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The map label for this gene is asrB [H]

Identifier: 212223684

GI number: 212223684

Start: 490422

End: 491297

Strand: Reverse

Name: asrB [H]

Synonym: TON_0536

Alternate gene names: 212223684

Gene position: 491297-490422 (Counterclockwise)

Preceding gene: 212223685

Following gene: 212223683

Centisome position: 26.59

GC content: 52.63

Gene sequence:

>876_bases
ATGAACGAGGCTCACGTCTGCATGTGTCACGACAATCCCTACGCCCTTGACAGGGTCAAGGTTCTCAGAGTGTACCGTTT
AACAGAAACAGAGAAGCTATTTCTGTTCAGGTTTGAAGATCAAGAGATAGCCGAGAACTGGACCTTTAAGCCAGGACAGT
TCGTTCAGCTTACCATACCCGGTGTTGGCGAAGTCCCGATAAGCATCTGCTCGTCTCCCATGAAGAGAGGATTCTTTGAG
CTCTGTATCAGAAAGGCGGGAAGAGTCACCACAGTTGTTCACAAGCTCAAGCCGGGCGACACTGTCCTTGTCCGCGGGCC
GTACGGAAACGGCTTCCCTGTCGATGAGTGGGAAGGGATGGACCTACTCCTCATCGCCGCAGGATTAGGAACAGCCCCAC
TCAGGAGCGTCTTCCTCTACGCCATGGACAACCGCTGGAAGTACGGAAACATAACCTTCATCAACACCGCCCGTTACGGA
AAGGACCTCCTCTTCTACAAGGAACTTGAAGCCATGAAGGATCTCGCAGAGGCCGAGAACGTCCAGATAATTCAGAGCGT
AACCAGAGATCCTGACTGGCCCGGCAGACATGGAAGGCCTCAGAAGTTCATAGTCGAGGCCAATACCAACCCGAAGAACA
CCGCGATAGCTATCTGCGGTCCGCCGAGGATGTATAAGGCGGTCTTCGAGGCGCTCATCAATTACGGTTACCGCCCTGAG
AACATCTATGTAACGCTGGAAAGGAAAATGAAGTGTGGAATAGGCAAGTGCGGCCACTGTAACGTCGGAACGAGTACTAG
CTGGAAGTACGTCTGCAGAGATGGACCGGTTTTCACGTACTTCGACATAGTATCAACGCCGGGACTGCTGGACTGA

Upstream 100 bases:

>100_bases
TTATAGCTGGATTGGAGGATGCATCCTGCCCGTCAAAGCTGAGCGAGGAAATTCCAAAGAAAGGTTTTGCATATGCCAAC
AACATTAGAGGTGAAGACAT

Downstream 100 bases:

>100_bases
GGTGGTGGAGATGGAGGGGAAGGTTCGTATAGGGTTTTACGCTCTCACCTCATGCTACGGCTGTCAGCTCCGGTTCGCCA
TGATGGACGAGATACTTCAG

Product: cytochrome-c3 hydrogenase subunit gamma

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MNEAHVCMCHDNPYALDRVKVLRVYRLTETEKLFLFRFEDQEIAENWTFKPGQFVQLTIPGVGEVPISICSSPMKRGFFE
LCIRKAGRVTTVVHKLKPGDTVLVRGPYGNGFPVDEWEGMDLLLIAAGLGTAPLRSVFLYAMDNRWKYGNITFINTARYG
KDLLFYKELEAMKDLAEAENVQIIQSVTRDPDWPGRHGRPQKFIVEANTNPKNTAIAICGPPRMYKAVFEALINYGYRPE
NIYVTLERKMKCGIGKCGHCNVGTSTSWKYVCRDGPVFTYFDIVSTPGLLD

Sequences:

>Translated_291_residues
MNEAHVCMCHDNPYALDRVKVLRVYRLTETEKLFLFRFEDQEIAENWTFKPGQFVQLTIPGVGEVPISICSSPMKRGFFE
LCIRKAGRVTTVVHKLKPGDTVLVRGPYGNGFPVDEWEGMDLLLIAAGLGTAPLRSVFLYAMDNRWKYGNITFINTARYG
KDLLFYKELEAMKDLAEAENVQIIQSVTRDPDWPGRHGRPQKFIVEANTNPKNTAIAICGPPRMYKAVFEALINYGYRPE
NIYVTLERKMKCGIGKCGHCNVGTSTSWKYVCRDGPVFTYFDIVSTPGLLD
>Mature_291_residues
MNEAHVCMCHDNPYALDRVKVLRVYRLTETEKLFLFRFEDQEIAENWTFKPGQFVQLTIPGVGEVPISICSSPMKRGFFE
LCIRKAGRVTTVVHKLKPGDTVLVRGPYGNGFPVDEWEGMDLLLIAAGLGTAPLRSVFLYAMDNRWKYGNITFINTARYG
KDLLFYKELEAMKDLAEAENVQIIQSVTRDPDWPGRHGRPQKFIVEANTNPKNTAIAICGPPRMYKAVFEALINYGYRPE
NIYVTLERKMKCGIGKCGHCNVGTSTSWKYVCRDGPVFTYFDIVSTPGLLD

Specific function: This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322146, Length=170, Percent_Identity=27.6470588235294, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- InterPro:   IPR014260 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 33011; Mature: 33011

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAHVCMCHDNPYALDRVKVLRVYRLTETEKLFLFRFEDQEIAENWTFKPGQFVQLTIP
CCCCEEEEECCCCCCHHHHEEEHEEEECCCCEEEEEEECCHHHHHCCCCCCCCEEEEECC
GVGEVPISICSSPMKRGFFELCIRKAGRVTTVVHKLKPGDTVLVRGPYGNGFPVDEWEGM
CCCCCHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
DLLLIAAGLGTAPLRSVFLYAMDNRWKYGNITFINTARYGKDLLFYKELEAMKDLAEAEN
EEEEEECCCCCHHHHHEEEEEECCCEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
VQIIQSVTRDPDWPGRHGRPQKFIVEANTNPKNTAIAICGPPRMYKAVFEALINYGYRPE
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCC
NIYVTLERKMKCGIGKCGHCNVGTSTSWKYVCRDGPVFTYFDIVSTPGLLD
EEEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MNEAHVCMCHDNPYALDRVKVLRVYRLTETEKLFLFRFEDQEIAENWTFKPGQFVQLTIP
CCCCEEEEECCCCCCHHHHEEEHEEEECCCCEEEEEEECCHHHHHCCCCCCCCEEEEECC
GVGEVPISICSSPMKRGFFELCIRKAGRVTTVVHKLKPGDTVLVRGPYGNGFPVDEWEGM
CCCCCHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
DLLLIAAGLGTAPLRSVFLYAMDNRWKYGNITFINTARYGKDLLFYKELEAMKDLAEAEN
EEEEEECCCCCHHHHHEEEEEECCCEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
VQIIQSVTRDPDWPGRHGRPQKFIVEANTNPKNTAIAICGPPRMYKAVFEALINYGYRPE
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCC
NIYVTLERKMKCGIGKCGHCNVGTSTSWKYVCRDGPVFTYFDIVSTPGLLD
EEEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1704886; 11677609 [H]