Click here to switch to the map view.

The map label for this gene is cobQ

Identifier: 212223836

GI number: 212223836

Start: 639465

End: 640919

Strand: Reverse

Name: cobQ

Synonym: TON_0687

Alternate gene names: 212223836

Gene position: 640919-639465 (Counterclockwise)

Preceding gene: 212223838

Following gene: 212223834

Centisome position: 34.69

GC content: 52.3

Gene sequence:

>1455_bases
ATGGGAAAAGCCCTAATGGTTCAGGGAACTTCCTCCGGAGCTGGGAAATCCCTCCTCGTTATGGCTTTGTGCAGGATCTT
TTCGAACTTAGGGTATGACGTTGTTCCGTTTAAAAGCCAGAACATGAGCCTGAACTCCGCACCGAGCATAGAAGGCGGCG
AAATAAGTCGCGCCCAGTACCTTCAGGCGGTAGCCTGCAGAAAGAAGCCGTCGGTGAGGTTCAATCCAATACTCCTCAAG
CCTGAGGGGAACATGAGGAGCCAAGTTGTGTTTATGGGAAAGCCAATAGGAAGCGTTTCAGCTAGGGAATACATGCTCTC
CCGGAAGGAGGAGCTCTTTCGGAAGGCCATGAAGGTTCTGGATGAACTCATGGTGGAGCACGAGATTGTGATAATCGAAG
GCGCAGGCTCGCCCGTTGAGATTAACCTCAAGGACTACGACATAGCCAACATGCGCGTTGCGAGGCACGCCAAAGCGAAG
ACCATCCTCGTTACCGACATCGACCGAGGTGGGAGCTTCGCTTCTATAGTGGGTACGATGGAGCTCTTGAGCAAGGAGGA
GCGAAACTTGATCCTTGGCTTCGTATTCAATAAGTTCCGCGGCGATGCCTCCCTGCTGGAGCCAGGCTTCGAATATCTAG
AGAAGCGCTACGGGAAGCCTACCCTCGGAGTCGTTCCCTGCATCGAGCACAAGTTGCCAGAAGAGGACTCTTTGACTAGC
TTTCCGAAGGTTAATGGCGAGCTCCACATCCAGATAGTCAAGCTTCCCCACATAAGCAACTTTACCGACTTTGAGCCCCT
TCACTGGGCCAATGGGGTGGACTACGTTACAAAAGCGGAGGAAATCGAAGGTGATTTGATAATAATCCCCGGGAGCAAGA
ACACCGTCGAGGACTTGCTCTGGATGCGCGAGAATGGAATAGAGGACGCGATAATTCAGGCTCACCACGAAGGTTCTTTC
GTCGTTGGAATCTGCGGCGGCTTTCAAATGCTCGGGGAGAAGATAATCGACAACGTAGAATCGAAGCGCGGTGAGGTCAA
GGGTATTGGACTGTTGCCCGCTAAAACAATCTTCACGCCCGTTAAACGGACAAATCACCTGAAGGCCGAAATCCTTTGGG
AGCCGGCTAAGAGAATGAGCGTTGAGGGCTATGAGATAAGGATGGGCCGCTCAACCTCAGAGAGGCCCTTCTCGATAATA
CGCGAGATAAACGGTGCTAAGGCCTTTGAGCCGGAGGGAGCCCTTGGTGAGAGAACCTTCGGGACCTATCTACACGGCAT
CTTCCATAACTTCGCCTTTACGGAGCGTCTTCTCAACTTTCTGCGGGCGGAAAAAGGTCTCGAGCCGATAAGCGTTGGTG
GATGGAGCATTGAGGAGGAGATAGAGCGCTTTGCTAGGGTTGTTGAGAAGAACCTTGACGTGGGATACATCATCTCGGAG
CTCGGATTAGGTTAG

Upstream 100 bases:

>100_bases
TTTCCGAGGACAACCAGCATGAGGCTTTTCATAGTATCACCGATGGAGGTTTTATCCAACAGGTTTATATGTCCTTCGTC
CACTTCCAGTGGGGGTGTGA

Downstream 100 bases:

>100_bases
CAATCCTCTTCGCAAGCTCCAGTTCTTCAGGAGTGTTCACATTTAATGACAACAGGGGATTTGTGAACTCAAAGAATCTC
TCCCCTTCTTCAGCGACGGC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MGKALMVQGTSSGAGKSLLVMALCRIFSNLGYDVVPFKSQNMSLNSAPSIEGGEISRAQYLQAVACRKKPSVRFNPILLK
PEGNMRSQVVFMGKPIGSVSAREYMLSRKEELFRKAMKVLDELMVEHEIVIIEGAGSPVEINLKDYDIANMRVARHAKAK
TILVTDIDRGGSFASIVGTMELLSKEERNLILGFVFNKFRGDASLLEPGFEYLEKRYGKPTLGVVPCIEHKLPEEDSLTS
FPKVNGELHIQIVKLPHISNFTDFEPLHWANGVDYVTKAEEIEGDLIIIPGSKNTVEDLLWMRENGIEDAIIQAHHEGSF
VVGICGGFQMLGEKIIDNVESKRGEVKGIGLLPAKTIFTPVKRTNHLKAEILWEPAKRMSVEGYEIRMGRSTSERPFSII
REINGAKAFEPEGALGERTFGTYLHGIFHNFAFTERLLNFLRAEKGLEPISVGGWSIEEEIERFARVVEKNLDVGYIISE
LGLG

Sequences:

>Translated_484_residues
MGKALMVQGTSSGAGKSLLVMALCRIFSNLGYDVVPFKSQNMSLNSAPSIEGGEISRAQYLQAVACRKKPSVRFNPILLK
PEGNMRSQVVFMGKPIGSVSAREYMLSRKEELFRKAMKVLDELMVEHEIVIIEGAGSPVEINLKDYDIANMRVARHAKAK
TILVTDIDRGGSFASIVGTMELLSKEERNLILGFVFNKFRGDASLLEPGFEYLEKRYGKPTLGVVPCIEHKLPEEDSLTS
FPKVNGELHIQIVKLPHISNFTDFEPLHWANGVDYVTKAEEIEGDLIIIPGSKNTVEDLLWMRENGIEDAIIQAHHEGSF
VVGICGGFQMLGEKIIDNVESKRGEVKGIGLLPAKTIFTPVKRTNHLKAEILWEPAKRMSVEGYEIRMGRSTSERPFSII
REINGAKAFEPEGALGERTFGTYLHGIFHNFAFTERLLNFLRAEKGLEPISVGGWSIEEEIERFARVVEKNLDVGYIISE
LGLG
>Mature_483_residues
GKALMVQGTSSGAGKSLLVMALCRIFSNLGYDVVPFKSQNMSLNSAPSIEGGEISRAQYLQAVACRKKPSVRFNPILLKP
EGNMRSQVVFMGKPIGSVSAREYMLSRKEELFRKAMKVLDELMVEHEIVIIEGAGSPVEINLKDYDIANMRVARHAKAKT
ILVTDIDRGGSFASIVGTMELLSKEERNLILGFVFNKFRGDASLLEPGFEYLEKRYGKPTLGVVPCIEHKLPEEDSLTSF
PKVNGELHIQIVKLPHISNFTDFEPLHWANGVDYVTKAEEIEGDLIIIPGSKNTVEDLLWMRENGIEDAIIQAHHEGSFV
VGICGGFQMLGEKIIDNVESKRGEVKGIGLLPAKTIFTPVKRTNHLKAEILWEPAKRMSVEGYEIRMGRSTSERPFSIIR
EINGAKAFEPEGALGERTFGTYLHGIFHNFAFTERLLNFLRAEKGLEPISVGGWSIEEEIERFARVVEKNLDVGYIISEL
GLG

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_THEON (B6YV97)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002307072.1
- ProteinModelPortal:   B6YV97
- GeneID:   7016988
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0687
- HOGENOM:   HBG302271
- OMA:   ICLPRIS
- ProtClustDB:   PRK00784
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 53856; Mature: 53725

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 325-325 ACT_SITE 425-425

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKALMVQGTSSGAGKSLLVMALCRIFSNLGYDVVPFKSQNMSLNSAPSIEGGEISRAQY
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHH
LQAVACRKKPSVRFNPILLKPEGNMRSQVVFMGKPIGSVSAREYMLSRKEELFRKAMKVL
HHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
DELMVEHEIVIIEGAGSPVEINLKDYDIANMRVARHAKAKTILVTDIDRGGSFASIVGTM
HHHHHCCEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
ELLSKEERNLILGFVFNKFRGDASLLEPGFEYLEKRYGKPTLGVVPCIEHKLPEEDSLTS
HHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCC
FPKVNGELHIQIVKLPHISNFTDFEPLHWANGVDYVTKAEEIEGDLIIIPGSKNTVEDLL
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHH
WMRENGIEDAIIQAHHEGSFVVGICGGFQMLGEKIIDNVESKRGEVKGIGLLPAKTIFTP
HHHHCCCCHHHHEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
VKRTNHLKAEILWEPAKRMSVEGYEIRMGRSTSERPFSIIREINGAKAFEPEGALGERTF
HHHCCCCEEEEEECCHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHH
GTYLHGIFHNFAFTERLLNFLRAEKGLEPISVGGWSIEEEIERFARVVEKNLDVGYIISE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LGLG
CCCC
>Mature Secondary Structure 
GKALMVQGTSSGAGKSLLVMALCRIFSNLGYDVVPFKSQNMSLNSAPSIEGGEISRAQY
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHH
LQAVACRKKPSVRFNPILLKPEGNMRSQVVFMGKPIGSVSAREYMLSRKEELFRKAMKVL
HHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
DELMVEHEIVIIEGAGSPVEINLKDYDIANMRVARHAKAKTILVTDIDRGGSFASIVGTM
HHHHHCCEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
ELLSKEERNLILGFVFNKFRGDASLLEPGFEYLEKRYGKPTLGVVPCIEHKLPEEDSLTS
HHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCC
FPKVNGELHIQIVKLPHISNFTDFEPLHWANGVDYVTKAEEIEGDLIIIPGSKNTVEDLL
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHH
WMRENGIEDAIIQAHHEGSFVVGICGGFQMLGEKIIDNVESKRGEVKGIGLLPAKTIFTP
HHHHCCCCHHHHEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
VKRTNHLKAEILWEPAKRMSVEGYEIRMGRSTSERPFSIIREINGAKAFEPEGALGERTF
HHHCCCCEEEEEECCHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHH
GTYLHGIFHNFAFTERLLNFLRAEKGLEPISVGGWSIEEEIERFARVVEKNLDVGYIISE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LGLG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA