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The map label for this gene is 212223834

Identifier: 212223834

GI number: 212223834

Start: 637770

End: 638885

Strand: Reverse

Name: 212223834

Synonym: TON_0685

Alternate gene names: NA

Gene position: 638885-637770 (Counterclockwise)

Preceding gene: 212223836

Following gene: 212223829

Centisome position: 34.58

GC content: 48.66

Gene sequence:

>1116_bases
ATGCCAGAAGTTAAAAGATATATACCCATTGTGATTCTCGTTGCTGTGCTGCTGGTTGGGGGTTGTCTCCTAGACACTTA
TCAGGGTTACCAACCCAATGAAACAACCTCAAGTCCAGTACAGCCTTCTTACTTCAACGGGAACACAAACTCAACTTTCT
CCTCCCGTCCCTTAACATCAACTCGGGCCTTCTCCACTGAGACTATCATAAAAAACCTCAATCAAACAGAAGCCCCGACA
CCTTCATCTAATGAAACTGAAATCACTTCTAGTGTTTTTTTCAACGAATCACAGAATTTAACCTCTCTGATAATCAACAG
CTGGGCCTACTGGCTTCAGAACGCGAGTTCCCTGGTTATAGCAGAGAGTGGCTTTGATTTAATCGTCATGGACTACTCGC
GTGACGGAAGCGATGAAACTGCCTACAGCGGTGAGGAGATTGATCTGATTAAAAACGCGGGGGTTGTTCCGATAGCTTAC
ATCAGCATCGGCGAGGCTGAGGACTATCGCTTCTACTGGAAAGAGGAGTGGAAAGAAAAACCTCCTGCCTGGCTCGGACC
GGAGAATCCCAACTGGGAGGGGAACTACGCGGTAAAATACTGGGATGATGAGTGGAAGGAGATAATGTTTGAATATTTCG
ACAGGATCATTGCGCAAGGCTTTGCGGGGGTTTACCTTGATAAAGTTGACGAGTTCGAGTACTGGGGCGAGAATGGCTAT
GATGAGAACTGGACGGCCCGGAAGATGATCGAGTTCATACTCGAAATAGCAAACTACACGCGCTCGAAGGCCGGTGAGGA
TTTCCTGATAATCCCCCAAAACGGTGAGCAACTCCTTGAATATGATGACGGCAGACTTTTGAAGACGATTTCCGGCTGGG
CCAGTGAGGACGTCTTTTACGACGGCCTTAAGCCGAGCCCTTGGAGGGACGAGAAGGTGCCTTACTTGGACACGGTTGTT
AAGGCCGGAAAGCTTGTTTTAGTGGTTGACTACGTGGATGACAGAAGTAGTAGCGAAGAAAATCTCGTGAGGATCCTCGA
CTTCATCGAGAAGGCTCATGAAGGGGCTACATCCCCTACGGGGCAATGGAAGACAGGGAGCTGGACGAGCTCGTGA

Upstream 100 bases:

>100_bases
CCCATGCGGGTTGATTTGCCTCCGGCGAGGATGATTATCATAATCCCACCGTTTATAAGTGGGAGAAACCGTTAAAAGGT
CTTCTCTTTTACTTGGGCTT

Downstream 100 bases:

>100_bases
TTATCCCGGGTGTTCAGCCGCCCAAGTAAGCTCCCGCAAACGCCACAACCAAAAGCGTCCCAGCCCTCGTTATCTCCGCT
ATTGCACCTATGCAGTCGCC

Product: protein

Products: AMP; diphosphate; L-cysteinyl-tRNA(Cys)

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MPEVKRYIPIVILVAVLLVGGCLLDTYQGYQPNETTSSPVQPSYFNGNTNSTFSSRPLTSTRAFSTETIIKNLNQTEAPT
PSSNETEITSSVFFNESQNLTSLIINSWAYWLQNASSLVIAESGFDLIVMDYSRDGSDETAYSGEEIDLIKNAGVVPIAY
ISIGEAEDYRFYWKEEWKEKPPAWLGPENPNWEGNYAVKYWDDEWKEIMFEYFDRIIAQGFAGVYLDKVDEFEYWGENGY
DENWTARKMIEFILEIANYTRSKAGEDFLIIPQNGEQLLEYDDGRLLKTISGWASEDVFYDGLKPSPWRDEKVPYLDTVV
KAGKLVLVVDYVDDRSSSEENLVRILDFIEKAHEGATSPTGQWKTGSWTSS

Sequences:

>Translated_371_residues
MPEVKRYIPIVILVAVLLVGGCLLDTYQGYQPNETTSSPVQPSYFNGNTNSTFSSRPLTSTRAFSTETIIKNLNQTEAPT
PSSNETEITSSVFFNESQNLTSLIINSWAYWLQNASSLVIAESGFDLIVMDYSRDGSDETAYSGEEIDLIKNAGVVPIAY
ISIGEAEDYRFYWKEEWKEKPPAWLGPENPNWEGNYAVKYWDDEWKEIMFEYFDRIIAQGFAGVYLDKVDEFEYWGENGY
DENWTARKMIEFILEIANYTRSKAGEDFLIIPQNGEQLLEYDDGRLLKTISGWASEDVFYDGLKPSPWRDEKVPYLDTVV
KAGKLVLVVDYVDDRSSSEENLVRILDFIEKAHEGATSPTGQWKTGSWTSS
>Mature_370_residues
PEVKRYIPIVILVAVLLVGGCLLDTYQGYQPNETTSSPVQPSYFNGNTNSTFSSRPLTSTRAFSTETIIKNLNQTEAPTP
SSNETEITSSVFFNESQNLTSLIINSWAYWLQNASSLVIAESGFDLIVMDYSRDGSDETAYSGEEIDLIKNAGVVPIAYI
SIGEAEDYRFYWKEEWKEKPPAWLGPENPNWEGNYAVKYWDDEWKEIMFEYFDRIIAQGFAGVYLDKVDEFEYWGENGYD
ENWTARKMIEFILEIANYTRSKAGEDFLIIPQNGEQLLEYDDGRLLKTISGWASEDVFYDGLKPSPWRDEKVPYLDTVVK
AGKLVLVVDYVDDRSSSEENLVRILDFIEKAHEGATSPTGQWKTGSWTSS

Specific function: Unknown

COG id: COG2342

COG function: function code G; Predicted extracellular endo alpha-1,4 polygalactosaminidase or related polysaccharide hydrolase

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016063
- InterPro:   IPR017853
- InterPro:   IPR016062
- InterPro:   IPR004352 [H]

Pfam domain/function: PF03537 DUF297 [H]

EC number: 6.1.1.16

Molecular weight: Translated: 42266; Mature: 42135

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVKRYIPIVILVAVLLVGGCLLDTYQGYQPNETTSSPVQPSYFNGNTNSTFSSRPLTS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
TRAFSTETIIKNLNQTEAPTPSSNETEITSSVFFNESQNLTSLIINSWAYWLQNASSLVI
HHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHEECCCCCHHHHHHHHHHHHHCCCCEEEE
AESGFDLIVMDYSRDGSDETAYSGEEIDLIKNAGVVPIAYISIGEAEDYRFYWKEEWKEK
EECCCEEEEEECCCCCCCCCCCCCCCEEHHHCCCCCEEEEEEECCCCCCEEHHHHHHCCC
PPAWLGPENPNWEGNYAVKYWDDEWKEIMFEYFDRIIAQGFAGVYLDKVDEFEYWGENGY
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
DENWTARKMIEFILEIANYTRSKAGEDFLIIPQNGEQLLEYDDGRLLKTISGWASEDVFY
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCCHHHHHHCCCCCCCEE
DGLKPSPWRDEKVPYLDTVVKAGKLVLVVDYVDDRSSSEENLVRILDFIEKAHEGATSPT
CCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GQWKTGSWTSS
CCCCCCCCCCC
>Mature Secondary Structure 
PEVKRYIPIVILVAVLLVGGCLLDTYQGYQPNETTSSPVQPSYFNGNTNSTFSSRPLTS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
TRAFSTETIIKNLNQTEAPTPSSNETEITSSVFFNESQNLTSLIINSWAYWLQNASSLVI
HHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHEECCCCCHHHHHHHHHHHHHCCCCEEEE
AESGFDLIVMDYSRDGSDETAYSGEEIDLIKNAGVVPIAYISIGEAEDYRFYWKEEWKEK
EECCCEEEEEECCCCCCCCCCCCCCCEEHHHCCCCCEEEEEEECCCCCCEEHHHHHHCCC
PPAWLGPENPNWEGNYAVKYWDDEWKEIMFEYFDRIIAQGFAGVYLDKVDEFEYWGENGY
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
DENWTARKMIEFILEIANYTRSKAGEDFLIIPQNGEQLLEYDDGRLLKTISGWASEDVFY
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCCHHHHHHCCCCCCCEE
DGLKPSPWRDEKVPYLDTVVKAGKLVLVVDYVDDRSSSEENLVRILDFIEKAHEGATSPT
CCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GQWKTGSWTSS
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-cysteine; tRNA(Cys)

Specific reaction: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]