Click here to switch to the map view.

The map label for this gene is phsB [H]

Identifier: 212223426

GI number: 212223426

Start: 247819

End: 248316

Strand: Reverse

Name: phsB [H]

Synonym: TON_0280

Alternate gene names: 212223426

Gene position: 248316-247819 (Counterclockwise)

Preceding gene: 212223427

Following gene: 212223425

Centisome position: 13.44

GC content: 53.21

Gene sequence:

>498_bases
ATGAGCAAAAAGATATTTATCGATTTTAAGCGCTGCATTGCCTGTAAGGCCTGTGAAGTCGCCTGTGAAATGGAGCACGG
GGAAGCGAGGATTAGGGTTTTTGAGTTCCCCGATCTGACCAGCGTCGCCTTCAACTGCCGCCACTGTGAAAAGGCTCCAT
GTATGGAAGTGTGTCCAGTTAACGCGCTCTCCAAGGACGATGATGGCGCAGTCGTTCTCGATCCCCTCAAGTGTATCGGC
TGTCTCATGTGCGGTCTGGCCTGTCCATTCGGCATTCCAAAGATAGACGAGTACAACAAGATAATGGACAAGTGCGACCT
CTGTGCCCACAGGAGAGCCGAAGGAAAGCTTCCTGCCTGTGTCTCAGCGTGCCCAACTGAGGCCCTCAAGTACGGCGACA
TAAACGATGTCCTCTGGGCCAGAGAAGGAAAGATAGTCGCCGAGCTTAAGGACATCGGCGACAGGACCAACGTCCTCGAG
GCCTACCTCATCAGATGA

Upstream 100 bases:

>100_bases
ACTTCAACTACCTGACCACGGACGTCCTCGACGAATACGCCAAGATGCCGGAGTTGAAGACGGCCGCGTGTAGGATCTCC
AAGGTTGAGGGGTGATTTAA

Downstream 100 bases:

>100_bases
AAGGAGGTGCCAGGATGAACGCCTCTCCCTTCATTATATCTTTTTTGATCCCCCTGCTCCTCGGTCCACTCCTGTTCAAA
TTAGACGGTAGAAAGGCCGA

Product: Oxidoreductase iron-sulfur protein

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSKKIFIDFKRCIACKACEVACEMEHGEARIRVFEFPDLTSVAFNCRHCEKAPCMEVCPVNALSKDDDGAVVLDPLKCIG
CLMCGLACPFGIPKIDEYNKIMDKCDLCAHRRAEGKLPACVSACPTEALKYGDINDVLWAREGKIVAELKDIGDRTNVLE
AYLIR

Sequences:

>Translated_165_residues
MSKKIFIDFKRCIACKACEVACEMEHGEARIRVFEFPDLTSVAFNCRHCEKAPCMEVCPVNALSKDDDGAVVLDPLKCIG
CLMCGLACPFGIPKIDEYNKIMDKCDLCAHRRAEGKLPACVSACPTEALKYGDINDVLWAREGKIVAELKDIGDRTNVLE
AYLIR
>Mature_164_residues
SKKIFIDFKRCIACKACEVACEMEHGEARIRVFEFPDLTSVAFNCRHCEKAPCMEVCPVNALSKDDDGAVVLDPLKCIGC
LMCGLACPFGIPKIDEYNKIMDKCDLCAHRRAEGKLPACVSACPTEALKYGDINDVLWAREGKIVAELKDIGDRTNVLEA
YLIR

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=166, Percent_Identity=37.9518072289157, Blast_Score=107, Evalue=3e-25,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=36.144578313253, Blast_Score=104, Evalue=3e-24,
Organism=Escherichia coli, GI2367245, Length=156, Percent_Identity=41.025641025641, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI2367345, Length=143, Percent_Identity=39.8601398601399, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI87082180, Length=161, Percent_Identity=37.888198757764, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1788811, Length=148, Percent_Identity=37.8378378378378, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI226510944, Length=138, Percent_Identity=38.4057971014493, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI87082179, Length=162, Percent_Identity=37.6543209876543, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1789067, Length=165, Percent_Identity=38.1818181818182, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789079, Length=204, Percent_Identity=34.3137254901961, Blast_Score=84, Evalue=3e-18,
Organism=Escherichia coli, GI1787749, Length=177, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=4e-18,
Organism=Escherichia coli, GI1790326, Length=177, Percent_Identity=32.7683615819209, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI1789370, Length=100, Percent_Identity=40, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787963, Length=149, Percent_Identity=30.2013422818792, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI87082114, Length=173, Percent_Identity=30.635838150289, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 18310; Mature: 18178

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
12.7 %Cys+Met (Translated Protein)
9.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIFIDFKRCIACKACEVACEMEHGEARIRVFEFPDLTSVAFNCRHCEKAPCMEVCPV
CCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCC
NALSKDDDGAVVLDPLKCIGCLMCGLACPFGIPKIDEYNKIMDKCDLCAHRRAEGKLPAC
CCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
VSACPTEALKYGDINDVLWAREGKIVAELKDIGDRTNVLEAYLIR
HHHCCHHHHHCCCCCHHEECCCCCHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
SKKIFIDFKRCIACKACEVACEMEHGEARIRVFEFPDLTSVAFNCRHCEKAPCMEVCPV
CCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCC
NALSKDDDGAVVLDPLKCIGCLMCGLACPFGIPKIDEYNKIMDKCDLCAHRRAEGKLPAC
CCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
VSACPTEALKYGDINDVLWAREGKIVAELKDIGDRTNVLEAYLIR
HHHCCHHHHHCCCCCHHEECCCCCHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]