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The map label for this gene is rsmB [H]

Identifier: 209551883

GI number: 209551883

Start: 4482919

End: 4484208

Strand: Reverse

Name: rsmB [H]

Synonym: Rleg2_4316

Alternate gene names: 209551883

Gene position: 4484208-4482919 (Counterclockwise)

Preceding gene: 209551884

Following gene: 209551882

Centisome position: 98.82

GC content: 66.98

Gene sequence:

>1290_bases
ATGCGTCTGGGCGGCCGCCTCGAAGGGGCGATTTCTGTGCTCGCGGATATCGATGCCCGCAAGCGGCCCGTCGCCGACGC
GCTGAAGGACTGGGGCCTTGCGCACCGCTTCGCCGGCTCCGGCGACCGCGCCGCCATCGGCAACATCGTCTATGACGCTC
TGCGCATGCGGCTTTCCCATGCCTGGCTGATGGATGACGACAGTGCCGCGGCCATTGCCCATGCCGTCATGTTCCGCCAA
TGGGGTTTCACACCGGACAGTCTCGCCGCCGAACTGGCGGACGACAAGTTCGCGCCGGCACCGCTTTCGGCCGATGCCCT
CGCGGCCTTCGAAAGCCGCTCGCTCGACGATGCGCCGCCGCATATCCGCGGCGATATTCCCGAATGGGTCCAATCCTCCT
TCGAACAGGCCTTCGGCGCCGGCTGGCTTACCGAGGCACAGGCACTTGCGGCGCGGCCGACACTCGATCTGCGCGCCAAC
ATCCTGAAGTCCTCCCGCGACAAGGCCGTCAAGGCGCTCGAAAGAGCCGGCGCGCAAGCGGCGAAAGTCGCCCGCTACGG
CATCCGGATTGCCGCCGGCGAAGGCGCCTCGCGCCTACCCAACGTGACGGCCGAGCTTTCGTTCCAAAAGGGCTGGTTCG
AAGTTCAGGATGAAGGATCGCAGATCGTCGCCGACCTGGTGCTCGCCCATGAGGGCGACCAGGTTCTCGACTATTGCGCC
GGCGGCGGCGGCAAGACGCTCGCCATGGCGGCCGCCATGCAGAATAAGGGACAGGTTCACGCTTACGACGCCGACCGCAA
ACGGCTGGCGCCGATCATCGAACGGCTGAAGCGGGCGGGCACGCGCAATGTCCAGGTGCATGACGACGCCAGGGGCCTTT
CCGGGCTTGCCGGCCGCTGCGAGAAGGTGCTGGTCGACGCGCCCTGCACCGGCACCGGCACGTGGCGCCGCCGACCCGAT
ACGAAATGGCGGCTGACGGCGAAGAACCTCGACGAACGCACCGCCCAGCAGCAGGACGCACTGGCGCAGGCAAGCGGCTT
CGTCAAGCCGGGCGGCGAGTTGATCTACGTCACCTGCTCGGTGCTGCCGCAGGAGAACGAGGAACAGGTGCGCCGCTTCA
CGGCTGACAATCCCGATTTCCAGATCGTCAGCGCCCTACCCGCCTGGGATGGCCTCTTCGGCAAAGACGCCCCACGGCCG
CACTCTTCCGATGGCCTGACGGTGACGCTGACGCCGGCTTCCACCGACACGGACGGCTTCTTCTTCTGTCGCATGCAGCG
GAAGGGCTGA

Upstream 100 bases:

>100_bases
AATCGCGGTTACCACGCGGTTGAGACTTGTGAACACTCCGCCCACGCCTGTCGTGACCATGCGGACCGATCGCACGTCAT
GGCAATTAGGAGATTTGTGA

Downstream 100 bases:

>100_bases
CCCGCGGCGAAACGGCGCGGCAGCTGCCGATTTTCCGGACGAATGCCCTGCAAAACGAGCGCGTTTGTGGCCCTCCTCCA
TCCTTAAAACCATTCCAAAC

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRLGGRLEGAISVLADIDARKRPVADALKDWGLAHRFAGSGDRAAIGNIVYDALRMRLSHAWLMDDDSAAAIAHAVMFRQ
WGFTPDSLAAELADDKFAPAPLSADALAAFESRSLDDAPPHIRGDIPEWVQSSFEQAFGAGWLTEAQALAARPTLDLRAN
ILKSSRDKAVKALERAGAQAAKVARYGIRIAAGEGASRLPNVTAELSFQKGWFEVQDEGSQIVADLVLAHEGDQVLDYCA
GGGGKTLAMAAAMQNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHDDARGLSGLAGRCEKVLVDAPCTGTGTWRRRPD
TKWRLTAKNLDERTAQQQDALAQASGFVKPGGELIYVTCSVLPQENEEQVRRFTADNPDFQIVSALPAWDGLFGKDAPRP
HSSDGLTVTLTPASTDTDGFFFCRMQRKG

Sequences:

>Translated_429_residues
MRLGGRLEGAISVLADIDARKRPVADALKDWGLAHRFAGSGDRAAIGNIVYDALRMRLSHAWLMDDDSAAAIAHAVMFRQ
WGFTPDSLAAELADDKFAPAPLSADALAAFESRSLDDAPPHIRGDIPEWVQSSFEQAFGAGWLTEAQALAARPTLDLRAN
ILKSSRDKAVKALERAGAQAAKVARYGIRIAAGEGASRLPNVTAELSFQKGWFEVQDEGSQIVADLVLAHEGDQVLDYCA
GGGGKTLAMAAAMQNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHDDARGLSGLAGRCEKVLVDAPCTGTGTWRRRPD
TKWRLTAKNLDERTAQQQDALAQASGFVKPGGELIYVTCSVLPQENEEQVRRFTADNPDFQIVSALPAWDGLFGKDAPRP
HSSDGLTVTLTPASTDTDGFFFCRMQRKG
>Mature_429_residues
MRLGGRLEGAISVLADIDARKRPVADALKDWGLAHRFAGSGDRAAIGNIVYDALRMRLSHAWLMDDDSAAAIAHAVMFRQ
WGFTPDSLAAELADDKFAPAPLSADALAAFESRSLDDAPPHIRGDIPEWVQSSFEQAFGAGWLTEAQALAARPTLDLRAN
ILKSSRDKAVKALERAGAQAAKVARYGIRIAAGEGASRLPNVTAELSFQKGWFEVQDEGSQIVADLVLAHEGDQVLDYCA
GGGGKTLAMAAAMQNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHDDARGLSGLAGRCEKVLVDAPCTGTGTWRRRPD
TKWRLTAKNLDERTAQQQDALAQASGFVKPGGELIYVTCSVLPQENEEQVRRFTADNPDFQIVSALPAWDGLFGKDAPRP
HSSDGLTVTLTPASTDTDGFFFCRMQRKG

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=291, Percent_Identity=29.8969072164948, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI76150623, Length=291, Percent_Identity=29.8969072164948, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI8922322, Length=236, Percent_Identity=32.2033898305085, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI270288818, Length=236, Percent_Identity=32.2033898305085, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI270288816, Length=236, Percent_Identity=32.2033898305085, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI23199998, Length=236, Percent_Identity=32.2033898305085, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI32698918, Length=198, Percent_Identity=28.2828282828283, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI2367212, Length=306, Percent_Identity=30.0653594771242, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081985, Length=221, Percent_Identity=28.5067873303167, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=287, Percent_Identity=31.0104529616725, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324268, Length=294, Percent_Identity=29.5918367346939, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI22024126, Length=290, Percent_Identity=26.8965517241379, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356579, Length=222, Percent_Identity=29.7297297297297, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24668781, Length=267, Percent_Identity=26.9662921348315, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356513, Length=267, Percent_Identity=26.9662921348315, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21355201, Length=182, Percent_Identity=29.6703296703297, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46374; Mature: 46374

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGGRLEGAISVLADIDARKRPVADALKDWGLAHRFAGSGDRAAIGNIVYDALRMRLSH
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AWLMDDDSAAAIAHAVMFRQWGFTPDSLAAELADDKFAPAPLSADALAAFESRSLDDAPP
HEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCC
HIRGDIPEWVQSSFEQAFGAGWLTEAQALAARPTLDLRANILKSSRDKAVKALERAGAQA
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
AKVARYGIRIAAGEGASRLPNVTAELSFQKGWFEVQDEGSQIVADLVLAHEGDQVLDYCA
HHHHHHCEEEEECCCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHCCCCHHHHHHC
GGGGKTLAMAAAMQNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHDDARGLSGLAGRC
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHC
EKVLVDAPCTGTGTWRRRPDTKWRLTAKNLDERTAQQQDALAQASGFVKPGGELIYVTCS
CEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
VLPQENEEQVRRFTADNPDFQIVSALPAWDGLFGKDAPRPHSSDGLTVTLTPASTDTDGF
ECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
FFCRMQRKG
EEEEEECCC
>Mature Secondary Structure
MRLGGRLEGAISVLADIDARKRPVADALKDWGLAHRFAGSGDRAAIGNIVYDALRMRLSH
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AWLMDDDSAAAIAHAVMFRQWGFTPDSLAAELADDKFAPAPLSADALAAFESRSLDDAPP
HEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCC
HIRGDIPEWVQSSFEQAFGAGWLTEAQALAARPTLDLRANILKSSRDKAVKALERAGAQA
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
AKVARYGIRIAAGEGASRLPNVTAELSFQKGWFEVQDEGSQIVADLVLAHEGDQVLDYCA
HHHHHHCEEEEECCCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHCCCCHHHHHHC
GGGGKTLAMAAAMQNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHDDARGLSGLAGRC
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHC
EKVLVDAPCTGTGTWRRRPDTKWRLTAKNLDERTAQQQDALAQASGFVKPGGELIYVTCS
CEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
VLPQENEEQVRRFTADNPDFQIVSALPAWDGLFGKDAPRPHSSDGLTVTLTPASTDTDGF
ECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
FFCRMQRKG
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA