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The map label for this gene is topA [H]

Identifier: 209554326

GI number: 209554326

Start: 860923

End: 862959

Strand: Reverse

Name: topA [H]

Synonym: UUR10_0695

Alternate gene names: 209554326

Gene position: 862959-860923 (Counterclockwise)

Preceding gene: 209554435

Following gene: 209554592

Centisome position: 98.68

GC content: 28.03

Gene sequence:

>2037_bases
ATGCAAAAAGATTTGATAGTAGTAGAGTCACCAAATAAGATTAAATCGATTCAATCGTATTTAGGTGATGGATATAATGT
AATAGCAACAGGTGGCCATTTACGCGAACTTGCTAAAAAATCAGGATACGATCCAAAAACTTATGATTTAAAATGAGAAA
TAATCAAAACAACAAATAAAAAAATATCAAAAAAACAACAAATAAAAGCAATCATAGATTTAGCAAAAAAATCAGATAAT
ATTTATTTAGCAACTGACCCTGATCGTGAAGGCGAAGCTATTAGTTGACATGTTTATGATTTATTACCAAAAAAAGAGAA
AGAACGTGTTAGAAGAATTACTTTTAATGAAATCACACAAAAAGCAATTTTAAAATCAATTGACGAAAAACATGATATTG
ATTTTAATTTGGTGGACTCACAATTTGCAAGACGAATTTTAGATCGAGTTATTGGTTATAAACTTTCTGGATTAGTTAAA
AAAAATCTGCATGGTCTATCAGCTGGACGAGTGCAATCAATTGCTTTATTATTTGTAGTTGAAAGAGAATTAGAACGACG
AGCTTTTGTTAAAGAAGAATGATACCAAGTTGAAGGAACGATTGAAAAAAATATTTTTGTATCTTTAAGAAAAGTTCCAT
ACGACGTTGAATTATATAATCAAAAAGAGAATGGCGATAGTGATTTAAAATTTTTACATCGTAAAGATGCTCAACGTGTT
ATTGATGAATTAGGAGATATTTTTAAAGTTTATAATGTTGATGATTTAAAAATCACTAAAGGGGATGTTCAATTACCATT
AACAACTGATAAATTACTACAAATGGCGTCAAATAATTTCGGTTGAAGTGCTAATAAAACAACACTAGTAGCCCAACAAA
TGTTTGAGGGTTTAGAAATTAATAATCAACAATTAGCTTTAATAACTTATCCAAGAACAGATAGCAAACGATTAAGCGAT
GATTTTTTATTAGAAGCTAATAAATTTATTGAAGATAATTATGGTAATGATTATTTAGAAAATTTTAAGAATAAAAAAAT
CAATAAAAAAGAAGAAAATGTACAAGATGCACACGAGGCAATTCGTCCTGTTGACATTAACTTAAAACCTGAAGAAATTA
AAGAGCATGTTTCAGCAGATATTTACAAATTATATAATTTAGTTTGAAACAAAACTTTAGCTGCATTAATGAAAGTTCCA
ACATTTAATAAGCAAGTTATTCGTTTCATTAGTAATGAATATAAATTTTATACATCATATAAAACAATTAGAACTCTAGG
ATATTGAGTTTTAGATTTTTATAAAAAACAACGTGATGAATTAAAGTTTCAACCACCAGTTATTAATGTTGGTGATGAAT
TTAAAAAAGAAAACATTGAATTGGTTCAAAAAGAAACAACTCCACCACCTTATTACACAGAAGCATCTTTAATTGCTGCA
TTAAAAAATGCTGGTGTTGGTCGTCCATCAACATATGCGACAATGGCTACGATTGGAACAACTCGAGGTTATATTAATAA
AGAAAAAGGAAAACTAATTCCAACAGAGTTAGGGATTAAAGTTATTCAAGAACTAAAAGAAGCCTTCCCACGAGTTATTT
CTATTAAATTTACATCTAAAATGGAAGATCGTTTAGATGTTGTTGCTAATGGTGAATTATTTTGAAAAGATTTAGTTAGT
TCTTTCATACCTGTTTTTGAAGAAGAAGTAAAACAAGCTTATACTAAAATTGAAAAAGTTCCAGATGAAAAAATTAATCG
ATTATGTCCAATGTGTGAACATGATTTAGTGATTAAAAGATCTAGAACAAATGCTCAATTTATTGCCTGTTCAAACTTTC
CTACCTGTCGTTTTACAGAATCATTAGAAAAACCAGAAATTCTTGATGAATTATGTCCAACTTGTTCAGAACCATTAATT
AAACGTAAGAATCGTAAGAATAAATACTTTATTGGATGTACAGGTTATCCACAATGTAACTACATTCGTAATGTTAATGA
ATCAAATAATGATAAAAACAATTCTGATCAAGAATAA

Upstream 100 bases:

>100_bases
TATTAAAAACACAAAATTAATAAATTAAACAAGAAACATAATATTATAATTATTTACAATGATTAAAACGAGCAAATGAT
TATAATAAATGGGAGAAAAA

Downstream 100 bases:

>100_bases
TATATAAAATACAAAAAGGAAAATCTTATGTTAATTAAATTAGAAAACAACCAAAACTTAAATCAAATACTAAAAGATAA
TCATTCAAAACCAGTTTTAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MQKDLIVVESPNKIKSIQSYLGDGYNVIATGGHLRELAKKSGYDPKTYDLKWEIIKTTNKKISKKQQIKAIIDLAKKSDN
IYLATDPDREGEAISWHVYDLLPKKEKERVRRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVK
KNLHGLSAGRVQSIALLFVVERELERRAFVKEEWYQVEGTIEKNIFVSLRKVPYDVELYNQKENGDSDLKFLHRKDAQRV
IDELGDIFKVYNVDDLKITKGDVQLPLTTDKLLQMASNNFGWSANKTTLVAQQMFEGLEINNQQLALITYPRTDSKRLSD
DFLLEANKFIEDNYGNDYLENFKNKKINKKEENVQDAHEAIRPVDINLKPEEIKEHVSADIYKLYNLVWNKTLAALMKVP
TFNKQVIRFISNEYKFYTSYKTIRTLGYWVLDFYKKQRDELKFQPPVINVGDEFKKENIELVQKETTPPPYYTEASLIAA
LKNAGVGRPSTYATMATIGTTRGYINKEKGKLIPTELGIKVIQELKEAFPRVISIKFTSKMEDRLDVVANGELFWKDLVS
SFIPVFEEEVKQAYTKIEKVPDEKINRLCPMCEHDLVIKRSRTNAQFIACSNFPTCRFTESLEKPEILDELCPTCSEPLI
KRKNRKNKYFIGCTGYPQCNYIRNVNESNNDKNNSDQE

Sequences:

>Translated_678_residues
MQKDLIVVESPNKIKSIQSYLGDGYNVIATGGHLRELAKKSGYDPKTYDLK*EIIKTTNKKISKKQQIKAIIDLAKKSDN
IYLATDPDREGEAIS*HVYDLLPKKEKERVRRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVK
KNLHGLSAGRVQSIALLFVVERELERRAFVKEE*YQVEGTIEKNIFVSLRKVPYDVELYNQKENGDSDLKFLHRKDAQRV
IDELGDIFKVYNVDDLKITKGDVQLPLTTDKLLQMASNNFG*SANKTTLVAQQMFEGLEINNQQLALITYPRTDSKRLSD
DFLLEANKFIEDNYGNDYLENFKNKKINKKEENVQDAHEAIRPVDINLKPEEIKEHVSADIYKLYNLV*NKTLAALMKVP
TFNKQVIRFISNEYKFYTSYKTIRTLGY*VLDFYKKQRDELKFQPPVINVGDEFKKENIELVQKETTPPPYYTEASLIAA
LKNAGVGRPSTYATMATIGTTRGYINKEKGKLIPTELGIKVIQELKEAFPRVISIKFTSKMEDRLDVVANGELF*KDLVS
SFIPVFEEEVKQAYTKIEKVPDEKINRLCPMCEHDLVIKRSRTNAQFIACSNFPTCRFTESLEKPEILDELCPTCSEPLI
KRKNRKNKYFIGCTGYPQCNYIRNVNESNNDKNNSDQE
>Mature_678_residues
MQKDLIVVESPNKIKSIQSYLGDGYNVIATGGHLRELAKKSGYDPKTYDLK*EIIKTTNKKISKKQQIKAIIDLAKKSDN
IYLATDPDREGEAIS*HVYDLLPKKEKERVRRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVK
KNLHGLSAGRVQSIALLFVVERELERRAFVKEE*YQVEGTIEKNIFVSLRKVPYDVELYNQKENGDSDLKFLHRKDAQRV
IDELGDIFKVYNVDDLKITKGDVQLPLTTDKLLQMASNNFG*SANKTTLVAQQMFEGLEINNQQLALITYPRTDSKRLSD
DFLLEANKFIEDNYGNDYLENFKNKKINKKEENVQDAHEAIRPVDINLKPEEIKEHVSADIYKLYNLV*NKTLAALMKVP
TFNKQVIRFISNEYKFYTSYKTIRTLGY*VLDFYKKQRDELKFQPPVINVGDEFKKENIELVQKETTPPPYYTEASLIAA
LKNAGVGRPSTYATMATIGTTRGYINKEKGKLIPTELGIKVIQELKEAFPRVISIKFTSKMEDRLDVVANGELF*KDLVS
SFIPVFEEEVKQAYTKIEKVPDEKINRLCPMCEHDLVIKRSRTNAQFIACSNFPTCRFTESLEKPEILDELCPTCSEPLI
KRKNRKNKYFIGCTGYPQCNYIRNVNESNNDKNNSDQE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=520, Percent_Identity=25.3846153846154, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI10835218, Length=609, Percent_Identity=22.3316912972085, Blast_Score=89, Evalue=2e-17,
Organism=Escherichia coli, GI1787529, Length=651, Percent_Identity=32.8725038402458, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1788061, Length=632, Percent_Identity=24.8417721518987, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI48994931, Length=79, Percent_Identity=36.7088607594937, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32563869, Length=584, Percent_Identity=24.1438356164384, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=473, Percent_Identity=25.369978858351, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24585251, Length=693, Percent_Identity=24.6753246753247, Blast_Score=108, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77129; Mature: 77129

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKDLIVVESPNKIKSIQSYLGDGYNVIATGGHLRELAKKSGYDPKTYDLKEIIKTTNKK
CCCCEEEEECCHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ISKKQQIKAIIDLAKKSDNIYLATDPDREGEAISHVYDLLPKKEKERVRRITFNEITQKA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVKKNLHGLSAGRVQSIALLFVVER
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ELERRAFVKEEYQVEGTIEKNIFVSLRKVPYDVELYNQKENGDSDLKFLHRKDAQRVIDE
HHHHHHHHHHHHHCCCEECCCCEEEHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LGDIFKVYNVDDLKITKGDVQLPLTTDKLLQMASNNFGSANKTTLVAQQMFEGLEINNQQ
HHHHHHHCCCCCEEEECCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCE
LALITYPRTDSKRLSDDFLLEANKFIEDNYGNDYLENFKNKKINKKEENVQDAHEAIRPV
EEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCC
DINLKPEEIKEHVSADIYKLYNLVNKTLAALMKVPTFNKQVIRFISNEYKFYTSYKTIRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHH
LGYVLDFYKKQRDELKFQPPVINVGDEFKKENIELVQKETTPPPYYTEASLIAALKNAGV
HHHHHHHHHHHHHCCCCCCCEECCCHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHCCC
GRPSTYATMATIGTTRGYINKEKGKLIPTELGIKVIQELKEAFPRVISIKFTSKMEDRLD
CCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
VVANGELFKDLVSSFIPVFEEEVKQAYTKIEKVPDEKINRLCPMCEHDLVIKRSRTNAQF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCEE
IACSNFPTCRFTESLEKPEILDELCPTCSEPLIKRKNRKNKYFIGCTGYPQCNYIRNVNE
EEECCCCCCCCHHCCCCCHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHCCCC
SNNDKNNSDQE
CCCCCCCCCCC
>Mature Secondary Structure
MQKDLIVVESPNKIKSIQSYLGDGYNVIATGGHLRELAKKSGYDPKTYDLKEIIKTTNKK
CCCCEEEEECCHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ISKKQQIKAIIDLAKKSDNIYLATDPDREGEAISHVYDLLPKKEKERVRRITFNEITQKA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVKKNLHGLSAGRVQSIALLFVVER
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ELERRAFVKEEYQVEGTIEKNIFVSLRKVPYDVELYNQKENGDSDLKFLHRKDAQRVIDE
HHHHHHHHHHHHHCCCEECCCCEEEHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LGDIFKVYNVDDLKITKGDVQLPLTTDKLLQMASNNFGSANKTTLVAQQMFEGLEINNQQ
HHHHHHHCCCCCEEEECCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCE
LALITYPRTDSKRLSDDFLLEANKFIEDNYGNDYLENFKNKKINKKEENVQDAHEAIRPV
EEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCC
DINLKPEEIKEHVSADIYKLYNLVNKTLAALMKVPTFNKQVIRFISNEYKFYTSYKTIRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHH
LGYVLDFYKKQRDELKFQPPVINVGDEFKKENIELVQKETTPPPYYTEASLIAALKNAGV
HHHHHHHHHHHHHCCCCCCCEECCCHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHCCC
GRPSTYATMATIGTTRGYINKEKGKLIPTELGIKVIQELKEAFPRVISIKFTSKMEDRLD
CCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
VVANGELFKDLVSSFIPVFEEEVKQAYTKIEKVPDEKINRLCPMCEHDLVIKRSRTNAQF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCEE
IACSNFPTCRFTESLEKPEILDELCPTCSEPLIKRKNRKNKYFIGCTGYPQCNYIRNVNE
EEECCCCCCCCHHCCCCCHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHCCCC
SNNDKNNSDQE
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]