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The map label for this gene is mvhA [H]

Identifier: 212223682

GI number: 212223682

Start: 488328

End: 489617

Strand: Reverse

Name: mvhA [H]

Synonym: TON_0534

Alternate gene names: 212223682

Gene position: 489617-488328 (Counterclockwise)

Preceding gene: 212223683

Following gene: 212223681

Centisome position: 26.5

GC content: 55.19

Gene sequence:

>1290_bases
ATGAAGAACCTCTACCTTCCAATCACCGTTGATCACATAGCGCGTGTAGAGGGCAAGGGCGGCGTTGAAATAGTCGTTGG
TGATGACGGGGTCAAGGAGGTCAAGCTCAACATCATTGAGGGTCCGAGGTTCTTTGAGGCGATAACCATAGGCAAGAAGC
TCGACGAGGCTTTGGCCGTTTATCCTAGGATATGCTCCTTCTGTTCAGCAGCCCACAAGCTCACTGCCGTCGAGGCGGCT
GAAAAGGCAGTTGGCTTTGAGGTACGCGAGGAGATACAGGCCCTCAGGGAGGTTCTCTACATTGGAGACATGATAGAGAG
CCACGCGCTCCACCTCTACCTCCTCGTCCTGCCAGATTACATGGGCTACTCCAACCCGCTCAAAATGCTTGACAAGTACA
AGAAAGAGATCAACATTGCCCTTGACCTTAAGAACCTCGGAAGCTGGATGATGGACGAGCTTGGTGCAAGGGCCATCCAC
CAGGAAAACGTCGTCATGGGTGGTTTTGGCAAGCTCCCCGACAAGACTACGCTGGAGAAGATGAAGAAGAGGCTCCAGGA
GGCCCTTCCACTTGCAGAATACACCTTCGAGCTGTTCTCCAAGCTTGAGCAGTACGAGGAAGTCGAGGGGCCGATAATCC
ATATGGCCGTCAGGCCGAGGGGAGACGTCTACGGCATATACGGCGACGCCATAAGCGTCAGCGACGGTTTCGAGTTCCCG
AGCGAGGGCTACAAGAAGCACATGGTTGAGCGCGTTGTGGAGCACAGCTTCGCCAAGCACAGCTTCTACAACGGGGAGAA
GCCCTTCATGACGGGAGCCATATCGCGCGTTGTCAACCATGCTGATAAGCTCTACGGAAGGGCAAAGGAGCTCTACGAGA
GCCACAAGGACTTACTCAGGCCAACCAACCCCTTCGCCAACAACCTCGCCCAGGCGCTTGAGTTAGTGTACTTCATGGAG
AGGGGCATCGACCTTATCGACGAGGCCCTCGCTAAATGGCCGATAAGGCCGAGGGACGAGGTAGATGTAAAGGACGGCTT
CGGTGTCAGCACCACCGAGGCACCGCGTGGAATCCTCGTCTACGCCCTAGAAGTCAAGGACGGAAGAGTAGCTTACGCGG
ATATCATAACGCCCACAGCCTTCAATCTCGCCATGATGGAGGTTCATGTACGCATGATGGCTGAGAAGCACTACAACGAC
GATCCTGAGAGGCTGAAGCTCCTTGCTGAGATGGTCGTCAGGGCCTACGACCCGTGCATCTCCTGTTCGGTGCACGTGGC
GAGGCTTTAG

Upstream 100 bases:

>100_bases
CCAAGGAAGAAATCCTCGAAAGAATGAAGATTTTCAACGCACACAATCCAAAGCTGGAGGAGATGGTCGATAAGGTCTTT
CAGTTTCAGGGGGTGAAAGA

Downstream 100 bases:

>100_bases
CCATATCTTTTTATTCCCCTCTCAGATTCTTCTTTGGAGGCGTATTCATGGAGACCCTAATCCTCGCCCTCGGCAACGAA
CTTATGAAGGATGATGGTGT

Product: cytosolic NiFe-hydrogenase, alpha subunit

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: Methyl viologen-reducing hydrogenase subunit alpha; MVH subunit A [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKNLYLPITVDHIARVEGKGGVEIVVGDDGVKEVKLNIIEGPRFFEAITIGKKLDEALAVYPRICSFCSAAHKLTAVEAA
EKAVGFEVREEIQALREVLYIGDMIESHALHLYLLVLPDYMGYSNPLKMLDKYKKEINIALDLKNLGSWMMDELGARAIH
QENVVMGGFGKLPDKTTLEKMKKRLQEALPLAEYTFELFSKLEQYEEVEGPIIHMAVRPRGDVYGIYGDAISVSDGFEFP
SEGYKKHMVERVVEHSFAKHSFYNGEKPFMTGAISRVVNHADKLYGRAKELYESHKDLLRPTNPFANNLAQALELVYFME
RGIDLIDEALAKWPIRPRDEVDVKDGFGVSTTEAPRGILVYALEVKDGRVAYADIITPTAFNLAMMEVHVRMMAEKHYND
DPERLKLLAEMVVRAYDPCISCSVHVARL

Sequences:

>Translated_429_residues
MKNLYLPITVDHIARVEGKGGVEIVVGDDGVKEVKLNIIEGPRFFEAITIGKKLDEALAVYPRICSFCSAAHKLTAVEAA
EKAVGFEVREEIQALREVLYIGDMIESHALHLYLLVLPDYMGYSNPLKMLDKYKKEINIALDLKNLGSWMMDELGARAIH
QENVVMGGFGKLPDKTTLEKMKKRLQEALPLAEYTFELFSKLEQYEEVEGPIIHMAVRPRGDVYGIYGDAISVSDGFEFP
SEGYKKHMVERVVEHSFAKHSFYNGEKPFMTGAISRVVNHADKLYGRAKELYESHKDLLRPTNPFANNLAQALELVYFME
RGIDLIDEALAKWPIRPRDEVDVKDGFGVSTTEAPRGILVYALEVKDGRVAYADIITPTAFNLAMMEVHVRMMAEKHYND
DPERLKLLAEMVVRAYDPCISCSVHVARL
>Mature_429_residues
MKNLYLPITVDHIARVEGKGGVEIVVGDDGVKEVKLNIIEGPRFFEAITIGKKLDEALAVYPRICSFCSAAHKLTAVEAA
EKAVGFEVREEIQALREVLYIGDMIESHALHLYLLVLPDYMGYSNPLKMLDKYKKEINIALDLKNLGSWMMDELGARAIH
QENVVMGGFGKLPDKTTLEKMKKRLQEALPLAEYTFELFSKLEQYEEVEGPIIHMAVRPRGDVYGIYGDAISVSDGFEFP
SEGYKKHMVERVVEHSFAKHSFYNGEKPFMTGAISRVVNHADKLYGRAKELYESHKDLLRPTNPFANNLAQALELVYFME
RGIDLIDEALAKWPIRPRDEVDVKDGFGVSTTEAPRGILVYALEVKDGRVAYADIITPTAFNLAMMEVHVRMMAEKHYND
DPERLKLLAEMVVRAYDPCISCSVHVARL

Specific function: Part of a complex that provides reducing equivalents for heterodisulfide reductase [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 48447; Mature: 48447

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLYLPITVDHIARVEGKGGVEIVVGDDGVKEVKLNIIEGPRFFEAITIGKKLDEALAV
CCCEEEEEEHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHH
YPRICSFCSAAHKLTAVEAAEKAVGFEVREEIQALREVLYIGDMIESHALHLYLLVLPDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHH
MGYSNPLKMLDKYKKEINIALDLKNLGSWMMDELGARAIHQENVVMGGFGKLPDKTTLEK
CCCCCHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHH
MKKRLQEALPLAEYTFELFSKLEQYEEVEGPIIHMAVRPRGDVYGIYGDAISVSDGFEFP
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCEEECCCCCCCC
SEGYKKHMVERVVEHSFAKHSFYNGEKPFMTGAISRVVNHADKLYGRAKELYESHKDLLR
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTNPFANNLAQALELVYFMERGIDLIDEALAKWPIRPRDEVDVKDGFGVSTTEAPRGILV
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
YALEVKDGRVAYADIITPTAFNLAMMEVHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCI
EEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHH
SCSVHVARL
CCCEEEECC
>Mature Secondary Structure
MKNLYLPITVDHIARVEGKGGVEIVVGDDGVKEVKLNIIEGPRFFEAITIGKKLDEALAV
CCCEEEEEEHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHH
YPRICSFCSAAHKLTAVEAAEKAVGFEVREEIQALREVLYIGDMIESHALHLYLLVLPDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHH
MGYSNPLKMLDKYKKEINIALDLKNLGSWMMDELGARAIHQENVVMGGFGKLPDKTTLEK
CCCCCHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHH
MKKRLQEALPLAEYTFELFSKLEQYEEVEGPIIHMAVRPRGDVYGIYGDAISVSDGFEFP
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCEEECCCCCCCC
SEGYKKHMVERVVEHSFAKHSFYNGEKPFMTGAISRVVNHADKLYGRAKELYESHKDLLR
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTNPFANNLAQALELVYFMERGIDLIDEALAKWPIRPRDEVDVKDGFGVSTTEAPRGILV
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
YALEVKDGRVAYADIITPTAFNLAMMEVHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCI
EEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHH
SCSVHVARL
CCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA