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The map label for this gene is vhuA [H]

Identifier: 212223197

GI number: 212223197

Start: 46791

End: 48038

Strand: Reverse

Name: vhuA [H]

Synonym: TON_0052

Alternate gene names: 212223197

Gene position: 48038-46791 (Counterclockwise)

Preceding gene: 212223198

Following gene: 212223196

Centisome position: 2.6

GC content: 54.17

Gene sequence:

>1248_bases
ATGATAATCGAGTTGAGGGAGTTCACGAGAGTTGAAGGCAACGGCAAAGCCGAGATAGTTGTCGAGAACGGTGAAGTTAA
GGACGTCAGGCTCAAAATCGTTGAAGGGCCGCGCTTCTTCGAGCTGCTGACTTTGGGAAGGCATTACTATGACGTTCCAG
ACTTGGAAGCGAGGATATGCGCCATATGCTACCTCTCACACAGCGTCGCCTCGGTTCTGGGCATAGAGAAGGCCTTCGGA
GTTGAAGTTTCGGAGGAGATCCAGCTCCTAAGAGAGCTCGGCCTCATCGGTGAATTACTGGAGAGCCACGCACTCCACCT
GTACCTGCTCGTTGCTCCAGACGTTTTCGGTTATCCTGACGCGATAAGAATGGCTACGAAGCACGGGGAGCTCGTCAAGG
AGGGGCTTGCACTGAAGGCCTTCGGCAACAGTATAAGGGAACTCATTGGAGGAAGGGAGATCCACGGCATAAACGTTAAA
CCCGGTGGATTTGGCAGGTATCCGACGGTTGAGGAACTTGAAAACATCGAGAGGGAGAGTGGGGCCCTCCTGAGACTCGC
AAGGAGAGCGGTGAGGCTATTCGCTTCGCTTGAGCCCTACGGCGAAAAGGCGGGACACTTCGTCGCGACGGACGGCTACC
TATGGGGCGACAAGCTGATTTCCGATGAGGATGGCTCTTTCCACTACACCGAGAGAATAGAGGAACGCTCACTGGTTTAC
AGCTTCGCAAAGCAGAGCCGCTATAAGGGTGAGCCCTTCTTCGTTGGCGCACTGCCGAGGCTCCTGCTCAAAGCAGAGAT
GCTGACACCCACAGCGAAGAGGCTCTTCGAGGAGCACAGGGAAAAGCTCGCCACCGGTTACGTCAGCTACAACAACCTCG
CTCAGGCCATAGAGCTCGTCTACGCGCTTGAAAGGGCGGGAGAGATAGCAAAGAAACTCCTCGACAAGGGCATAAAGGGT
GAAAACGTTCCCGTTGAAGTCAAAGAAGGCGAGGGCATAGGGTACGTGGAAGCGCCTAGGGGTGTCCTAATACATCACTA
CCGCATTGACTCTGGGGGCAAAATCGCCTACTCGAACATCATAACGCCCACGGCTTTAAACCACGCTATGATGGAGGCAA
GCCTGTTCAAGGAAGCGAGAAAACTCTACGGAGAGACGGACGAGACGGTACTCGTCCAGAGGCTGGAGGAAACGGTTAGA
GCCTTTGATCCGTGCATTTCCTGTTCAGTGCACATCGTGAAGCTTTAG

Upstream 100 bases:

>100_bases
GCGAGGTGAATCCTGCAAGTGAGTACGAGATACTCAAGGATCTGGGCTACGATGATGACTACATCCTCAGGAAGTTCAAG
ACCTTCGCGAGGTGGGAGCC

Downstream 100 bases:

>100_bases
CCGGCAATCTTTTTAATTTCCCCTTTTCAATCCCTCTCGGGGGAGAGCATGGGATTCCTAAGCAGACTGTTCGGTGGGAA
GAAGGAAACCGACACTGAGG

Product: H-II alpha (hydrogenase subunit alpha)

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MIIELREFTRVEGNGKAEIVVENGEVKDVRLKIVEGPRFFELLTLGRHYYDVPDLEARICAICYLSHSVASVLGIEKAFG
VEVSEEIQLLRELGLIGELLESHALHLYLLVAPDVFGYPDAIRMATKHGELVKEGLALKAFGNSIRELIGGREIHGINVK
PGGFGRYPTVEELENIERESGALLRLARRAVRLFASLEPYGEKAGHFVATDGYLWGDKLISDEDGSFHYTERIEERSLVY
SFAKQSRYKGEPFFVGALPRLLLKAEMLTPTAKRLFEEHREKLATGYVSYNNLAQAIELVYALERAGEIAKKLLDKGIKG
ENVPVEVKEGEGIGYVEAPRGVLIHHYRIDSGGKIAYSNIITPTALNHAMMEASLFKEARKLYGETDETVLVQRLEETVR
AFDPCISCSVHIVKL

Sequences:

>Translated_415_residues
MIIELREFTRVEGNGKAEIVVENGEVKDVRLKIVEGPRFFELLTLGRHYYDVPDLEARICAICYLSHSVASVLGIEKAFG
VEVSEEIQLLRELGLIGELLESHALHLYLLVAPDVFGYPDAIRMATKHGELVKEGLALKAFGNSIRELIGGREIHGINVK
PGGFGRYPTVEELENIERESGALLRLARRAVRLFASLEPYGEKAGHFVATDGYLWGDKLISDEDGSFHYTERIEERSLVY
SFAKQSRYKGEPFFVGALPRLLLKAEMLTPTAKRLFEEHREKLATGYVSYNNLAQAIELVYALERAGEIAKKLLDKGIKG
ENVPVEVKEGEGIGYVEAPRGVLIHHYRIDSGGKIAYSNIITPTALNHAMMEASLFKEARKLYGETDETVLVQRLEETVR
AFDPCISCSVHIVKL
>Mature_415_residues
MIIELREFTRVEGNGKAEIVVENGEVKDVRLKIVEGPRFFELLTLGRHYYDVPDLEARICAICYLSHSVASVLGIEKAFG
VEVSEEIQLLRELGLIGELLESHALHLYLLVAPDVFGYPDAIRMATKHGELVKEGLALKAFGNSIRELIGGREIHGINVK
PGGFGRYPTVEELENIERESGALLRLARRAVRLFASLEPYGEKAGHFVATDGYLWGDKLISDEDGSFHYTERIEERSLVY
SFAKQSRYKGEPFFVGALPRLLLKAEMLTPTAKRLFEEHREKLATGYVSYNNLAQAIELVYALERAGEIAKKLLDKGIKG
ENVPVEVKEGEGIGYVEAPRGVLIHHYRIDSGGKIAYSNIITPTALNHAMMEASLFKEARKLYGETDETVLVQRLEETVR
AFDPCISCSVHIVKL

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 46416; Mature: 46416

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIELREFTRVEGNGKAEIVVENGEVKDVRLKIVEGPRFFELLTLGRHYYDVPDLEARIC
CEEECCHHHCCCCCCCEEEEEECCCEEEEEEEEECCCHHHHHHHHCCHHCCCCCHHHHHH
AICYLSHSVASVLGIEKAFGVEVSEEIQLLRELGLIGELLESHALHLYLLVAPDVFGYPD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCH
AIRMATKHGELVKEGLALKAFGNSIRELIGGREIHGINVKPGGFGRYPTVEELENIERES
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
GALLRLARRAVRLFASLEPYGEKAGHFVATDGYLWGDKLISDEDGSFHYTERIEERSLVY
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCEECCCCCCCHHHHHHHHHHHHH
SFAKQSRYKGEPFFVGALPRLLLKAEMLTPTAKRLFEEHREKLATGYVSYNNLAQAIELV
HHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YALERAGEIAKKLLDKGIKGENVPVEVKEGEGIGYVEAPRGVLIHHYRIDSGGKIAYSNI
HHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECCCCEEEEEEEECCCCEEEECCC
ITPTALNHAMMEASLFKEARKLYGETDETVLVQRLEETVRAFDPCISCSVHIVKL
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHCCCEEEEEC
>Mature Secondary Structure
MIIELREFTRVEGNGKAEIVVENGEVKDVRLKIVEGPRFFELLTLGRHYYDVPDLEARIC
CEEECCHHHCCCCCCCEEEEEECCCEEEEEEEEECCCHHHHHHHHCCHHCCCCCHHHHHH
AICYLSHSVASVLGIEKAFGVEVSEEIQLLRELGLIGELLESHALHLYLLVAPDVFGYPD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCH
AIRMATKHGELVKEGLALKAFGNSIRELIGGREIHGINVKPGGFGRYPTVEELENIERES
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
GALLRLARRAVRLFASLEPYGEKAGHFVATDGYLWGDKLISDEDGSFHYTERIEERSLVY
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCEECCCCCCCHHHHHHHHHHHHH
SFAKQSRYKGEPFFVGALPRLLLKAEMLTPTAKRLFEEHREKLATGYVSYNNLAQAIELV
HHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YALERAGEIAKKLLDKGIKGENVPVEVKEGEGIGYVEAPRGVLIHHYRIDSGGKIAYSNI
HHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECCCCEEEEEEEECCCCEEEECCC
ITPTALNHAMMEASLFKEARKLYGETDETVLVQRLEETVRAFDPCISCSVHIVKL
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]