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The map label for this gene is prfC [H]

Identifier: 209551914

GI number: 209551914

Start: 4511669

End: 4513252

Strand: Direct

Name: prfC [H]

Synonym: Rleg2_4347

Alternate gene names: 209551914

Gene position: 4511669-4513252 (Clockwise)

Preceding gene: 209551911

Following gene: 209551916

Centisome position: 99.42

GC content: 63.38

Gene sequence:

>1584_bases
ATGGCCGAAAGTCTCGCCGAGGCGGTCTCCCGCCGCCGCACATTCGCTATTATCGCTCACCCGGACGCGGGCAAGACGAC
GCTCACCGAAAAGCTGCTGCTGTTCGGCGGCGCCATTCAGCTTGCCGGCGAAGTCAAGGCGAAGAAGGATCGTATGCAGA
CGCGCTCCGACTGGATGAAGATCGAGCGCGAGCGCGGCATCTCCGTCGTCACCTCGGTGATGACCTTCGAATATAACGAC
AATGTCTTCAACATCCTCGACACGCCCGGCCACGAGGATTTCGCCGACGACACCTATCGCACGCTGACGGCCGTCGACGC
CGCCGTCATGGTCATCGACGCCGCCAAAGGCATCGAGCCGCGCACGCTAAAACTTTTCGAAGTCTGCCGCATGCGCGATA
TCCCGATCATCACCTTCATCAACAAGATGGACCGCGAAAGCCGCGATCCCTTCGAGATTCTCGACGAGGTGGAAGAGAAG
CTGGCGCTGGATACGGCGCCGATCACCTGGCCGATCGGCCGCTCGAAAACCTTCTGCGGCTCCTACAATATTGCCGCCAA
CACGGTGCGCGGCTCGGATACCGAGATCGAGGGAACGCCGGTCAACGGCCCGCAGAGCGTTGCCGGCCGGCTGCCGGAAA
ACGAGCGCCAGGTCTTCGTCGAGGAGACCGAGCTGGCGATCGAGGCCTGCCGCCCCTTCGACCGCCAATCCTTCCTGGAA
GGCCATATGACGCCGGTCTTCTTCGGCTCGGCCCTGCGCAATTTCGGCGTTCGCGACCTGATCAACGCGCTCGGCGAATT
CGCGCCGCCGCCGCGCGACCAGGTTGCCGACACCAGGACCGTGCATGCGACCGACGACAAGATGACGGCCTTCGTCTTCA
AGATCCAGGCCAACATGGACCCCAACCATCGTGACCGCATCGCCTTTGCCCGCATCTGCTCCGGCAAGCTTGAGCGCGGC
ATGAAGGCGAGGCTTGCCCGCACCGGCAAGCAGCTCGGCCTGACGGCGCCGCAATTCTTCTTCGCCTCGCAGCGCCAGCT
CGCCGACACCGCCTATGCCGGCGATGTCGTCGGCATTCCCAATCACGGGACGCTGCGCATCGGCGATACGCTGACCGAAG
GCGAAGCCCTCGTCTTCCAGGGCGTGCCGAATTTCTCGCCCGAAATTCTGCGCCGCGTGCGCCTGGAAGATGCGATGAAG
GCGAAGAAGCTGAAGGAAGCCCTGCAGCAGATGGCCGAAGAAGGCGTCGTCCAGCTGTTCTCGCCTGAGGACGGCTCGCC
GGCAATCGTCGGCGTCGTCGGCGCGCTGCAGCTCGACGTCCTGAAGGAGCGGCTGATGGCCGAATACGGCCTGCCGGTCT
CCTTCGAAATGTCGCGCTTCTCCGTCTGCCGCTGGATCTCGGCCGATCAGCCGGCTGATCTGGAAAAATTCCTCACCGTC
AAACGCGGCGATATTGCCCGCGATCTCGACGGCGATCCGGTCTTCCTCGCCCAGGACGGTTTCTCGCTGCGTTACGAGTC
CGAACGCCATCCGGCGATCAAGATGGTCGCCATCAAGGAATATCACGCCGCCAAGGCGGCGTGA

Upstream 100 bases:

>100_bases
GAATGGTCATGGCCCATTACTTTGCGCAGCGAACGCCGAGGTCAATTGCAATGCCGGGCTTTAATCGCTAGATACGCGGC
AATTCCACAGGATTCATAGT

Downstream 100 bases:

>100_bases
TGCCCAAAGCAATTCTCGCGAGGCGATTCTAGCCGGCGAGCCGTTCCGCCAAAAAATCGACGACCGCGCGCACCGCCGGC
AGTTGGCCACGTCGGTGCGG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MAESLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRMQTRSDWMKIERERGISVVTSVMTFEYND
NVFNILDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEPRTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEK
LALDTAPITWPIGRSKTFCGSYNIAANTVRGSDTEIEGTPVNGPQSVAGRLPENERQVFVEETELAIEACRPFDRQSFLE
GHMTPVFFGSALRNFGVRDLINALGEFAPPPRDQVADTRTVHATDDKMTAFVFKIQANMDPNHRDRIAFARICSGKLERG
MKARLARTGKQLGLTAPQFFFASQRQLADTAYAGDVVGIPNHGTLRIGDTLTEGEALVFQGVPNFSPEILRRVRLEDAMK
AKKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLMAEYGLPVSFEMSRFSVCRWISADQPADLEKFLTV
KRGDIARDLDGDPVFLAQDGFSLRYESERHPAIKMVAIKEYHAAKAA

Sequences:

>Translated_527_residues
MAESLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRMQTRSDWMKIERERGISVVTSVMTFEYND
NVFNILDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEPRTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEK
LALDTAPITWPIGRSKTFCGSYNIAANTVRGSDTEIEGTPVNGPQSVAGRLPENERQVFVEETELAIEACRPFDRQSFLE
GHMTPVFFGSALRNFGVRDLINALGEFAPPPRDQVADTRTVHATDDKMTAFVFKIQANMDPNHRDRIAFARICSGKLERG
MKARLARTGKQLGLTAPQFFFASQRQLADTAYAGDVVGIPNHGTLRIGDTLTEGEALVFQGVPNFSPEILRRVRLEDAMK
AKKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLMAEYGLPVSFEMSRFSVCRWISADQPADLEKFLTV
KRGDIARDLDGDPVFLAQDGFSLRYESERHPAIKMVAIKEYHAAKAA
>Mature_526_residues
AESLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRMQTRSDWMKIERERGISVVTSVMTFEYNDN
VFNILDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEPRTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEKL
ALDTAPITWPIGRSKTFCGSYNIAANTVRGSDTEIEGTPVNGPQSVAGRLPENERQVFVEETELAIEACRPFDRQSFLEG
HMTPVFFGSALRNFGVRDLINALGEFAPPPRDQVADTRTVHATDDKMTAFVFKIQANMDPNHRDRIAFARICSGKLERGM
KARLARTGKQLGLTAPQFFFASQRQLADTAYAGDVVGIPNHGTLRIGDTLTEGEALVFQGVPNFSPEILRRVRLEDAMKA
KKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLMAEYGLPVSFEMSRFSVCRWISADQPADLEKFLTVK
RGDIARDLDGDPVFLAQDGFSLRYESERHPAIKMVAIKEYHAAKAA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=494, Percent_Identity=26.3157894736842, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI19923640, Length=502, Percent_Identity=26.0956175298805, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=27.8481012658228, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI25306287, Length=474, Percent_Identity=25.5274261603376, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=34.1935483870968, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=30.6122448979592, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=33.0434782608696, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=33.0434782608696, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=33.0434782608696, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.8724832214765, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790835, Length=523, Percent_Identity=48.7571701720841, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789738, Length=487, Percent_Identity=27.3100616016427, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=33.7579617834395, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.8993288590604, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=490, Percent_Identity=26.3265306122449, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=476, Percent_Identity=24.5798319327731, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=34.0909090909091, Blast_Score=89, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=481, Percent_Identity=26.6112266112266, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=513, Percent_Identity=25.3411306042885, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=29.2993630573248, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=29.746835443038, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=29.746835443038, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582462, Length=480, Percent_Identity=26.4583333333333, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI221458488, Length=503, Percent_Identity=26.0437375745527, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=31.875, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.0261437908497, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.0261437908497, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.0261437908497, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656849, Length=154, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58595; Mature: 58463

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRMQTRSDWMK
CCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHH
IERERGISVVTSVMTFEYNDNVFNILDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEP
HHHHCCHHEEEHEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCC
RTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEKLALDTAPITWPIGRSKTFCG
HHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCEECC
SYNIAANTVRGSDTEIEGTPVNGPQSVAGRLPENERQVFVEETELAIEACRPFDRQSFLE
CCCCEEEEECCCCCEECCCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHH
GHMTPVFFGSALRNFGVRDLINALGEFAPPPRDQVADTRTVHATDDKMTAFVFKIQANMD
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHCCCCCEEEECCCCEEEEEEEEEECCC
PNHRDRIAFARICSGKLERGMKARLARTGKQLGLTAPQFFFASQRQLADTAYAGDVVGIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCEEECC
NHGTLRIGDTLTEGEALVFQGVPNFSPEILRRVRLEDAMKAKKLKEALQQMAEEGVVQLF
CCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SPEDGSPAIVGVVGALQLDVLKERLMAEYGLPVSFEMSRFSVCRWISADQPADLEKFLTV
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHH
KRGDIARDLDGDPVFLAQDGFSLRYESERHPAIKMVAIKEYHAAKAA
HCCCCCCCCCCCEEEEECCCCEEEECCCCCCCEEEEEEHHHHHHCCC
>Mature Secondary Structure 
AESLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRMQTRSDWMK
CHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHH
IERERGISVVTSVMTFEYNDNVFNILDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEP
HHHHCCHHEEEHEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCC
RTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEKLALDTAPITWPIGRSKTFCG
HHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCEECC
SYNIAANTVRGSDTEIEGTPVNGPQSVAGRLPENERQVFVEETELAIEACRPFDRQSFLE
CCCCEEEEECCCCCEECCCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHH
GHMTPVFFGSALRNFGVRDLINALGEFAPPPRDQVADTRTVHATDDKMTAFVFKIQANMD
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHCCCCCEEEECCCCEEEEEEEEEECCC
PNHRDRIAFARICSGKLERGMKARLARTGKQLGLTAPQFFFASQRQLADTAYAGDVVGIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCEEECC
NHGTLRIGDTLTEGEALVFQGVPNFSPEILRRVRLEDAMKAKKLKEALQQMAEEGVVQLF
CCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SPEDGSPAIVGVVGALQLDVLKERLMAEYGLPVSFEMSRFSVCRWISADQPADLEKFLTV
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHH
KRGDIARDLDGDPVFLAQDGFSLRYESERHPAIKMVAIKEYHAAKAA
HCCCCCCCCCCCEEEEECCCCEEEECCCCCCCEEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA