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The map label for this gene is rsmF [H]

Identifier: 212223865

GI number: 212223865

Start: 669413

End: 670351

Strand: Reverse

Name: rsmF [H]

Synonym: TON_0716

Alternate gene names: 212223865

Gene position: 670351-669413 (Counterclockwise)

Preceding gene: 212223866

Following gene: 212223864

Centisome position: 36.28

GC content: 53.57

Gene sequence:

>939_bases
ATGAGCGTGAGGGACGAGATCAGGGAGGCCAACCAGGAGTTTTACGAGCGCTATTCCCAGATAGAGGATACTGACGAGTT
TTGGGAGTTCATAGTCAAGCCGCTCCGGCAGAGCATAAGGGTGAACACGCTCAAGGCCCCGCTTGAGGTTGTAGTTGAAC
GCCTGAGTGAGGATTACAAGCTCGAGCCGATCCCATGGGTCAGGGAGGGCTTCTTTATCAACGTAGATAACCTCGCCAGA
GTTCCGGAGCACAGTCTGGGTCTGGTCTTCGGGCAAGAAGCCAGCTCAATGATTCCGCCAGTTGTTCTTGACCCAAAGCC
TGGAGAGCTCGTTCTCGACATGGCCGCCGCGCCGGGCTCCAAAACCGGGCAGATAGCACAGTACATGGAAAACGAGGGTT
GCATAATAGCCAATGACCCCAAGATAGACAGGGCAAATATACTCATAGCGAACCTCAACAGGATGGGCGTCTTGAACACC
CGCGTGACGACGCGCGATGGTGCATACTTCGGAAGGTTTGAGAATACCTTCGACAGAGTTCTACTCGATGCCCCCTGCTC
AAGCGTTGGCATGATAAGAAAGAGCTGGAAGTTCCTGACCGGATGGAGGCTCAGGGGAGTCGTCAAATACATGAACATCC
AGAAGAGGCTTATCCTTGCAGCATACAAAGCCCTCAAACCAGGTGGAGTGCTCGTTTACTCCACCTGCACCATAGACCCG
CTGGAGAACGAGGAGGTGGTTGACTACCTCCTGAGGAAGACGGACGCGAGGCTGGATGATATAGACCTGCCTGTGAAGAC
AAGCGAACCCGTTCTCGAGTGGGAGGGTAGAACCTACTCAGAAGAGCTTAGGAAAACCCTCAGGATTCATCCCAACGACA
ACGACACTGAAGCATTCTTCATTGCGAAGATAGTGAAGCCCGGGGAGGGAGAAGCATGA

Upstream 100 bases:

>100_bases
GGAAGAGGTCAAGGCCATAACCTACCACGACATGAGGATTGAAAAACTGTCAGACGGGAGATGGATGGTCCAGCTCGTGC
CCGATTTATGAGGTGGTCAG

Downstream 100 bases:

>100_bases
GCGAGAACCCTAGGGGAGAGATAGGGAAGACGGACGATACCGAGCTTGTGAAGAAGCTTTTACTTGAGAATTATGGCTAC
GCCCCGGAGTTAATCTACGA

Product: tRNA/rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSVRDEIREANQEFYERYSQIEDTDEFWEFIVKPLRQSIRVNTLKAPLEVVVERLSEDYKLEPIPWVREGFFINVDNLAR
VPEHSLGLVFGQEASSMIPPVVLDPKPGELVLDMAAAPGSKTGQIAQYMENEGCIIANDPKIDRANILIANLNRMGVLNT
RVTTRDGAYFGRFENTFDRVLLDAPCSSVGMIRKSWKFLTGWRLRGVVKYMNIQKRLILAAYKALKPGGVLVYSTCTIDP
LENEEVVDYLLRKTDARLDDIDLPVKTSEPVLEWEGRTYSEELRKTLRIHPNDNDTEAFFIAKIVKPGEGEA

Sequences:

>Translated_312_residues
MSVRDEIREANQEFYERYSQIEDTDEFWEFIVKPLRQSIRVNTLKAPLEVVVERLSEDYKLEPIPWVREGFFINVDNLAR
VPEHSLGLVFGQEASSMIPPVVLDPKPGELVLDMAAAPGSKTGQIAQYMENEGCIIANDPKIDRANILIANLNRMGVLNT
RVTTRDGAYFGRFENTFDRVLLDAPCSSVGMIRKSWKFLTGWRLRGVVKYMNIQKRLILAAYKALKPGGVLVYSTCTIDP
LENEEVVDYLLRKTDARLDDIDLPVKTSEPVLEWEGRTYSEELRKTLRIHPNDNDTEAFFIAKIVKPGEGEA
>Mature_311_residues
SVRDEIREANQEFYERYSQIEDTDEFWEFIVKPLRQSIRVNTLKAPLEVVVERLSEDYKLEPIPWVREGFFINVDNLARV
PEHSLGLVFGQEASSMIPPVVLDPKPGELVLDMAAAPGSKTGQIAQYMENEGCIIANDPKIDRANILIANLNRMGVLNTR
VTTRDGAYFGRFENTFDRVLLDAPCSSVGMIRKSWKFLTGWRLRGVVKYMNIQKRLILAAYKALKPGGVLVYSTCTIDPL
ENEEVVDYLLRKTDARLDDIDLPVKTSEPVLEWEGRTYSEELRKTLRIHPNDNDTEAFFIAKIVKPGEGEA

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=289, Percent_Identity=37.3702422145329, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI76150623, Length=289, Percent_Identity=37.3702422145329, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI39995082, Length=391, Percent_Identity=26.5984654731458, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI301336155, Length=360, Percent_Identity=26.1111111111111, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI32698918, Length=259, Percent_Identity=32.4324324324324, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI40316918, Length=207, Percent_Identity=32.8502415458937, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI11545785, Length=188, Percent_Identity=32.9787234042553, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI87081985, Length=292, Percent_Identity=40.4109589041096, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI2367212, Length=234, Percent_Identity=32.9059829059829, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17536757, Length=290, Percent_Identity=34.4827586206897, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25143471, Length=384, Percent_Identity=29.1666666666667, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115534021, Length=222, Percent_Identity=30.6306306306306, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71998419, Length=222, Percent_Identity=30.6306306306306, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=294, Percent_Identity=36.734693877551, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6319447, Length=268, Percent_Identity=34.7014925373134, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI22024126, Length=286, Percent_Identity=34.965034965035, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21355201, Length=319, Percent_Identity=29.4670846394984, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356513, Length=232, Percent_Identity=34.4827586206897, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24668781, Length=232, Percent_Identity=34.4827586206897, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19920494, Length=174, Percent_Identity=28.1609195402299, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 35556; Mature: 35425

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRDEIREANQEFYERYSQIEDTDEFWEFIVKPLRQSIRVNTLKAPLEVVVERLSEDYK
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEPIPWVREGFFINVDNLARVPEHSLGLVFGQEASSMIPPVVLDPKPGELVLDMAAAPGS
CCCCCHHHCCEEEEHHHHHHCCCCCCCEEECCCHHHCCCCEEECCCCCCEEEEEECCCCC
KTGQIAQYMENEGCIIANDPKIDRANILIANLNRMGVLNTRVTTRDGAYFGRFENTFDRV
CCHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCEEEECEEEECCCCEECCHHHHHHHH
LLDAPCSSVGMIRKSWKFLTGWRLRGVVKYMNIQKRLILAAYKALKPGGVLVYSTCTIDP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
LENEEVVDYLLRKTDARLDDIDLPVKTSEPVLEWEGRTYSEELRKTLRIHPNDNDTEAFF
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHEEECCCCCCCCEEE
IAKIVKPGEGEA
EEEEECCCCCCC
>Mature Secondary Structure 
SVRDEIREANQEFYERYSQIEDTDEFWEFIVKPLRQSIRVNTLKAPLEVVVERLSEDYK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEPIPWVREGFFINVDNLARVPEHSLGLVFGQEASSMIPPVVLDPKPGELVLDMAAAPGS
CCCCCHHHCCEEEEHHHHHHCCCCCCCEEECCCHHHCCCCEEECCCCCCEEEEEECCCCC
KTGQIAQYMENEGCIIANDPKIDRANILIANLNRMGVLNTRVTTRDGAYFGRFENTFDRV
CCHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCEEEECEEEECCCCEECCHHHHHHHH
LLDAPCSSVGMIRKSWKFLTGWRLRGVVKYMNIQKRLILAAYKALKPGGVLVYSTCTIDP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
LENEEVVDYLLRKTDARLDDIDLPVKTSEPVLEWEGRTYSEELRKTLRIHPNDNDTEAFF
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHEEECCCCCCCCEEE
IAKIVKPGEGEA
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA