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The map label for this gene is tilS [H]

Identifier: 209554128

GI number: 209554128

Start: 101034

End: 102014

Strand: Reverse

Name: tilS [H]

Synonym: UUR10_0083

Alternate gene names: 209554128

Gene position: 102014-101034 (Counterclockwise)

Preceding gene: 209554113

Following gene: 209554040

Centisome position: 11.67

GC content: 24.06

Gene sequence:

>981_bases
TTGACAAAGTTATGAACAAATTTAATTAATAAAATTACTAATAAAAAATATTTAGCTGCTGTTTCAGGCGGACCTGATTC
TATGGCTATGCTAAATATGTATAAAAGAAATATTAGTGTTGTTTGTCATGTAAATTATCATAAACGTGAATCTGCTGATC
GAGATCAAGAAATTGTAGTTGATTTTTGTAAAAAAAATAATTTACCAATTGAAATTTTAGATGTTGATGAAAAAGTTTAT
GAGAAATATGCTCATATTGATAATTTTCAAGCTAAAGCTCGTTTAATTCGTTATGATTTCTTTAAAGAAATTGGTAAAAA
ATACAACATACAACACTTATATATAGCTCACAATTTTGATGATTTTTTAGAAACAGCGTATATGCAACGCGCTCGTCAAT
CAAAAGCTTTATTTTATGGTATTAAAGAAAGTAATGTTGTTAATGGAATGATTGTTAAACGTCCAGTATTATTTATACGT
AAACAAACATTACAACGCTATTGTGATGAAAATAAAATTAAATATGGAATTGATGAAACTAATGAATTAGACATATACGA
ACGTAATCGTGTACGCAAAACCATTAGTAATTGAAGTTTAAACGAAGTTTATGATTTTAAAAAAGCAGTTCTTAAATATA
ATAAAGAACATAGTTCTTTTGCTAATTTTGTAGAATTATCATATATTGAATTTAAAAAGAACAAATATAGATATGATTAT
TTTGTTCGTCAAGATGATGGAGTTCAATACTATTTAATCTATTATTTCTTAATTGATCAAAAAATTAGCAATCCAAATGA
AAATAAAATTATTTCATTAATTAAGTTCTTTGGTAAACAAATCAATAAAGAAAAAGCTTATCGTGTTCAAGAAAATCTTT
ATATGCATGTTAATGAAGATGATTTAATTAGTTTAATATCATATGATAAAAATGATGTTATTGATGATCCAAATATTATA
GAAAAACAAGCAGGTAATTAA

Upstream 100 bases:

>100_bases
CAATACGTCTTATCAAATTTTAAGATCGATGAATTGGTTATAATTAAACCAGCTATTATAAAAGGCGCTTTAGCTGCATT
AGAAGCGATTGGTGAAGATT

Downstream 100 bases:

>100_bases
TGGAAATTCGTGGTAAATTATTTGTTAGTCAAGTTGTTAGAAAATTTAATTTAAATGTTGTTGCAAACTCTGATTATATT
GATCGTGAAATTTCAACAAC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTKLWTNLINKITNKKYLAAVSGGPDSMAMLNMYKRNISVVCHVNYHKRESADRDQEIVVDFCKKNNLPIEILDVDEKVY
EKYAHIDNFQAKARLIRYDFFKEIGKKYNIQHLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVVNGMIVKRPVLFIR
KQTLQRYCDENKIKYGIDETNELDIYERNRVRKTISNWSLNEVYDFKKAVLKYNKEHSSFANFVELSYIEFKKNKYRYDY
FVRQDDGVQYYLIYYFLIDQKISNPNENKIISLIKFFGKQINKEKAYRVQENLYMHVNEDDLISLISYDKNDVIDDPNII
EKQAGN

Sequences:

>Translated_326_residues
MTKL*TNLINKITNKKYLAAVSGGPDSMAMLNMYKRNISVVCHVNYHKRESADRDQEIVVDFCKKNNLPIEILDVDEKVY
EKYAHIDNFQAKARLIRYDFFKEIGKKYNIQHLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVVNGMIVKRPVLFIR
KQTLQRYCDENKIKYGIDETNELDIYERNRVRKTISN*SLNEVYDFKKAVLKYNKEHSSFANFVELSYIEFKKNKYRYDY
FVRQDDGVQYYLIYYFLIDQKISNPNENKIISLIKFFGKQINKEKAYRVQENLYMHVNEDDLISLISYDKNDVIDDPNII
EKQAGN
>Mature_325_residues
TKL*TNLINKITNKKYLAAVSGGPDSMAMLNMYKRNISVVCHVNYHKRESADRDQEIVVDFCKKNNLPIEILDVDEKVYE
KYAHIDNFQAKARLIRYDFFKEIGKKYNIQHLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVVNGMIVKRPVLFIRK
QTLQRYCDENKIKYGIDETNELDIYERNRVRKTISN*SLNEVYDFKKAVLKYNKEHSSFANFVELSYIEFKKNKYRYDYF
VRQDDGVQYYLIYYFLIDQKISNPNENKIISLIKFFGKQINKEKAYRVQENLYMHVNEDDLISLISYDKNDVIDDPNIIE
KQAGN

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 38602; Mature: 38470

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLTNLINKITNKKYLAAVSGGPDSMAMLNMYKRNISVVCHVNYHKRESADRDQEIVVD
CCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCHHCCCCCCCHHHHHH
FCKKNNLPIEILDVDEKVYEKYAHIDNFQAKARLIRYDFFKEIGKKYNIQHLYIAHNFDD
HHHCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHCCCEEEEEECCHHH
FLETAYMQRARQSKALFYGIKESNVVNGMIVKRPVLFIRKQTLQRYCDENKIKYGIDETN
HHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
ELDIYERNRVRKTISNSLNEVYDFKKAVLKYNKEHSSFANFVELSYIEFKKNKYRYDYFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCEEEEEEE
RQDDGVQYYLIYYFLIDQKISNPNENKIISLIKFFGKQINKEKAYRVQENLYMHVNEDDL
ECCCCCEEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHEEECCEEEEECCHHH
ISLISYDKNDVIDDPNIIEKQAGN
HHHHHCCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
TKLTNLINKITNKKYLAAVSGGPDSMAMLNMYKRNISVVCHVNYHKRESADRDQEIVVD
CHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCHHCCCCCCCHHHHHH
FCKKNNLPIEILDVDEKVYEKYAHIDNFQAKARLIRYDFFKEIGKKYNIQHLYIAHNFDD
HHHCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHCCCEEEEEECCHHH
FLETAYMQRARQSKALFYGIKESNVVNGMIVKRPVLFIRKQTLQRYCDENKIKYGIDETN
HHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
ELDIYERNRVRKTISNSLNEVYDFKKAVLKYNKEHSSFANFVELSYIEFKKNKYRYDYFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCEEEEEEE
RQDDGVQYYLIYYFLIDQKISNPNENKIISLIKFFGKQINKEKAYRVQENLYMHVNEDDL
ECCCCCEEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHEEECCEEEEECCHHH
ISLISYDKNDVIDDPNIIEKQAGN
HHHHHCCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]