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The map label for this gene is ruvB

Identifier: 209554525

GI number: 209554525

Start: 532749

End: 533687

Strand: Reverse

Name: ruvB

Synonym: UUR10_0482

Alternate gene names: 209554525

Gene position: 533687-532749 (Counterclockwise)

Preceding gene: 209554366

Following gene: 209554420

Centisome position: 61.03

GC content: 25.45

Gene sequence:

>939_bases
ATGAAAACTAATAATGAATTTCGTCCTCAGTATCTAAAAGATTTTATTGGTAAAGACCAATTAAAAAGTAATTTAAAGAT
TTATTTAAATGCTACTAAACGCTTAAAAAGTAGTTTTGATCATACACTATTACATGGATTAGCTGGAACTGGAAAAACAA
CTCTAGCAACAATTATTGCTAATGAAATGGGCGTTGATTGTCATATGACACAAGGGAATTTATTAAATAAACCTGTGGAT
ATTATTAATTTACTATCTTTAATTAAAGAAAATGATGTCATTTTTGTTGATGAAATTCACGCATGTGGTTTAGCAGCATT
TGAAACACTTTATTCTGTATTAGAAGATTTTTGTATCGATATTAGTATTGGTAAGGATTTTAATGCTAAAATGACAAGAT
TAAAAGTTCCACATTTTACATTAATTGGTGCAACAACAATGCTTGGAAAAATTCCTGAACCTTTAGAAGAACGTTTTGGT
CATGTTTTTTATTTAAGCGAATATGAAACATCTGAGATTGCAGCAATCATTTTAAAAAATAATCAAATCCACTTTCAGAT
TAATTTAAATGATCAAGAAATCGATTTAATTGCCAATAGTGCTAAAGGTATTCCGCGATTAGCTAACCGCTTATTAAAAC
GAGTTGTTGATTTTAAAATTAATGGATTTGATAATATTAAAAATATTTTTGAAAAAATTCAAATTTATGATTTTGGATTA
GAAGAACAAGATATTAATTATTTAAATGTTTTATACCAACAAGAAAATGAAATTGGTTTAAAATCAATTGCACAAATTTT
ACGTTTAGATCAATATACAATTGAAACCAAAATTGAACCTTATTTAATTCAACACCATTTTATTAACAAAAATTTAAGAG
GTCGTAAAATTACAGCTAAAGGAATTGAGTTTTTAAAAAATAATCAACTAATCAAATAA

Upstream 100 bases:

>100_bases
GATATTGAATATGCAATCACTAAAGTTAAAGTTGATACACAGCAAAACATTGATATTAGTGAAATTATAGGATGTGCTAT
TAAAGAAATTTCTTTACGCC

Downstream 100 bases:

>100_bases
AAATAATTGTTTTTTAACACTTTCTTTTGGTACTAATTTATTTAATGAAACGCCAATCAAACGTACTAATTCATCTTTAT
AAACTTTTTTAAATAAATTT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKTNNEFRPQYLKDFIGKDQLKSNLKIYLNATKRLKSSFDHTLLHGLAGTGKTTLATIIANEMGVDCHMTQGNLLNKPVD
IINLLSLIKENDVIFVDEIHACGLAAFETLYSVLEDFCIDISIGKDFNAKMTRLKVPHFTLIGATTMLGKIPEPLEERFG
HVFYLSEYETSEIAAIILKNNQIHFQINLNDQEIDLIANSAKGIPRLANRLLKRVVDFKINGFDNIKNIFEKIQIYDFGL
EEQDINYLNVLYQQENEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITAKGIEFLKNNQLIK

Sequences:

>Translated_312_residues
MKTNNEFRPQYLKDFIGKDQLKSNLKIYLNATKRLKSSFDHTLLHGLAGTGKTTLATIIANEMGVDCHMTQGNLLNKPVD
IINLLSLIKENDVIFVDEIHACGLAAFETLYSVLEDFCIDISIGKDFNAKMTRLKVPHFTLIGATTMLGKIPEPLEERFG
HVFYLSEYETSEIAAIILKNNQIHFQINLNDQEIDLIANSAKGIPRLANRLLKRVVDFKINGFDNIKNIFEKIQIYDFGL
EEQDINYLNVLYQQENEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITAKGIEFLKNNQLIK
>Mature_312_residues
MKTNNEFRPQYLKDFIGKDQLKSNLKIYLNATKRLKSSFDHTLLHGLAGTGKTTLATIIANEMGVDCHMTQGNLLNKPVD
IINLLSLIKENDVIFVDEIHACGLAAFETLYSVLEDFCIDISIGKDFNAKMTRLKVPHFTLIGATTMLGKIPEPLEERFG
HVFYLSEYETSEIAAIILKNNQIHFQINLNDQEIDLIANSAKGIPRLANRLLKRVVDFKINGFDNIKNIFEKIQIYDFGL
EEQDINYLNVLYQQENEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITAKGIEFLKNNQLIK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=307, Percent_Identity=34.8534201954397, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_UREU1 (B5ZBT5)

Other databases:

- EMBL:   CP001184
- RefSeq:   YP_002284875.1
- GeneID:   6983813
- GenomeReviews:   CP001184_GR
- KEGG:   uue:UUR10_0482
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   UURE95664:UUR10_0482-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 35771; Mature: 35771

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNNEFRPQYLKDFIGKDQLKSNLKIYLNATKRLKSSFDHTLLHGLAGTGKTTLATIIA
CCCCCCCCHHHHHHHHCHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NEMGVDCHMTQGNLLNKPVDIINLLSLIKENDVIFVDEIHACGLAAFETLYSVLEDFCID
HHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHEEE
ISIGKDFNAKMTRLKVPHFTLIGATTMLGKIPEPLEERFGHVFYLSEYETSEIAAIILKN
EEECCCCCCCEEEEECCCEEEHHHHHHHCCCCCHHHHHCCEEEEEECCCCCCEEEEEEEC
NQIHFQINLNDQEIDLIANSAKGIPRLANRLLKRVVDFKINGFDNIKNIFEKIQIYDFGL
CEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCC
EEQDINYLNVLYQQENEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITAK
CCCCCHHHHHEECCCCCCCHHHHHHHHHHHHHEEECCCCCEEEEHHHHCCCCCCCEEEHH
GIEFLKNNQLIK
HHHHHHCCCCCC
>Mature Secondary Structure
MKTNNEFRPQYLKDFIGKDQLKSNLKIYLNATKRLKSSFDHTLLHGLAGTGKTTLATIIA
CCCCCCCCHHHHHHHHCHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NEMGVDCHMTQGNLLNKPVDIINLLSLIKENDVIFVDEIHACGLAAFETLYSVLEDFCID
HHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHEEE
ISIGKDFNAKMTRLKVPHFTLIGATTMLGKIPEPLEERFGHVFYLSEYETSEIAAIILKN
EEECCCCCCCEEEEECCCEEEHHHHHHHCCCCCHHHHHCCEEEEEECCCCCCEEEEEEEC
NQIHFQINLNDQEIDLIANSAKGIPRLANRLLKRVVDFKINGFDNIKNIFEKIQIYDFGL
CEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCC
EEQDINYLNVLYQQENEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITAK
CCCCCHHHHHEECCCCCCCHHHHHHHHHHHHHEEECCCCCEEEEHHHHCCCCCCCEEEHH
GIEFLKNNQLIK
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA