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The map label for this gene is nadE [H]

Identifier: 209554252

GI number: 209554252

Start: 556104

End: 556871

Strand: Reverse

Name: nadE [H]

Synonym: UUR10_0504

Alternate gene names: 209554252

Gene position: 556871-556104 (Counterclockwise)

Preceding gene: 209554030

Following gene: 209554194

Centisome position: 63.68

GC content: 23.57

Gene sequence:

>768_bases
ATGAATAATGATTTAGTTAAATATTTACAATGATTAAAAAATGATTTTATTAAGTTTTTAAATGATGCTCAAACTAATAA
TATTATTTTAGGAATTTCAGGTGGTGTTGATAGTGCATTAACACTAGCTATTTTAAATGATTTAAAAAAAGAGTATGCTT
TAAATATCTATGCTTATTTTTTAGATATTCACAATTCAAATTTAGATTATGAATGCGTTAGTGAACTAAAAAAAGTTTAT
CCTAATATTGAAATAATTAATCTTGTTGATATTTATAATAGTTATCGTTTGTTGATTAATAAAAAAACAAATGATAATTA
TGTTCTTTATAATTTAAAACCAAAAATTCGCACTAACTACTTATATGCAATGGCAAATGCGTATAGAGGTGTTGTTGTAA
GTAATTTAAATTATGATGAATATATTTTAGGTTTTTTTACAAAACATGGTGATAGCGCTGCTGATTATCATATGCTAATT
GGTTTATTAAAAAAACACATTTATGAATTAGGTGCTTACTATAGCTTACCAACTAAAATTTTAAATCGTGCTCCAACACC
TTCAAACGAAGATGATGAACATCAAACAGATGAGAGTTTTTTTGGTTTTACATACGCTGATCTTGATCAATTTTTACTAT
ATCGTAAAATTGATCCTACAATTGCTGATATGATTAAAAAACGCTATGAAATTAATGCTCATAAACATTTTGTTTTTGAT
AAGAAAAAATTATTTTTGAATTATAAATTAGGAACTAAACATGAATAA

Upstream 100 bases:

>100_bases
AGTATTAATTTTAACCAACAATTATTACAAGCTGGCGCAATAGCTGGTGATAGTATTAAAATATATGATATTACATTAGA
ATTTGAAGAGTAAGTAAACT

Downstream 100 bases:

>100_bases
TAAAAAAAGCAAAAAATTTTATGATTTTTTAAAAACAATCAAGGGTTTTAAAGTAATTACAATTATTTCATACATCTTTG
TTGCTATCTTATTAGCAATA

Product: NAD+ synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNNDLVKYLQWLKNDFIKFLNDAQTNNIILGISGGVDSALTLAILNDLKKEYALNIYAYFLDIHNSNLDYECVSELKKVY
PNIEIINLVDIYNSYRLLINKKTNDNYVLYNLKPKIRTNYLYAMANAYRGVVVSNLNYDEYILGFFTKHGDSAADYHMLI
GLLKKHIYELGAYYSLPTKILNRAPTPSNEDDEHQTDESFFGFTYADLDQFLLYRKIDPTIADMIKKRYEINAHKHFVFD
KKKLFLNYKLGTKHE

Sequences:

>Translated_255_residues
MNNDLVKYLQ*LKNDFIKFLNDAQTNNIILGISGGVDSALTLAILNDLKKEYALNIYAYFLDIHNSNLDYECVSELKKVY
PNIEIINLVDIYNSYRLLINKKTNDNYVLYNLKPKIRTNYLYAMANAYRGVVVSNLNYDEYILGFFTKHGDSAADYHMLI
GLLKKHIYELGAYYSLPTKILNRAPTPSNEDDEHQTDESFFGFTYADLDQFLLYRKIDPTIADMIKKRYEINAHKHFVFD
KKKLFLNYKLGTKHE
>Mature_255_residues
MNNDLVKYLQ*LKNDFIKFLNDAQTNNIILGISGGVDSALTLAILNDLKKEYALNIYAYFLDIHNSNLDYECVSELKKVY
PNIEIINLVDIYNSYRLLINKKTNDNYVLYNLKPKIRTNYLYAMANAYRGVVVSNLNYDEYILGFFTKHGDSAADYHMLI
GLLKKHIYELGAYYSLPTKILNRAPTPSNEDDEHQTDESFFGFTYADLDQFLLYRKIDPTIADMIKKRYEINAHKHFVFD
KKKLFLNYKLGTKHE

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=229, Percent_Identity=29.2576419213974, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 29649; Mature: 29649

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDLVKYLQLKNDFIKFLNDAQTNNIILGISGGVDSALTLAILNDLKKEYALNIYAYFL
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEE
DIHNSNLDYECVSELKKVYPNIEIINLVDIYNSYRLLINKKTNDNYVLYNLKPKIRTNYL
EEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCEEEEECCCCHHHHHH
YAMANAYRGVVVSNLNYDEYILGFFTKHGDSAADYHMLIGLLKKHIYELGAYYSLPTKIL
HHHHHHHCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHCCHHHHH
NRAPTPSNEDDEHQTDESFFGFTYADLDQFLLYRKIDPTIADMIKKRYEINAHKHFVFDK
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEC
KKLFLNYKLGTKHE
EEEEEEEECCCCCC
>Mature Secondary Structure
MNNDLVKYLQLKNDFIKFLNDAQTNNIILGISGGVDSALTLAILNDLKKEYALNIYAYFL
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEE
DIHNSNLDYECVSELKKVYPNIEIINLVDIYNSYRLLINKKTNDNYVLYNLKPKIRTNYL
EEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCEEEEECCCCHHHHHH
YAMANAYRGVVVSNLNYDEYILGFFTKHGDSAADYHMLIGLLKKHIYELGAYYSLPTKIL
HHHHHHHCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHCCHHHHH
NRAPTPSNEDDEHQTDESFFGFTYADLDQFLLYRKIDPTIADMIKKRYEINAHKHFVFDK
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEC
KKLFLNYKLGTKHE
EEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]