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The map label for this gene is aspS [H]

Identifier: 212223203

GI number: 212223203

Start: 53132

End: 54034

Strand: Reverse

Name: aspS [H]

Synonym: TON_0058

Alternate gene names: 212223203

Gene position: 54034-53132 (Counterclockwise)

Preceding gene: 212223205

Following gene: 212223200

Centisome position: 2.92

GC content: 53.6

Gene sequence:

>903_bases
ATGAACGCTCTCCAAATTGTAACCCGGAAAATTGAACCAGTTATAGAAGTTCAAACGAGAGTGATTGACTATATGACAAG
ATACATGGTGAGCAAGGGTTTCAGGTGGATGCTCCCGGTGATGCTCAGCTCCATAACTGACCCCCTATGGCCAGACCCGG
CCGCCAGTGAGGCCCTAAAGCCGCCAGAAGTCGAGGTCTACGGCGAGAGGCTAAGGCTAACTCACAGCATGATACTCCAC
AAGCAGATGGCGATAGCTATGGGAATTAACAGGCTCTTTATACTCTCGCCGAACATAAGGCTCGAAGGGAGAAGCGCCGA
CGATGGCAGACACGCCTACGAGTTTACGCAGCTTGACTTCGAGATAGCCTATGCGAGCATGGACGACGTCATGAGCCTCA
TAGAGGAGCTGATAAGCGGATTGTTTAAGGAAGCTAGGAACTGGGGACTGGAGAGGGAAGTGCCCAAGGTAGCGCCACCC
TTCAAGCGCTTCACTCTTGAGGAAATTAAGGCCGAGTTTGGAAGCGAGGATGAGGCAAGTAAAGCCATGGACGAGCCCTT
CTGGGTGATAGACATTGAAAGGGAGTTCTACGACAGGGAAGACCCAGAGAGGCCGGGCCACTTCAGGAACTACGACCTCT
ATCTGCCAGAGGGATACGGCGAGGTTTCAAGCGGCGGCGAGAGGGAGTGGGAGTACGAAGTAATAGTCAGAAAAATTAAG
AAGGCAGGCATAAGCCTCGACGCCTTCAAACCCTACCTCGAAGTCGCCAAAGCGGGCCTCCTGAGGCCAAGCGCTGGAGC
GGGCATAGGCATCGAGAGGCTCATCAGGTACCTGGTCGGGGCTGAGCACATAGCGGAGGTTCAGCCCTTCCCGAGAATTC
CGGGGATTCCAGCAATCATATAA

Upstream 100 bases:

>100_bases
AAATTAACCTTTATTTTATGTCGAATTTTGCCCAAAATTCAACTTTTCTTGCACAAAGGACATAAAGGTGGAAATCACTG
ACAAAGAACAGGTGATGAAG

Downstream 100 bases:

>100_bases
TGACATGCTTTTCTTTCTTCTAATCAGTCAAAATTAACTGCACCGTGACTAGTAGAATGAAAGTAAGAGAATTCACGAAC
CACTTAGGTACTTGTGAATT

Product: asparagine synthetase A

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNALQIVTRKIEPVIEVQTRVIDYMTRYMVSKGFRWMLPVMLSSITDPLWPDPAASEALKPPEVEVYGERLRLTHSMILH
KQMAIAMGINRLFILSPNIRLEGRSADDGRHAYEFTQLDFEIAYASMDDVMSLIEELISGLFKEARNWGLEREVPKVAPP
FKRFTLEEIKAEFGSEDEASKAMDEPFWVIDIEREFYDREDPERPGHFRNYDLYLPEGYGEVSSGGEREWEYEVIVRKIK
KAGISLDAFKPYLEVAKAGLLRPSAGAGIGIERLIRYLVGAEHIAEVQPFPRIPGIPAII

Sequences:

>Translated_300_residues
MNALQIVTRKIEPVIEVQTRVIDYMTRYMVSKGFRWMLPVMLSSITDPLWPDPAASEALKPPEVEVYGERLRLTHSMILH
KQMAIAMGINRLFILSPNIRLEGRSADDGRHAYEFTQLDFEIAYASMDDVMSLIEELISGLFKEARNWGLEREVPKVAPP
FKRFTLEEIKAEFGSEDEASKAMDEPFWVIDIEREFYDREDPERPGHFRNYDLYLPEGYGEVSSGGEREWEYEVIVRKIK
KAGISLDAFKPYLEVAKAGLLRPSAGAGIGIERLIRYLVGAEHIAEVQPFPRIPGIPAII
>Mature_300_residues
MNALQIVTRKIEPVIEVQTRVIDYMTRYMVSKGFRWMLPVMLSSITDPLWPDPAASEALKPPEVEVYGERLRLTHSMILH
KQMAIAMGINRLFILSPNIRLEGRSADDGRHAYEFTQLDFEIAYASMDDVMSLIEELISGLFKEARNWGLEREVPKVAPP
FKRFTLEEIKAEFGSEDEASKAMDEPFWVIDIEREFYDREDPERPGHFRNYDLYLPEGYGEVSSGGEREWEYEVIVRKIK
KAGISLDAFKPYLEVAKAGLLRPSAGAGIGIERLIRYLVGAEHIAEVQPFPRIPGIPAII

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=321, Percent_Identity=25.5451713395639, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1787161, Length=340, Percent_Identity=23.2352941176471, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=267, Percent_Identity=27.3408239700375, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17136276, Length=301, Percent_Identity=25.9136212624585, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 34320; Mature: 34320

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALQIVTRKIEPVIEVQTRVIDYMTRYMVSKGFRWMLPVMLSSITDPLWPDPAASEALK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCC
PPEVEVYGERLRLTHSMILHKQMAIAMGINRLFILSPNIRLEGRSADDGRHAYEFTQLDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCHHHHHHHHHH
EIAYASMDDVMSLIEELISGLFKEARNWGLEREVPKVAPPFKRFTLEEIKAEFGSEDEAS
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
KAMDEPFWVIDIEREFYDREDPERPGHFRNYDLYLPEGYGEVSSGGEREWEYEVIVRKIK
HHHCCCEEEEEECHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
KAGISLDAFKPYLEVAKAGLLRPSAGAGIGIERLIRYLVGAEHIAEVQPFPRIPGIPAII
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNALQIVTRKIEPVIEVQTRVIDYMTRYMVSKGFRWMLPVMLSSITDPLWPDPAASEALK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCC
PPEVEVYGERLRLTHSMILHKQMAIAMGINRLFILSPNIRLEGRSADDGRHAYEFTQLDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCHHHHHHHHHH
EIAYASMDDVMSLIEELISGLFKEARNWGLEREVPKVAPPFKRFTLEEIKAEFGSEDEAS
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
KAMDEPFWVIDIEREFYDREDPERPGHFRNYDLYLPEGYGEVSSGGEREWEYEVIVRKIK
HHHCCCEEEEEECHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
KAGISLDAFKPYLEVAKAGLLRPSAGAGIGIERLIRYLVGAEHIAEVQPFPRIPGIPAII
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]