Click here to switch to the map view.

The map label for this gene is cls2 [H]

Identifier: 209554535

GI number: 209554535

Start: 858661

End: 860259

Strand: Reverse

Name: cls2 [H]

Synonym: UUR10_0692

Alternate gene names: 209554535

Gene position: 860259-858661 (Counterclockwise)

Preceding gene: 209554592

Following gene: 209554527

Centisome position: 98.37

GC content: 26.27

Gene sequence:

>1599_bases
ATGAGAAAAAGAATTCTAGCGAAATTTATCATAACATTCATTTGTGTGTTATTAATAATTAATGTTGCGCTAGTAGCGAC
ATATTTTCATCATCGACCAACAGCAATTGTTACAGATATTTTAATATCATTTTATATTTTAAATACCACAATCGGTTTAT
ATATTTTATCTTCAAAACACCGTCAAGACCGTTCTAAAAAATCGTGATTGTTTGTTTTCTTATTTTTACCAATTATTTCA
ATTATTTTATTTTGAATTTTTGGATCGCGCCCTTTTAAAGATAAAAGAATTAATGATATTATTGCTAATCGACGTGTGCT
TTTTAAATATGAAGATTATGAATTTTATCATAATTTTCAAAACATGAATTTTAGTAATAATGGCCTTGATTTAATTCGTA
AAATTGGTCGTTATAATTATCAACTTTTCCGTACGCCCATTTATTGTGATAATCAAATTACAATTATTAAAGAAGCAAAT
GAAATGTATGAACAAACCATTAAGTTAATAAGAAGCGCTAAAAAAACAATTTATTTACAATATTACATTATTGAAGATGG
TCGTTGACTACAATCGCTTGTTAAAGAGTTAATCGCAAAAAGTAATGAAGGCGTTAAAATATACATTTTATACGATCCAA
TTGGAACACTAAATAAATTCCCTAAAAAATATCGTGAACAACTAATTAATAATAATGTAGAAATTGCTTTTTTTAAAGCA
CGAGCTGATTATAAATTTAGAAGTACAATTAATTTTCGCTCTCATCGTAAATATTTAATTGTTGATGGACAAACTGCTAT
TAGTGGTGGTTCTAATATTGGTGATTCTTATTTAAATATTGATGCTAAAGTTGGTCATTGAGTTGATTTGAATTATATTT
TAACAGGTGGAATTGTGCACACAATTTTATTGGAGTTTATTCATGATTGATACTACTATACACCATTTTCAAAAACAAAT
AGTTTTAATGATGTTGTTAATAATATGCATCCCCAACCATATTTAGCATATGATGAAAACAATTTAGTAAATGATGATGT
TAAAAATAAAACCTTAACAACACTTTTACATACAGGACCTGATCAATCAACTAGTTCTTTAAATGAAATTTTAGTTATGG
CAATTGCTAATGCTAAAAAATCTATTACCATTTTTACACCATATTTATTCCCAACAGAAGACATTTTAATTGGTCTTAAA
GCTGCATCAAACGCTGGAATAAAAGTCAAAATTATTTTGCCTGGTCGTGTTGATTCATGAGGTTTTATTATTTCAATGAA
TCGTTTAAGTTACGAATCATTAATTAAATCTGGTGTTCAAATTTATGAATATAGTGGTTTTTTACACACTAAAGCAATTT
TAATTGATGATGATATATCATTGTTAGGTTCTTATAATCTTGACAATCGTGCGCTAGTAATTAATTATGAGTCCTTATTA
ACAGTTTATTCATTACAAGTTAACCAAGAAATCACTGAAGTTTTTAACCAATATTTACGTAATTCACAGTTAATTCATTT
AGAAGATTTAGATAATAAATTCACTTTTAGTGAACTAGTTCAAACAATATTTGTCAATATCTTTCAGCTACTTTTATAA

Upstream 100 bases:

>100_bases
TTTATATATGAACGAATTAATAATTATTGTATATTTCTAGTCTATTTTTAATTTTAAAACACATGTATAATTTTATTTGT
AATAAGGATAAAAATAAAAA

Downstream 100 bases:

>100_bases
TAAAAACCACTATTTAACAATATAAGGCACTAACATAAGGCTATAAATAATATAATTAAAATACATTTTATCAAATATGA
TATTTTATGAAAAAGGTTTA

Product: cardiolipin synthase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MRKRILAKFIITFICVLLIINVALVATYFHHRPTAIVTDILISFYILNTTIGLYILSSKHRQDRSKKSWLFVFLFLPIIS
IILFWIFGSRPFKDKRINDIIANRRVLFKYEDYEFYHNFQNMNFSNNGLDLIRKIGRYNYQLFRTPIYCDNQITIIKEAN
EMYEQTIKLIRSAKKTIYLQYYIIEDGRWLQSLVKELIAKSNEGVKIYILYDPIGTLNKFPKKYREQLINNNVEIAFFKA
RADYKFRSTINFRSHRKYLIVDGQTAISGGSNIGDSYLNIDAKVGHWVDLNYILTGGIVHTILLEFIHDWYYYTPFSKTN
SFNDVVNNMHPQPYLAYDENNLVNDDVKNKTLTTLLHTGPDQSTSSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLK
AASNAGIKVKIILPGRVDSWGFIISMNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLL
TVYSLQVNQEITEVFNQYLRNSQLIHLEDLDNKFTFSELVQTIFVNIFQLLL

Sequences:

>Translated_532_residues
MRKRILAKFIITFICVLLIINVALVATYFHHRPTAIVTDILISFYILNTTIGLYILSSKHRQDRSKKS*LFVFLFLPIIS
IILF*IFGSRPFKDKRINDIIANRRVLFKYEDYEFYHNFQNMNFSNNGLDLIRKIGRYNYQLFRTPIYCDNQITIIKEAN
EMYEQTIKLIRSAKKTIYLQYYIIEDGR*LQSLVKELIAKSNEGVKIYILYDPIGTLNKFPKKYREQLINNNVEIAFFKA
RADYKFRSTINFRSHRKYLIVDGQTAISGGSNIGDSYLNIDAKVGH*VDLNYILTGGIVHTILLEFIHD*YYYTPFSKTN
SFNDVVNNMHPQPYLAYDENNLVNDDVKNKTLTTLLHTGPDQSTSSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLK
AASNAGIKVKIILPGRVDS*GFIISMNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLL
TVYSLQVNQEITEVFNQYLRNSQLIHLEDLDNKFTFSELVQTIFVNIFQLLL
>Mature_532_residues
MRKRILAKFIITFICVLLIINVALVATYFHHRPTAIVTDILISFYILNTTIGLYILSSKHRQDRSKKS*LFVFLFLPIIS
IILF*IFGSRPFKDKRINDIIANRRVLFKYEDYEFYHNFQNMNFSNNGLDLIRKIGRYNYQLFRTPIYCDNQITIIKEAN
EMYEQTIKLIRSAKKTIYLQYYIIEDGR*LQSLVKELIAKSNEGVKIYILYDPIGTLNKFPKKYREQLINNNVEIAFFKA
RADYKFRSTINFRSHRKYLIVDGQTAISGGSNIGDSYLNIDAKVGH*VDLNYILTGGIVHTILLEFIHD*YYYTPFSKTN
SFNDVVNNMHPQPYLAYDENNLVNDDVKNKTLTTLLHTGPDQSTSSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLK
AASNAGIKVKIILPGRVDS*GFIISMNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLL
TVYSLQVNQEITEVFNQYLRNSQLIHLEDLDNKFTFSELVQTIFVNIFQLLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=498, Percent_Identity=23.2931726907631, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1787007, Length=350, Percent_Identity=25.4285714285714, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 60723; Mature: 60723

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRILAKFIITFICVLLIINVALVATYFHHRPTAIVTDILISFYILNTTIGLYILSSKH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECHH
RQDRSKKSLFVFLFLPIISIILFIFGSRPFKDKRINDIIANRRVLFKYEDYEFYHNFQNM
HCCCCHHHEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEEEECCHHHHHHCCC
NFSNNGLDLIRKIGRYNYQLFRTPIYCDNQITIIKEANEMYEQTIKLIRSAKKTIYLQYY
CCCCCCHHHHHHHHCCCEEEEECCEEECCEEEEEECHHHHHHHHHHHHHHCCCEEEEEEE
IIEDGRLQSLVKELIAKSNEGVKIYILYDPIGTLNKFPKKYREQLINNNVEIAFFKARAD
EECCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCC
YKFRSTINFRSHRKYLIVDGQTAISGGSNIGDSYLNIDAKVGHVDLNYILTGGIVHTILL
CEEEEEECCCCCCEEEEEECCEEECCCCCCCCCEEEEEEEECEEEEEEEEEHHHHHHHHH
EFIHDYYYTPFSKTNSFNDVVNNMHPQPYLAYDENNLVNDDVKNKTLTTLLHTGPDQSTS
HHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEHEEECCCCCCHH
SLNEILVMAIANAKKSITIFTPYLFPTEDILIGLKAASNAGIKVKIILPGRVDSGFIISM
HHHHHHHHEEECCCCEEEEEECCCCCCCHHEEEEEECCCCCEEEEEEECCCCCCCEEEEE
NRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLLTVYSLQ
CCCCHHHHHHCCCEEEEECCEEEEEEEEEECCHHHHCCCCCCCCEEEEEHHHHEEEHEEH
VNQEITEVFNQYLRNSQLIHLEDLDNKFTFSELVQTIFVNIFQLLL
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MRKRILAKFIITFICVLLIINVALVATYFHHRPTAIVTDILISFYILNTTIGLYILSSKH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECHH
RQDRSKKSLFVFLFLPIISIILFIFGSRPFKDKRINDIIANRRVLFKYEDYEFYHNFQNM
HCCCCHHHEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEEEECCHHHHHHCCC
NFSNNGLDLIRKIGRYNYQLFRTPIYCDNQITIIKEANEMYEQTIKLIRSAKKTIYLQYY
CCCCCCHHHHHHHHCCCEEEEECCEEECCEEEEEECHHHHHHHHHHHHHHCCCEEEEEEE
IIEDGRLQSLVKELIAKSNEGVKIYILYDPIGTLNKFPKKYREQLINNNVEIAFFKARAD
EECCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCC
YKFRSTINFRSHRKYLIVDGQTAISGGSNIGDSYLNIDAKVGHVDLNYILTGGIVHTILL
CEEEEEECCCCCCEEEEEECCEEECCCCCCCCCEEEEEEEECEEEEEEEEEHHHHHHHHH
EFIHDYYYTPFSKTNSFNDVVNNMHPQPYLAYDENNLVNDDVKNKTLTTLLHTGPDQSTS
HHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEHEEECCCCCCHH
SLNEILVMAIANAKKSITIFTPYLFPTEDILIGLKAASNAGIKVKIILPGRVDSGFIISM
HHHHHHHHEEECCCCEEEEEECCCCCCCHHEEEEEECCCCCEEEEEEECCCCCCCEEEEE
NRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLLTVYSLQ
CCCCHHHHHHCCCEEEEECCEEEEEEEEEECCHHHHCCCCCCCCEEEEEHHHHEEEHEEH
VNQEITEVFNQYLRNSQLIHLEDLDNKFTFSELVQTIFVNIFQLLL
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]