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The map label for this gene is yafJ [C]

Identifier: 209551921

GI number: 209551921

Start: 4519677

End: 4520489

Strand: Direct

Name: yafJ [C]

Synonym: Rleg2_4354

Alternate gene names: 209551921

Gene position: 4519677-4520489 (Clockwise)

Preceding gene: 209551916

Following gene: 209551924

Centisome position: 99.6

GC content: 65.44

Gene sequence:

>813_bases
ATGTGTCGCTGGGCAGCCTATCGCGGAGACCCTCTCTATCTCGAGGAGCTGGTATCCTCGCCCGCGCACTCGCTGATCGA
GCAGTCCCATTGCGCCACCCGCGCCAAGACGGCGACCAATGGCGACGGCTTCGGCATCGCCTGGTATGGCGACAGGCCCG
AGCCCGGCCGCTACCGCGATATCCTGCCGGCCTGGTCGGATTGCAATCTGAAGAGCCTGGCACGGCAGATCCGCTCGCCG
CTCTTTCTCGCCCATGTCCGCGCCGCTACCGGCGGCGGCACGCGCCGCGACAATTGCCACCCCTTCACCCATGACACCTG
GTCCTTCATGCACAACGGCCAGATCTCGGGCTTCGAGCGCCTGCGCCGGCCGATGGAGGCGATGCTCGACGACGAGTTGT
TCAACGCCCGCGGCGGCACCACCGATTCCGAGCTGATGTTCCTGCTGGCGCTGCAATTCGGCCTGCGCGAGGCGCCGATC
GCCGCGATGGCCCAGATGGTCGGTTTTGTCGAGGACCTGGCCGAAAGCATCCTCGGCTCGATCCTGCTGCGCTTTACCGC
CGCCTTCTCCGATGGCAAAACGCTTTATGCGATCCGCTATGCCACGGATCGCAAGGCGCCGACGCTCTATGCCTCACCTG
CAAGCGCCGGCTACTGCCTCGTTTCCGAGCCGTTGAACGACGATGTCGATGCCTGGGCTGAAATTCCCGATGGCAGCGCC
GTCACCGTCGGCAAGGATGGCGTTGAGGTCGTCGGTTTCCGGCCGGAAAAGCGGGGCGCCGCCAAGCCGCAGCGGGTGGC
TATTCCTGCATAA

Upstream 100 bases:

>100_bases
GCGCTTGCAAATCGGTGGCGCGTTAAGCTGTTATTAGCCGCGACGACAGACAATTTCAGCATGCCCGCCGGTGCGGCGGC
GGGTTCGGAGCAAGAAAGCC

Downstream 100 bases:

>100_bases
AGCGCGCCGCGATCAGCGCCGCCAGCCTTTCGGCAACCTCTTCCTTGGCGAGATCCGGCCATTGCTCGACGCCGTCACGG
CGAATGAGTTTGACGCTGTT

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MCRWAAYRGDPLYLEELVSSPAHSLIEQSHCATRAKTATNGDGFGIAWYGDRPEPGRYRDILPAWSDCNLKSLARQIRSP
LFLAHVRAATGGGTRRDNCHPFTHDTWSFMHNGQISGFERLRRPMEAMLDDELFNARGGTTDSELMFLLALQFGLREAPI
AAMAQMVGFVEDLAESILGSILLRFTAAFSDGKTLYAIRYATDRKAPTLYASPASAGYCLVSEPLNDDVDAWAEIPDGSA
VTVGKDGVEVVGFRPEKRGAAKPQRVAIPA

Sequences:

>Translated_270_residues
MCRWAAYRGDPLYLEELVSSPAHSLIEQSHCATRAKTATNGDGFGIAWYGDRPEPGRYRDILPAWSDCNLKSLARQIRSP
LFLAHVRAATGGGTRRDNCHPFTHDTWSFMHNGQISGFERLRRPMEAMLDDELFNARGGTTDSELMFLLALQFGLREAPI
AAMAQMVGFVEDLAESILGSILLRFTAAFSDGKTLYAIRYATDRKAPTLYASPASAGYCLVSEPLNDDVDAWAEIPDGSA
VTVGKDGVEVVGFRPEKRGAAKPQRVAIPA
>Mature_270_residues
MCRWAAYRGDPLYLEELVSSPAHSLIEQSHCATRAKTATNGDGFGIAWYGDRPEPGRYRDILPAWSDCNLKSLARQIRSP
LFLAHVRAATGGGTRRDNCHPFTHDTWSFMHNGQISGFERLRRPMEAMLDDELFNARGGTTDSELMFLLALQFGLREAPI
AAMAQMVGFVEDLAESILGSILLRFTAAFSDGKTLYAIRYATDRKAPTLYASPASAGYCLVSEPLNDDVDAWAEIPDGSA
VTVGKDGVEVVGFRPEKRGAAKPQRVAIPA

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324138, Length=299, Percent_Identity=32.4414715719064, Blast_Score=137, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017808
- InterPro:   IPR017932 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29586; Mature: 29586

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRWAAYRGDPLYLEELVSSPAHSLIEQSHCATRAKTATNGDGFGIAWYGDRPEPGRYRD
CCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHH
ILPAWSDCNLKSLARQIRSPLFLAHVRAATGGGTRRDNCHPFTHDTWSFMHNGQISGFER
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHH
LRRPMEAMLDDELFNARGGTTDSELMFLLALQFGLREAPIAAMAQMVGFVEDLAESILGS
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ILLRFTAAFSDGKTLYAIRYATDRKAPTLYASPASAGYCLVSEPLNDDVDAWAEIPDGSA
HHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCHHHHHCCCCCCE
VTVGKDGVEVVGFRPEKRGAAKPQRVAIPA
EEECCCCCEEEEECCCCCCCCCCCEECCCC
>Mature Secondary Structure
MCRWAAYRGDPLYLEELVSSPAHSLIEQSHCATRAKTATNGDGFGIAWYGDRPEPGRYRD
CCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHH
ILPAWSDCNLKSLARQIRSPLFLAHVRAATGGGTRRDNCHPFTHDTWSFMHNGQISGFER
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHH
LRRPMEAMLDDELFNARGGTTDSELMFLLALQFGLREAPIAAMAQMVGFVEDLAESILGS
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ILLRFTAAFSDGKTLYAIRYATDRKAPTLYASPASAGYCLVSEPLNDDVDAWAEIPDGSA
HHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCHHHHHCCCCCCE
VTVGKDGVEVVGFRPEKRGAAKPQRVAIPA
EEECCCCCEEEEECCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]