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The map label for this gene is ribE [H]

Identifier: 212223757

GI number: 212223757

Start: 561615

End: 562172

Strand: Reverse

Name: ribE [H]

Synonym: TON_0609

Alternate gene names: 212223757

Gene position: 562172-561615 (Counterclockwise)

Preceding gene: 212223758

Following gene: 212223756

Centisome position: 30.43

GC content: 53.05

Gene sequence:

>558_bases
GTGTTCAGCGGCATTATTGAGGCCATAGGCAAGGCGAGATACTCTGCTGGAAGGCTCCATGTCGAAGTCCCTTTCAAGGT
TACGCCGGGAGCTAGTGTGGCAGTAAATGGGGCCTGCCTGACGGTCATAAGCTTTGACGGAAGGATAGCCACCTTCGACG
TCGGCGAAGAAACTCGGAAGAGAACCAATCTGACTAGGGCAAAGTTTGTGAACCTTGAGCGGGCTATGAAGGTGGGCGAA
AGGCTTAATGGACACATAGTTACTGGCCACGTGGACGGCACGCTGAGATTTCTGGCATCGAGGAAAAGCAAAAACACGGC
ATGGATGGCCTTCGAGATGCCCCCCGAGCGGTGGGGAGTGGCTGAGAAGGGGAGCATAGCCATTAACGGTGTCTCCCTGA
CGGTTGCTAAGGTTGAGCCTACACGTTTCTGGGTTCAGGTGATACCCTACACGCTGAAGAAAACCAATCTCGGCTCGCTG
AGGCCAGGTGATGAAGTCAACTATGAGATAGATGTTCTAGCTAGATACGTGAGGAGAATCCAGGAGGTGAAAGGATGA

Upstream 100 bases:

>100_bases
CTCAGCGTGAAAGAAGCCAACGAGGCACCGGTGTTAGAGATAGAATCAATCGAGAAGCTCGGTGAGAGCCTCCTTGTTAC
AGCTTACCCGGGTGATTGTG

Downstream 100 bases:

>100_bases
AACCGGACTGGGAAAGCATCAGGAGGGCAATCATTGATGGAAAACCCGCTGTCCTTGTAGACAATAGGAGGGAGTTTGAA
GCTGATTTAGTTTATCCAGC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MFSGIIEAIGKARYSAGRLHVEVPFKVTPGASVAVNGACLTVISFDGRIATFDVGEETRKRTNLTRAKFVNLERAMKVGE
RLNGHIVTGHVDGTLRFLASRKSKNTAWMAFEMPPERWGVAEKGSIAINGVSLTVAKVEPTRFWVQVIPYTLKKTNLGSL
RPGDEVNYEIDVLARYVRRIQEVKG

Sequences:

>Translated_185_residues
MFSGIIEAIGKARYSAGRLHVEVPFKVTPGASVAVNGACLTVISFDGRIATFDVGEETRKRTNLTRAKFVNLERAMKVGE
RLNGHIVTGHVDGTLRFLASRKSKNTAWMAFEMPPERWGVAEKGSIAINGVSLTVAKVEPTRFWVQVIPYTLKKTNLGSL
RPGDEVNYEIDVLARYVRRIQEVKG
>Mature_185_residues
MFSGIIEAIGKARYSAGRLHVEVPFKVTPGASVAVNGACLTVISFDGRIATFDVGEETRKRTNLTRAKFVNLERAMKVGE
RLNGHIVTGHVDGTLRFLASRKSKNTAWMAFEMPPERWGVAEKGSIAINGVSLTVAKVEPTRFWVQVIPYTLKKTNLGSL
RPGDEVNYEIDVLARYVRRIQEVKG

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=185, Percent_Identity=38.9189189189189, Blast_Score=110, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319733, Length=203, Percent_Identity=31.0344827586207, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 20476; Mature: 20476

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGIIEAIGKARYSAGRLHVEVPFKVTPGASVAVNGACLTVISFDGRIATFDVGEETRK
CCHHHHHHHHHHCCCCCEEEEEECEEECCCCCEEECCEEEEEEEECCEEEEEECCHHHHH
RTNLTRAKFVNLERAMKVGERLNGHIVTGHVDGTLRFLASRKSKNTAWMAFEMPPERWGV
HHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHEEEEECCCCCCEEEEEEECCHHHCCC
AEKGSIAINGVSLTVAKVEPTRFWVQVIPYTLKKTNLGSLRPGDEVNYEIDVLARYVRRI
CCCCCEEEECEEEEEEEECCCEEEEEEECEEEECCCCCCCCCCCCCCEEHHHHHHHHHHH
QEVKG
HHHCC
>Mature Secondary Structure
MFSGIIEAIGKARYSAGRLHVEVPFKVTPGASVAVNGACLTVISFDGRIATFDVGEETRK
CCHHHHHHHHHHCCCCCEEEEEECEEECCCCCEEECCEEEEEEEECCEEEEEECCHHHHH
RTNLTRAKFVNLERAMKVGERLNGHIVTGHVDGTLRFLASRKSKNTAWMAFEMPPERWGV
HHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHEEEEECCCCCCEEEEEEECCHHHCCC
AEKGSIAINGVSLTVAKVEPTRFWVQVIPYTLKKTNLGSLRPGDEVNYEIDVLARYVRRI
CCCCCEEEECEEEEEEEECCCEEEEEEECEEEECCCCCCCCCCCCCCEEHHHHHHHHHHH
QEVKG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]