CCGCACAGGTACATCACCATCATCGACGCTCCGGGCCACAGGGACTTCGTTAAGAACATGATCACCGGTGCCAGCCAGGC CGACGCTGCCGTTCTCGTGGTTGCTGCCACCGACGGTGTCATGCCGCAGACCAAGGAGCACGCCTTCCTTGCCAGGACCC TTGGTATCGGCCACATCATCGTCGCTATCAACAAGATGGACATGGTTGACTACGACGAGAAGAAGTTCAAGCAGGTCTCC GAGCAGGTTAAGAAGCTCCTCATGATGCTCGGTTACAAGGACTTCCCGATCATCCCGATCAGCGCTTGGGAGGGCGACAA CGTCGTTAAGAAGAGCGACAAGATGCCCTGGTACAATGGCCCGACCCTCATCGAGGCCCTCGACCAGATCCCAGAGCCGC CGAAGCCGACCGACAAGCCGCTCCGCATCCCGATCCAGGACGTCTACTCCATTAAGGGTGTCGGTACCGTTCCGGTCGGC CGTGTCGAGACCGGTGTCCTCCGCGTCGGTGACGTCGTCATCTTCGAGCCGGCCAGCACCATCTTCCACAAGCCGATCCA GGGTGAGGTTAAGAGCATCGAGATGCACCACGAGCCAATGCAGGAGGCCCTTCCGGGTGACAACATCGGATTCAACGTCA GGGGTGTCGGTAAGAACGACATAAAGCGCGGTGACGTTGCTGGTCACACCAACAACCCGCCGACCGTTGTCAGGCCGAAG GACACCTTCAAGGCCCAGATCATCGTCCTCAACCACCCGACCGCTATCACCATCGGTTACACCCCGGTCCTCCACGCCCA CACCCTCCAGGTCGCCGTCAGGTTCGAGCAGCTCCTTGCCAAGCTCGACCCGAGGACCGGTAACGTCGTCGAGGAGAACC CGCAGTTCATCAAGACCGGTGACTCTGCCATCGTCGTCCTCAGGCCGACCAAGCCGATGGTCATCGAGCCGGTCAAGGAG ATCCCGCAGATGGGCAGGTTCGCCATCCGTGACATGGGCCAGACCGTCGCTGCCGGTATGGTTATCTCCATCCAGAAGGC CGAGTGA
Upstream 100 bases:
>100_bases GAAAAAGGCGGGTTTCCCGCCCGAGGGAGCCGAAGTTCGAAAGATTTAAAAAGCCATCCTAGTCAAACGAGACTAGACCA AAATTTGGAGGTGTTTGGAA
Downstream 100 bases:
>100_bases AGGCCTTCTCCTTCCTTTACTTCCTTAGCTCTTAGGAGGGACGGAAATGCAGAAGGCAAGGATTAAGCTCGCAAGCACTA ACATTAAGGCCCTCAACGAG
Product: elongation factor 1-alpha
Products: NA
Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]
Number of amino acids: Translated: 428; Mature: 427
Protein sequence:
>428_residues MAKEKPHVNIVFIGHVDHGKSTTIGRLLFDTANIPENIIKKFEEMGEKGKSFKFAWVMDRLKEERERGITIDVAHTKFET PHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGVMPQTKEHAFLARTLGIGHIIVAINKMDMVDYDEKKFKQVS EQVKKLLMMLGYKDFPIIPISAWEGDNVVKKSDKMPWYNGPTLIEALDQIPEPPKPTDKPLRIPIQDVYSIKGVGTVPVG RVETGVLRVGDVVIFEPASTIFHKPIQGEVKSIEMHHEPMQEALPGDNIGFNVRGVGKNDIKRGDVAGHTNNPPTVVRPK DTFKAQIIVLNHPTAITIGYTPVLHAHTLQVAVRFEQLLAKLDPRTGNVVEENPQFIKTGDSAIVVLRPTKPMVIEPVKE IPQMGRFAIRDMGQTVAAGMVISIQKAE
Sequences:
>Translated_428_residues MAKEKPHVNIVFIGHVDHGKSTTIGRLLFDTANIPENIIKKFEEMGEKGKSFKFAWVMDRLKEERERGITIDVAHTKFET PHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGVMPQTKEHAFLARTLGIGHIIVAINKMDMVDYDEKKFKQVS EQVKKLLMMLGYKDFPIIPISAWEGDNVVKKSDKMPWYNGPTLIEALDQIPEPPKPTDKPLRIPIQDVYSIKGVGTVPVG RVETGVLRVGDVVIFEPASTIFHKPIQGEVKSIEMHHEPMQEALPGDNIGFNVRGVGKNDIKRGDVAGHTNNPPTVVRPK DTFKAQIIVLNHPTAITIGYTPVLHAHTLQVAVRFEQLLAKLDPRTGNVVEENPQFIKTGDSAIVVLRPTKPMVIEPVKE IPQMGRFAIRDMGQTVAAGMVISIQKAE >Mature_427_residues AKEKPHVNIVFIGHVDHGKSTTIGRLLFDTANIPENIIKKFEEMGEKGKSFKFAWVMDRLKEERERGITIDVAHTKFETP HRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGVMPQTKEHAFLARTLGIGHIIVAINKMDMVDYDEKKFKQVSE QVKKLLMMLGYKDFPIIPISAWEGDNVVKKSDKMPWYNGPTLIEALDQIPEPPKPTDKPLRIPIQDVYSIKGVGTVPVGR VETGVLRVGDVVIFEPASTIFHKPIQGEVKSIEMHHEPMQEALPGDNIGFNVRGVGKNDIKRGDVAGHTNNPPTVVRPKD TFKAQIIVLNHPTAITIGYTPVLHAHTLQVAVRFEQLLAKLDPRTGNVVEENPQFIKTGDSAIVVLRPTKPMVIEPVKEI PQMGRFAIRDMGQTVAAGMVISIQKAE
Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]
COG id: COG5256
COG function: function code J; Translation elongation factor EF-1alpha (GTPase)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]
Homologues:
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=51.6556291390728, Blast_Score=460, Evalue=1e-129, Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=51.6629711751663, Blast_Score=460, Evalue=1e-129, Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=37.1559633027523, Blast_Score=304, Evalue=1e-82, Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=37.1559633027523, Blast_Score=304, Evalue=1e-82, Organism=Homo sapiens, GI194097354, Length=437, Percent_Identity=36.8421052631579, Blast_Score=283, Evalue=3e-76, Organism=Homo sapiens, GI194018522, Length=437, Percent_Identity=36.8421052631579, Blast_Score=283, Evalue=3e-76, Organism=Homo sapiens, GI194018520, Length=437, Percent_Identity=36.8421052631579, Blast_Score=283, Evalue=3e-76, Organism=Homo sapiens, GI46094014, Length=437, Percent_Identity=36.6132723112128, Blast_Score=281, Evalue=6e-76, Organism=Homo sapiens, GI34147630, Length=432, Percent_Identity=33.7962962962963, Blast_Score=216, Evalue=3e-56, Organism=Homo sapiens, GI54607086, Length=336, Percent_Identity=26.4880952380952, Blast_Score=113, Evalue=4e-25, Organism=Homo sapiens, GI82546879, Length=423, Percent_Identity=24.3498817966903, Blast_Score=91, Evalue=2e-18, Organism=Homo sapiens, GI4503507, Length=324, Percent_Identity=26.8518518518519, Blast_Score=84, Evalue=3e-16, Organism=Homo sapiens, GI157426893, Length=303, Percent_Identity=27.7227722772277, Blast_Score=83, Evalue=5e-16, Organism=Homo sapiens, GI169162126, Length=145, Percent_Identity=33.7931034482759, Blast_Score=76, Evalue=6e-14, Organism=Homo sapiens, GI239752724, Length=145, Percent_Identity=33.7931034482759, Blast_Score=76, Evalue=6e-14, Organism=Homo sapiens, GI169162824, Length=145, Percent_Identity=33.7931034482759, Blast_Score=76, Evalue=6e-14, Organism=Homo sapiens, GI53729339, Length=247, Percent_Identity=28.3400809716599, Blast_Score=70, Evalue=5e-12, Organism=Homo sapiens, GI53729337, Length=247, Percent_Identity=28.3400809716599, Blast_Score=70, Evalue=5e-12, Organism=Escherichia coli, GI1789737, Length=429, Percent_Identity=35.6643356643357, Blast_Score=210, Evalue=1e-55, Organism=Escherichia coli, GI1790412, Length=429, Percent_Identity=35.6643356643357, Blast_Score=210, Evalue=1e-55, Organism=Escherichia coli, GI1789108, Length=314, Percent_Identity=38.5350318471338, Blast_Score=205, Evalue=4e-54, Organism=Escherichia coli, GI2367247, Length=298, Percent_Identity=31.5436241610738, Blast_Score=130, Evalue=1e-31, Organism=Escherichia coli, GI48994988, Length=245, Percent_Identity=33.0612244897959, Blast_Score=92, Evalue=6e-20, Organism=Escherichia coli, GI1789738, Length=178, Percent_Identity=34.8314606741573, Blast_Score=70, Evalue=4e-13, Organism=Escherichia coli, GI1788922, Length=252, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=9e-12, Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=51.6556291390728, Blast_Score=457, Evalue=1e-129, Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=51.6556291390728, Blast_Score=457, Evalue=1e-129, Organism=Caenorhabditis elegans, GI32566303, Length=446, Percent_Identity=49.3273542600897, Blast_Score=421, Evalue=1e-118, Organism=Caenorhabditis elegans, GI115532067, Length=429, Percent_Identity=36.3636363636364, Blast_Score=277, Evalue=8e-75, Organism=Caenorhabditis elegans, GI115532065, Length=429, Percent_Identity=36.3636363636364, Blast_Score=276, Evalue=2e-74, Organism=Caenorhabditis elegans, GI32566629, Length=437, Percent_Identity=35.4691075514874, Blast_Score=275, Evalue=4e-74, Organism=Caenorhabditis elegans, GI25141371, Length=362, Percent_Identity=35.3591160220994, Blast_Score=191, Evalue=7e-49, Organism=Caenorhabditis elegans, GI17556456, Length=436, Percent_Identity=33.4862385321101, Blast_Score=181, Evalue=5e-46, Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=61.744966442953, Blast_Score=177, Evalue=7e-45, Organism=Caenorhabditis elegans, GI17565438, Length=365, Percent_Identity=25.7534246575342, Blast_Score=94, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17557151, Length=244, Percent_Identity=31.9672131147541, Blast_Score=88, Evalue=8e-18, Organism=Caenorhabditis elegans, GI17506081, Length=363, Percent_Identity=22.8650137741047, Blast_Score=86, Evalue=4e-17, Organism=Caenorhabditis elegans, GI17509919, Length=342, Percent_Identity=24.8538011695906, Blast_Score=79, Evalue=4e-15, Organism=Caenorhabditis elegans, GI32566299, Length=64, Percent_Identity=59.375, Blast_Score=75, Evalue=8e-14, Organism=Saccharomyces cerevisiae, GI6325337, Length=453, Percent_Identity=51.4348785871965, Blast_Score=470, Evalue=1e-133, Organism=Saccharomyces cerevisiae, GI6319594, Length=453, Percent_Identity=51.4348785871965, Blast_Score=470, Evalue=1e-133, Organism=Saccharomyces cerevisiae, GI6320377, Length=439, Percent_Identity=35.7630979498861, Blast_Score=248, Evalue=9e-67, Organism=Saccharomyces cerevisiae, GI6324761, Length=437, Percent_Identity=35.0114416475973, Blast_Score=218, Evalue=1e-57, Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=30.989010989011, Blast_Score=206, Evalue=6e-54, Organism=Saccharomyces cerevisiae, GI6323320, Length=254, Percent_Identity=31.8897637795276, Blast_Score=87, Evalue=5e-18, Organism=Saccharomyces cerevisiae, GI6320863, Length=348, Percent_Identity=23.5632183908046, Blast_Score=75, Evalue=2e-14, Organism=Saccharomyces cerevisiae, GI6323098, Length=215, Percent_Identity=30.6976744186047, Blast_Score=67, Evalue=6e-12, Organism=Saccharomyces cerevisiae, GI6324550, Length=196, Percent_Identity=30.6122448979592, Blast_Score=64, Evalue=5e-11, Organism=Drosophila melanogaster, GI24652838, Length=451, Percent_Identity=52.9933481152993, Blast_Score=464, Evalue=1e-131, Organism=Drosophila melanogaster, GI17137572, Length=451, Percent_Identity=52.9933481152993, Blast_Score=464, Evalue=1e-131, Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=52.4229074889868, Blast_Score=460, Evalue=1e-130, Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=52.4229074889868, Blast_Score=460, Evalue=1e-130, Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=52.4229074889868, Blast_Score=460, Evalue=1e-130, Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=52.4229074889868, Blast_Score=460, Evalue=1e-130, Organism=Drosophila melanogaster, GI45550900, Length=439, Percent_Identity=36.9020501138952, Blast_Score=296, Evalue=2e-80, Organism=Drosophila melanogaster, GI17137380, Length=437, Percent_Identity=34.7826086956522, Blast_Score=270, Evalue=1e-72, Organism=Drosophila melanogaster, GI281363316, Length=430, Percent_Identity=36.7441860465116, Blast_Score=236, Evalue=3e-62, Organism=Drosophila melanogaster, GI17864358, Length=430, Percent_Identity=36.7441860465116, Blast_Score=236, Evalue=3e-62, Organism=Drosophila melanogaster, GI19921738, Length=427, Percent_Identity=30.4449648711944, Blast_Score=179, Evalue=3e-45, Organism=Drosophila melanogaster, GI24647046, Length=324, Percent_Identity=26.2345679012346, Blast_Score=90, Evalue=3e-18, Organism=Drosophila melanogaster, GI24647048, Length=324, Percent_Identity=25.9259259259259, Blast_Score=90, Evalue=4e-18, Organism=Drosophila melanogaster, GI19922690, Length=313, Percent_Identity=26.8370607028754, Blast_Score=87, Evalue=2e-17, Organism=Drosophila melanogaster, GI78706572, Length=308, Percent_Identity=26.9480519480519, Blast_Score=78, Evalue=9e-15, Organism=Drosophila melanogaster, GI28572034, Length=287, Percent_Identity=31.0104529616725, Blast_Score=69, Evalue=7e-12, Organism=Drosophila melanogaster, GI24582462, Length=206, Percent_Identity=30.5825242718447, Blast_Score=66, Evalue=4e-11,
Paralogues:
None
Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000795 - InterPro: IPR005225 - InterPro: IPR009001 - InterPro: IPR004539 - InterPro: IPR004161 - InterPro: IPR004160 - InterPro: IPR009000 [H]
Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]
EC number: NA
Molecular weight: Translated: 47501; Mature: 47370
Theoretical pI: Translated: 9.11; Mature: 9.11
Prosite motif: PS00301 EFACTOR_GTP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 3.7 %Met (Translated Protein) 3.7 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 3.5 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAKEKPHVNIVFIGHVDHGKSTTIGRLLFDTANIPENIIKKFEEMGEKGKSFKFAWVMDR CCCCCCCEEEEEEEECCCCCCCCHHHHEECCCCCCHHHHHHHHHHCCCCCCEEHHHHHHH LKEERERGITIDVAHTKFETPHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGV HHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEECCCC MPQTKEHAFLARTLGIGHIIVAINKMDMVDYDEKKFKQVSEQVKKLLMMLGYKDFPIIPI CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE SAWEGDNVVKKSDKMPWYNGPTLIEALDQIPEPPKPTDKPLRIPIQDVYSIKGVGTVPVG ECCCCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEECCHHHHHEECCCCCCCCC RVETGVLRVGDVVIFEPASTIFHKPIQGEVKSIEMHHEPMQEALPGDNIGFNVRGVGKND CCCCCEEEECCEEEECCCHHHHCCCCCCCHHEEEHHHHHHHHHCCCCCCCEEEEECCCCC IKRGDVAGHTNNPPTVVRPKDTFKAQIIVLNHPTAITIGYTPVLHAHTLQVAVRFEQLLA CCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHH KLDPRTGNVVEENPQFIKTGDSAIVVLRPTKPMVIEPVKEIPQMGRFAIRDMGQTVAAGM HCCCCCCCEECCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCE VISIQKAE EEEEEECC >Mature Secondary Structure AKEKPHVNIVFIGHVDHGKSTTIGRLLFDTANIPENIIKKFEEMGEKGKSFKFAWVMDR CCCCCCEEEEEEEECCCCCCCCHHHHEECCCCCCHHHHHHHHHHCCCCCCEEHHHHHHH LKEERERGITIDVAHTKFETPHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGV HHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEECCCC MPQTKEHAFLARTLGIGHIIVAINKMDMVDYDEKKFKQVSEQVKKLLMMLGYKDFPIIPI CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE SAWEGDNVVKKSDKMPWYNGPTLIEALDQIPEPPKPTDKPLRIPIQDVYSIKGVGTVPVG ECCCCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEECCHHHHHEECCCCCCCCC RVETGVLRVGDVVIFEPASTIFHKPIQGEVKSIEMHHEPMQEALPGDNIGFNVRGVGKND CCCCCEEEECCEEEECCCHHHHCCCCCCCHHEEEHHHHHHHHHCCCCCCCEEEEECCCCC IKRGDVAGHTNNPPTVVRPKDTFKAQIIVLNHPTAITIGYTPVLHAHTLQVAVRFEQLLA CCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHH KLDPRTGNVVEENPQFIKTGDSAIVVLRPTKPMVIEPVKEIPQMGRFAIRDMGQTVAAGM HCCCCCCCEECCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCE VISIQKAE EEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2115672 [H]