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The map label for this gene is thiL [H]

Identifier: 212223565

GI number: 212223565

Start: 391027

End: 391962

Strand: Reverse

Name: thiL [H]

Synonym: TON_0419

Alternate gene names: 212223565

Gene position: 391962-391027 (Counterclockwise)

Preceding gene: 212223567

Following gene: 212223564

Centisome position: 21.21

GC content: 50.64

Gene sequence:

>936_bases
ATGGAGCGCGAAATCATCGAACTCTTCATGAAATATCTCAAAAAGCAAGGTGATCTGCCACTCGGCGATGATGCAGGGGC
ACTGAGGATCGGTGATGAATGGCTGGTGGCGACAAATGACATGCTCGTGGGGAAGACAGACGTTCCGGATATAATGACGC
CCGAGCAGGTTGGCTTTAAGGCCGTGACGATGAACGTGAGTGACATCGCGGCTATGGGAGCGAGACCAATAGGCTTCCTT
TTCTCTTTAGGTGTTCCCCGAGGCTTTGATGGGTTATATCTTGAGGGAGTAGCGAAGGGAATAGGAAATGCCCTTGAGTT
CTACGAGATTCCCGTCCTAAGCGCCGATACCAACGAGGCAGATGATCTGATAATAGATGGAATTGCACTCGGAAGAACCA
GGCATCTGTTAACAAGGAGCGGAGCAAAGCCGGGAGACCTAGTCTGTATCACAGGGGATATCGGCAGGCCACTGGCAGGA
CTCCTCGTATGGAAGAACGATCTCGACGTTTCGCTCTCGACGAAAGAGATTCTATACGAGAAGCTCCTCGAACCGCGTGC
AAGAGTAAAGGAGGGGATTAAAATAGGAAATTACGCAAACGCTGCCATAGACATCAGTGACGGTCTTGCCAAGGAACTCC
ATCTCGTAGCAGAGATGAGCGGCGTTAGGATAGAGGTGGATGCCTCAAAGCTCCCCATAATGGAGGAGGTTTTTGAAGTG
GCCGAACTGCTCAGAAGGGATCCCCTTGAAATAGCCCTTGCAAGCGGGGAGGAGTTTGAGCTGGTTTTCACTATTCCTCC
GAAATACACTGAGGAACTGAGTTTCGACTTCACAGTCATAGGTACAGTCCATAAAGGGGCCGGAGTGTTCATTACCACTG
AGGAAAAACGGATGGAGGTTCCTCCTATAGGCTGGGAACACCTTGATAGCCGTTGA

Upstream 100 bases:

>100_bases
CAAATTTCATTTATAAATTTCTCGCTTTGTCTAGCAAACCTTCGACGGATGCCGAAAGCAATCCTAATAACTATCCCATC
TGAACCTTGGCGTGATGTCC

Downstream 100 bases:

>100_bases
AGTGGGCATCCTCGCTTTGTTCTTCTCCCTTACCCTCAGAACTCGGATAAGATTTATAAACTGTCATCGTGAGTAAAGGT
TGGCGAGAGCATGTTCGAGA

Product: thiamine monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MEREIIELFMKYLKKQGDLPLGDDAGALRIGDEWLVATNDMLVGKTDVPDIMTPEQVGFKAVTMNVSDIAAMGARPIGFL
FSLGVPRGFDGLYLEGVAKGIGNALEFYEIPVLSADTNEADDLIIDGIALGRTRHLLTRSGAKPGDLVCITGDIGRPLAG
LLVWKNDLDVSLSTKEILYEKLLEPRARVKEGIKIGNYANAAIDISDGLAKELHLVAEMSGVRIEVDASKLPIMEEVFEV
AELLRRDPLEIALASGEEFELVFTIPPKYTEELSFDFTVIGTVHKGAGVFITTEEKRMEVPPIGWEHLDSR

Sequences:

>Translated_311_residues
MEREIIELFMKYLKKQGDLPLGDDAGALRIGDEWLVATNDMLVGKTDVPDIMTPEQVGFKAVTMNVSDIAAMGARPIGFL
FSLGVPRGFDGLYLEGVAKGIGNALEFYEIPVLSADTNEADDLIIDGIALGRTRHLLTRSGAKPGDLVCITGDIGRPLAG
LLVWKNDLDVSLSTKEILYEKLLEPRARVKEGIKIGNYANAAIDISDGLAKELHLVAEMSGVRIEVDASKLPIMEEVFEV
AELLRRDPLEIALASGEEFELVFTIPPKYTEELSFDFTVIGTVHKGAGVFITTEEKRMEVPPIGWEHLDSR
>Mature_311_residues
MEREIIELFMKYLKKQGDLPLGDDAGALRIGDEWLVATNDMLVGKTDVPDIMTPEQVGFKAVTMNVSDIAAMGARPIGFL
FSLGVPRGFDGLYLEGVAKGIGNALEFYEIPVLSADTNEADDLIIDGIALGRTRHLLTRSGAKPGDLVCITGDIGRPLAG
LLVWKNDLDVSLSTKEILYEKLLEPRARVKEGIKIGNYANAAIDISDGLAKELHLVAEMSGVRIEVDASKLPIMEEVFEV
AELLRRDPLEIALASGEEFELVFTIPPKYTEELSFDFTVIGTVHKGAGVFITTEEKRMEVPPIGWEHLDSR

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=256, Percent_Identity=33.59375, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 34055; Mature: 34055

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEREIIELFMKYLKKQGDLPLGDDAGALRIGDEWLVATNDMLVGKTDVPDIMTPEQVGFK
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCEEECCCCCCCCCCCHHCCEE
AVTMNVSDIAAMGARPIGFLFSLGVPRGFDGLYLEGVAKGIGNALEFYEIPVLSADTNEA
EEEEEHHHHHHHCCCCHHHHEECCCCCCCCCHHHHHHHHHHCCHHHHEEEEEEECCCCCC
DDLIIDGIALGRTRHLLTRSGAKPGDLVCITGDIGRPLAGLLVWKNDLDVSLSTKEILYE
CCEEEECCHHCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEECCCCEEECHHHHHHH
KLLEPRARVKEGIKIGNYANAAIDISDGLAKELHLVAEMSGVRIEVDASKLPIMEEVFEV
HHHCHHHHHHCCCEECCCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
AELLRRDPLEIALASGEEFELVFTIPPKYTEELSFDFTVIGTVHKGAGVFITTEEKRMEV
HHHHHCCCCEEEEECCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCEEEEEECCCCCCC
PPIGWEHLDSR
CCCCHHHCCCC
>Mature Secondary Structure
MEREIIELFMKYLKKQGDLPLGDDAGALRIGDEWLVATNDMLVGKTDVPDIMTPEQVGFK
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCEEECCCCCCCCCCCHHCCEE
AVTMNVSDIAAMGARPIGFLFSLGVPRGFDGLYLEGVAKGIGNALEFYEIPVLSADTNEA
EEEEEHHHHHHHCCCCHHHHEECCCCCCCCCHHHHHHHHHHCCHHHHEEEEEEECCCCCC
DDLIIDGIALGRTRHLLTRSGAKPGDLVCITGDIGRPLAGLLVWKNDLDVSLSTKEILYE
CCEEEECCHHCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEECCCCEEECHHHHHHH
KLLEPRARVKEGIKIGNYANAAIDISDGLAKELHLVAEMSGVRIEVDASKLPIMEEVFEV
HHHCHHHHHHCCCEECCCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
AELLRRDPLEIALASGEEFELVFTIPPKYTEELSFDFTVIGTVHKGAGVFITTEEKRMEV
HHHHHCCCCEEEEECCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCEEEEEECCCCCCC
PPIGWEHLDSR
CCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]